BLASTX nr result
ID: Coptis25_contig00013421
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00013421
(1037 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002513320.1| 7-dehydrocholesterol reductase, putative [Ri... 145 3e-50
ref|XP_004159005.1| PREDICTED: LOW QUALITY PROTEIN: 7-dehydrocho... 144 2e-49
ref|XP_004141748.1| PREDICTED: 7-dehydrocholesterol reductase-li... 144 2e-49
ref|XP_002268455.2| PREDICTED: 7-dehydrocholesterol reductase-li... 142 2e-49
emb|CBI18435.3| unnamed protein product [Vitis vinifera] 142 2e-49
>ref|XP_002513320.1| 7-dehydrocholesterol reductase, putative [Ricinus communis]
gi|223547228|gb|EEF48723.1| 7-dehydrocholesterol
reductase, putative [Ricinus communis]
Length = 560
Score = 145 bits (365), Expect(2) = 3e-50
Identities = 65/79 (82%), Positives = 72/79 (91%)
Frame = -2
Query: 358 IYVN*DCDRQRQEFRKKNGKCLIWDRFPSKIVASYQTGNGETKTSLLLTSGWWGLARHFH 179
I+VN DCDRQRQEFR+KNGKCL+W + PSKIVASY T +GETKTSLLLTSGWWGL+RHFH
Sbjct: 307 IFVNYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWWGLSRHFH 366
Query: 178 YVPEILSAFFWTVTALFIH 122
YVPEIL+AFFWTV ALF H
Sbjct: 367 YVPEILAAFFWTVPALFNH 385
Score = 80.9 bits (198), Expect(2) = 3e-50
Identities = 36/46 (78%), Positives = 40/46 (86%), Gaps = 4/46 (8%)
Frame = -1
Query: 485 AGFYICWGCLVWVPSIYPSPGMYLV----NLGTQLALFILAAGLLC 360
AGFYICWGCLVWVPS+Y SPGMYLV NLGTQLA++IL AG+LC
Sbjct: 261 AGFYICWGCLVWVPSVYTSPGMYLVNHPLNLGTQLAVYILVAGILC 306
Score = 109 bits (273), Expect(2) = 4e-37
Identities = 55/81 (67%), Positives = 64/81 (79%), Gaps = 3/81 (3%)
Frame = -1
Query: 1037 CIFLYIKGHLAPSSTDSGSSGNAIIDFY*GMELYPRIGKNFDVKVFTNCRFGMMS---LG 867
C++LYIKGHLAPSSTDSGS GN I+DFY GMELYPRIGKNFD+KVFTNCRFGMMS L
Sbjct: 152 CVYLYIKGHLAPSSTDSGSCGNIIMDFYWGMELYPRIGKNFDIKVFTNCRFGMMSWAVLA 211
Query: 866 SSCCDISYQAETGQVATLCLL 804
+ C Y+ + G+VA L+
Sbjct: 212 LTYCIKQYE-QNGKVADSMLV 231
Score = 72.4 bits (176), Expect(2) = 4e-37
Identities = 30/35 (85%), Positives = 33/35 (94%)
Frame = -3
Query: 822 SDSMLVNMALMVMYVTKFFWWEAGYWNTMDIAHDR 718
+DSMLVN LM++YVTKFFWWEAGYWNTMDIAHDR
Sbjct: 226 ADSMLVNTILMLVYVTKFFWWEAGYWNTMDIAHDR 260
>ref|XP_004159005.1| PREDICTED: LOW QUALITY PROTEIN: 7-dehydrocholesterol reductase-like
[Cucumis sativus]
Length = 435
Score = 144 bits (364), Expect(2) = 2e-49
Identities = 64/79 (81%), Positives = 71/79 (89%)
Frame = -2
Query: 358 IYVN*DCDRQRQEFRKKNGKCLIWDRFPSKIVASYQTGNGETKTSLLLTSGWWGLARHFH 179
IY+N DCDRQRQEFR+KNGKCL+W + PSKIVA+Y T GET+TSLLLTSGWWGLARHFH
Sbjct: 308 IYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVATYTTTTGETRTSLLLTSGWWGLARHFH 367
Query: 178 YVPEILSAFFWTVTALFIH 122
YVPEIL+AFFWTV ALF H
Sbjct: 368 YVPEILAAFFWTVPALFHH 386
Score = 79.0 bits (193), Expect(2) = 2e-49
