BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00012463
         (1920 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278560.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   455   e-175
ref|XP_002533700.1| amsh, putative [Ricinus communis] gi|2235263...   441   e-174
ref|XP_002321479.1| predicted protein [Populus trichocarpa] gi|2...   423   e-167
ref|XP_003528952.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   404   e-161
ref|XP_003525234.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   401   e-160

>ref|XP_002278560.1| PREDICTED: AMSH-like ubiquitin thiolesterase 1 [Vitis vinifera]
            gi|297734223|emb|CBI15470.3| unnamed protein product
            [Vitis vinifera]
          Length = 506

 Score =  455 bits (1171), Expect(2) = e-175
 Identities = 246/426 (57%), Positives = 305/426 (71%), Gaps = 4/426 (0%)
 Frame = +2

Query: 83   MKTSNGGFSIAANVPRRDVDNRISLRYYYRIADNLLRQANIFREEKNVLDLFVMLMRFSS 262
            M+ S+GG +IAA+  + DVDNRISLRYYYRIADN+L+QA+IFREEKN++DL++ML+RFSS
Sbjct: 1    MRPSSGGINIAASAQKLDVDNRISLRYYYRIADNILKQADIFREEKNIIDLYIMLLRFSS 60

Query: 263  LITETIPCHKDYKVYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKINGLATTQVDRWG 442
            L +ETIPCH+DYK   Q +K+                 P V+++ D++N   T QV+RWG
Sbjct: 61   LTSETIPCHRDYKASLQSKKNYLKKNLLRALCELEELKPAVRQKIDELNQKHTYQVNRWG 120

Query: 443  HHQSNYRDSSLEWPPVKKQLPKYNEIRKTPRYVGS--EGTGSLVQQDKFSLSKPMEEQYR 616
                   DSSLEWP V+KQ    +E+ KTPR      E  GS +QQ  FS +KP+EEQ+R
Sbjct: 121  ------ADSSLEWPHVRKQTLTKHEMTKTPRPTARDFEYQGSKIQQ--FSRAKPVEEQFR 172

Query: 617  KVNLNIPRPKEETLSRHSILGPNGLRGQWQPPTSNIGARYPSNIDLTPIEIPSLQKPVED 796
            ++++ I RP+EETLSRHSILGPNGL GQWQPPT +   +YPSN+DLTP++ PSLQ  VED
Sbjct: 173  RLSVGILRPREETLSRHSILGPNGLHGQWQPPTIDKRVQYPSNLDLTPVQFPSLQHHVED 232

Query: 797  NVFIQKD-TSSDLERSTLESVLSLNVDGHSLLTEELCSAPTSSLDTGLMELDMQTSIIRQ 973
             +    + +SS+ E+S+LESVL+LN D      EEL      SL +        T IIRQ
Sbjct: 233  GLMNNNEASSSEHEQSSLESVLTLNEDNQIHPAEEL-----GSLISFETTETPHTEIIRQ 287

Query: 974  PSPSPVLAEVQDLMPVTSPQVSNSVSEFAT-LPDELARSKCPLQLHISTTMMEHFLSLAK 1150
            PSP PVLA+VQDL+P T  QVS    +  T   D L  S+ PL++HIST +ME FL LAK
Sbjct: 288  PSPPPVLADVQDLIPETPTQVSEVNGQMETPSTDGLVCSEAPLEMHISTVLMESFLKLAK 347

Query: 1151 SNTDKNLETCGILAGSLKNRKFFITALIIPKQESTSDSCQATNEEEIFDVQDKQSLFQLG 1330
            SNT +NLETCGILAGSLKNRKF++TALIIPKQESTSDSCQ TNEEEIF+VQDKQSLF LG
Sbjct: 348  SNTVRNLETCGILAGSLKNRKFYVTALIIPKQESTSDSCQTTNEEEIFEVQDKQSLFPLG 407

Query: 1331 WIHTHP 1348
            WIHTHP
Sbjct: 408  WIHTHP 413



 Score =  187 bits (475), Expect(2) = e-175
 Identities = 81/99 (81%), Positives = 92/99 (92%)
 Frame = +3

Query: 1413 WIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVMAPKDSSRTHGIFRLTSPGGVSVIRN 1592
            WIHTHP+QSCFMSS+DLHTHYSYQ MLPEA+AIVMAPKD+S+ HGIFRLT+PGG+SVIR+
Sbjct: 408  WIHTHPTQSCFMSSVDLHTHYSYQTMLPEAIAIVMAPKDASKKHGIFRLTTPGGMSVIRH 467

