BLASTX nr result
ID: Coptis25_contig00014862
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00014862
(1268 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002331746.1| predicted protein [Populus trichocarpa] gi|2... 76 2e-39
ref|XP_002331338.1| predicted protein [Populus trichocarpa] gi|2... 99 3e-32
ref|NP_175044.1| DNAse I-like superfamily protein [Arabidopsis t... 65 1e-24
gb|AAF63113.1|AC006423_14 Hypothetical protein [Arabidopsis thal... 65 1e-23
ref|XP_002332412.1| predicted protein [Populus trichocarpa] gi|2... 96 7e-23
>ref|XP_002331746.1| predicted protein [Populus trichocarpa] gi|222874272|gb|EEF11403.1|
predicted protein [Populus trichocarpa]
Length = 503
Score = 76.3 bits (186), Expect(4) = 2e-39
Identities = 50/163 (30%), Positives = 76/163 (46%), Gaps = 1/163 (0%)
Frame = +2
Query: 221 DLKFTGFFYTWSNNSDGRNKIMSKIDRCVVNDKWLAEFGSSEAEFLPYGILDHNPVLIS- 397
DL+++G YTWSN N IM K+DR +VN+KW F SE FLP GI DH+P+++
Sbjct: 160 DLRYSGMHYTWSNQCP-ENLIMRKLDRVLVNEKWNLNFPLSEVRFLPSGISDHSPMVVKV 218
Query: 398 WADYKRKASPFRFFNNWVFHTEFKDILEDAWLQYVDSNPIYILMQXXXXXXXXXXXXXXX 577
+ + PFRFF+ W+ Q P+Y L
Sbjct: 219 IGNDQNIKKPFRFFDMWMD-------------QNSGGCPMYQLCCNLKKLKQELKLFNMA 265
Query: 578 HFSSLHSQLEVTRTCLYDMQQKLQLHPLDITLILKERTEMKKY 706
HFS++ +++ + + QQ L + L ++ER + KY
Sbjct: 266 HFSNISDRVKDAKNEMDKAQQALHTAHENPILCMRERDVVHKY 308
Score = 75.5 bits (184), Expect(4) = 2e-39
Identities = 45/134 (33%), Positives = 68/134 (50%), Gaps = 6/134 (4%)
Frame = +1
Query: 721 KAEFMELKQKNSNDWFVDGDQGTTYFFNCIKEKKNKHATWSLVSNEGVRLDTQQLVTNEV 900
+AE KQK W GDQ T+YF + + N++ SL +G ++ + V +EV
Sbjct: 313 RAEESFFKQKARIQWLSLGDQNTSYFHKSVNGRHNRNKLLSLTREDGEVVEGHEAVKSEV 372
Query: 901 VGYYKNLIGTSSAITVGHEFFSDLNISTSLSSGVADSLAVDVT------PLFSMNDDKSP 1062
+ Y+ ++G V +E + I+ LSS LA VT +FS+ ++K+P
Sbjct: 373 IAYFHRVLGVDQMPRVLNEEVMESAINLKLSSTQQHVLAQVVTRKEIKHAMFSLKNNKAP 432
Query: 1063 GPDGFNAFFFKTCW 1104
G DGFNA FFK W
Sbjct: 433 GLDGFNAGFFKRMW 446
Score = 40.4 bits (93), Expect(4) = 2e-39
Identities = 20/52 (38%), Positives = 32/52 (61%)
Frame = +3
Query: 1110 VGETFTNAIFNVFSSGHFSRGVNATTLCLIPKTSNPTRVGNIDLSPVVLLST 1265
VGE NA+ ++F + + +NAT++ LIPK +NPTR+ D P+ +T
