BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00013733
         (1630 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI24934.3| unnamed protein product [Vitis vinifera]              626   e-177
ref|XP_002276734.1| PREDICTED: cycloartenol-C-24-methyltransfera...   624   e-176
gb|AAZ83345.1| 24-sterol C-methyltransferase [Gossypium hirsutum]     619   e-175
ref|XP_004149721.1| PREDICTED: cycloartenol-C-24-methyltransfera...   613   e-173
ref|XP_003599380.1| Cycloartenol-C-24-methyltransferase [Medicag...   603   e-170

>emb|CBI24934.3| unnamed protein product [Vitis vinifera]
          Length = 421

 Score =  626 bits (1615), Expect = e-177
 Identities = 301/346 (86%), Positives = 320/346 (92%)
 Frame = -1

Query: 1342 FGKMSKGGAFDLASGVGGQINKKEVLSAVDTYEKYHVSYGGEEEDRKANYTDMVNKYYDL 1163
            F +MSK GA DLASGVGG+I K EVLSAV+ YEKYHV YGGEEE+RKANY+DMVNKYYDL
Sbjct: 66   FAEMSKAGALDLASGVGGKIQKDEVLSAVEKYEKYHVCYGGEEEERKANYSDMVNKYYDL 125

Query: 1162 STSFYEFGWGESFHFANRWNGESLRESIKRHEHYLALQLCLKPGQKVLDVGCGIGGPLRE 983
             TSFYEFGWGESFHFA RW GESLRESI+RHEH+LALQL +KPGQKVLDVGCGIGGPLRE
Sbjct: 126  VTSFYEFGWGESFHFAPRWKGESLRESIRRHEHFLALQLGVKPGQKVLDVGCGIGGPLRE 185

Query: 982  IARFSSTSVTGLNNNEYQISRGKELNRVAGVDKTCKFVKADFMKMPFEENTFDAVYAIEA 803
            IARFSSTSVTGLNNNEYQI+RG+ELN +AGVDKTC FVKADFMKMPF +NTFDAVYAIEA
Sbjct: 186  IARFSSTSVTGLNNNEYQITRGRELNCIAGVDKTCDFVKADFMKMPFSDNTFDAVYAIEA 245

Query: 802  TCHAPDALGCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQRIKAEIELGDGLPDIRL 623
            TCHAPDALGCYKEIYRVLKPGQCFAAYEWCMTD+FDPNNQEHQ+IKAEIE+GDGLPDIRL
Sbjct: 246  TCHAPDALGCYKEIYRVLKPGQCFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRL 305

Query: 622  TKQCLEALKLAGFDVIWEKDLAEDSPLPWYLPLDTSRLSFSSFRLTTVGRFITTNMVKAL 443
            T+QCLEALK AGF+VIWEKDLA  SPLPWYLPLD S  S SSFRLT VGRFIT NMVKAL
Sbjct: 306  TRQCLEALKQAGFEVIWEKDLAVGSPLPWYLPLDKSHFSLSSFRLTAVGRFITKNMVKAL 365

Query: 442  EFVGLAPKGSQRVQSFLEKAAESLVSGGRKEIFTPMYFFLARKPLS 305
            EFVGLAPKGSQRVQ+FLEKAAE LV GG+KEIFTPMYFFLARKP S
Sbjct: 366  EFVGLAPKGSQRVQAFLEKAAEGLVDGGKKEIFTPMYFFLARKPNS 411


>ref|XP_002276734.1| PREDICTED: cycloartenol-C-24-methyltransferase isoform 1 [Vitis
            vinifera] gi|359487307|ref|XP_003633564.1| PREDICTED:
            cycloartenol-C-24-methyltransferase isoform 2 [Vitis
            vinifera]
          Length = 353

 Score =  624 bits (1608), Expect = e-176
 Identities = 300/343 (87%), Positives = 318/343 (92%)
 Frame = -1

Query: 1333 MSKGGAFDLASGVGGQINKKEVLSAVDTYEKYHVSYGGEEEDRKANYTDMVNKYYDLSTS 1154
            MSK GA DLASGVGG+I K EVLSAV+ YEKYHV YGGEEE+RKANY+DMVNKYYDL TS
Sbjct: 1    MSKAGALDLASGVGGKIQKDEVLSAVEKYEKYHVCYGGEEEERKANYSDMVNKYYDLVTS 60

Query: 1153 FYEFGWGESFHFANRWNGESLRESIKRHEHYLALQLCLKPGQKVLDVGCGIGGPLREIAR 974
            FYEFGWGESFHFA RW GESLRESI+RHEH+LALQL +KPGQKVLDVGCGIGGPLREIAR
Sbjct: 61   FYEFGWGESFHFAPRWKGESLRESIRRHEHFLALQLGVKPGQKVLDVGCGIGGPLREIAR 120

Query: 973  FSSTSVTGLNNNEYQISRGKELNRVAGVDKTCKFVKADFMKMPFEENTFDAVYAIEATCH 794
            FSSTSVTGLNNNEYQI+RG+ELN +AGVDKTC FVKADFMKMPF +NTFDAVYAIEATCH
Sbjct: 121  FSSTSVTGLNNNEYQITRGRELNCIAGVDKTCDFVKADFMKMPFSDNTFDAVYAIEATCH 180