Identities = 36/46 (78%), Positives = 39/46 (84%), Gaps = 4/46 (8%)
Frame = -1
Query: 485 AGFYICWGCLVWVPSIYPSPGMYL----VNLGTQLALFILAAGLLC 360
AGFYICWGCLVWVPS+Y SPGMYL VNLGTQLA+ IL AG+LC
Sbjct: 262 AGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSILVAGILC 307
Score = 105 bits (261), Expect(3) = 7e-38
Identities = 47/55 (85%), Positives = 52/55 (94%)
Frame = -1
Query: 1037 CIFLYIKGHLAPSSTDSGSSGNAIIDFY*GMELYPRIGKNFDVKVFTNCRFGMMS 873
CI LYIKGH+APSS+DSGSSGN I+DFY GMELYPRIGK+FD+KVFTNCRFGMMS
Sbjct: 153 CILLYIKGHVAPSSSDSGSSGNVILDFYWGMELYPRIGKHFDIKVFTNCRFGMMS 207
Score = 74.7 bits (182), Expect(3) = 7e-38
Identities = 31/40 (77%), Positives = 37/40 (92%)
Frame = -3
Query: 837 RDWTSSDSMLVNMALMVMYVTKFFWWEAGYWNTMDIAHDR 718
++ T +DS+LVN ALM++YVTKFFWWEAGYWNTMDIAHDR
Sbjct: 222 KNGTLADSLLVNTALMLVYVTKFFWWEAGYWNTMDIAHDR 261
Score = 25.4 bits (54), Expect(3) = 7e-38
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2
Query: 871 WAVLAVTYRIKQ 836
WAVLAVTY IKQ
Sbjct: 208 WAVLAVTYCIKQ 219
>ref|XP_004141748.1| PREDICTED: 7-dehydrocholesterol reductase-like [Cucumis sativus]
Length = 435
Score = 144 bits (364), Expect(2) = 2e-49
Identities = 64/79 (81%), Positives = 71/79 (89%)
Frame = -2
Query: 358 IYVN*DCDRQRQEFRKKNGKCLIWDRFPSKIVASYQTGNGETKTSLLLTSGWWGLARHFH 179
IY+N DCDRQRQEFR+KNGKCL+W + PSKIVA+Y T GET+TSLLLTSGWWGLARHFH
Sbjct: 308 IYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVATYTTTTGETRTSLLLTSGWWGLARHFH 367
Query: 178 YVPEILSAFFWTVTALFIH 122
YVPEIL+AFFWTV ALF H
Sbjct: 368 YVPEILAAFFWTVPALFHH 386
Score = 79.0 bits (193), Expect(2) = 2e-49
Identities = 36/46 (78%), Positives = 39/46 (84%), Gaps = 4/46 (8%)
Frame = -1
Query: 485 AGFYICWGCLVWVPSIYPSPGMYL----VNLGTQLALFILAAGLLC 360
AGFYICWGCLVWVPS+Y SPGMYL VNLGTQLA+ IL AG+LC
Sbjct: 262 AGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSILVAGILC 307
Score = 105 bits (261), Expect(3) = 7e-38
Identities = 47/55 (85%), Positives = 52/55 (94%)
Frame = -1
Query: 1037 CIFLYIKGHLAPSSTDSGSSGNAIIDFY*GMELYPRIGKNFDVKVFTNCRFGMMS 873
CI LYIKGH+APSS+DSGSSGN I+DFY GMELYPRIGK+FD+KVFTNCRFGMMS
Sbjct: 153 CILLYIKGHVAPSSSDSGSSGNVILDFYWGMELYPRIGKHFDIKVFTNCRFGMMS 207
Score = 74.7 bits (182), Expect(3) = 7e-38
Identities = 31/40 (77%), Positives = 37/40 (92%)
Frame = -3
Query: 837 RDWTSSDSMLVNMALMVMYVTKFFWWEAGYWNTMDIAHDR 718
++ T +DS+LVN ALM++YVTKFFWWEAGYWNTMDIAHDR
Sbjct: 222 KNGTLADSLLVNTALMLVYVTKFFWWEAGYWNTMDIAHDR 261
Score = 25.4 bits (54), Expect(3) = 7e-38
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2
Query: 871 WAVLAVTYRIKQ 836
WAVLAVTY IKQ
Sbjct: 208 WAVLAVTYCIKQ 219
>ref|XP_002268455.2| PREDICTED: 7-dehydrocholesterol reductase-like [Vitis vinifera]
Length = 561
Score = 142 bits (357), Expect(2) = 2e-49
Identities = 63/79 (79%), Positives = 71/79 (89%)
