BLASTX nr result
ID: Coptis25_contig00012990
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00012990
(2000 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002329323.1| 4-coumarate-coa ligase [Populus trichocarpa]... 802 0.0
ref|XP_002523698.1| AMP dependent CoA ligase, putative [Ricinus ... 801 0.0
ref|XP_002305282.1| 4-coumarate-coa ligase [Populus trichocarpa]... 797 0.0
gb|AFD33347.1| acyl-activating enzyme 3 [Cannabis sativa] 778 0.0
ref|XP_002872738.1| hypothetical protein ARALYDRAFT_490166 [Arab... 778 0.0
>ref|XP_002329323.1| 4-coumarate-coa ligase [Populus trichocarpa]
gi|224127626|ref|XP_002329324.1| acyl:coa ligase [Populus
trichocarpa] gi|222870777|gb|EEF07908.1| 4-coumarate-coa
ligase [Populus trichocarpa] gi|222870778|gb|EEF07909.1|
acyl:coa ligase [Populus trichocarpa]
Length = 543
Score = 802 bits (2072), Expect = 0.0
Identities = 391/543 (72%), Positives = 463/543 (85%)
Frame = -2
Query: 1846 MEKSGYGRDGIYRSLRPPLILPRDPNLSLVSFLFRNIHSYSDTPALIDSDTSQTLTFTQL 1667
MEKSGYGRDGIYRSLRP L+LPRD NLSLVSFLFRN +SY PALID+D S TL+F++L
Sbjct: 1 MEKSGYGRDGIYRSLRPTLVLPRDSNLSLVSFLFRNSNSYPHKPALIDADLSITLSFSEL 60
Query: 1666 KSTVAKLAHSFLQLGIKKGDVVLIFAPNSIQFPLTFLAVVSIGAIATTVNPLYTVHELAK 1487
KS V K AH L LGI K DV+LIFAPNS QFP FLA+ SIGA+ATT NPLYT EL+K
Sbjct: 61 KSIVIKFAHGLLNLGISKNDVILIFAPNSYQFPTCFLAITSIGAVATTANPLYTTSELSK 120
Query: 1486 QVKDSNPKLVITVSELWDKTKGFNLPAVILGTEMVPXXXXXNRKVISYSELIQMAGPVTE 1307
Q+KDSNPKLVITV ELWDK KGFNLPAV LG + V ++ S+ L+++ G +E
Sbjct: 121 QIKDSNPKLVITVPELWDKVKGFNLPAVFLGPKEVSLPLESGSRIRSFHSLVELGGSNSE 180
Query: 1306 FPVSIVKQSDTAALLYSSGTTGTSKGVILTHRNFISASLMVTADQEFGGERNNVFLCFLP 1127
FPVS VKQSD A LLYSSGTTG SKGVILTH NFI+ASLMV+ DQ GE +NVFLCFLP
Sbjct: 181 FPVSDVKQSDIATLLYSSGTTGVSKGVILTHGNFIAASLMVSMDQVMAGEIHNVFLCFLP 240
Query: 1126 MFHIFGLSIITYSQLQRGNTVISMGRFDIEMVLRSVEKYKVTNLFVVPPVMIAFAKLSVV 947
MFH+FGL++ITYSQLQ GN V+SMG+F+ EMVLR++EKY+VT+++VVPPV++A +K ++V
Sbjct: 241 MFHVFGLAVITYSQLQMGNAVVSMGKFEFEMVLRTIEKYRVTHMWVVPPVILALSKQNLV 300
Query: 946 KKYDLSSLKQLGSGAAPLGKDIMEECAKNIPGAAVVQGYGLTESCGIVSLENAYEGSRHS 767
KKYDLSSL+ +GSGAAPLGKD+M+ECAKN+P ++QG+G+TE+CGIVSLE+ G RHS
Sbjct: 301 KKYDLSSLRNIGSGAAPLGKDLMKECAKNLPDTTIIQGFGMTETCGIVSLEDPRIGVRHS 360
Query: 766 GSTGTLVPGVESKIVSVDTMKALPPTALGEIWIRGPNMMQGYFNNPQATKLTIDKQGWIH 587
GS G L G+E++I+SV+T K LPP LGEIW+RGPNMM+GYFNNPQATK TIDK+GW+H
Sbjct: 361 GSAGILNAGIEAQIISVETAKPLPPNQLGEIWVRGPNMMRGYFNNPQATKDTIDKKGWVH 420
