BLASTX nr result
ID: Coptis25_contig00012497
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00012497
(1570 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN69116.1| hypothetical protein VITISV_031843 [Vitis vinifera] 120 1e-24
ref|XP_002284795.1| PREDICTED: F-box protein SKIP23-like [Vitis ... 119 2e-24
ref|XP_002300674.1| predicted protein [Populus trichocarpa] gi|2... 116 2e-23
ref|XP_002510618.1| ubiquitin-protein ligase, putative [Ricinus ... 113 2e-22
gb|ADN34130.1| ubiquitin-protein ligase [Cucumis melo subsp. melo] 102 8e-22
>emb|CAN69116.1| hypothetical protein VITISV_031843 [Vitis vinifera]
Length = 389
Score = 120 bits (301), Expect = 1e-24
Identities = 100/325 (30%), Positives = 154/325 (47%), Gaps = 52/325 (16%)
Frame = -2
Query: 1410 IRLPFNSHCI---GSSQGWLAIEEAQGIKQTSSIRLLNPFLAVNNEIRLPLLTPTPS--- 1249
++LP SH GSS GWL I + + I LLNP +++ LP L+ P+
Sbjct: 78 LQLPEASHRRRRRGSSHGWLLI-----LDEAPEIFLLNPL--TRSKVHLPPLSTFPNVVR 130
Query: 1248 -------------------------EPWSSKINKIVLSANPASTSDYVVMAIHSALSVLA 1144
+ S I K +LS++PA+ SD++ + I + LA
Sbjct: 131 FNFADVGREYALRTSSGDVYTRSLKKMRDSFIKKTILSSSPANGSDFIAVVILNQTGDLA 190
Query: 1143 FWRFGDEAWTRINCLEAF-DDIIYYKHQFYAVNSSGQVLLCDISSPHPVVRLVAESPPLT 967
R D++WT + +++ +D+I+++ FYAVN G + +CD+S P V ++ E P
Sbjct: 191 LCRKSDDSWTFLEDAQSYSEDVIFHRGLFYAVNKYGSIAVCDVSGSSPRVSII-EMPQQF 249
Query: 966 GPNAKNYLVELSGNLLCIFREVQ----------DFKWKTTGCKVFKLDPSKLVWKKLESL 817
G + + YLVE LL + R + ++TT +V +LD W+ + SL
Sbjct: 250 GGDLQ-YLVESGEELLLVTRYLDLTYDVEXDQTSLIYRTTRFQVCRLDSKAPRWEVVSSL 308
Query: 816 CGHILFLGGISSMSVSTSHFPGCCKSNCIYFGYDACSE----LGWKASCFDTVVFHLEDG 649
LFLG S+S+S+ FPG CK NCIY+ D + +G + D +F+LEDG
Sbjct: 309 GDRALFLGENLSLSLSSVDFPG-CKGNCIYYTDDYSEDNYDGIGGEQ---DLGIFNLEDG 364
Query: 648 SFERLVPPMCKH------SPLWIEP 592
S E P C H PLW+ P
Sbjct: 365 SIE---PLPCSHFRIRWPPPLWVTP 386
>ref|XP_002284795.1| PREDICTED: F-box protein SKIP23-like [Vitis vinifera]
Length = 389
Score = 119 bits (299), Expect = 2e-24
Identities = 100/325 (30%), Positives = 154/325 (47%), Gaps = 52/325 (16%)
Frame = -2
Query: 1410 IRLPFNSHCI---GSSQGWLAIEEAQGIKQTSSIRLLNPFLAVNNEIRLPLLTPTPS--- 1249
++LP SH GSS GWL I + + I LLNP +++ LP L+ P+
Sbjct: 78 LQLPEASHRRRRRGSSHGWLLI-----LDEAPEIFLLNPL--TRSKVHLPPLSTFPNVVR 130
Query: 1248 -------------------------EPWSSKINKIVLSANPASTSDYVVMAIHSALSVLA 1144
+ S I K +LS++PA+ SD++ + I + LA
Sbjct: 131 FNFADVGREYALRTSSGDVYTRSLKKMRDSFIKKTILSSSPANGSDFIAVVILNQTGDLA 190
