BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00012958
         (1801 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]    494   e-137
dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]       484   e-134
ref|XP_003523888.1| PREDICTED: cytochrome P450 82C4-like [Glycin...   484   e-134
ref|XP_002510298.1| cytochrome P450, putative [Ricinus communis]...   482   e-133
dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]       481   e-133

>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
          Length = 524

 Score =  494 bits (1273), Expect = e-137
 Identities = 243/513 (47%), Positives = 342/513 (66%), Gaps = 1/513 (0%)
 Frame = -1

Query: 1717 NKRXXXXXXXXXXXPILGHLHMLMGDELPHHVLGKLADKHGPAFLIHVGPHPELVVSSWE 1538
            NK+           P+LGHLH+    ELPH +L  +ADK+G AF +  G H  LVVS   
Sbjct: 34   NKKIRTAPMATGAWPVLGHLHLFGSGELPHKMLAAMADKYGSAFRMKFGKHTTLVVSDTR 93

Query: 1537 LAKECFTTNDKCFTNRPSNKAFKYLTYDQASVGFAPYGPLWIEMRKVSKSNLLSNQRIQM 1358
            + KECFTTND  F+NRPS KAF+ +TYD  SV F PYG  W E+RK+S   LLSN R+Q 
Sbjct: 94   IVKECFTTNDTLFSNRPSTKAFQLMTYDNESVAFTPYGSYWREIRKISTLKLLSNHRLQA 153

Query: 1357 QKQVRATEVDAFFNELYQSCXXXXXXXXXXXXXXXDKDGTVVGRGPVIVEMNKWFEELTL 1178
             K VRA+EV+  F  LY  C                      G  P++++M KWFEE++ 
Sbjct: 154  IKDVRASEVNVCFKTLYDQCKNPS------------------GSAPILIDMKKWFEEVSN 195

Query: 1177 NLVTRMVSGKEHVGTNARRGDSEAKYYKKVIDDAAHFMGNLVISDIFPSLGWLDYLLGHE 998
            N+V R++ G+++ G+   +G+ EA +YKKV+D+        + SD  P LG++D   G+ 
Sbjct: 196  NVVMRVIVGRQNFGSKIVQGEEEAIHYKKVMDELLRLASLSMFSDFAPLLGFVDIFQGNL 255

Query: 997  GSMKKTGKELDSIFSSWVKEHQQKRKVQGSSNKDPEEDFIDITLSAIEESQLHGLDTETF 818
             +MK+  K++D+I  +W++EH++K+    +S  + ++DF+D+ LS +EES+L G D +  
Sbjct: 256  SAMKRNAKKVDAILENWLEEHRKKK----NSVAESQQDFMDVMLSIVEESKLSGHDADAV 311

Query: 817  IKSICVGMILGGSDTTSVAXXXXXXXXLNNRDILEKAQDELDQHVGKERKVDDSDINNLV 638
            IK+ C+ MI+GG+DTT+V+        +NNR  L+KA++ELD  VGK+R+V+DSD+ NLV
Sbjct: 312  IKATCLAMIMGGTDTTAVSLTWIISLLMNNRHALKKAREELDALVGKDRQVEDSDLKNLV 371

Query: 637  YLGAIVKESMRLYQVGPLIEREATEDCNIGGFHVKKGSRLMVNIWKVQQDPTVWSDPSEF 458
            Y+ AIVKE+MR+Y +G L+ERE  EDC I GFHVK G+RL+VN+WK+Q+DP VW DP+EF
Sbjct: 372  YMNAIVKETMRMYPLGTLLERETKEDCEIDGFHVKGGTRLLVNVWKLQRDPNVWVDPTEF 431

Query: 457  RPERFLSGNLGMDVKGQHFELIPFGSGRRMCPGLGFALNVIHLVLARLLHSFDLATPLNA 278
            RPERFL+ N  +DV GQHFEL+PFG+GRR+CPG+ FAL  +HLVLARL+H +DL T    
Sbjct: 432  RPERFLTENADIDVGGQHFELLPFGAGRRVCPGVXFALQFMHLVLARLIHGYDLNTLNEE 491

