BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00013541
         (1149 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277376.1| PREDICTED: uncharacterized protein LOC100262...   256   7e-66
emb|CBI33855.3| unnamed protein product [Vitis vinifera]              256   7e-66
ref|XP_004136196.1| PREDICTED: E3 ubiquitin-protein ligase RNF14...   253   5e-65
ref|XP_002326535.1| predicted protein [Populus trichocarpa] gi|2...   253   8e-65
ref|NP_174512.1| ubiquitin-protein ligase RNF14 [Arabidopsis tha...   241   2e-61

>ref|XP_002277376.1| PREDICTED: uncharacterized protein LOC100262589 [Vitis vinifera]
          Length = 868

 Score =  256 bits (654), Expect = 7e-66
 Identities = 127/252 (50%), Positives = 161/252 (63%), Gaps = 27/252 (10%)
 Frame = -3

Query: 1147 LKRLHARDDFEHWESLLLQKTLDSMSDVVYCPRCETACLEDGDGHAQCLKCFFSFCILCS 968
            LKRL   ++FEHWESL+LQKTL+SMSD+ YCPRCET C+ED D HAQC KCFFSFC LC 
Sbjct: 618  LKRLLGDEEFEHWESLMLQKTLESMSDMTYCPRCETICIEDEDQHAQCSKCFFSFCTLCR 677

Query: 967  EQRHVGTTCMGPEKRLQTLMELKELRDLKEEQSYEIQELNNEILSLKIIFCDAKQCPSCN 788
            ++RHVG  CM PE +L  L   +    LK+EQ  + +E+ NE+LS+K I  DAKQCPSC 
Sbjct: 678  DRRHVGLECMTPEVKLHILQGRQHSSQLKDEQRKKEREMINELLSVKEILRDAKQCPSCK 737

Query: 787  IAISRIEGCNRMQCRNCGQVFCWGCNKALNGNSH---PFCE--------------SRQRN 659
            +AISR EGCN+M C NCGQ FC+ CN+A++G  H     CE                + N
Sbjct: 738  MAISRTEGCNKMVCNNCGQYFCYRCNQAIDGYEHFRDSACELFPQEMIQNWEWDWEARLN 797

Query: 658  KKLVV----------KKHCCPFCGEKNAKIQVDDNNHMVCLACQKHYCYLCRKSFEESSQ 509
            ++ VV          + H CP C + N K  V +NNH+ C +CQ HYCYLC++    SSQ
Sbjct: 798  QRQVVGQIQAELFADRGHSCPICRQINVK--VGNNNHIFCWSCQSHYCYLCKEIVRRSSQ 855

Query: 508  HYTLKDCRQQHT 473
            H+  K C+ QHT
Sbjct: 856  HFGPKGCK-QHT 866


>emb|CBI33855.3| unnamed protein product [Vitis vinifera]
          Length = 662

 Score =  256 bits (654), Expect = 7e-66
 Identities = 127/252 (50%), Positives = 161/252 (63%), Gaps = 27/252 (10%)
 Frame = -3

Query: 1147 LKRLHARDDFEHWESLLLQKTLDSMSDVVYCPRCETACLEDGDGHAQCLKCFFSFCILCS 968
            LKRL   ++FEHWESL+LQKTL+SMSD+ YCPRCET C+ED D HAQC KCFFSFC LC 
Sbjct: 412  LKRLLGDEEFEHWESLMLQKTLESMSDMTYCPRCETICIEDEDQHAQCSKCFFSFCTLCR 471

Query: 967  EQRHVGTTCMGPEKRLQTLMELKELRDLKEEQSYEIQELNNEILSLKIIFCDAKQCPSCN 788
            ++RHVG  CM PE +L  L   +    LK+EQ  + +E+ NE+LS+K I  DAKQCPSC 
Sbjct: 472  DRRHVGLECMTPEVKLHILQGRQHSSQLKDEQRKKEREMINELLSVKEILRDAKQCPSCK 531