Query: 586 TGDLGYFDERGQLFVVDRIKELIKYKGFQVAPAELEGLLLTHPEILDAVVIPFPDAEAGE 407
TGDLGYFD+ GQLFVVDRIKELIKYKGFQVAPAELEGLL++HPEILDAVV P+PDAEAGE
Sbjct: 421 TGDLGYFDDDGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVTPYPDAEAGE 480
Query: 406 VPIAYVVRSPNSSLNEETVQNFIAKQVAPFKRLRRVTFINTVPKSASGKILRRELIEKVR 227
VP+AYVVRSPNS+L EE VQ FI+ QVAPFKRLR+VTFIN+VPKSASGKILRREL++KV+
Sbjct: 481 VPVAYVVRSPNSALTEEDVQKFISDQVAPFKRLRKVTFINSVPKSASGKILRRELVQKVK 540
Query: 226 SKV 218
SK+
Sbjct: 541 SKM 543
>ref|XP_002523698.1| AMP dependent CoA ligase, putative [Ricinus communis]
gi|223537002|gb|EEF38638.1| AMP dependent CoA ligase,
putative [Ricinus communis]
Length = 542
Score = 801 bits (2069), Expect = 0.0
Identities = 395/543 (72%), Positives = 465/543 (85%)
Frame = -2
Query: 1846 MEKSGYGRDGIYRSLRPPLILPRDPNLSLVSFLFRNIHSYSDTPALIDSDTSQTLTFTQL 1667
MEKSGYGRDGIYRSLRP L+LP+DPNLS+VSFLFRN +SY PALID+D S+TL+F+QL
Sbjct: 1 MEKSGYGRDGIYRSLRPLLVLPKDPNLSMVSFLFRNCNSYPHKPALIDADLSKTLSFSQL 60
Query: 1666 KSTVAKLAHSFLQLGIKKGDVVLIFAPNSIQFPLTFLAVVSIGAIATTVNPLYTVHELAK 1487
KS V K++H L+LGI K DVVLIFAPNS QFP+ FLA+ SIGAIATT NPLYT E++K
Sbjct: 61 KSIVIKVSHGLLKLGISKNDVVLIFAPNSYQFPICFLAITSIGAIATTANPLYTTTEISK 120
Query: 1486 QVKDSNPKLVITVSELWDKTKGFNLPAVILGTEMVPXXXXXNRKVISYSELIQMAGPVTE 1307
Q+KDSNPKLVITV ELW+K K FNLPAV LG + +R + S+ L+++ G +E
Sbjct: 121 QIKDSNPKLVITVPELWNKVKDFNLPAVFLGAKESLLIEPNSR-IKSFDHLVELGGSNSE 179
Query: 1306 FPVSIVKQSDTAALLYSSGTTGTSKGVILTHRNFISASLMVTADQEFGGERNNVFLCFLP 1127
FP VKQ+D A LLYSSGTTG SKGVILTH NFI+AS M+T DQE GE +NVFLCFLP
Sbjct: 180 FPTINVKQTDIATLLYSSGTTGISKGVILTHGNFIAASQMITMDQEIAGELHNVFLCFLP 239
Query: 1126 MFHIFGLSIITYSQLQRGNTVISMGRFDIEMVLRSVEKYKVTNLFVVPPVMIAFAKLSVV 947
MFH+FGL++I YSQLQ GN V+SMG+FD E+VL++VEKY++T+L+VVPPV++A AK S+V
Sbjct: 240 MFHVFGLAVIAYSQLQTGNAVVSMGKFDFELVLKAVEKYRITHLWVVPPVILALAKQSLV 299
Query: 946 KKYDLSSLKQLGSGAAPLGKDIMEECAKNIPGAAVVQGYGLTESCGIVSLENAYEGSRHS 767
KKYDLSSL+ +GSGAAPL K++MEECAK IP AA+ QGYG+TE+ GIVS+EN G RHS
Sbjct: 300 KKYDLSSLQHVGSGAAPLSKELMEECAKTIPHAAIAQGYGMTETTGIVSVENPRIGVRHS 359
Query: 766 GSTGTLVPGVESKIVSVDTMKALPPTALGEIWIRGPNMMQGYFNNPQATKLTIDKQGWIH 587
GS GTL G+E++I+SVDT+K LPP LGEIW+RGPNMM+GYFNNPQATK TIDK+GW+H
Sbjct: 360 GSAGTLAAGIEAQIISVDTLKPLPPNQLGEIWVRGPNMMRGYFNNPQATKQTIDKKGWLH 419
Query: 586 TGDLGYFDERGQLFVVDRIKELIKYKGFQVAPAELEGLLLTHPEILDAVVIPFPDAEAGE 407
TGDLGYFDE G+L+VVDRIKELIKYKGFQVAPAELEGLL++HPE+LDAVVIPFPDAEAGE