Query: 793  APDALGCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQRIKAEIELGDGLPDIRLTKQ 614
            APDALGCYKEIYRVLKPGQCFAAYEWCMTD+FDPNNQEHQ+IKAEIE+GDGLPDIRLT+Q
Sbjct: 181  APDALGCYKEIYRVLKPGQCFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRLTRQ 240

Query: 613  CLEALKLAGFDVIWEKDLAEDSPLPWYLPLDTSRLSFSSFRLTTVGRFITTNMVKALEFV 434
            CLEALK AGF+VIWEKDLA  SPLPWYLPLD S  S SSFRLT VGRFIT NMVKALEFV
Sbjct: 241  CLEALKQAGFEVIWEKDLAVGSPLPWYLPLDKSHFSLSSFRLTAVGRFITKNMVKALEFV 300

Query: 433  GLAPKGSQRVQSFLEKAAESLVSGGRKEIFTPMYFFLARKPLS 305
            GLAPKGSQRVQ+FLEKAAE LV GG+KEIFTPMYFFLARKP S
Sbjct: 301  GLAPKGSQRVQAFLEKAAEGLVDGGKKEIFTPMYFFLARKPNS 343


>gb|AAZ83345.1| 24-sterol C-methyltransferase [Gossypium hirsutum]
          Length = 346

 Score =  619 bits (1596), Expect = e-175
 Identities = 298/343 (86%), Positives = 316/343 (92%)
 Frame = -1

Query: 1333 MSKGGAFDLASGVGGQINKKEVLSAVDTYEKYHVSYGGEEEDRKANYTDMVNKYYDLSTS 1154
            MSK GA DLASG+GG+I K EVLSAV+ YE+YHV YGGEEE+RKANYTDMVNKYYDL TS
Sbjct: 1    MSKAGALDLASGLGGKIEKAEVLSAVEQYERYHVFYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 1153 FYEFGWGESFHFANRWNGESLRESIKRHEHYLALQLCLKPGQKVLDVGCGIGGPLREIAR 974
            FYEFGWGESFHFA RWNGESL+ESIKRHEH+LALQL LKPG KVLDVGCGIGGPLREIAR
Sbjct: 61   FYEFGWGESFHFAPRWNGESLKESIKRHEHFLALQLGLKPGHKVLDVGCGIGGPLREIAR 120

Query: 973  FSSTSVTGLNNNEYQISRGKELNRVAGVDKTCKFVKADFMKMPFEENTFDAVYAIEATCH 794
            FSSTSVTG+NNNEYQI+RGKELNR+AGV+KTC FVKADFMKMPF ++ FDAVYAIEATCH
Sbjct: 121  FSSTSVTGINNNEYQITRGKELNRIAGVEKTCNFVKADFMKMPFPDSAFDAVYAIEATCH 180

Query: 793  APDALGCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQRIKAEIELGDGLPDIRLTKQ 614
            APDA GCYKEIYRVLKPGQ FAAYEWCMTDSFDPNN EHQ+IKAEIE+GDGLPDIRLT Q
Sbjct: 181  APDAYGCYKEIYRVLKPGQYFAAYEWCMTDSFDPNNSEHQKIKAEIEIGDGLPDIRLTGQ 240

Query: 613  CLEALKLAGFDVIWEKDLAEDSPLPWYLPLDTSRLSFSSFRLTTVGRFITTNMVKALEFV 434
            CLEALK AGF++IWEKDLA  SPLPWYLPLDTS  S SSFRLT+VGRFIT NMVKALEFV
Sbjct: 241  CLEALKEAGFEIIWEKDLAVGSPLPWYLPLDTSHFSLSSFRLTSVGRFITRNMVKALEFV 300

Query: 433  GLAPKGSQRVQSFLEKAAESLVSGGRKEIFTPMYFFLARKPLS 305
            GLAPKGSQRVQ FLEKAAE LV GGRKEIFTPMYFFLARKPLS
Sbjct: 301  GLAPKGSQRVQDFLEKAAEGLVEGGRKEIFTPMYFFLARKPLS 343


>ref|XP_004149721.1| PREDICTED: cycloartenol-C-24-methyltransferase-like [Cucumis sativus]
            gi|449517755|ref|XP_004165910.1| PREDICTED:
            cycloartenol-C-24-methyltransferase-like [Cucumis
            sativus]
          Length = 346

 Score =  613 bits (1580), Expect = e-173
 Identities = 296/345 (85%), Positives = 315/345 (91%)
 Frame = -1

Query: 1333 MSKGGAFDLASGVGGQINKKEVLSAVDTYEKYHVSYGGEEEDRKANYTDMVNKYYDLSTS 1154
            MSK GA DLASG+GG++ K EVLSAV+ YEKYHV YGGEEE+RKANYTDMVNKYYDL TS
Sbjct: 1    MSKTGALDLASGLGGKLEKNEVLSAVEKYEKYHVCYGGEEEERKANYTDMVNKYYDLVTS 60