Query: 1143 FWRFGDEAWTRINCLEAF-DDIIYYKHQFYAVNSSGQVLLCDISSPHPVVRLVAESPPLT 967
R D++WT + +++ +D+I+++ FYAVN G + +CD+S P V ++ E P
Sbjct: 191 LCRKSDDSWTFLEDAQSYAEDVIFHRGLFYAVNKYGSIAVCDVSGSSPRVSII-EMPQQF 249
Query: 966 GPNAKNYLVELSGNLLCIFR----------EVQDFKWKTTGCKVFKLDPSKLVWKKLESL 817
G + + YLVE LL + R + ++TT +V +LD W+ + SL
Sbjct: 250 GGDLQ-YLVESGEELLLVTRYLDLTYDVEPDQTSLIYRTTRFQVCRLDLKAPRWEVVSSL 308
Query: 816 CGHILFLGGISSMSVSTSHFPGCCKSNCIYFGYDACSE----LGWKASCFDTVVFHLEDG 649
LFLG S+S+S+ FPG CK NCIY+ D + +G + D +F+LEDG
Sbjct: 309 GDRALFLGENLSLSLSSVDFPG-CKGNCIYYTDDYSEDNYDGIGGEQ---DLGIFNLEDG 364
Query: 648 SFERLVPPMCKH------SPLWIEP 592
S E P C H PLW+ P
Sbjct: 365 SIE---PLPCSHFRIRWPPPLWVTP 386
>ref|XP_002300674.1| predicted protein [Populus trichocarpa] gi|222842400|gb|EEE79947.1|
predicted protein [Populus trichocarpa]
Length = 393
Score = 116 bits (290), Expect = 2e-23
Identities = 100/327 (30%), Positives = 152/327 (46%), Gaps = 49/327 (14%)
Frame = -2
Query: 1425 YDLEEIRLPFNSHC---IGSSQGWLAIEEAQGIKQTSSIRLLNPFLAVNNEIRLPLLTPT 1255
Y + LP SH GSS GWL I + + SI ++NP +I LP ++
Sbjct: 75 YKFHSLNLPEASHRKKHCGSSHGWLII-----LDDSPSILVINPL--TRRKIHLPAISSF 127
Query: 1254 PS----------------EPWSSK------------INKIVLSANPASTSD--YVVMAIH 1165
P+ P + I K+V+S++P +V MAI
Sbjct: 128 PNVVSFDYANVGREYLLVNPQGERNTASLRQMRDGFIKKVVMSSSPLEKKGDFFVAMAIL 187
Query: 1164 SALSVLAFWRFGDEAWTRINCLEAF-DDIIYYKHQFYAVNSSGQVLLCDISSPHPVVRLV 988
+ LA+ + GDE W + +F +D+IY+ FYAVN +GQ+++CD++ P V +
Sbjct: 188 CSTGDLAYCKNGDECWNIVENSRSFSEDVIYFNGLFYAVNKAGQIVVCDVNGNSPKVSFI 247
Query: 987 AESPPLTGPNAKNYLVELSGNLLCIFR------EVQDFKWKTTGCKVFKLDPSKLVWKKL 826
E+P G + + YLV LL + R E F +KT +VFKLD + W+++
Sbjct: 248 -ETPRQIGGDMQ-YLVSSGDGLLLVTRYLDLDVEFDIFIYKTARFEVFKLDLNGPRWERV 305
Query: 825 ESLCGHILFLGGISSMSVSTSHFPGCCKSNCIYFGYD-ACSELGWKASCFDTVVFHLEDG 649
SL +LF+G SS+++S S G C +CIY+ D + + D +F L DG
Sbjct: 306 TSLGDMMLFIGENSSLALSASGLSG-CMGDCIYYTDDYSANNYDGHIGEHDLGIFKLSDG 364
Query: 648 SFERLVPPMCKHS--------PLWIEP 592
S E L P ++S PLWI P
Sbjct: 365 SIEPL-PCYPRNSYSRLQWPPPLWISP 390
>ref|XP_002510618.1| ubiquitin-protein ligase, putative [Ricinus communis]
gi|223551319|gb|EEF52805.1| ubiquitin-protein ligase,
putative [Ricinus communis]
Length = 398
Score = 113 bits (282), Expect = 2e-22
Identities = 94/315 (29%), Positives = 147/315 (46%), Gaps = 49/315 (15%)
Frame = -2
Query: 1389 HCIGSSQGWLAIEEAQGIKQTSSIRLLNPFLAVNNEIRLPLLTPTPS------------- 1249