Sbjct: 420 TGDLGYFDEDGKLYVVDRIKELIKYKGFQVAPAELEGLLVSHPELLDAVVIPFPDAEAGE 479
Query: 406 VPIAYVVRSPNSSLNEETVQNFIAKQVAPFKRLRRVTFINTVPKSASGKILRRELIEKVR 227
VP+AYVVRSPNSSL EE VQ +IA QVAPFKRLRRVTFINTVPKSASGKILRRELIEKV+
Sbjct: 480 VPVAYVVRSPNSSLTEEEVQKYIADQVAPFKRLRRVTFINTVPKSASGKILRRELIEKVK 539
Query: 226 SKV 218
SK+
Sbjct: 540 SKL 542
>ref|XP_002305282.1| 4-coumarate-coa ligase [Populus trichocarpa]
gi|222848246|gb|EEE85793.1| 4-coumarate-coa ligase
[Populus trichocarpa]
Length = 543
Score = 797 bits (2058), Expect = 0.0
Identities = 386/543 (71%), Positives = 463/543 (85%)
Frame = -2
Query: 1846 MEKSGYGRDGIYRSLRPPLILPRDPNLSLVSFLFRNIHSYSDTPALIDSDTSQTLTFTQL 1667
MEKSGYGRDGIYRSLRP L+LP+DPNLSLVSFLFRN +SY PALID+D S TL+F++L
Sbjct: 1 MEKSGYGRDGIYRSLRPKLVLPKDPNLSLVSFLFRNSNSYPHKPALIDADLSITLSFSEL 60
Query: 1666 KSTVAKLAHSFLQLGIKKGDVVLIFAPNSIQFPLTFLAVVSIGAIATTVNPLYTVHELAK 1487
KS V K+AH L LGI K DVVLIFAPNS QFP+ FLA+ S GAIATT NPLYT EL+K
Sbjct: 61 KSIVIKVAHGLLNLGISKNDVVLIFAPNSYQFPICFLAITSTGAIATTANPLYTTSELSK 120
Query: 1486 QVKDSNPKLVITVSELWDKTKGFNLPAVILGTEMVPXXXXXNRKVISYSELIQMAGPVTE 1307
Q+KDSNPKL+ITV ELWDK KGFNLPAV LG + V ++ S+ L+ + G ++
Sbjct: 121 QIKDSNPKLIITVPELWDKVKGFNLPAVFLGPKRVSLPLESGSRITSFDSLVGLGGSNSQ 180
Query: 1306 FPVSIVKQSDTAALLYSSGTTGTSKGVILTHRNFISASLMVTADQEFGGERNNVFLCFLP 1127
FP S VKQSD + LLYSSGTTG SKGVILTH NFI+ASLMVT DQ GE ++VFLCFLP
Sbjct: 181 FPSSNVKQSDISTLLYSSGTTGVSKGVILTHGNFIAASLMVTMDQAMAGEMHDVFLCFLP 240
Query: 1126 MFHIFGLSIITYSQLQRGNTVISMGRFDIEMVLRSVEKYKVTNLFVVPPVMIAFAKLSVV 947
MFH+FGL++ITYSQLQ GN V+SMG+F+ EMVL+++EK++VT+++VVPPV++A AK +V
Sbjct: 241 MFHVFGLAVITYSQLQMGNAVVSMGKFEFEMVLKTIEKHRVTDMWVVPPVVLALAKQDMV 300
Query: 946 KKYDLSSLKQLGSGAAPLGKDIMEECAKNIPGAAVVQGYGLTESCGIVSLENAYEGSRHS 767
KKYD+SSL+ +GSGAAPLGKD+M+ECAKN+P + QGYG+TE+CGIVS+E++ G RHS
Sbjct: 301 KKYDISSLRNIGSGAAPLGKDLMKECAKNLPDTIISQGYGMTETCGIVSVEDSRLGVRHS 360
Query: 766 GSTGTLVPGVESKIVSVDTMKALPPTALGEIWIRGPNMMQGYFNNPQATKLTIDKQGWIH 587
GS G L G+E++IVSV+T+K LPP LGEIW+RGPNMM+GYFNNPQATK T+DK+GW+H
Sbjct: 361 GSAGILAAGIEAQIVSVETLKPLPPNQLGEIWVRGPNMMRGYFNNPQATKDTMDKKGWVH 420
Query: 586 TGDLGYFDERGQLFVVDRIKELIKYKGFQVAPAELEGLLLTHPEILDAVVIPFPDAEAGE 407
TGD+GYFD+ GQLFVVDRIKELIKYKGFQVAPAELEGLL++HP+ILDAVVIP+PDAEAGE
Sbjct: 421 TGDVGYFDDDGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPQILDAVVIPYPDAEAGE 480
Query: 406 VPIAYVVRSPNSSLNEETVQNFIAKQVAPFKRLRRVTFINTVPKSASGKILRRELIEKVR 227
VP+AYVVRSPNS+L E VQ FIA QVAPFKRLR+VTFINTVPKSASGKILRRELI++V+
