BLASTX nr result
ID: Coptis25_contig00013359
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00013359
(1226 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN68579.1| hypothetical protein VITISV_034891 [Vitis vinifera] 414 e-113
ref|XP_002276856.1| PREDICTED: putative tRNA pseudouridine synth... 412 e-113
emb|CBI15850.3| unnamed protein product [Vitis vinifera] 405 e-111
ref|XP_002517734.1| pseudouridylate synthase, putative [Ricinus ... 385 e-105
ref|XP_002319961.1| predicted protein [Populus trichocarpa] gi|2... 383 e-104
>emb|CAN68579.1| hypothetical protein VITISV_034891 [Vitis vinifera]
Length = 602
Score = 414 bits (1065), Expect = e-113
Identities = 217/334 (64%), Positives = 261/334 (78%), Gaps = 14/334 (4%)
Frame = -2
Query: 1039 DESIQEVTGDKWESFRKKKVVMRVGYVGTNYRGLQIQRDETSFTTVEAELEKAIFKAGGI 860
D+S+Q KWESFRKKKVVMRVGYVGTNYRGLQ+QRDE+S +T+E ELE A+FKAGGI
Sbjct: 51 DQSVQ-----KWESFRKKKVVMRVGYVGTNYRGLQMQRDESSLSTIEGELETAVFKAGGI 105
Query: 859 RESNYGNLHKIGWARSSRTDKGVHSLSTTIALKMEIPENAWKEDPCGIALARCINSNLPS 680
R+SNYGNLHKIGW RSSRTDKGVHSL+T I+LKMEIP NAWKEDP GIALA +N +LP+
Sbjct: 106 RDSNYGNLHKIGWDRSSRTDKGVHSLATMISLKMEIPANAWKEDPNGIALANYVNFHLPN 165
Query: 679 DVKVFSVLPSQRSFDARRECTVRKYSYLLPSEIIGITSDFSSSEIDHHISEFDNILRSFE 500
++KVFS+LPSQRSFDARREC +R+YSYLLP+EIIGI S+ S+SEIDHHI+EF++IL +FE
Sbjct: 166 NIKVFSILPSQRSFDARRECDLRRYSYLLPAEIIGIKSNSSASEIDHHIAEFNDILNTFE 225
Query: 499 GDRPFHNYTIRSNYR-----RQSPGKG------ESPKQALASDSESSD-ELKQCVVGVAA 356
G+ PF NYTIRS YR +QSPG G +S +A S+ E SD E V GV +
Sbjct: 226 GEHPFQNYTIRSKYRKQFPAKQSPGNGGVFRRAKSSGEASTSEFEVSDGEENSGVNGVIS 285
Query: 355 GDF-TGDSLSSSTSDPGV-HSVVTCEKHGDGLKDQGSIAPVRARWLYEPDERDRITASHF 182
D+ G++ +SS S V H V + + GLK+Q S VRARWL+ PDERDRI+ASHF
Sbjct: 286 SDYDEGENQNSSESTSFVEHPVGSSNNNEKGLKEQSSKLVVRARWLHGPDERDRISASHF 345
Query: 181 RKIHCCSCGKLERSSGKDYIEISICGESFMLHQV 80
RKI CSCGKLE+ G DY+E+SICGESFMLHQ+
Sbjct: 346 RKIFNCSCGKLEKFLGLDYVELSICGESFMLHQI 379
>ref|XP_002276856.1| PREDICTED: putative tRNA pseudouridine synthase-like [Vitis vinifera]
Length = 564
Score = 412 bits (1059), Expect = e-113
Identities = 215/334 (64%), Positives = 260/334 (77%), Gaps = 14/334 (4%)
Frame = -2
Query: 1039 DESIQEVTGDKWESFRKKKVVMRVGYVGTNYRGLQIQRDETSFTTVEAELEKAIFKAGGI 860
D+S+Q KWESFRKKKVVMRVGYVGTNYRGLQ+QRDE+S +T+E ELE A+FKAGGI
Sbjct: 51 DQSVQ-----KWESFRKKKVVMRVGYVGTNYRGLQMQRDESSLSTIEGELETAVFKAGGI 105
Query: 859 RESNYGNLHKIGWARSSRTDKGVHSLSTTIALKMEIPENAWKEDPCGIALARCINSNLPS 680
R+SNYGNLHKIGW RSSRTDKGVHSL+T I+LKMEIP NAWKEDP GIALA +N +LP+