Query: 277  KVDMTET-SSVTNYKGTPLQVLLTPRLKSTLYD 182
             VD+TE+     N+K +PL ++LTPRL   LY+
Sbjct: 492  NVDLTESPEGHVNHKASPLDLILTPRLHYKLYE 524


>dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 511

 Score =  484 bits (1247), Expect = e-134
 Identities = 240/497 (48%), Positives = 335/497 (67%), Gaps = 1/497 (0%)
 Frame = -1

Query: 1672 ILGHLHMLMGDELPHHVLGKLADKHGPAFLIHVGPHPELVVSSWELAKECFTTNDKCFTN 1493
            I+GHLH+L G EL H  LG +ADK+GP F I +G +  LVVS+ ++AKECFTTNDK F +
Sbjct: 34   IIGHLHLLGGSELRHKTLGAMADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFAS 93

Query: 1492 RPSNKAFKYLTYDQASVGFAPYGPLWIEMRKVSKSNLLSNQRIQMQKQVRATEVDAFFNE 1313
            RP++ A K L YD    G APYG  W+E+RK++ S LLSN+R+++ K VR +E+DA   +
Sbjct: 94   RPTSTASKILGYDYVMFGMAPYGQYWVELRKITMSELLSNRRLELLKHVRDSEIDASIQD 153

Query: 1312 LYQSCXXXXXXXXXXXXXXXDKDGTVVGRGPVIVEMNKWFEELTLNLVTRMVSGKEHVGT 1133
            LY+                         +GPV+V+M +WF +LTLN++ RM++GK + G+
Sbjct: 154  LYKVWKNHDK-----------------AKGPVLVDMKQWFGDLTLNVILRMIAGKRYSGS 196

Query: 1132 NARRGDSEAKYYKKVIDDAAHFMGNLVISDIFPSLGWLDYLLGHEGSMKKTGKELDSIFS 953
             +   ++EA+  +K + D    +G  +I D  P L W D L G++  MK T KELDS+F 
Sbjct: 197  MSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWFD-LQGYKKEMKNTAKELDSVFQ 255

Query: 952  SWVKEHQQKRKVQGSSNKDPEEDFIDITLSAIEESQLHGLDTETFIKSICVGMILGGSDT 773
             W++EH + R+  G  N+  E+DF+D+ +S +E++++   D +T IKS C+ ++ GG DT
Sbjct: 256  RWLEEHNRMRQT-GELNR--EQDFMDVLMSILEDTRISEYDNDTIIKSTCLSIVTGGGDT 312

Query: 772  TSVAXXXXXXXXLNNRDILEKAQDELDQHVGKERKVDDSDINNLVYLGAIVKESMRLYQV 593
            T V         LNN+  L+KAQDELD HVGK+R+V++SDI NLVYL AI KE++RLY  
Sbjct: 313  TMVTLTWILSLLLNNKHALKKAQDELDSHVGKDRQVEESDIKNLVYLQAITKEALRLYPA 372

Query: 592  GPLI-EREATEDCNIGGFHVKKGSRLMVNIWKVQQDPTVWSDPSEFRPERFLSGNLGMDV 416
            GPL   R A  DC + G+HV  G+RL+VN +K+Q+DP VWS+PSEFRPERFL+ ++ MDV
Sbjct: 373  GPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPERFLTSHVNMDV 432

Query: 415  KGQHFELIPFGSGRRMCPGLGFALNVIHLVLARLLHSFDLATPLNAKVDMTETSSVTNYK 236
            KG H+ELIPFG+GRR CPG+ F L V+ LVLAR LH FD  T ++  VDMTET+ +TN K
Sbjct: 433  KGLHYELIPFGAGRRSCPGMSFTLQVVPLVLARFLHEFDSKTEMDMPVDMTETAGLTNAK 492

