BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00012725
         (967 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|A2A1A1.2|NCS2_COPJA RecName: Full=S-norcoclaurine synthase 2;...   217   4e-54
sp|Q67A25.1|NCS_THLFG RecName: Full=S-norcoclaurine synthase; Fl...   215   1e-53
pdb|2VNE|A Chain A, The X-Ray Structure Of Norcoclaurine Synthas...   186   9e-45
gb|ACO90247.1| norcoclaurine synthase [Thalictrum flavum]             182   1e-43
gb|ACO90254.1| pathogenesis-related (PR)-10-related norcoclaurin...   119   1e-24

>sp|A2A1A1.2|NCS2_COPJA RecName: Full=S-norcoclaurine synthase 2; AltName:
           Full=Pathogenesis related protein 10A; Short=CjPR10A;
           Flags: Precursor gi|289176978|dbj|BAF45338.2|
           pathogenesis related protein 10A [Coptis japonica var.
           dissecta]
          Length = 196

 Score =  217 bits (552), Expect = 4e-54
 Identities = 118/194 (60%), Positives = 137/194 (70%), Gaps = 1/194 (0%)
 Frame = +1

Query: 97  VLFVLLMFLGTMNCNNLVVSGRPLLHGITNKESTVSKVLEHELKVAASADEVWSVYGSXX 276
           VL V LMF+GT+NC  L+ +GRPLLH +T KE TV  +L HEL+VAASADEVWSV GS  
Sbjct: 6   VLVVFLMFIGTINCERLIFNGRPLLHRVT-KEETV--MLYHELEVAASADEVWSVEGSPE 62

Query: 277 XXXXXXXXXX-GSFEXXXXXXXXXXXXXXXLTFPPGEFPHAYKEKFVFIDHKLRILKVQI 453
                      G F                +TFPPG+FPH Y+EKFVF DHK R   V+ 
Sbjct: 63  LGLHLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEQ 122

Query: 454 IDGDYANLGVTYYMDTIIVVETGPDSCVIKSSMEYYVKPEFAKIVEPLIDTVSVASMAEA 633
           IDGD+ +LGVTYYMDTI VV TGPDSCVIKS+ EY+VKPEFAKIV+PLIDTV +A M+EA
Sbjct: 123 IDGDFFDLGVTYYMDTIRVVATGPDSCVIKSTTEYHVKPEFAKIVKPLIDTVPLAIMSEA 182

Query: 634 VAKKVLEEKHKSSE 675
           +AK VLE KHKSSE
Sbjct: 183 IAKVVLENKHKSSE 196


>sp|Q67A25.1|NCS_THLFG RecName: Full=S-norcoclaurine synthase; Flags: Precursor
           gi|38492394|gb|AAR22502.1| (S)-norcoclaurine synthase
           [Thalictrum flavum subsp. glaucum]
           gi|226897754|gb|ACO90248.1| norcoclaurine synthase
           [Thalictrum flavum]
          Length = 210

 Score =  215 bits (548), Expect = 1e-53
 Identities = 109/197 (55%), Positives = 139/197 (70%), Gaps = 2/197 (1%)
 Frame = +1

Query: 94  EVLFVLLMFLGTMNCNNLVVSGRPLLH--GITNKESTVSKVLEHELKVAASADEVWSVYG 267
           EV+FV LM LGT+NC  L+++GRP LH  GI N+ STV+KV+ HEL+VAASAD++W+VY 
Sbjct: 5   EVVFVFLMLLGTINCQKLILTGRPFLHHQGIINQVSTVTKVIHHELEVAASADDIWTVYS 64

Query: 268 SXXXXXXXXXXXXGSFEXXXXXXXXXXXXXXXLTFPPGEFPHAYKEKFVFIDHKLRILKV 447
                        G+FE               +TF PGEFPH YKEKF+ +D++ R+ KV
Sbjct: 65  WPGLAKHLPDLLPGAFEKLEIIGDGGVGTILDMTFVPGEFPHEYKEKFILVDNEHRLKKV 124

Query: 448 QIIDGDYANLGVTYYMDTIIVVETGPDSCVIKSSMEYYVKPEFAKIVEPLIDTVSVASMA 627
           Q+I+G Y +LGVTYYMDTI VV TG DSCVIKSS EY+VKPEF KIVEPLI T  +A+MA
Sbjct: 125 QMIEGGYLDLGVTYYMDTIHVVPTGKDSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAMA 184

Query: 628 EAVAKKVLEEKHKSSEE 678
           +A++K VLE K KS+ +
Sbjct: 185 DAISKLVLEHKSKSNSD 201


>pdb|2VNE|A Chain A, The X-Ray Structure Of Norcoclaurine Synthase From
           Thalictrum Flavum gi|195927323|pdb|2VNE|B Chain B, The
           X-Ray Structure Of Norcoclaurine Synthase From
           Thalictrum Flavum gi|195927324|pdb|2VQ5|A Chain A, X-Ray
           Structure Of Norcoclaurine Synthase From Thalictrum
           Flavum In Complex With Dopamine And Hydroxybenzaldehyde
           gi|195927325|pdb|2VQ5|B Chain B, X-Ray Structure Of
           Norcoclaurine Synthase From Thalictrum Flavum In Complex
           With Dopamine And Hydroxybenzaldehyde
          Length = 201