HC GSS GWL I + T +I L+NP ++ LP L+ P+
Sbjct: 92 HC-GSSHGWLMI-----LDDTPTILLINPLTRA--KLSLPPLSSFPNVISFNYSDIGREY 143
Query: 1248 ---------------EPWSSKINKIVLSANPASTSDYVVMAIHSALSVLAFWRFGDEAWT 1114
+ S I K+VLS++P +++VV+AI + LA+ R GD++W+
Sbjct: 144 ALRNLLGERVTVSLRQMRDSFIRKVVLSSSPLKDNNFVVIAILNQTDDLAYCRNGDKSWS 203
Query: 1113 RINCLEAF-DDIIYYKHQFYAVNSSGQVLLCDISSPHPVVRLVAESPPLTGPNAKNYLVE 937
+ +F +D+I+ FYAVN +GQ+++CDISS P V + E+P G + + YLV
Sbjct: 204 LVENARSFCEDVIFVNGMFYAVNKAGQIVICDISSDSPRVSFM-ETPRQAGGDMQ-YLVN 261
Query: 936 LSGNLLCIFREVQ------------DFKWKTTGCKVFKLDPSKLVWKKLESLCGHILFLG 793
LL + R + ++T +VF+LD + W ++ +L LF+G
Sbjct: 262 SGDELLLVTRYLDLEYEFDHPDMEPHLIYRTIRFEVFRLDWNGPQWLRMSTLGDKALFIG 321
Query: 792 GISSMSVSTSHFPGCCKSNCIYFGYDAC-SELGWKASCFDTVVFHLEDGSFERL------ 634
SS+S+S + F G C NCIY+ D + D +F L D + E L
Sbjct: 322 ENSSLSLSATDFSG-CMGNCIYYTDDYLDANYDGHFGDHDLGIFKLCDETIEPLPCYPRN 380
Query: 633 -VPPMCKHSPLWIEP 592
P+ PLW+ P
Sbjct: 381 FTSPLQWSPPLWVSP 395
>gb|ADN34130.1| ubiquitin-protein ligase [Cucumis melo subsp. melo]
Length = 392
Score = 102 bits (255), Expect(2) = 8e-22
Identities = 97/317 (30%), Positives = 143/317 (45%), Gaps = 54/317 (17%)
Frame = -2
Query: 1380 GSSQGWLAIEEAQGIKQTSSIRLLNPFLAVNNEIRLPLLTPTPS---------------- 1249
GSS GWL I + +T SI LLNP + RLP L+ P+
Sbjct: 89 GSSHGWLTI-----LDETPSILLLNPLTRA--KFRLPPLSTFPNVVSFDYSRVGREYLIR 141
Query: 1248 ------------EPWSSKINKIVLSANPASTSDYVVMAIHSALSVLAFWRFGDEAWTRIN 1105
+ S + KIVLS++P++ +D++ +AI + LAF R G +WT ++
Sbjct: 142 TPTGHIYTRNLRQMRDSFVKKIVLSSSPSNQNDFLAVAILNHSGDLAFCRSGGGSWTFVD 201
Query: 1104 CLEA-FDDIIYYKHQFYAVNSSGQVLLCDISSPHPVVRLVAESPPLTGPNAKNYLVELSG 928
+ +D+IY FYAV+ G V L D+ V LVA L G YLV L
Sbjct: 202 DAPSDCEDVIYTDGVFYAVDKYGVVSLMDLRGSRSQVSLVATERQLAGD--IQYLVRLGQ 259
Query: 927 NLLCIFR-----------EVQDFKWKTTGCKVFKLDPSKLVWKKLESLCGHILFLGGISS 781
LL + R E+ ++T +VF+++ W+K+E+L LF+GG SS
Sbjct: 260 ELLLVSRYLDIVNDGMDDELISVMYRTVRFEVFRMEWEGPRWEKVENLNEMALFVGGNSS 319
Query: 780 MSVSTSHFPGCCKSNCIYFGYD-ACSELGWKASCFDTVVFHLEDGSFE------------ 640
M+ S + F G NCIY+ D + S+ + D +F L D SFE
Sbjct: 320 MAFSAADF-GEISGNCIYYTDDYSDSDYQGQGEEPDMGIFRLCDESFEPLPYYSGGSHSR 378
Query: 639 -RLVPPMCKHSPLWIEP 592
RL+P P+W+ P
Sbjct: 379 RRLLP------PIWVTP 389
Score = 28.9 bits (63), Expect(2) = 8e-22
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -3
Query: 1526 HCLPPQLPMLLIPNHKKDKLSLYN 1455
H LPPQLP L+IP + + L+N
Sbjct: 44 HRLPPQLPCLIIPLYHDCRCGLFN 67