BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00013947
         (2706 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   310   e-134
ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   278   e-121
ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|2...   268   e-119
ref|XP_004136614.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   248   e-110
ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabid...   252   e-108

>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
            gi|297739656|emb|CBI29838.3| unnamed protein product
            [Vitis vinifera]
          Length = 468

 Score =  310 bits (794), Expect(2) = e-134
 Identities = 160/242 (66%), Positives = 185/242 (76%), Gaps = 4/242 (1%)
 Frame = -3

Query: 1348 ALENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPVVLPLRDCVSQE 1169
            A EN Y KL+LGSCTSRIACHV+SATVKGQGYSLPADIQY DARWEIPVVLPLRDC++QE
Sbjct: 226  AYENGYTKLLLGSCTSRIACHVISATVKGQGYSLPADIQYVDARWEIPVVLPLRDCLAQE 285

Query: 1168 LNMLCRLNSLKTLDSFDSPRSGINELVSTFITLLMEENPARERTIVRTAEKLVPFQFNRI 989
            LNMLCRL+ LKT++  +SP SGIN LV++F+TLL EENP+RE TIVRTA KL PF FN+I
Sbjct: 286  LNMLCRLDGLKTMEVLNSPHSGINGLVASFVTLLQEENPSRECTIVRTAGKLTPFHFNQI 345

Query: 988  NESLDS----GDRRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLKSKLESCATSPT 821
             ES DS      RRR++K NLK  ES+  E  CPIC SP++  D   L S L S  T+  
Sbjct: 346  PESDDSNVHLASRRRQKKFNLKPNESVPPESYCPICNSPLSKSDHINL-SSLGSDQTNAD 404

Query: 820  VFGESCCPSCQFQILPKESSSMEHFYSLVPQQMITRVKDSVGGDQAWLREQIEDCLLSDG 641
            +FG +CCPSCQFQILPKE  SMEHFYSL+PQQ + + KD     Q  LREQI+D LLSD 
Sbjct: 405  IFGATCCPSCQFQILPKEPVSMEHFYSLLPQQFVVQAKDRSFSMQRQLREQIQDFLLSDS 464

Query: 640  ED 635
            ED
Sbjct: 465  ED 466



 Score =  198 bits (503), Expect(2) = e-134
 Identities = 114/221 (51%), Positives = 147/221 (66%), Gaps = 28/221 (12%)
 Frame = -1

Query: 2001 AGACGSGCYKAXXXXXXXXXEN-TGRT---------------CYKCKVAKIIESTGGL-- 1876
            AG C SGC++          +  T RT               C KCKV + + S+G +  
Sbjct: 5    AGNCQSGCFRDDDDSVVTEKKKLTNRTLSSGSDIDLPNHHNLCLKCKVNETLASSGTVSV 64

Query: 1875 -------CGECLDRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCSRVALEFVHEMQNKA 1717
                   C +C   +LF KFK AV++  +ISPTDNVL+AFSGGP SRVAL+FVHEMQ+KA
Sbjct: 65   DGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSGGPSSRVALQFVHEMQHKA 124

Query: 1716 QKNRDASRNKSYPVFGVGVAFIDETAVTSL---QLDKAIQDLRMIVSSLAPPRKELHIMP 1546
            QKN DASR++S PVFGVGVAF+DE+AV+ +   + DKAIQD++ IVS+LAPP K  H++P
Sbjct: 125  QKNFDASRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDMKSIVSNLAPPDKAFHVVP 184

Query: 1545 IQTILSDDDDVNRLKELLLTVTDVTGKEDLLLHLRMLCLQK 1423
            I+++ S +   +RL+ELL  V DVTGKEDLL HLRML LQK
Sbjct: 185  IESVYSSNGR-DRLEELLNAVKDVTGKEDLLQHLRMLSLQK 224


>ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Glycine max]
          Length = 458

 Score =  278 bits (711), Expect(2) = e-121
 Identities = 144/244 (59%), Positives = 180/244 (73%), Gaps = 4/244 (1%)
 Frame = -3

Query: 1351 VALENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPVVLPLRDCVSQ 1172
            VA E  YN++ILGSC SRIACHV+SATVKGQGYSLPADIQY DARWE+PVVLPLRDC +Q
Sbjct: 216  VASEFGYNRIILGSCISRIACHVISATVKGQGYSLPADIQYVDARWEVPVVLPLRDCFAQ 275