Sbjct: 481 VPVAYVVRSPNSALTEVDVQKFIADQVAPFKRLRKVTFINTVPKSASGKILRRELIQQVK 540
Query: 226 SKV 218
SK+
Sbjct: 541 SKI 543
>gb|AFD33347.1| acyl-activating enzyme 3 [Cannabis sativa]
Length = 543
Score = 778 bits (2009), Expect = 0.0
Identities = 388/545 (71%), Positives = 464/545 (85%), Gaps = 2/545 (0%)
Frame = -2
Query: 1846 MEKSGYGRDGIYRSLRPPLILPRDPNLSLVSFLFRNIHSYSDTPALIDSDTSQTLTFTQL 1667
MEKSGYGRDGIYRSLRPPL LP + NLS+VSFLFRN SY PALIDS+T+Q L+F+
Sbjct: 1 MEKSGYGRDGIYRSLRPPLHLPNNNNLSMVSFLFRNSSSYPQKPALIDSETNQILSFSHF 60
Query: 1666 KSTVAKLAHSFLQLGIKKGDVVLIFAPNSIQFPLTFLAVVSIGAIATTVNPLYTVHELAK 1487
KSTV K++H FL LGIKK DVVLI+APNSI FP+ FL +++ GAIATT NPLYTV EL+K
Sbjct: 61 KSTVIKVSHGFLNLGIKKNDVVLIYAPNSIHFPVCFLGIIASGAIATTSNPLYTVSELSK 120
Query: 1486 QVKDSNPKLVITVSELWDKTKGFNLPAVILGTEMVPXXXXXNRKVISYSELIQMAGPV-T 1310
QVKDSNPKL+ITV +L +K KGFNLP +++G + KV+++++L+ + G +
Sbjct: 121 QVKDSNPKLIITVPQLLEKVKGFNLPTILIGPDSEQESSSD--KVMTFNDLVNLGGSSGS 178
Query: 1309 EFP-VSIVKQSDTAALLYSSGTTGTSKGVILTHRNFISASLMVTADQEFGGERNNVFLCF 1133
EFP V KQSDTAALLYSSGTTG SKGV+LTH+NFI++SLMVT +Q+ GE +NVFLCF
Sbjct: 179 EFPIVDDFKQSDTAALLYSSGTTGMSKGVVLTHKNFIASSLMVTMEQDLVGEMDNVFLCF 238
Query: 1132 LPMFHIFGLSIITYSQLQRGNTVISMGRFDIEMVLRSVEKYKVTNLFVVPPVMIAFAKLS 953
LPMFH+FGL+IITY+QLQRGNTVISM RFD+E +L+ VEKYKVT+L+VVPPV++A +K S
Sbjct: 239 LPMFHVFGLAIITYAQLQRGNTVISMARFDLEKMLKDVEKYKVTHLWVVPPVILALSKNS 298
Query: 952 VVKKYDLSSLKQLGSGAAPLGKDIMEECAKNIPGAAVVQGYGLTESCGIVSLENAYEGSR 773
+VKK++LSS+K +GSGAAPLGKD+MEEC+K +P V QGYG+TE+CGIVS+E+ G R
Sbjct: 299 MVKKFNLSSIKYIGSGAAPLGKDLMEECSKVVPYGIVAQGYGMTETCGIVSMEDIRGGKR 358
Query: 772 HSGSTGTLVPGVESKIVSVDTMKALPPTALGEIWIRGPNMMQGYFNNPQATKLTIDKQGW 593
+SGS G L GVE++IVSVDT+K LPP LGEIW++GPNMMQGYFNNPQATKLTIDK+GW
Sbjct: 359 NSGSAGMLASGVEAQIVSVDTLKPLPPNQLGEIWVKGPNMMQGYFNNPQATKLTIDKKGW 418
Query: 592 IHTGDLGYFDERGQLFVVDRIKELIKYKGFQVAPAELEGLLLTHPEILDAVVIPFPDAEA 413
+HTGDLGYFDE G L+VVDRIKELIKYKGFQVAPAELEGLL++HPEILDAVVIPFPDAEA
Sbjct: 419 VHTGDLGYFDEDGHLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEA 478
Query: 412 GEVPIAYVVRSPNSSLNEETVQNFIAKQVAPFKRLRRVTFINTVPKSASGKILRRELIEK 233
GEVP+AYVVRSPNSSL E V+ FIA QVA FKRLR+VTFIN+VPKSASGKILRRELI+K
Sbjct: 479 GEVPVAYVVRSPNSSLTENDVKKFIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQK 538
Query: 232 VRSKV 218
VRS +
Sbjct: 539 VRSNM 543
>ref|XP_002872738.1| hypothetical protein ARALYDRAFT_490166 [Arabidopsis lyrata subsp.