Query: 1593 CDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDVIDLR 1709
            CD+RGFHPH  P DGGPIY+ CTDVYMNPN+ FDVIDLR
Sbjct: 468  CDQRGFHPHHSPSDGGPIYKSCTDVYMNPNIHFDVIDLR 506


>ref|XP_002533700.1| amsh, putative [Ricinus communis] gi|223526395|gb|EEF28683.1| amsh,
            putative [Ricinus communis]
          Length = 514

 Score =  441 bits (1135), Expect(2) = e-174
 Identities = 231/423 (54%), Positives = 304/423 (71%), Gaps = 6/423 (1%)
 Frame = +2

Query: 98   GGFSIAANVPRRDVDNRISLRYYYRIADNLLRQANIFREEKNVLDLFVMLMRFSSLITET 277
            G  +IA +  + DVDNRISLR+YYRIADN+L+QA+IFREEKN++DL++ML+RFSSL++ET
Sbjct: 8    GRINIATSAQKIDVDNRISLRFYYRIADNILKQADIFREEKNIIDLYIMLLRFSSLVSET 67

Query: 278  IPCHKDYKVYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKINGLATTQVDRWGHHQSN 457
            IPCH+DY+   Q +K                  P VQ++ +++N   T QV+ WG    N
Sbjct: 68   IPCHRDYRTSQQSKKIYLKQKLLNAVKELEQLKPAVQQKINELNRKHTHQVNGWGSVNQN 127

Query: 458  YRDSSLEWPPVKKQLPKYNEIRKTPRYVGSEGT----GSLVQQDKFSLSKPMEEQYRKVN 625
                SLEWPPVKK+     +   T   + +       GS  QQ   S ++P+ EQ+RK++
Sbjct: 128  ---DSLEWPPVKKKTLTGYDAGATKAVIPAAREFVYHGSRTQQ--LSYARPVGEQFRKMS 182

Query: 626  LNIPRPKEETLSRHSILGPNGLRGQWQPPTSNIGARYPSNIDLTPIEIPSLQKPVEDNVF 805
            LN P+PKEETLSRHSILGPNGL+GQWQPP S+ G  YPSNIDL+P+EIP     +E+ + 
Sbjct: 183  LNFPQPKEETLSRHSILGPNGLQGQWQPPKSDKGVWYPSNIDLSPVEIPRFDSSLENGLA 242

Query: 806  IQKDTSS-DLERSTLESVLSLNVDGHSLLTEELCSAPTSSLDTGLMELDMQTSIIRQPSP 982
            ++ D+SS +LE  + +SVL++N +  +   EEL  +P  S +T   E  +QT +IRQPSP
Sbjct: 243  LKLDSSSSELETLSSQSVLTVNDNSQTSRVEEL--SPMISFET--TETPVQTDLIRQPSP 298

Query: 983  SPVLAEVQDLMPVTSPQVSNSVSEF-ATLPDELARSKCPLQLHISTTMMEHFLSLAKSNT 1159
              VLAEVQDL+P  SPQ + + ++   + PD++ RS+ PLQLHISTTMME+F+ LAK+NT
Sbjct: 299  PAVLAEVQDLIPAMSPQATEAENKMDISSPDDIVRSESPLQLHISTTMMENFMKLAKANT 358

Query: 1160 DKNLETCGILAGSLKNRKFFITALIIPKQESTSDSCQATNEEEIFDVQDKQSLFQLGWIH 1339
            D+NLETCG+LAGSLKNRKF++TALIIPKQESTSDSCQ TNEEEIF+VQDK+SLF LGWIH
Sbjct: 359  DRNLETCGVLAGSLKNRKFYVTALIIPKQESTSDSCQTTNEEEIFEVQDKRSLFPLGWIH 418

Query: 1340 THP 1348
            THP
Sbjct: 419  THP 421



 Score =  199 bits (505), Expect(2) = e-174
 Identities = 87/99 (87%), Positives = 97/99 (97%)
 Frame = +3

Query: 1413 WIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVMAPKDSSRTHGIFRLTSPGGVSVIRN 1592
            WIHTHP+QSCFMSSID+HTHYSYQIMLPE+VAIVMAP+D+SRTHGIFRLT+PGG+SVIRN
Sbjct: 416  WIHTHPTQSCFMSSIDVHTHYSYQIMLPESVAIVMAPRDTSRTHGIFRLTTPGGMSVIRN 475

