BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00014058
         (1549 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]   375   e-101
gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]             374   e-101
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   303   1e-79
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap...   294   4e-77
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   292   2e-76

>gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]
          Length = 356

 Score =  375 bits (962), Expect = e-101
 Identities = 184/349 (52%), Positives = 255/349 (73%), Gaps = 4/349 (1%)
 Frame = +2

Query: 92   DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIA----LSSLATSPTLVSI 259
            +MK  +QI N +CG VD+ VL+CA++L +FD I N+   KP+     LSS+ +SP+  SI
Sbjct: 11   EMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNS--GKPMITLTELSSIVSSPSSSSI 68

Query: 260  KPNNLYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLLRDQNRSLVDWALAIIDETVID 439
            +P NLYRL+RYL  M+L++I    N+ T SLT  SKLLLR+Q +SL+DW LAI  E ++ 
Sbjct: 69   EPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLAIXCEMMVV 128

Query: 440  GWHELSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCC 619
             WHELS   ++P   P  F++VHGK+  ELAGE    N +IN+AM SDT +  P  +Q C
Sbjct: 129  VWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGC 188

Query: 620  DKLLNGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFK 799
             K+LNG+TS++D+GGG G TM+YIV+AFPHIK  V DLPHV+ ++ + PGVE + GD+FK
Sbjct: 189  GKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFK 248

Query: 800  FIPPADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAK 979
             I  ADA+ LK++LHNW D EC+ +LK+CKE +P DKGKVII+D+V D ++ +  LT+AK
Sbjct: 249  SISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVID-DDDNSILTQAK 307

Query: 980  MNLDIDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 1126
            ++LD+ +M   GGRERT+ +W  L++++GFSRHEIIPI A+ S+IVAYP
Sbjct: 308  LSLDLTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356


>gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]
          Length = 356

 Score =  374 bits (961), Expect = e-101
 Identities = 184/349 (52%), Positives = 255/349 (73%), Gaps = 4/349 (1%)
 Frame = +2

Query: 92   DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIA----LSSLATSPTLVSI 259
            +MK  +QI N +CG VD+ VL+CA++L +FD I N+   KP+     LSS+ +SP+  SI
Sbjct: 11   EMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNS--GKPMITLTELSSIVSSPSSSSI 68

Query: 260  KPNNLYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLLRDQNRSLVDWALAIIDETVID 439
            +P NLYRL+RYL  M+L++I    N+ T SLT  SKLLLR+Q +SL+DW LAI  E ++ 
Sbjct: 69   EPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLAISCEMMVV 128

Query: 440  GWHELSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCC 619
             WHELS   ++P   P  F++VHGK+  ELAGE    N +IN+AM SDT +  P  +Q C
Sbjct: 129  VWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGC 188

Query: 620  DKLLNGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFK 799
             K+LNG+TS++D+GGG G TM+YIV+AFPHIK  V DLPHV+ ++ + PGVE + GD+FK
Sbjct: 189  GKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFK 248

Query: 800  FIPPADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAK 979
             I  ADA+ LK++LHNW D EC+ +LK+CKE +P DKGKVII+D+V D ++ +  LT+AK
Sbjct: 249  SISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVID-DDDNSILTQAK 307

Query: 980  MNLDIDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 1126
            ++LD+ +M   GGRERT+ +W  L++++GFSRHEIIPI A+ S+IVAYP
Sbjct: 308  LSLDLTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase 2 [Papaver somniferum]
          Length = 357

 Score =  303 bits (775), Expect = 1e-79
 Identities = 153/346 (44%), Positives = 224/346 (64%), Gaps = 2/346 (0%)
 Frame = +2

Query: 95   MKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIALSSLATSPTLVSIKPNNL 274
            +K  AQ+ N + G  DS+VL+CAVE+ + D+I NN  +  I L+ LA    + ++  + L
Sbjct: 17   IKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGA--ITLAQLAAKLPITNVSSDYL 74

Query: 275  YRLLRYLVHMNLLTIHV--EGNDETFSLTELSKLLLRDQNRSLVDWALAIIDETVIDGWH 448
            YR++RYLVH+N++       G ++ +SL  +  LLLRD  RS+V   L +  +  +  WH
Sbjct: 75   YRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH 134