lyrata] gi|297318575|gb|EFH48997.1| hypothetical protein
ARALYDRAFT_490166 [Arabidopsis lyrata subsp. lyrata]
Length = 544
Score = 778 bits (2008), Expect = 0.0
Identities = 374/544 (68%), Positives = 470/544 (86%), Gaps = 1/544 (0%)
Frame = -2
Query: 1846 MEKSGYGRDGIYRSLRPPLILPRDPNLSLVSFLFRNIHSYSDTPALIDSDTSQTLTFTQL 1667
MEKSGYGRDGIYRSLRP L+LP+DPN SLVSFLFRN SY A+ DSD+ +L+F+QL
Sbjct: 1 MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDSGDSLSFSQL 60
Query: 1666 KSTVAKLAHSFLQLGIKKGDVVLIFAPNSIQFPLTFLAVVSIGAIATTVNPLYTVHELAK 1487
KS VA+LAH F +LG++K DVVLIFAPNS QFPL FLAV +IG + TT NPLYTV+E++K
Sbjct: 61 KSAVARLAHGFQRLGVRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120
Query: 1486 QVKDSNPKLVITVSELWDKTKGFNLPAVILGT-EMVPXXXXXNRKVISYSELIQMAGPVT 1310
Q+KDSNPK++I+V++L+DK KGFNLP V+LG+ + V N K++S++++++++ PV+
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFNLPVVLLGSKDSVQIPPGSNSKILSFNDVMELSEPVS 180
Query: 1309 EFPVSIVKQSDTAALLYSSGTTGTSKGVILTHRNFISASLMVTADQEFGGERNNVFLCFL 1130
++P +KQSDTAALLYSSGTTGTSKGV LTH NFI+ASLMVT DQ+ GE + VFLCFL
Sbjct: 181 DYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFL 240
Query: 1129 PMFHIFGLSIITYSQLQRGNTVISMGRFDIEMVLRSVEKYKVTNLFVVPPVMIAFAKLSV 950
PMFH+FGL++ITYSQLQRGN ++SM +F++E+VL+++EKY+VT+L+VVPPV +A +K S+
Sbjct: 241 PMFHVFGLTVITYSQLQRGNALVSMAKFELELVLKNIEKYRVTHLWVVPPVFLALSKQSI 300
Query: 949 VKKYDLSSLKQLGSGAAPLGKDIMEECAKNIPGAAVVQGYGLTESCGIVSLENAYEGSRH 770
VKK+DLSSLK +GSGAAPLGKD+MEEC +NIP ++QGYG+TE+CGIVS+E+ G R+
Sbjct: 301 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRN 360
Query: 769 SGSTGTLVPGVESKIVSVDTMKALPPTALGEIWIRGPNMMQGYFNNPQATKLTIDKQGWI 590
SGS G L PGVE++IVSV+T + PP GEIW+RGPNMM+GY NNPQATK TIDK+GW+
Sbjct: 361 SGSAGMLAPGVEAQIVSVETGNSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKGWV 420
Query: 589 HTGDLGYFDERGQLFVVDRIKELIKYKGFQVAPAELEGLLLTHPEILDAVVIPFPDAEAG 410
HTGDLGYF+E G L+VVDR+KELIKYKGFQVAPAELEGLL++HPEILDAVVIPFPD EAG
Sbjct: 421 HTGDLGYFNEDGNLYVVDRLKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDEEAG 480
Query: 409 EVPIAYVVRSPNSSLNEETVQNFIAKQVAPFKRLRRVTFINTVPKSASGKILRRELIEKV 230
EVPIA+VVRSPNSS+ E+ +Q FIAKQVAP+KRLRRV+FINTVPKSA+GKILRREL+++V
Sbjct: 481 EVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFINTVPKSAAGKILRRELVQQV 540
Query: 229 RSKV 218
RSK+
Sbjct: 541 RSKM 544