Query: 1593 CDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDVIDLR 1709
            C +RGFHPHD+PPDGGPIY+ CTDVYMNPNLKFDVIDLR
Sbjct: 476  CQQRGFHPHDQPPDGGPIYKTCTDVYMNPNLKFDVIDLR 514


>ref|XP_002321479.1| predicted protein [Populus trichocarpa] gi|222868475|gb|EEF05606.1|
            predicted protein [Populus trichocarpa]
          Length = 497

 Score =  423 bits (1088), Expect(2) = e-167
 Identities = 225/416 (54%), Positives = 293/416 (70%), Gaps = 2/416 (0%)
 Frame = +2

Query: 107  SIAANVPRRDVDNRISLRYYYRIADNLLRQANIFREEKNVLDLFVMLMRFSSLITETIPC 286
            +I A   R DVDNR +L  Y+RIA+N+L+QA IFR EKN++DL+VML+RFSSL++ETIPC
Sbjct: 5    NIEARTQRLDVDNRFALNIYFRIANNILKQAEIFRAEKNIIDLYVMLLRFSSLVSETIPC 64

Query: 287  HKDYKVYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKINGLATTQVDRWGHHQSNYRD 466
            H+ Y  + Q +KD                   VQ+R +++N   T QV+ WG++  N   
Sbjct: 65   HRGYGAFPQSKKDYLRKKLLNALGELEQLKTAVQQRINELNRKHTHQVNGWGYNSQN--- 121

Query: 467  SSLEWPPVKKQLPKYNEIRKTPRYVGSEGTGSLVQQDKFSLSKPMEEQYRKVNLNIPRPK 646
              LE PP  K++   NE         S   GS  +Q  +S  +P+E+Q+R+V+LN  RP 
Sbjct: 122  DLLEKPPYNKKILNGNE---------SLYQGSRTRQ--YSYVRPVEQQFRRVSLNFQRPN 170

Query: 647  EETLSRHSILGPNGLRGQWQPPTSNIGARYPSNIDLTPIEIPSLQKPVEDNVFIQKD-TS 823
            EETLSRHSILGPNGL  +WQPP +N G +YPS IDLTP+EIP LQ+ V+  +F++ + +S
Sbjct: 171  EETLSRHSILGPNGLNAKWQPPRTNEGVKYPSIIDLTPVEIPRLQESVKAELFVKSEHSS 230

Query: 824  SDLERSTLESVLSLNVDGHSLLTEELCSAPTSSLDTGLMELDMQTSIIRQPSPSPVLAEV 1003
            S+  RS+LES+LS+  D      EE CS    S +T  +E  +  ++IRQPSP PVLAEV
Sbjct: 231  SEPGRSSLESILSVQDDNQKCRDEEPCSL--ISFET--IETPVLPAVIRQPSPPPVLAEV 286

Query: 1004 QDLMPVTSPQVSNSVSEF-ATLPDELARSKCPLQLHISTTMMEHFLSLAKSNTDKNLETC 1180
            QDL+P T PQVS + ++   + P++L  S+ PLQLHISTT+ME+F+ +AKSNTDKNLETC
Sbjct: 287  QDLIPATPPQVSEAENKMDISSPNDLICSEAPLQLHISTTLMENFMKMAKSNTDKNLETC 346

Query: 1181 GILAGSLKNRKFFITALIIPKQESTSDSCQATNEEEIFDVQDKQSLFQLGWIHTHP 1348
            G+LAGSLKNRKF++TALIIPKQESTSDSCQ TNEEEIF+VQDKQSLF LGWIHTHP
Sbjct: 347  GVLAGSLKNRKFYVTALIIPKQESTSDSCQTTNEEEIFEVQDKQSLFPLGWIHTHP 402



 Score =  192 bits (488), Expect(2) = e-167
 Identities = 88/101 (87%), Positives = 94/101 (93%), Gaps = 2/101 (1%)
 Frame = +3

Query: 1413 WIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVMAPKDSSR--THGIFRLTSPGGVSVI 1586
            WIHTHP+QSCFMSSID+HTHYSYQIMLPEAVAIVMAP+DSSR  THGIFRLT+PGG+SVI
Sbjct: 397  WIHTHPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPRDSSRQVTHGIFRLTTPGGMSVI 456