Query: 449  ELSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKL 628
             +     +  G  T FE+  G  +W+    N   +Q+ N+ M  +T L+    ++ C   
Sbjct: 135  FMKEGLGN--GSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDT 192

Query: 629  LNGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFKFIP 808
              G+ S+VDIGGG G T+  I +AFPHIKCT++DLPHV+A+S  LP +E V GDMFK +P
Sbjct: 193  FQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVP 252

Query: 809  PADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMNL 988
             A AI LK +LH+W D+EC+ ILKKCKE IP++ GKVII+D+  ++ E + +LT+ ++ L
Sbjct: 253  SAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEE-ESNHELTKTRLIL 311

Query: 989  DIDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 1126
            DIDM+V +GGRERT ++WE LLK AGF  H+I PI A+QSVI A+P
Sbjct: 312  DIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357


>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 346

 Score =  294 bits (753), Expect = 4e-77
 Identities = 155/347 (44%), Positives = 220/347 (63%), Gaps = 2/347 (0%)
 Frame = +2

Query: 92   DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIALSSLATS-PTLVSIKPN 268
            D ++ A+I   + G  +S+VLKCAV+L + + + NN   KP++LS LA+  P    +  +
Sbjct: 8    DQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNV--KPMSLSELASKLPVPQPVNED 65

Query: 269  NLYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLLRDQNRSLVDWALAIIDETVIDGWH 448
             L+R++RYLVHM L  I  +   + +SL   +K LLR   +S+VD  L I D+  +  WH
Sbjct: 66   RLFRIMRYLVHMELFKI--DATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWH 123

Query: 449  ELSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKL 628
             L       TG    FE+  GKS+W    EN   NQ+ N AM  DT LV       C  +
Sbjct: 124  HLGD---GLTGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI 180

Query: 629  LN-GITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFKFI 805
             + GI ++VD+GGG G  +  I KAFP IKCT++DLPHVIA S ++P +  + GDMFK I
Sbjct: 181  FSDGIKTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240

Query: 806  PPADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMN 985
            P ADAIF+K +LH+W+D+ECI ILK+CKE +P+D GKVII+D+V D  +      + ++ 
Sbjct: 241  PSADAIFMKCILHDWNDEECIQILKRCKEALPKD-GKVIIVDVVIDM-DSTHPYAKIRLT 298

Query: 986  LDIDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 1126
            LD+DMM+ +GG+ERT+ EW++L   AGF+ H++  I AVQSVI AYP
Sbjct: 299  LDLDMMLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYP 345


>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  292 bits (747), Expect = 2e-76
 Identities = 153/345 (44%), Positives = 220/345 (63%)
 Frame = +2

Query: 92   DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDSKPIALSSLATSPTLVSIKPNN 271
            ++ S A++ N + G  +S+VLKCAV+L+L ++I N+  S  + LS L++      +  + 
Sbjct: 7    NLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTS--MTLSELSSRLPSQPVNEDA 64

Query: 272  LYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLLRDQNRSLVDWALAIIDETVIDGWHE 451
            LYR++RYLVHM L T      +  + L   +K L++  ++ +V   LAI D+  +  WH 
Sbjct: 65   LYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWHY 124

Query: 452  LSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKLL 631
            L    +  +G  T FE+  G ++W    E+   NQ+ N+AM +D+ L+M   V+ C  + 
Sbjct: 125  LKDGLSGESG--TAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIF 182

Query: 632  NGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFKFIPP 811
            NGIT++VD+GGG G  +  I  AFPHIKCTV+DLPHVIA S     V  V GDMFKFIP 
Sbjct: 183  NGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIPK 242

Query: 812  ADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMNLD 991
            ADAI +K +LH+W DKECI ILK+CKE +P   GKVII+DIV +  + +   T+ ++ LD
Sbjct: 243  ADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNV-QSEHPYTKMRLTLD 301

Query: 992  IDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 1126
            +DMM+ +GG+ERTE EW+ L+  AG+  H+I  I AVQSVI AYP
Sbjct: 302  LDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346