Sbjct: 106 RDSNYGNLHKIGWDRSSRTDKGVHSLATMISLKMEIPANAWKEDPNGIALANYVNFHLPN 165
Query: 679 DVKVFSVLPSQRSFDARRECTVRKYSYLLPSEIIGITSDFSSSEIDHHISEFDNILRSFE 500
++KVFS+LPSQRSFDARREC +R+YSYLLP+EIIGI S+ S+SEIDHHI+EF++IL +FE
Sbjct: 166 NIKVFSILPSQRSFDARRECDLRRYSYLLPAEIIGIKSNSSASEIDHHIAEFNDILNTFE 225
Query: 499 GDRPFHNYTIRSNYR-----RQSPGKG------ESPKQALASDSESSD-ELKQCVVGVAA 356
G+ PF NYTIRS YR +QSPG G +S +A S+ E SD E + GV +
Sbjct: 226 GEHPFQNYTIRSKYRKQFPAKQSPGNGGVFRRAKSSGEASTSEFEVSDGEENSGINGVIS 285
Query: 355 GDF-TGDSLSSSTSDPGV-HSVVTCEKHGDGLKDQGSIAPVRARWLYEPDERDRITASHF 182
D+ G++ +SS S V H V + + GLK+Q VRARWL+ PDERDRI+ASHF
Sbjct: 286 SDYDEGENQNSSESTSFVEHPVGSSNNNEKGLKEQSPKLVVRARWLHGPDERDRISASHF 345
Query: 181 RKIHCCSCGKLERSSGKDYIEISICGESFMLHQV 80
RKI CSCGKLE+ G DY+E+SICGESFMLHQ+
Sbjct: 346 RKIFNCSCGKLEKFLGLDYVELSICGESFMLHQI 379
>emb|CBI15850.3| unnamed protein product [Vitis vinifera]
Length = 495
Score = 405 bits (1042), Expect = e-111
Identities = 207/322 (64%), Positives = 251/322 (77%), Gaps = 2/322 (0%)
Frame = -2
Query: 1039 DESIQEVTGDKWESFRKKKVVMRVGYVGTNYRGLQIQRDETSFTTVEAELEKAIFKAGGI 860
D+S+Q KWESFRKKKVVMRVGYVGTNYRGLQ+QRDE+S +T+E ELE A+FKAGGI
Sbjct: 51 DQSVQ-----KWESFRKKKVVMRVGYVGTNYRGLQMQRDESSLSTIEGELETAVFKAGGI 105
Query: 859 RESNYGNLHKIGWARSSRTDKGVHSLSTTIALKMEIPENAWKEDPCGIALARCINSNLPS 680
R+SNYGNLHKIGW RSSRTDKGVHSL+T I+LKMEIP NAWKEDP GIALA +N +LP+
Sbjct: 106 RDSNYGNLHKIGWDRSSRTDKGVHSLATMISLKMEIPANAWKEDPNGIALANYVNFHLPN 165
Query: 679 DVKVFSVLPSQRSFDARRECTVRKYSYLLPSEIIGITSDFSSSEIDHHISEFDNILRSFE 500
++KVFS+LPSQRSFDARREC +R+YSYLLP+EIIGI S+ S+SEIDHHI+EF++IL +FE
Sbjct: 166 NIKVFSILPSQRSFDARRECDLRRYSYLLPAEIIGIKSNSSASEIDHHIAEFNDILNTFE 225
Query: 499 GDRPFHNYTIRSNYRRQSPGKGESPKQALASDSESSDELKQCVVGVAAGDF-TGDSLSSS 323
G+ PF NYTIRS YR+Q P K + ++SS V + D+ G++ +SS
Sbjct: 226 GEHPFQNYTIRSKYRKQFPAKQSPGNGGVFRRAKSS---------VISSDYDEGENQNSS 276
Query: 322 TSDPGV-HSVVTCEKHGDGLKDQGSIAPVRARWLYEPDERDRITASHFRKIHCCSCGKLE 146
S V H V + + GLK+Q VRARWL+ PDERDRI+ASHFRKI CSCGKLE
Sbjct: 277 ESTSFVEHPVGSSNNNEKGLKEQSPKLVVRARWLHGPDERDRISASHFRKIFNCSCGKLE 336
Query: 145 RSSGKDYIEISICGESFMLHQV 80
+ G DY+E+SICGESFMLHQ+
Sbjct: 337 KFLGLDYVELSICGESFMLHQI 358
>ref|XP_002517734.1| pseudouridylate synthase, putative [Ricinus communis]
gi|223543132|gb|EEF44666.1| pseudouridylate synthase,
putative [Ricinus communis]
Length = 377
Score = 385 bits (990), Expect = e-105
Identities = 196/324 (60%), Positives = 241/324 (74%), Gaps = 6/324 (1%)
Frame = -2