Query: 1153 FYEFGWGESFHFANRWNGESLRESIKRHEHYLALQLCLKPGQKVLDVGCGIGGPLREIAR 974
            FYEFGWGESFHFA RW GESLRESIKRHEH+LALQL LKPG KVLDVGCGIGGPLREIAR
Sbjct: 61   FYEFGWGESFHFAPRWKGESLRESIKRHEHFLALQLDLKPGYKVLDVGCGIGGPLREIAR 120

Query: 973  FSSTSVTGLNNNEYQISRGKELNRVAGVDKTCKFVKADFMKMPFEENTFDAVYAIEATCH 794
            FS TSVTGLNNNEYQISRGKELNRVA VD+TC FVKADFMKMPF +N+FDAVYAIEATCH
Sbjct: 121  FSYTSVTGLNNNEYQISRGKELNRVAKVDRTCDFVKADFMKMPFPDNSFDAVYAIEATCH 180

Query: 793  APDALGCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQRIKAEIELGDGLPDIRLTKQ 614
            APDA GCYKEIYRVLKPGQ FAAYEWCMTD+FDPNNQEHQ+IKAEIE+GDGLPDIR+T +
Sbjct: 181  APDAYGCYKEIYRVLKPGQHFAAYEWCMTDAFDPNNQEHQKIKAEIEIGDGLPDIRMTGK 240

Query: 613  CLEALKLAGFDVIWEKDLAEDSPLPWYLPLDTSRLSFSSFRLTTVGRFITTNMVKALEFV 434
            CLEALK AGF+VIWEKDLAE+SPLPWYLPLD S  S SSFRLT +GRFIT NMVKALEF+
Sbjct: 241  CLEALKQAGFEVIWEKDLAENSPLPWYLPLDKSHFSLSSFRLTALGRFITKNMVKALEFI 300

Query: 433  GLAPKGSQRVQSFLEKAAESLVSGGRKEIFTPMYFFLARKPLSGT 299
             LAPKGSQRVQ FLEKAAE LV GG+KEIFTPMYFFLARKPLS T
Sbjct: 301  RLAPKGSQRVQDFLEKAAEGLVEGGKKEIFTPMYFFLARKPLSAT 345


>ref|XP_003599380.1| Cycloartenol-C-24-methyltransferase [Medicago truncatula]
            gi|217071918|gb|ACJ84319.1| unknown [Medicago truncatula]
            gi|355488428|gb|AES69631.1|
            Cycloartenol-C-24-methyltransferase [Medicago truncatula]
          Length = 340

 Score =  603 bits (1556), Expect = e-170
 Identities = 288/336 (85%), Positives = 307/336 (91%)
 Frame = -1

Query: 1312 DLASGVGGQINKKEVLSAVDTYEKYHVSYGGEEEDRKANYTDMVNKYYDLSTSFYEFGWG 1133
            DLAS +GG+I K +VLSAVD YEKYH SYGG+E++RKANY+DMVNKYYDL+TSFYEFGWG
Sbjct: 2    DLASNLGGKIEKAQVLSAVDKYEKYHASYGGQEDERKANYSDMVNKYYDLATSFYEFGWG 61

Query: 1132 ESFHFANRWNGESLRESIKRHEHYLALQLCLKPGQKVLDVGCGIGGPLREIARFSSTSVT 953
            ESFHFA RW GESLRESIKRHEH+LALQL L PGQKVLDVGCGIGGPLREI+RFSSTSVT
Sbjct: 62   ESFHFATRWVGESLRESIKRHEHFLALQLGLTPGQKVLDVGCGIGGPLREISRFSSTSVT 121

Query: 952  GLNNNEYQISRGKELNRVAGVDKTCKFVKADFMKMPFEENTFDAVYAIEATCHAPDALGC 773
            GLNNNEYQI+RGK LNR  GVDKTC FVKADFMKMPFE+N+FDAVYAIEATCHAPDA GC
Sbjct: 122  GLNNNEYQITRGKALNRKTGVDKTCNFVKADFMKMPFEDNSFDAVYAIEATCHAPDAYGC 181

Query: 772  YKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQRIKAEIELGDGLPDIRLTKQCLEALKL 593
            YKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQ+IKAEIE+GDGLPDIRLT +CLEALK 
Sbjct: 182  YKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDIRLTTKCLEALKQ 241

Query: 592  AGFDVIWEKDLAEDSPLPWYLPLDTSRLSFSSFRLTTVGRFITTNMVKALEFVGLAPKGS 413
            AGF+VIWEKDLA DSPLPWYLPLD S  S SSFRLT VGR  T NMVKALEFVGLAPKGS
Sbjct: 242  AGFEVIWEKDLASDSPLPWYLPLDKSHFSLSSFRLTAVGRLFTRNMVKALEFVGLAPKGS 301

Query: 412  QRVQSFLEKAAESLVSGGRKEIFTPMYFFLARKPLS 305
            QRVQ FLEKAAE LV GG++EIFTPMYFFLARKP S
Sbjct: 302  QRVQDFLEKAAEGLVEGGKREIFTPMYFFLARKPAS 337