Query: 235  GTPLQVLLTPRLKSTLY 185
             TPL+V++TPRL   +Y
Sbjct: 493  ATPLEVVITPRLHPEIY 509


>ref|XP_003523888.1| PREDICTED: cytochrome P450 82C4-like [Glycine max]
          Length = 526

 Score =  484 bits (1246), Expect = e-134
 Identities = 252/501 (50%), Positives = 338/501 (67%), Gaps = 5/501 (0%)
 Frame = -1

Query: 1672 ILGHLHMLMGDE-LPHHVLGKLADKHGPAFLIHVGPHPELVVSSWELAKECFTTNDKCFT 1496
            ++GHLH+L GD+ L +  LG +AD++GPAF I +G     VVSSWE+AKECFT+NDK   
Sbjct: 46   LIGHLHLLGGDDQLLYRTLGTMADQYGPAFNIWLGTRRAFVVSSWEVAKECFTSNDKALA 105

Query: 1495 NRPSNKAFKYLTYDQASVGFAPYGPLWIEMRKVSKSNLLSNQRIQMQKQVRATEVDAFFN 1316
            +RP+  A K++ Y+ A  GFAPY P W EMRK++   LLSN+R++M K V  +E++    
Sbjct: 106  SRPTTVAAKHMGYNYAVFGFAPYSPFWREMRKIATLELLSNRRLEMLKHVMVSELNMVMR 165

Query: 1315 ELYQSCXXXXXXXXXXXXXXXDKDGTVVGRGPVIVEMNKWFEELTLNLVTRMVSGKEHVG 1136
            +LY S                          PV+VE+N+W E+LTLN+V RMV+GK + G
Sbjct: 166  DLYNSWVQNRSR-------------------PVLVELNRWLEDLTLNMVVRMVAGKRYFG 206

Query: 1135 TNAR-RGDSEAKYYKKVIDDAAHFMGNLVISDIFPSLGWLDYLLGHEGSMKKTGKELDSI 959
             +A    D EA+  +K I+   H +G  V+SD  P L W D + GHE +MKKT KELD+I
Sbjct: 207  ASASCDNDDEARRCQKAINQFFHLIGIFVVSDALPFLRWFD-VQGHERAMKKTAKELDAI 265

Query: 958  FSSWVKEHQQKRKVQGSSNKDPEEDFIDITLSAIEESQLHGL--DTETFIKSICVGMILG 785
               W+KEH+++R V G    + E+DFIDI LS  +   L     D++T IKS C+ +ILG
Sbjct: 266  LEGWLKEHREQR-VDGEIKAEGEQDFIDIMLSLQKGGHLSNFQYDSDTSIKSTCLALILG 324

Query: 784  GSDTTSVAXXXXXXXXLNNRDILEKAQDELDQHVGKERKVDDSDINNLVYLGAIVKESMR 605
            GSDTT+          LNNR  L+KAQ+ELD +VG ER+V++SDI NL Y+ AI+KE++R
Sbjct: 325  GSDTTAGTVTWAISLLLNNRQALKKAQEELDLNVGMERQVEESDIRNLAYVQAIIKETLR 384

Query: 604  LYQVGPLI-EREATEDCNIGGFHVKKGSRLMVNIWKVQQDPTVWSDPSEFRPERFLSGNL 428
            LY  GPL+  REA EDCN+ G+HV  G+RL+VN+WK+ +DP VW +PS FRPERFL+ + 
Sbjct: 385  LYPAGPLLGPREAQEDCNVAGYHVPAGTRLVVNLWKIHRDPRVWQEPSAFRPERFLTSD- 443

Query: 427  GMDVKGQHFELIPFGSGRRMCPGLGFALNVIHLVLARLLHSFDLATPLNAKVDMTETSSV 248
             +DV+GQ+FELIPFGSGRR CPG+ FAL V+HL LARLLH+F+ ATP +  VDMTE+  +
Sbjct: 444  AVDVRGQNFELIPFGSGRRSCPGMSFALQVLHLTLARLLHAFEFATPSDQPVDMTESPGL 503