Query: 1587 RNCDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDVIDLR 1709
            R C  RGFHPHD PPDGGPIY+ CTDVYMNPNLKFDVIDLR
Sbjct: 457  RQCQHRGFHPHDPPPDGGPIYKPCTDVYMNPNLKFDVIDLR 497


>ref|XP_003528952.1| PREDICTED: AMSH-like ubiquitin thiolesterase 1-like [Glycine max]
          Length = 519

 Score =  404 bits (1037), Expect(2) = e-161
 Identities = 218/429 (50%), Positives = 285/429 (66%), Gaps = 5/429 (1%)
 Frame = +2

Query: 77   REMKTSNGGFSIAANVPRRDVDNRISLRYYYRIADNLLRQANIFREEKNVLDLFVMLMRF 256
            R   +     +IAA+  + DVDNRISLR+YYRIADN+LRQA+IFR EKN++DL+VML+RF
Sbjct: 2    RSSSSDRNRINIAASAQKVDVDNRISLRFYYRIADNILRQADIFRAEKNIIDLYVMLLRF 61

Query: 257  SSLITETIPCHKDYKVYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKINGLATTQVDR 436
            SSL++ETIP H+DY+    R+K+                 PVVQ++ +++N     Q + 
Sbjct: 62   SSLVSETIPRHRDYRSSPPRQKESLKKKLLISLNELENLKPVVQQKINELNSKLAYQQNG 121

Query: 437  WGHHQSNYRDSSLEWPPVKKQLPKYNEIRKTPRYVGSEGTGSLVQQDKFSLSKPMEEQYR 616
             G   SN   +SL++ PVKKQ      + K  R    E      +   FS  +P+EE  R
Sbjct: 122  QGKFSSN---NSLDFSPVKKQTSASYGLIKAVRPTAGEFVYQGSRSQPFSYVRPVEEHAR 178

Query: 617  KVNLNIPRPKEETLSRHSILGPNGLRGQWQPPTSNIGARYPSNIDLTPIEIPSLQKPVED 796
            +++L +P PKEETLSRHSILGPNGL+GQW+PP  + G +YPSNIDL+P+E+PSLQ  +ED
Sbjct: 179  RLSLTLPPPKEETLSRHSILGPNGLKGQWRPPIIDKGIKYPSNIDLSPVELPSLQHSLED 238

Query: 797  NVFIQKDTS-SDLERSTLESVLSLNVD--GHSLLTEELCSAPTSSLDTGLMELDMQTSII 967
                +KD S ++  +S L+S+L+ + D   H     +    P S +     E   +  +I
Sbjct: 239  ESLKKKDNSIAEHHKSELDSILTQSEDCQPHPQPQPQHDQEPPSLISFETTETSARIEVI 298

Query: 968  RQPSPSPVLAEVQDLMPVTSPQVSNSVSEFATLP--DELARSKCPLQLHISTTMMEHFLS 1141
            RQPSP PVLAEVQDL+P  SP V+ +  +   +P  D     + P+QLHIST +ME F+ 
Sbjct: 299  RQPSPPPVLAEVQDLVPAVSPCVNEAGCK-TEIPSSDSSVHVEAPMQLHISTALMESFMK 357

Query: 1142 LAKSNTDKNLETCGILAGSLKNRKFFITALIIPKQESTSDSCQATNEEEIFDVQDKQSLF 1321
            LAKSNT KNLETCG+LAG LKNRKF+ITALIIPKQESTSDSCQ TNEEEIF+VQDK+SLF
Sbjct: 358  LAKSNTKKNLETCGVLAGLLKNRKFYITALIIPKQESTSDSCQTTNEEEIFEVQDKRSLF 417

Query: 1322 QLGWIHTHP 1348
             LGWIHTHP
Sbjct: 418  PLGWIHTHP 426



 Score =  192 bits (488), Expect(2) = e-161
 Identities = 84/99 (84%), Positives = 95/99 (95%)
 Frame = +3

Query: 1413 WIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVMAPKDSSRTHGIFRLTSPGGVSVIRN 1592
            WIHTHP+QSCFMSSIDLHTHYSYQIMLPE+VAIVMAP+DSSR HGIFRLT+PGG+SVI+ 
Sbjct: 421  WIHTHPTQSCFMSSIDLHTHYSYQIMLPESVAIVMAPRDSSRNHGIFRLTAPGGMSVIKQ 480