Sbjct: 449 VGEDVINAVRSLFQTRRMLKEMNATSISLIPKVANPTRL--TDFRPISCCNT 498
Score = 38.9 bits (89), Expect(4) = 2e-39
Identities = 18/47 (38%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
Frame = +3
Query: 9 VYARNDGSARLELWRDLTSLQVS--HIPWCVFGDFNNVTNAHERVGG 143
+Y N+ S R LW D+ S PW + GDFN + N R+GG
Sbjct: 92 IYGDNNASLREALWSDIVSRSDGWESTPWILMGDFNAIRNQSHRLGG 138
>ref|XP_002331338.1| predicted protein [Populus trichocarpa] gi|222873371|gb|EEF10502.1|
predicted protein [Populus trichocarpa]
Length = 819
Score = 99.0 bits (245), Expect(3) = 3e-32
Identities = 55/163 (33%), Positives = 85/163 (52%), Gaps = 1/163 (0%)
Frame = +2
Query: 221 DLKFTGFFYTWSNNSDGRNKIMSKIDRCVVNDKWLAEFGSSEAEFLPYGILDHNPVLIS- 397
DL+++G YTWSN N IM K+DR +VN+KW +F SEA FLP G+ DH+P+++
Sbjct: 553 DLRYSGMHYTWSNQCP-ENLIMRKLDRVLVNEKWNLKFPLSEARFLPSGMSDHSPMVVKV 611
Query: 398 WADYKRKASPFRFFNNWVFHTEFKDILEDAWLQYVDSNPIYILMQXXXXXXXXXXXXXXX 577
+ + K PFRFF+ W+ H EF +++ W Q P+Y L
Sbjct: 612 IGNDQNKKKPFRFFDMWMDHDEFMPLVKKVWDQNSRGCPMYQLCCKLRKLKQELKLFNMA 671
Query: 578 HFSSLHSQLEVTRTCLYDMQQKLQLHPLDITLILKERTEMKKY 706
HFS++ ++ + + QQ L + L ++ER + KY
Sbjct: 672 HFSNISDRVRDAKNKMDKAQQALHTAHENPILCMRERDVVHKY 714
Score = 52.0 bits (123), Expect(3) = 3e-32
Identities = 28/92 (30%), Positives = 47/92 (51%)
Frame = +1
Query: 721 KAEFMELKQKNSNDWFVDGDQGTTYFFNCIKEKKNKHATWSLVSNEGVRLDTQQLVTNEV 900
+AE KQK W GDQ T+YF + ++N++ SL +G ++ Q+ V +EV
Sbjct: 719 RAEESFFKQKARIQWLSLGDQNTSYFHKSVNGRQNRNKLLSLTREDGEVVERQEAVKSEV 778
Query: 901 VGYYKNLIGTSSAITVGHEFFSDLNISTSLSS 996
+ Y+ ++G V +E + I+ LSS
Sbjct: 779 ISYFHRVLGVDQMPRVLNEEVMESAINLKLSS 810
Score = 35.8 bits (81), Expect(3) = 3e-32
Identities = 17/47 (36%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
Frame = +3
Query: 9 VYARNDGSARLELWRDLTSLQVS--HIPWCVFGDFNNVTNAHERVGG 143
+Y N+ S R LW D+ S W + GDFN + N +R+GG
Sbjct: 485 IYGDNNASLREALWSDIVSRSDGWESTLWILIGDFNAIRNQSDRLGG 531
>ref|NP_175044.1| DNAse I-like superfamily protein [Arabidopsis thaliana]
gi|332193872|gb|AEE31993.1| DNAse I-like superfamily
protein [Arabidopsis thaliana]
Length = 626
Score = 65.1 bits (157), Expect(4) = 1e-24
Identities = 48/169 (28%), Positives = 76/169 (44%), Gaps = 2/169 (1%)
Frame = +2