Query: 247  TNYKGTPLQVLLTPRLKSTLY 185
            T  K TPL+VLLTPRL + LY
Sbjct: 504  TIPKATPLEVLLTPRLPAKLY 524


>ref|XP_002510298.1| cytochrome P450, putative [Ricinus communis]
            gi|223550999|gb|EEF52485.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 523

 Score =  482 bits (1240), Expect = e-133
 Identities = 246/499 (49%), Positives = 334/499 (66%), Gaps = 3/499 (0%)
 Frame = -1

Query: 1672 ILGHLHMLMG-DELPHHVLGKLADKHGPAFLIHVGPHPELVVSSWELAKECFTTNDKCFT 1496
            I+GHLH+L G D+L H  LG LADK+GPAF I +G H  LVV+S ELAKECFT NDK   
Sbjct: 47   IIGHLHLLGGADKLLHRTLGSLADKYGPAFNIRIGSHRALVVASKELAKECFTINDKTLA 106

Query: 1495 NRPSNKAFKYLTYDQASVGFAPYGPLWIEMRKVSKSNLLSNQRIQMQKQVRATEVDAFFN 1316
            +R +  A K++ YD A  GFAPY   W EMRK+    LLSN+R++M K V+A+EVD    
Sbjct: 107  SRSTTAATKHMCYDHAVFGFAPYSSHWREMRKIVMLELLSNRRLEMVKHVQASEVDLGIR 166

Query: 1315 ELYQSCXXXXXXXXXXXXXXXDKDGTVVGRGPVIVEMNKWFEELTLNLVTRMVSGKEHVG 1136
            +LY                            PVIVE+ ++FE+LTL+++ R+V+GK + G
Sbjct: 167  KLYNLWAQNRCL-------------------PVIVELKQFFEDLTLDVIVRVVAGKRYTG 207

Query: 1135 TNARRGDSEAKYYKKVIDDAAHFMGNLVISDIFPSLGWLDYLLGHEGSMKKTGKELDSIF 956
            ++    D EA+ Y+K I    H MG  V+SD  P L WLD L GHE +MKKT K+LD++ 
Sbjct: 208  SS---DDDEARQYQKAISQFFHLMGIFVVSDALPFLRWLD-LEGHEKAMKKTAKDLDAVL 263

Query: 955  SSWVKEHQQKRKVQGSSNKDPEEDFIDITLSAIEESQLHGL--DTETFIKSICVGMILGG 782
            + W+ EH+ +R+V G    + ++DFID+ LS  E+  L G   D +T IKS C+ +I G 
Sbjct: 264  AGWLDEHR-RRRVSGEVKSEGDQDFIDVMLSLEEKGHLSGFQYDADTSIKSTCLALIAGA 322

Query: 781  SDTTSVAXXXXXXXXLNNRDILEKAQDELDQHVGKERKVDDSDINNLVYLGAIVKESMRL 602
            SDTT+          LNN+  L+ A++EL++H+G ER+VD+SD+ NLVYL A++KE++RL
Sbjct: 323  SDTTTTTLVWAISLLLNNQLALKNAKEELEKHIGTERQVDESDLKNLVYLQAVIKETLRL 382

Query: 601  YQVGPLIEREATEDCNIGGFHVKKGSRLMVNIWKVQQDPTVWSDPSEFRPERFLSGNLGM 422
            Y V PLI RE  EDC +GG+HV  G+RL+VN+WK+Q+DP +W+  S F+PERFL+ +  +
Sbjct: 383  YPVAPLIPREFVEDCRVGGYHVPAGTRLLVNVWKIQRDPMLWTKASAFQPERFLTSHADI 442

Query: 421  DVKGQHFELIPFGSGRRMCPGLGFALNVIHLVLARLLHSFDLATPLNAKVDMTETSSVTN 242
            DV+G HFEL+PFGSGRR CPG  FAL+ +HL LAR LH+FD+ATP++  VDMTE S  T 
Sbjct: 443  DVRGHHFELLPFGSGRRSCPGASFALHALHLTLARFLHAFDVATPMDQPVDMTERSGTTL 502