Query: 1593 CDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDVIDLR 1709
            CD+RGFHPH +PPDGGPIY+ CTDVYMNP+LKF+VIDLR
Sbjct: 481  CDQRGFHPHSQPPDGGPIYKTCTDVYMNPDLKFEVIDLR 519


>ref|XP_003525234.1| PREDICTED: AMSH-like ubiquitin thiolesterase 1-like [Glycine max]
          Length = 509

 Score =  401 bits (1031), Expect(2) = e-160
 Identities = 222/427 (51%), Positives = 288/427 (67%), Gaps = 8/427 (1%)
 Frame = +2

Query: 92   SNGGFSIAANVPRRDVDNRISLRYYYRIADNLLRQANIFREEKNVLDLFVMLMRFSSLIT 271
            S+   +IAA+  + DVDNRI+LR+YYRIADN+L+QA+IFR E N++DL++ML+RFSSL++
Sbjct: 3    SSETINIAASTQKLDVDNRIALRFYYRIADNILKQADIFRAETNIVDLYIMLLRFSSLVS 62

Query: 272  ETIPCHKDYKVYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKINGLATTQVDRWGH-H 448
            ETIP H+DY+   Q +K+                 P VQ++ ++ N     Q + W   H
Sbjct: 63   ETIPRHRDYRSSPQGKKEALRKKLLHSMNELEKLKPKVQQKINEFNSRRAYQPNGWEKCH 122

Query: 449  QSNYRDSSLEWPPVKKQ-LPKYNEIRKTPRYVGSEGTGSLVQQ----DKFSLSKPMEEQY 613
             +N+ D S    P KKQ L  YN+ +          TG  V Q     +FS  +P+EE  
Sbjct: 123  SNNFMDFS----PAKKQTLTSYNKKKAV-----IPTTGEFVYQGSRGQQFSSVRPVEENM 173

Query: 614  RKVNLNIPRPKEETLSRHSILGPNGLRGQWQPPTSNIGARYPSNIDLTPIEIPSLQKPVE 793
            R+++L++ RPKEETLSRHSILGPNGL+GQW+PP S+ G RYP+ IDL+P+EIPSLQ+P+ 
Sbjct: 174  RRLSLSLLRPKEETLSRHSILGPNGLKGQWRPPASDKGVRYPTIIDLSPVEIPSLQQPLV 233

Query: 794  DNVFIQKDTS-SDLERSTLESVLSLNVDGHSLLTEELCSAPTSSLDTGLMELDMQTSIIR 970
            D    +KD S S+  +S LES+L+ + D  +   +E   AP S +     E+  Q  + R
Sbjct: 234  DGSLTKKDNSISEQHKSDLESILTQSDDCKAKHADE---AP-SLISFEATEIPAQIEVTR 289

Query: 971  QPSPSPVLAEVQDLMPVTSPQV-SNSVSEFATLPDELARSKCPLQLHISTTMMEHFLSLA 1147
            QPSP PVLAEVQDL+P   P V          + D + R++ PLQLHIST+MME F+ LA
Sbjct: 290  QPSPPPVLAEVQDLVPAMLPHVIEEGCKTEIPMSDSIVRAESPLQLHISTSMMESFMKLA 349

Query: 1148 KSNTDKNLETCGILAGSLKNRKFFITALIIPKQESTSDSCQATNEEEIFDVQDKQSLFQL 1327
            KSNTDKNLETCGILAG LKNRKF+IT LIIPKQE+TS SCQATNEEEIF+VQDKQSLF L
Sbjct: 350  KSNTDKNLETCGILAGLLKNRKFYITTLIIPKQEATSSSCQATNEEEIFEVQDKQSLFPL 409

Query: 1328 GWIHTHP 1348
            GWIHTHP
Sbjct: 410  GWIHTHP 416



 Score =  191 bits (486), Expect(2) = e-160
 Identities = 85/99 (85%), Positives = 93/99 (93%)
 Frame = +3

Query: 1413 WIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVMAPKDSSRTHGIFRLTSPGGVSVIRN 1592
            WIHTHP+QSCFMSSID+HTHYSYQIMLPEAVAIVMAP DSSR HGIFRLT+PGG+SVIR 
Sbjct: 411  WIHTHPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPTDSSRNHGIFRLTTPGGMSVIRQ 470

Query: 1593 CDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDVIDLR 1709
            C +RGFHPH++PPDGGPIY  CTDVYMNP+LKFDVIDLR
Sbjct: 471  CQQRGFHPHNQPPDGGPIYDTCTDVYMNPDLKFDVIDLR 509