Query: 787  IAISRIEGCNRMQCRNCGQVFCWGCNKALNGNSH---PFCE--------------SRQRN 659
            +AISR EGCN+M C NCGQ FC+ CN+A++G  H     CE                + N
Sbjct: 532  MAISRTEGCNKMVCNNCGQYFCYRCNQAIDGYEHFRDSACELFPQEMIQNWEWDWEARLN 591

Query: 658  KKLVV----------KKHCCPFCGEKNAKIQVDDNNHMVCLACQKHYCYLCRKSFEESSQ 509
            ++ VV          + H CP C + N K  V +NNH+ C +CQ HYCYLC++    SSQ
Sbjct: 592  QRQVVGQIQAELFADRGHSCPICRQINVK--VGNNNHIFCWSCQSHYCYLCKEIVRRSSQ 649

Query: 508  HYTLKDCRQQHT 473
            H+  K C+ QHT
Sbjct: 650  HFGPKGCK-QHT 660


>ref|XP_004136196.1| PREDICTED: E3 ubiquitin-protein ligase RNF14-like [Cucumis sativus]
            gi|449525814|ref|XP_004169911.1| PREDICTED: E3
            ubiquitin-protein ligase RNF14-like [Cucumis sativus]
          Length = 638

 Score =  253 bits (647), Expect = 5e-65
 Identities = 129/248 (52%), Positives = 162/248 (65%), Gaps = 23/248 (9%)
 Frame = -3

Query: 1147 LKRLHARDDFEHWESLLLQKTLDSMSDVVYCPRCETACLEDGDGHAQCLKCFFSFCILCS 968
            LK+L   ++FE WES++L KTL+SMSDVVYCPRCET CLED D  AQC KC+FSFC LCS
Sbjct: 392  LKQLLGDEEFERWESMMLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCS 451

Query: 967  EQRHVGTTCMGPEKRLQTLMELKELRDLKEEQSYEIQELNNEILSLKIIFCDAKQCPSCN 788
            E+RHVG  CM PE +L+ L E +    L  EQ  + +E+ NE++S+K I  DAKQCPSC 
Sbjct: 452  ERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDAKQCPSCK 511

Query: 787  IAISRIEGCNRMQCRNCGQVFCWGCNKALNGNSH---PFCE----------SRQRNKKLV 647
            +AISR EGCN+M C NCGQ FC+ C+KA++G  H     CE            + N + V
Sbjct: 512  MAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQEAIQQWEERMNARQV 571

Query: 646  VKK----------HCCPFCGEKNAKIQVDDNNHMVCLACQKHYCYLCRKSFEESSQHYTL 497
            + +          H CP CG+ NAK  V +NNH+ C +CQ HYCYLCRK  + SSQHY  
Sbjct: 572  LGQIQAELFPENGHPCPNCGQLNAK--VGNNNHIRCWSCQIHYCYLCRKVVKRSSQHYGP 629

Query: 496  KDCRQQHT 473
            K C+ QHT
Sbjct: 630  KGCK-QHT 636


>ref|XP_002326535.1| predicted protein [Populus trichocarpa] gi|222833857|gb|EEE72334.1|
            predicted protein [Populus trichocarpa]
          Length = 642

 Score =  253 bits (645), Expect = 8e-65
 Identities = 123/248 (49%), Positives = 158/248 (63%), Gaps = 23/248 (9%)
 Frame = -3

Query: 1147 LKRLHARDDFEHWESLLLQKTLDSMSDVVYCPRCETACLEDGDGHAQCLKCFFSFCILCS 968
            LKRL   +++E WESL+LQKTL+SMSDV YCPRCET C+ED + HAQC KC +SFC LC 
Sbjct: 396  LKRLLGDEEYERWESLMLQKTLESMSDVSYCPRCETPCIEDEEQHAQCSKCLYSFCTLCR 455

Query: 967  EQRHVGTTCMGPEKRLQTLMELKELRDLKEEQSYEIQELNNEILSLKIIFCDAKQCPSCN 788
            E+RH+G  CM PE +LQ L E +    LK+ Q +  +E+ NE+LS+K I  DAKQCPSC 
Sbjct: 456  ERRHLGEVCMTPEMKLQVLEERQNSSHLKDGQKHREREMINELLSVKEILRDAKQCPSCK 515