 Score =  186 bits (471), Expect = 9e-45
 Identities = 96/180 (53%), Positives = 122/180 (67%), Gaps = 2/180 (1%)
 Frame = +1

Query: 145 LVVSGRPLLH--GITNKESTVSKVLEHELKVAASADEVWSVYGSXXXXXXXXXXXXGSFE 318
           L+++GRP LH  GI N+ STV+KV+ HEL+VAASAD++W+VY              G+FE
Sbjct: 7   LILTGRPFLHHQGIINQVSTVTKVIHHELEVAASADDIWTVYSWPGLAKHLPDLLPGAFE 66

Query: 319 XXXXXXXXXXXXXXXLTFPPGEFPHAYKEKFVFIDHKLRILKVQIIDGDYANLGVTYYMD 498
                           TF PGEFPH YKEKF+ +D++ R+ KVQ I+G Y +LGVTYY D
Sbjct: 67  KLEIIGDGGVGTILDXTFVPGEFPHEYKEKFILVDNEHRLKKVQXIEGGYLDLGVTYYXD 126

Query: 499 TIIVVETGPDSCVIKSSMEYYVKPEFAKIVEPLIDTVSVASMAEAVAKKVLEEKHKSSEE 678
           TI VV TG DSCVIKSS EY+VKPEF KIVEPLI T  +A+ A+A++K VLE K KS+ +
Sbjct: 127 TIHVVPTGKDSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAXADAISKLVLEHKSKSNSD 186


>gb|ACO90247.1| norcoclaurine synthase [Thalictrum flavum]
          Length = 190

 Score =  182 bits (462), Expect = 1e-43
 Identities = 94/175 (53%), Positives = 121/175 (69%), Gaps = 2/175 (1%)
 Frame = +1

Query: 160 RPLLH--GITNKESTVSKVLEHELKVAASADEVWSVYGSXXXXXXXXXXXXGSFEXXXXX 333
           RP L+  GI N+ STV+K + HEL+VAASAD++WSVY              G+FE     
Sbjct: 1   RPFLNRQGIINQVSTVTKGVHHELEVAASADDIWSVYSWPGLAKHLPDLLPGAFEKLEII 60

Query: 334 XXXXXXXXXXLTFPPGEFPHAYKEKFVFIDHKLRILKVQIIDGDYANLGVTYYMDTIIVV 513
                     +TF PGEFPH YKEKF+ +D++ R+ KVQ+I+G Y +LGVTYYMDTI V+
Sbjct: 61  GDGGVGTILDMTFTPGEFPHEYKEKFILVDNEHRLKKVQMIEGGYLDLGVTYYMDTIQVI 120

Query: 514 ETGPDSCVIKSSMEYYVKPEFAKIVEPLIDTVSVASMAEAVAKKVLEEKHKSSEE 678
            TG +SCVIKSS EY+VKPEF KIVEPLI T  +A+MAEA++K VLE K+KS+ +
Sbjct: 121 PTGTNSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAMAEAISKLVLEHKYKSNSD 175


>gb|ACO90254.1| pathogenesis-related (PR)-10-related norcoclaurine synthase-like
           protein [Eschscholzia californica]
          Length = 184

 Score =  119 bits (297), Expect = 1e-24
 Identities = 64/160 (40%), Positives = 91/160 (56%), Gaps = 3/160 (1%)
 Frame = +1

Query: 205 KVLEHELKVAASADEVWSVYGSXXXXXXXXXXXXGSFEXXXXXXXXXXXXXXXL--TFPP 378
           K LE++ KV ASAD++W+VY S              FE               L   +PP
Sbjct: 3   KELENQRKVEASADDIWAVYSSPDLPKLIVKLLPSVFESIDIVEGTGGSVGTVLHLVYPP 62

Query: 379 GEFPHAYKEKFVFIDHKLRILKVQIIDGDYANLGVTYYMDTIIVVETGPDSCVIKSSMEY 558
           G  PH YKEKF+ IDH+ R+ +V+ I G Y ++GVT+YMD+  ++E GP+SC+IKS   Y
Sbjct: 63  GSIPHTYKEKFITIDHEKRLKEVRQIQGGYLDMGVTFYMDSFHILENGPNSCIIKSMTTY 122

Query: 559 YV-KPEFAKIVEPLIDTVSVASMAEAVAKKVLEEKHKSSE 675
            V   E  + V P I   S+  MAEA++K VL++K  S++
Sbjct: 123 EVPNEEVGEKVSPFISIDSLVGMAEAISKYVLDKKKSSAK 162