Query: 241  YKGTPLQVLLTPRLKSTLY 185
             K TPL+VLL+PRL + LY
Sbjct: 503  PKATPLEVLLSPRLPAKLY 521


>dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 534

 Score =  481 bits (1237), Expect = e-133
 Identities = 252/504 (50%), Positives = 335/504 (66%), Gaps = 8/504 (1%)
 Frame = -1

Query: 1672 ILGHLHMLMGDELPHHVLGKLADKHGPAFLIHVGPHPELVVSSWELAKECFTTNDKCFTN 1493
            ++GHL++L   +  + VLG+LAD++GPAF I  G HP LVVSSWEL K CFTTNDK F++
Sbjct: 54   VIGHLNLLSVPKPAYIVLGELADQYGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSS 113

Query: 1492 RPSNKAFKYLTYDQASVGFAPYGPLWIEMRKVSKSNLLSNQRIQMQKQVRATEVDAFFNE 1313
            R  NKA KY+ YDQ ++ FAP+GP W E+RK+   NLLSN+R++M K  R +E+DA   +
Sbjct: 114  RLVNKAIKYMFYDQKTISFAPHGPYWRELRKMITLNLLSNERLKMLKHQRISEMDACLKK 173

Query: 1312 LYQSCXXXXXXXXXXXXXXXDKDGTVVGRGPVIVEMNKWFEELTLNLVTRMVSGKEHVGT 1133
            LY+                  KD        V+V+M+KWF +++ N+VTR+V+GK   G 
Sbjct: 174  LYE-------------LSTKRKDENA----GVLVDMSKWFGDISFNVVTRIVAGKHIFGP 216

Query: 1132 NARRGDSEAKYYKKVIDDAAHFMGNLVISDIFPSLGWLDYLLGHEGSMKKTGKELDSIFS 953
               R       Y  V+++A   M  +V SD+ P LGWLD L G +  +K+T KELDS   
Sbjct: 217  KTER-------YMNVMEEARRLMDVMVFSDVIPYLGWLDRLRGVDSEIKRTAKELDSALE 269

Query: 952  SWVKEHQQKRK-----VQGSSNKDPEE--DFIDITLSAIEESQLHGLDTETFIKSICVGM 794
            SWV EH+QKR      + G  N   EE  DFIDI LS I +++L G D  T IK+I   M
Sbjct: 270  SWVDEHRQKRVSISAGIGGIVNITEEEEIDFIDIMLSIIAKNKLLGDDPGTLIKAIVQEM 329

Query: 793  ILGGSDTTSVAXXXXXXXXLNNRDILEKAQDELDQHVGKERKVDDSDINNLVYLGAIVKE 614
             L   D T+V         LNN+ +L++AQ ELD  VGKER+V+DSDIN L Y+ AIVKE
Sbjct: 330  YLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGKERQVEDSDINTLPYIQAIVKE 389

Query: 613  SMRLYQVGPLIEREATEDCNIGGFHVKKGSRLMVNIWKVQQDPTVW-SDPSEFRPERFLS 437
            SMRLY  GP+IERE TEDC++G F +  G+RL +N+WK+Q+DP VW +DP EF+PERFL+
Sbjct: 390  SMRLYPPGPIIERETTEDCDVGDFRIPAGTRLWINLWKLQRDPNVWPNDPQEFQPERFLN 449

Query: 436  GNLGMDVKGQHFELIPFGSGRRMCPGLGFALNVIHLVLARLLHSFDLATPLNAKVDMTET 257
            G+  +D+KGQHFELIPFGSGRRMCPG+ F+L V+HLVLAR++H F+L TP +A +DM+ T
Sbjct: 450  GHADIDMKGQHFELIPFGSGRRMCPGVSFSLQVMHLVLARIIHGFELKTPTDADIDMSTT 509

Query: 256  SSVTNYKGTPLQVLLTPRLKSTLY 185
              + ++K TPL+VLLTPR     Y
Sbjct: 510  LGMISWKATPLEVLLTPRFPPVFY 533