Query: 1171 ELNMLCRLNSLKTLDSFDSPRSGINELVSTFITLLMEENPARERTIVRTAEKLVPFQFNR 992
            E+NMLC L+ LKT+       S IN LVS+F+ LL EENP+RE TIVRTA KL PFQFNR
Sbjct: 276  EINMLCHLDGLKTVKLSTDLCSSINGLVSSFVALLQEENPSRESTIVRTAGKLTPFQFNR 335

Query: 991  INESLDS----GDRRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLKSKLESCATSP 824
            I E +D       RRR+++ NLKS ES+SSE  CP+C SP++  ++    S ++S  +S 
Sbjct: 336  IPEIIDGNVPLATRRRQKRYNLKSNESVSSESFCPLCNSPLDKSEITDW-SNIDSHRSS- 393

Query: 823  TVFGESCCPSCQFQILPKESSSMEHFYSLVPQQMITRVKDSVGGDQAWLREQIEDCLLSD 644
             +F  +CC SC+FQILP +S +ME FY  +PQ ++ R K +  G+ + LREQI+D LLSD
Sbjct: 394  DIFYNTCCSSCRFQILPSDSMAMEQFYMDLPQSVVGRAKQANNGNLSLLREQIQDYLLSD 453

Query: 643  GEDE 632
            GEDE
Sbjct: 454  GEDE 457



 Score =  187 bits (474), Expect(2) = e-121
 Identities = 102/211 (48%), Positives = 139/211 (65%), Gaps = 17/211 (8%)
 Frame = -1

Query: 2001 AGACGSGCYK-------AXXXXXXXXXENTGRTCYKCKVAKIIESTGGL-----CGECLD 1858
            +G    GCYK                 +N    C KCK+   +   GG+     C +C  
Sbjct: 6    SGCQSGGCYKDEDATCKQPPKSEEADSKNPSNVCIKCKLNDAVSGYGGIDDGRFCADCFK 65

Query: 1857 RSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCSRVALEFVHEMQNKAQKNRDASRNKSYP 1678
             +LF KF+ AV++  +I+PTD VL+AFSGGP SRVAL+FVH+MQ +AQ+N DASR++S P
Sbjct: 66   TNLFGKFRFAVTSNAMITPTDKVLVAFSGGPSSRVALQFVHDMQERAQRNFDASRDRSLP 125

Query: 1677 VFGVGVAFIDETAV---TSLQLDKAIQDLRMIVSSLAPPRKELHIMPIQTILSDD--DDV 1513
            VFGVGV FIDE+AV    S ++++A++ +  +VSSLAPPRKELHI+PI+T+ S D  D  
Sbjct: 126  VFGVGVVFIDESAVLPIPSNEMEEAVEVVSEVVSSLAPPRKELHIVPIETVYSSDSGDGK 185

Query: 1512 NRLKELLLTVTDVTGKEDLLLHLRMLCLQKV 1420
             R+ +++ TV D TG+ED+LL LRML LQKV
Sbjct: 186  ERITKVVNTVIDPTGREDVLLCLRMLALQKV 216


>ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|222854006|gb|EEE91553.1|
            predicted protein [Populus trichocarpa]
          Length = 443

 Score =  268 bits (684), Expect(2) = e-119
 Identities = 138/219 (63%), Positives = 169/219 (77%), Gaps = 4/219 (1%)
 Frame = -3

Query: 1351 VALENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPVVLPLRDCVSQ 1172
            VA +N YN+LILGSCTSRIACHVL+ATVKGQGYSL ADIQY DARWEIPVVLPLRDC SQ
Sbjct: 217  VASQNGYNRLILGSCTSRIACHVLAATVKGQGYSLSADIQYVDARWEIPVVLPLRDCTSQ 276

Query: 1171 ELNMLCRLNSLKTLDSFDSPRSGINELVSTFITLLMEENPARERTIVRTAEKLVPFQFNR 992
            ELN+LC+L+ LKTL  FDS  SGIN L+S+F+ +L EEN +RE TI+RTA KL PF+FNR
Sbjct: 277  ELNLLCQLDGLKTLQPFDSTPSGINGLISSFVNVLQEENSSRECTILRTAGKLTPFEFNR 336