Query: 215 LVDLKFTGFFYTWSNNSDGRNKIMSKIDRCVVNDKWLAEFGSSEAEFLPYGILDHNPVLI 394
LVD+ G YTWSN+ D N I+ K+DR + N W + F S+ A F G+ DH+P +I
Sbjct: 261 LVDIPSRGVHYTWSNHQDD-NPIIRKLDRAIANGDWFSSFPSAIAVFELSGVSDHSPCII 319
Query: 395 SWADY-KRKASPFRFFNNWVFHTEFKDILEDAWLQYVD-SNPIYILMQXXXXXXXXXXXX 568
+ KR FR+F+ H F L AW + + + ++ L +
Sbjct: 320 ILENLPKRSKKCFRYFSFLSTHPTFLVSLTVAWEEQIPVGSHMFSLGEHLKAAKKCCKLL 379
Query: 569 XXXHFSSLHSQLEVTRTCLYDMQQKLQLHPLDITLILKERTEMKKYGDF 715
F ++ + + L +Q +L +P D +L E KK+ F
Sbjct: 380 NRQGFGNIQHKTKEALDSLESIQSQLLTNPSD-SLFRVEHVARKKWNFF 427
Score = 62.0 bits (149), Expect(4) = 1e-24
Identities = 43/143 (30%), Positives = 68/143 (47%), Gaps = 10/143 (6%)
Frame = +1
Query: 706 W*FLAKAEFMELKQKNSNDWFVDGDQGTTYFFNCIKEKKNKHATWSLVSNEGVRLDTQQL 885
W F A A +QK+ W DGD T +F I + K+ L ++ VR++
Sbjct: 424 WNFFAAALESFYRQKSRIKWLQDGDANTRFFHKVILANQAKNLIKFLRMDDDVRVENVTQ 483
Query: 886 VTNEVVGYYKNLIGTSSAITVG---------HEFFSDLNISTSLSSGVADSLAVDVT-PL 1035
V +V YY +L+G+ S I H F + +++ LS+ +D ++T +
Sbjct: 484 VKEMIVAYYTHLLGSDSDILTPDSVQRIKDIHPFRCNDTLASRLSALPSDK---EITAAV 540
Query: 1036 FSMNDDKSPGPDGFNAFFFKTCW 1104
F+M +K+PGPD F A FF W
Sbjct: 541 FAMPRNKAPGPDSFTAEFFWESW 563
Score = 26.6 bits (57), Expect(4) = 1e-24
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +3
Query: 1131 AIFNVFSSGHFSRGVNATTLCLIPKTS 1211
A+ F +GH + NAT + LIPK +
Sbjct: 573 AVKEFFRTGHLLKRFNATAITLIPKVT 599
Score = 26.6 bits (57), Expect(4) = 1e-24
Identities = 6/12 (50%), Positives = 12/12 (100%)
Frame = +2
Query: 1232 YRPIACCTIIYR 1267
+RP++CCT++Y+
Sbjct: 607 FRPVSCCTVVYK 618
>gb|AAF63113.1|AC006423_14 Hypothetical protein [Arabidopsis thaliana]
Length = 668
Score = 65.1 bits (157), Expect(4) = 1e-23
Identities = 48/169 (28%), Positives = 76/169 (44%), Gaps = 2/169 (1%)
Frame = +2
Query: 215 LVDLKFTGFFYTWSNNSDGRNKIMSKIDRCVVNDKWLAEFGSSEAEFLPYGILDHNPVLI 394
LVD+ G YTWSN+ D N I+ K+DR + N W + F S+ A F G+ DH+P +I
Sbjct: 197 LVDIPSRGVHYTWSNHQDD-NPIIRKLDRAIANGDWFSSFPSAIAVFELSGVSDHSPCII 255
Query: 395 SWADY-KRKASPFRFFNNWVFHTEFKDILEDAWLQYVD-SNPIYILMQXXXXXXXXXXXX 568
+ KR FR+F+ H F L AW + + + ++ L +
Sbjct: 256 ILENLPKRSKKCFRYFSFLSTHPTFLVSLTVAWEEQIPVGSHMFSLGEHLKAAKKCCKLL 315
Query: 569 XXXHFSSLHSQLEVTRTCLYDMQQKLQLHPLDITLILKERTEMKKYGDF 715