Query: 1036 ESIQEVTGDKWESFRKKKVVMRVGYVGTNYRGLQIQRDETSFTTVEAELEKAIFKAGGIR 857
+S + KWE FRKKKVV+RVGYVGT+YRGLQ Q DE S +T+EAELE AIFKAGGI
Sbjct: 38 QSHAHILSQKWEPFRKKKVVLRVGYVGTDYRGLQKQEDEHSLSTIEAELECAIFKAGGIL 97
Query: 856 ESNYGNLHKIGWARSSRTDKGVHSLSTTIALKMEIPENAWKEDPCGIALARCINSNLPSD 677
+SNYGNLHKI WARSSRTDKGVHSL+T I++KMEIPENAWKEDP GIALA C+N LP +
Sbjct: 98 DSNYGNLHKIAWARSSRTDKGVHSLATMISMKMEIPENAWKEDPNGIALAECVNYYLPDN 157
Query: 676 VKVFSVLPSQRSFDARRECTVRKYSYLLPSEIIGITSDFSSSEIDHHISEFDNILRSFEG 497
+++FS+LPSQ+SFD RREC +RKYSYLLP+EIIGI F+S+EID+HIS+F++IL +FEG
Sbjct: 158 IRIFSILPSQKSFDPRRECNLRKYSYLLPAEIIGIKGHFTSAEIDNHISDFNDILNAFEG 217
Query: 496 DRPFHNYTIRSNYRRQSPG--KGESPKQALASDSESSDELKQCVVGVAAGDFTGDSLSSS 323
+ PFHNYTIRS YR+Q P G PK+A +S S+ E ++ D+ D ++
Sbjct: 218 EHPFHNYTIRSKYRKQYPTAITGRVPKRARSSSERSTSESEK---SDGEEDYEIDEINIE 274
Query: 322 TSDPGVHSV----VTCEKHGDGLKDQGSIAPVRARWLYEPDERDRITASHFRKIHCCSCG 155
+ + S + ++ G L+ Q A + ARWLYEPDE DRI ASHFRKI CSCG
Sbjct: 275 DHNGSIQSSSKFRFSNQQKGGVLEQQQYNAGILARWLYEPDETDRIGASHFRKIFRCSCG 334
Query: 154 KLERSSGKDYIEISICGESFMLHQ 83
KLE S G D++EISI GESFMLHQ
Sbjct: 335 KLETSKGFDFVEISIWGESFMLHQ 358
>ref|XP_002319961.1| predicted protein [Populus trichocarpa] gi|222858337|gb|EEE95884.1|
predicted protein [Populus trichocarpa]
Length = 445
Score = 383 bits (983), Expect = e-104
Identities = 194/321 (60%), Positives = 241/321 (75%), Gaps = 1/321 (0%)
Frame = -2
Query: 1039 DESIQEVTGDKWESFRKKKVVMRVGYVGTNYRGLQIQRDETSFTTVEAELEKAIFKAGGI 860
D + + KWES RKKKV +RVGYVGT+YRGLQ Q D+ S T+E ELE+AI++AGGI
Sbjct: 15 DSNSNQSLHSKWESIRKKKVALRVGYVGTDYRGLQKQHDQHSLLTIEGELERAIYEAGGI 74
Query: 859 RESNYGNLHKIGWARSSRTDKGVHSLSTTIALKMEIPENAWKEDPCGIALARCINSNLPS 680
RESNYGNL+KI WARSSRTDKGVHSL+TTI +KMEIPENAWK+D G+ LA+ +NS LP
Sbjct: 75 RESNYGNLNKISWARSSRTDKGVHSLATTITMKMEIPENAWKDDLYGMVLAKHVNSYLPD 134
Query: 679 DVKVFSVLPSQRSFDARRECTVRKYSYLLPSEIIGITSDFSSSEIDHHISEFDNILRSFE 500
++V S+LP+Q+SFD RREC +RKYSYLLP+E+IGI S FS +EID HIS+F+NIL +FE
Sbjct: 135 SIRVLSILPAQKSFDPRRECDIRKYSYLLPAEVIGIKSHFSMAEIDDHISDFNNILNAFE 194
Query: 499 GDRPFHNYTIRSNYRRQSPGK-GESPKQALASDSESSDELKQCVVGVAAGDFTGDSLSSS 323
GD PFHNYT+RS YRR+ P K G K+A +S S+ ++ V + + +SL SS
Sbjct: 195 GDHPFHNYTVRSKYRRKYPTKNGHVSKRARSSWGTSASGSEENHV-----ELSQNSLESS 249
Query: 322 TSDPGVHSVVTCEKHGDGLKDQGSIAPVRARWLYEPDERDRITASHFRKIHCCSCGKLER 143
C+ +GD LKDQ S P+RARWL+EPDE DRI A+HFRKI CSCGKLE+
Sbjct: 250 ----------ICDLNGDILKDQSSNLPLRARWLHEPDETDRIGAAHFRKIFRCSCGKLEK 299
Query: 142 SSGKDYIEISICGESFMLHQV 80
S G D++EISI GESFMLHQ+
Sbjct: 300 SLGFDFVEISIWGESFMLHQI 320