Query: 991  INE----SLDSGDRRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLKSKLESCATSP 824
            I E    ++    RRR+++ NLK+ ESISSE  CPIC +P+ N +   L S L+SC +S 
Sbjct: 337  IPETDNCNVPLAIRRRQKRYNLKTKESISSELFCPICNAPLKNFNSLNL-SSLKSCQSSR 395

Query: 823  TVFGESCCPSCQFQILPKESSSMEHFYSLVPQQMITRVK 707
              F  +CC SCQFQI+PK+ SSM +F SL+PQQ++ R K
Sbjct: 396  --FNAACCSSCQFQIIPKDPSSMGNFCSLLPQQVVDRAK 432



 Score =  189 bits (479), Expect(2) = e-119
 Identities = 107/213 (50%), Positives = 140/213 (65%), Gaps = 19/213 (8%)
 Frame = -1

Query: 2001 AGACGSGCYK--------AXXXXXXXXXENTGRTCYKCKVAKIIE--STGG----LCGEC 1864
            +  C S CYK                   N+   C KCK    I   S  G     C +C
Sbjct: 5    SSGCQSNCYKNGDQVSDSPPPPPTLAAANNSNNLCLKCKTNSPITPGSLSGDEARFCADC 64

Query: 1863 LDRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCSRVALEFVHEMQNKAQKNRDASRNKS 1684
               +++ KF+ AV++Q +ISP+DNVL+AFSGGP SRVAL+FVH +Q++AQKN +AS++KS
Sbjct: 65   FRSNIYGKFRLAVTSQAMISPSDNVLVAFSGGPSSRVALQFVHGLQDRAQKNFEASKDKS 124

Query: 1683 YPVFGVGVAFIDETA---VTSLQLDKAIQDLRMIVSSLAPPRKELHIMPIQTILSDD--D 1519
             PVFGVGVAFIDE+A   V+S ++D AIQD+R +VS+LAPP K+LH++PI+ I + D  D
Sbjct: 125  LPVFGVGVAFIDESAVYPVSSDKVDGAIQDMRSVVSNLAPPTKQLHVVPIENIYASDSSD 184

Query: 1518 DVNRLKELLLTVTDVTGKEDLLLHLRMLCLQKV 1420
              +RL +LL  V D TGKEDLLLHLRML LQ V
Sbjct: 185  GKDRLTKLLDAVNDATGKEDLLLHLRMLALQMV 217


>ref|XP_004136614.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Cucumis
            sativus]
          Length = 469

 Score =  248 bits (632), Expect(2) = e-110
 Identities = 130/245 (53%), Positives = 172/245 (70%), Gaps = 5/245 (2%)
 Frame = -3

Query: 1351 VALENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPVVLPLRDCVSQ 1172
            +A EN YN+L++G+CTSRIACHV+S+TVKGQGYSLP D+QY DARWE+PVVLPL DC++Q
Sbjct: 232  IAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQ 291

Query: 1171 ELNMLCRLNSLKTLDSFDSPRSGINELVSTFITLLMEENPARERTIVRTAEKLVPFQFNR 992
            ELNMLC ++ LKT++      SGIN+LVS+F+ LL  ENP+RE TIVRTA KL PF F+R
Sbjct: 292  ELNMLCCVDGLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSR 351

Query: 991  INESLDSGD-----RRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLKSKLESCATS 827
            I +  +S +     +RR+++ N K   S+SSE  C +C  P+N  D+      L   A S
Sbjct: 352  IPDLNESNNVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDV------LTRHAGS 405

Query: 826  PTVFGESCCPSCQFQILPKESSSMEHFYSLVPQQMITRVKDSVGGDQAWLREQIEDCLLS 647
             ++ G +CC SCQFQILPK+SSS E F SL+P  M+ R      G+   LR++I++ LLS
Sbjct: 406  -SILG-ACCSSCQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLS 463

Query: 646  DGEDE 632
            D E E
Sbjct: 464  DDEAE 468



 Score =  180 bits (457), Expect(2) = e-110
 Identities = 94/178 (52%), Positives = 130/178 (73%), Gaps = 10/178 (5%)
 Frame = -1