Query: 787  IAISRIEGCNRMQCRNCGQVFCWGCNKALNGNSH--------------PFCESRQRNKKL 650
            +AISR EGCN+M C+NC Q FC+ CNK ++G  H                 E R   +++
Sbjct: 516  MAISRTEGCNKMVCKNCEQFFCYRCNKGIDGYDHFKDGQCELFPQEEIQQWEERMNPRRV 575

Query: 649  VVKKH---------CCPFCGEKNAKIQVDDNNHMVCLACQKHYCYLCRKSFEESSQHYTL 497
            V + H          CP CG+ N K  V +NNH++C +CQ HYCYLCRK     SQH+  
Sbjct: 576  VAEVHAELFPDHVNACPNCGQFNVK--VGNNNHILCWSCQMHYCYLCRKIVRRGSQHFGP 633

Query: 496  KDCRQQHT 473
            K C+ QHT
Sbjct: 634  KGCK-QHT 640


>ref|NP_174512.1| ubiquitin-protein ligase RNF14 [Arabidopsis thaliana]
            gi|8920608|gb|AAF81330.1|AC007767_10 Contains similarity
            to ring finger protein 14 from Homo sapiens gb|NM_004290.
            It contains an IBR domain PF|01485 [Arabidopsis thaliana]
            gi|12597865|gb|AAG60174.1|AC084110_7 hypothetical protein
            [Arabidopsis thaliana] gi|20466165|gb|AAM20400.1| RING
            finger protein, putative [Arabidopsis thaliana]
            gi|25083846|gb|AAN72126.1| RING finger protein, putative
            [Arabidopsis thaliana] gi|332193345|gb|AEE31466.1|
            ubiquitin-protein ligase RNF14 [Arabidopsis thaliana]
          Length = 688

 Score =  241 bits (615), Expect = 2e-61
 Identities = 121/255 (47%), Positives = 157/255 (61%), Gaps = 30/255 (11%)
 Frame = -3

Query: 1147 LKRLHARDDFEHWESLLLQKTLDSMSDVVYCPRCETACLEDGDGHAQCLKCFFSFCILCS 968
            LKRL   + +E WE+L+LQKTL+SM+DV YCPRCET C+ED +  A C KC+FSFC LC 
Sbjct: 436  LKRLLGDEAYERWETLMLQKTLESMTDVAYCPRCETPCIEDEEQLALCFKCYFSFCTLCK 495

Query: 967  EQRHVGTTCMGPEKRLQTLMELKELRDLKEEQSYEIQELNNEILSLKIIFCDAKQCPSCN 788
            E+RHVG  CM PE RLQ L E ++   L EEQ  + +E+ NEI+S+K+I   AKQCPSC 
Sbjct: 496  EKRHVGVACMSPELRLQILQERQDSSRLGEEQRRKEKEMINEIMSVKVIMKSAKQCPSCK 555

Query: 787  IAISRIEGCNRMQCRNCGQVFCWGCNKALNGNSHPFCESR--------------QRNKKL 650
            IAISR  GCN+M C NCGQ FC+ CN+A+ G  H F E +                N++ 
Sbjct: 556  IAISRTGGCNKMVCNNCGQYFCYRCNEAITGYEH-FSEGKCELFPQEAIQEWNEMMNERQ 614

Query: 649  VV----------------KKHCCPFCGEKNAKIQVDDNNHMVCLACQKHYCYLCRKSFEE 518
            V+                +   CP C + N K    +NNH+ C ACQ H+CYLC+K  ++
Sbjct: 615  VIGQIQAQLFAQGGQFPQRGQLCPNCRQFNGK--AGNNNHLFCWACQAHFCYLCKKVVKK 672

Query: 517  SSQHYTLKDCRQQHT 473
            S+QHY  K C+ QHT
Sbjct: 673  SAQHYGPKGCK-QHT 686