F ++ + + L +Q +L +P D +L E KK+ F
Sbjct: 316 NRQGFGNIQHKTKEALDSLESIQSQLLTNPSD-SLFRVEHVARKKWNFF 363
Score = 62.0 bits (149), Expect(4) = 1e-23
Identities = 43/143 (30%), Positives = 68/143 (47%), Gaps = 10/143 (6%)
Frame = +1
Query: 706 W*FLAKAEFMELKQKNSNDWFVDGDQGTTYFFNCIKEKKNKHATWSLVSNEGVRLDTQQL 885
W F A A +QK+ W DGD T +F I + K+ L ++ VR++
Sbjct: 360 WNFFAAALESFYRQKSRIKWLQDGDANTRFFHKVILANQAKNLIKFLRMDDDVRVENVTQ 419
Query: 886 VTNEVVGYYKNLIGTSSAITVG---------HEFFSDLNISTSLSSGVADSLAVDVT-PL 1035
V +V YY +L+G+ S I H F + +++ LS+ +D ++T +
Sbjct: 420 VKEMIVAYYTHLLGSDSDILTPDSVQRIKDIHPFRCNDTLASRLSALPSDK---EITAAV 476
Query: 1036 FSMNDDKSPGPDGFNAFFFKTCW 1104
F+M +K+PGPD F A FF W
Sbjct: 477 FAMPRNKAPGPDSFTAEFFWESW 499
Score = 26.6 bits (57), Expect(4) = 1e-23
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +3
Query: 1131 AIFNVFSSGHFSRGVNATTLCLIPKTS 1211
A+ F +GH + NAT + LIPK +
Sbjct: 509 AVKEFFRTGHLLKRFNATAITLIPKVT 535
Score = 23.1 bits (48), Expect(4) = 1e-23
Identities = 5/10 (50%), Positives = 10/10 (100%)
Frame = +2
Query: 1232 YRPIACCTII 1261
+RP++CCT++
Sbjct: 543 FRPVSCCTVV 552
>ref|XP_002332412.1| predicted protein [Populus trichocarpa] gi|222832345|gb|EEE70822.1|
predicted protein [Populus trichocarpa]
Length = 349
Score = 96.3 bits (238), Expect(2) = 7e-23
Identities = 55/163 (33%), Positives = 84/163 (51%), Gaps = 1/163 (0%)
Frame = +2
Query: 221 DLKFTGFFYTWSNNSDGRNKIMSKIDRCVVNDKWLAEFGSSEAEFLPYGILDHNPVLIS- 397
DL ++G YTWSN N IM K+DR +VN+KW F SEA FLP G+ DH+P+++
Sbjct: 176 DLWYSGMHYTWSNQCP-ENLIMRKLDRVLVNEKWNLNFPLSEARFLPSGMSDHSPMVVKV 234
Query: 398 WADYKRKASPFRFFNNWVFHTEFKDILEDAWLQYVDSNPIYILMQXXXXXXXXXXXXXXX 577
+ + PFRFF+ W+ H EF +++ W Q P+Y L
Sbjct: 235 IGNDQNIKKPFRFFDMWMDHDEFMPLVKKVWDQNSGGCPMYQLCCKLRKQKQELKLFNMA 294
Query: 578 HFSSLHSQLEVTRTCLYDMQQKLQLHPLDITLILKERTEMKKY 706
HFS++ +++ + + QQ L + TL ++ER + KY
Sbjct: 295 HFSNISDRVKDAKNEMDKAQQALHTAHKNPTLCMQERDVVHKY 337
Score = 38.5 bits (88), Expect(2) = 7e-23
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Frame = +3
Query: 9 VYARNDGSARLELWRDLTSLQVS--HIPWCVFGDFNNVTNAHERVGG 143
+Y N+ S R LW D+ S PW + GDFN + N +++GG
Sbjct: 108 IYGDNNASLREALWSDIVSRSDGWESTPWILMGDFNAIRNQSDKLGG 154