Query: 1923 CYKCKVAKIIESTG-----GLCGECLDRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCS 1759
            C+KCK  + + ++G       C +C   +LFAKF+QAV++  +ISP+D VL+AFSGGPCS
Sbjct: 55   CFKCKSKERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCS 114

Query: 1758 RVALEFVHEMQNKAQKNRDASRNKSYPVFGVGVAFIDETAVTSL---QLDKAIQDLRMIV 1588
            RVAL+FVHEMQ KA  N +ASR++S  VFGVGVA+I+ETA  S+   ++DK +Q ++ +V
Sbjct: 115  RVALQFVHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVV 174

Query: 1587 SSLAPPRKELHIMPIQTILSD--DDDVNRLKELLLTVTDVTGKEDLLLHLRMLCLQKV 1420
            SSL  PRK LHI+ I+++ S   +D   +LK+LL  V DVTGKEDL+ +LRML LQK+
Sbjct: 175  SSLCQPRKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKI 232


>ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabidopsis thaliana]
            gi|332278212|sp|O65628.3|CTU2_ARATH RecName:
            Full=Cytoplasmic tRNA 2-thiolation protein 2
            gi|115646788|gb|ABJ17118.1| At4g35910 [Arabidopsis
            thaliana] gi|332661188|gb|AEE86588.1| cytoplasmic tRNA
            2-thiolation protein 2 [Arabidopsis thaliana]
          Length = 458

 Score =  252 bits (643), Expect(2) = e-108
 Identities = 132/245 (53%), Positives = 171/245 (69%), Gaps = 4/245 (1%)
 Frame = -3

Query: 1351 VALENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPVVLPLRDCVSQ 1172
            VA EN YN+L+LGSCTSRIA HVL+ATVKG+GYSL ADIQ+ DARW++P+VLPLRDCV  
Sbjct: 216  VAAENGYNRLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRL 275

Query: 1171 ELNMLCRLNSLKTLDSFDSPRSGINELVSTFITLLMEENPARERTIVRTAEKLVPFQFNR 992
            E+  LC L+ LKT++     + GIN+LVS+F+ LL EENP+RE TIVRTA KL PF FN+
Sbjct: 276  EITRLCLLDGLKTVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNK 335

Query: 991  INESLDS----GDRRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLKSKLESCATSP 824
            I E+ DS      +RR ++ NLK   S+++E  CPIC  P+N  D   L +  E      
Sbjct: 336  IPETDDSNVPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEE--GQES 393

Query: 823  TVFGESCCPSCQFQILPKESSSMEHFYSLVPQQMITRVKDSVGGDQAWLREQIEDCLLSD 644
             V   +CC SC+FQILP++ SS+E F S +P  MI++VK      QA+LRE+I+DCLL D
Sbjct: 394  DVLYAACCSSCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLD 453

Query: 643  GEDEV 629
             E+ V
Sbjct: 454  DEEVV 458



 Score =  170 bits (430), Expect(2) = e-108
 Identities = 99/212 (46%), Positives = 133/212 (62%), Gaps = 18/212 (8%)
 Frame = -1

Query: 2001 AGACGSGCY-------KAXXXXXXXXXENTGRTCYKCKV-AKIIESTGGL-----CGECL 1861
            +  C SGCY                   N    C KCK  A +    GG      C +C 
Sbjct: 5    SSGCESGCYDREKDNGSKIVDDAVSGGGNHESVCVKCKCNAPMTFGDGGFDDGRFCADCF 64

Query: 1860 DRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCSRVALEFVHEMQNKAQKNRDASRNKSY 1681
              ++F KF+ AV++  +I+P+DNVL+AFSGG  SRV+L+FVHE+Q KA KN +ASR++S 
Sbjct: 65   RNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQIKALKNYEASRDRSL 124

Query: 1680 PVFGVGVAFIDETA---VTSLQLDKAIQDLRMIVSSLAPPRKELHIMPIQTILSDD--DD 1516
            PVFGVGVAF+DETA     S ++  AI+ +R  VS L+PP K+LH++P+++I   D  D 
Sbjct: 125  PVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKDLHVVPVESIFGSDSLDA 184

Query: 1515 VNRLKELLLTVTDVTGKEDLLLHLRMLCLQKV 1420
             +RL +LL +V D TGKEDLLLHL+ML LQKV
Sbjct: 185  RDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKV 216