Frame = -2
Query: 358 IYVN*DCDRQRQEFRKKNGKCLIWDRFPSKIVASYQTGNGETKTSLLLTSGWWGLARHFH 179
IY+N DCDRQRQEFR+ NGKCL+W + PSKIVASY T +GETK+SLLLTSGWWGL+RHFH
Sbjct: 307 IYINYDCDRQRQEFRRTNGKCLVWGKAPSKIVASYTTTSGETKSSLLLTSGWWGLSRHFH 366
Query: 178 YVPEILSAFFWTVTALFIH 122
YVPEIL+AFFWTV ALF H
Sbjct: 367 YVPEILAAFFWTVPALFGH 385
Score = 81.3 bits (199), Expect(2) = 2e-49
Identities = 37/46 (80%), Positives = 40/46 (86%), Gaps = 4/46 (8%)
Frame = -1
Query: 485 AGFYICWGCLVWVPSIYPSPGMYL----VNLGTQLALFILAAGLLC 360
AGFYICWGCLVWVPSIY SPGMYL VNLGTQLA++ILAAG+ C
Sbjct: 261 AGFYICWGCLVWVPSIYTSPGMYLVNHPVNLGTQLAIYILAAGITC 306
Score = 114 bits (285), Expect(2) = 2e-38
Identities = 59/81 (72%), Positives = 65/81 (80%), Gaps = 3/81 (3%)
Frame = -1
Query: 1037 CIFLYIKGHLAPSSTDSGSSGNAIIDFY*GMELYPRIGKNFDVKVFTNCRFGMMS---LG 867
CIFLYIKGH+APSSTDSGSSGN IIDFY GMELYPRIGKNFD+KVFTNCRFGMMS L
Sbjct: 152 CIFLYIKGHVAPSSTDSGSSGNIIIDFYWGMELYPRIGKNFDIKVFTNCRFGMMSWAVLA 211
Query: 866 SSCCDISYQAETGQVATLCLL 804
+ C Y+ E G+VA L+
Sbjct: 212 VTYCIKQYE-ENGKVADSMLV 231
Score = 72.4 bits (176), Expect(2) = 2e-38
Identities = 30/35 (85%), Positives = 33/35 (94%)
Frame = -3
Query: 822 SDSMLVNMALMVMYVTKFFWWEAGYWNTMDIAHDR 718
+DSMLVN LM++YVTKFFWWEAGYWNTMDIAHDR
Sbjct: 226 ADSMLVNTILMLVYVTKFFWWEAGYWNTMDIAHDR 260
>emb|CBI18435.3| unnamed protein product [Vitis vinifera]
Length = 418
Score = 142 bits (357), Expect(2) = 2e-49
Identities = 63/79 (79%), Positives = 71/79 (89%)
Frame = -2
Query: 358 IYVN*DCDRQRQEFRKKNGKCLIWDRFPSKIVASYQTGNGETKTSLLLTSGWWGLARHFH 179
IY+N DCDRQRQEFR+ NGKCL+W + PSKIVASY T +GETK+SLLLTSGWWGL+RHFH
Sbjct: 291 IYINYDCDRQRQEFRRTNGKCLVWGKAPSKIVASYTTTSGETKSSLLLTSGWWGLSRHFH 350
Query: 178 YVPEILSAFFWTVTALFIH 122
YVPEIL+AFFWTV ALF H
Sbjct: 351 YVPEILAAFFWTVPALFGH 369
Score = 81.3 bits (199), Expect(2) = 2e-49
Identities = 37/46 (80%), Positives = 40/46 (86%), Gaps = 4/46 (8%)
Frame = -1
Query: 485 AGFYICWGCLVWVPSIYPSPGMYL----VNLGTQLALFILAAGLLC 360
AGFYICWGCLVWVPSIY SPGMYL VNLGTQLA++ILAAG+ C
Sbjct: 245 AGFYICWGCLVWVPSIYTSPGMYLVNHPVNLGTQLAIYILAAGITC 290
Score = 114 bits (285), Expect(2) = 2e-38
Identities = 59/81 (72%), Positives = 65/81 (80%), Gaps = 3/81 (3%)
Frame = -1
Query: 1037 CIFLYIKGHLAPSSTDSGSSGNAIIDFY*GMELYPRIGKNFDVKVFTNCRFGMMS---LG 867
CIFLYIKGH+APSSTDSGSSGN IIDFY GMELYPRIGKNFD+KVFTNCRFGMMS L
Sbjct: 136 CIFLYIKGHVAPSSTDSGSSGNIIIDFYWGMELYPRIGKNFDIKVFTNCRFGMMSWAVLA 195
Query: 866 SSCCDISYQAETGQVATLCLL 804
+ C Y+ E G+VA L+
Sbjct: 196 VTYCIKQYE-ENGKVADSMLV 215
Score = 72.4 bits (176), Expect(2) = 2e-38
Identities = 30/35 (85%), Positives = 33/35 (94%)
Frame = -3
Query: 822 SDSMLVNMALMVMYVTKFFWWEAGYWNTMDIAHDR 718
+DSMLVN LM++YVTKFFWWEAGYWNTMDIAHDR
Sbjct: 210 ADSMLVNTILMLVYVTKFFWWEAGYWNTMDIAHDR 244