BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00012438
         (848 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002275735.1| PREDICTED: tubulin-specific chaperone E [Vit...   382   e-104
ref|XP_002325942.1| tubulin folding cofactor [Populus trichocarp...   377   e-102
ref|XP_002525519.1| tubulin-specific chaperone E, putative [Rici...   364   2e-98
gb|AAM22962.1|AF486853_1 tubulin folding cofactor E [Arabidopsis...   361   1e-97
ref|NP_565017.1| tubulin folding cofactor E / Pfifferling (PFI) ...   361   1e-97

>ref|XP_002275735.1| PREDICTED: tubulin-specific chaperone E [Vitis vinifera]
           gi|297741006|emb|CBI31318.3| unnamed protein product
           [Vitis vinifera]
          Length = 541

 Score =  382 bits (982), Expect = e-104
 Identities = 184/277 (66%), Positives = 228/277 (82%)
 Frame = +3

Query: 9   SEASFRVNQRVHALNDPRRIGTVKYIGPVRGYSGTWVGVDWDSGEGKHNGSINGVTYFET 188
           S A FR+ QRVH+L DPRR+GTVKY+GPV+GYSGTWVGVDWD+G+ KH+G+++G  YF+ 
Sbjct: 9   SPAEFRLGQRVHSLGDPRRMGTVKYVGPVQGYSGTWVGVDWDNGDAKHDGALDGRRYFQA 68

Query: 189 KAEKSGSFVRGQNLSSGISFLEALELRYRSDSTKEEEDEMYVLSARNKRVAIQLLGKDEL 368
            A KSGSFVR  NLS+GIS L+AL LRYRS ++KEEE+EMYVLSA N+RV++QL+GK+++
Sbjct: 69  HAAKSGSFVRPHNLSAGISLLQALLLRYRSTTSKEEEEEMYVLSASNRRVSVQLVGKEQI 128

Query: 369 QDRLSRFEELVGVSLSLLGIRSVGPPQQISTVVPNLKELDLTANLLTEWKDVGSICEQLR 548
           +D+LSRFEEL   SLS LG+ S+G P +I +VVPNLKELDLT NLL+EWKDVG+IC QL 
Sbjct: 129 EDKLSRFEELTAASLSYLGVSSIGAPFEICSVVPNLKELDLTGNLLSEWKDVGTICVQLP 188

Query: 549 NLAVLNLSHNHLAHDINQLPSLTNIRVLVLNSCGIDWTQVETLKQSLPSVEELHLMGNKL 728
            LA LNLS+N +AHDI  LP L N+RVLVLN+ GI W +VE ++ SLP++EELHLMGN L
Sbjct: 189 GLAALNLSNNLMAHDITGLPLLMNLRVLVLNNTGIKWKEVEIIRHSLPAIEELHLMGNNL 248

Query: 729 RTIVPAFSDYVQGFDSLRVLNLEDNCFSAWDEIMKLS 839
           R I PA S  VQGFD LR+LNLEDN  + WDEI+KLS
Sbjct: 249 RAITPASSSIVQGFDYLRLLNLEDNHIAEWDEILKLS 285


>ref|XP_002325942.1| tubulin folding cofactor [Populus trichocarpa]
           gi|222862817|gb|EEF00324.1| tubulin folding cofactor
           [Populus trichocarpa]
          Length = 537

 Score =  377 bits (969), Expect = e-102
 Identities = 178/275 (64%), Positives = 226/275 (82%)
 Frame = +3

Query: 15  ASFRVNQRVHALNDPRRIGTVKYIGPVRGYSGTWVGVDWDSGEGKHNGSINGVTYFETKA 194
           ++F+++QRVH+ NDPRRIGTVKYIGPV G+ GTWVGVDWD+GE KH+GS+NGV YFE ++
Sbjct: 8   STFKLDQRVHSTNDPRRIGTVKYIGPVEGHPGTWVGVDWDNGEAKHDGSLNGVRYFEARS 67

Query: 195 EKSGSFVRGQNLSSGISFLEALELRYRSDSTKEEEDEMYVLSARNKRVAIQLLGKDELQD 374
           + SGSFVR QNL++GISF+EAL +RYR   T+E+EDEMYVLSA NKRV++QL+GK+++QD
Sbjct: 68  QLSGSFVRAQNLTAGISFIEALYIRYRDQPTQEDEDEMYVLSASNKRVSVQLVGKEKIQD 127

Query: 375 RLSRFEELVGVSLSLLGIRSVGPPQQISTVVPNLKELDLTANLLTEWKDVGSICEQLRNL 554
           +LSR EEL G SLS LG+ + G P +I  +VPNLKELDLT NLL+EWKDVG ICEQL +L
Sbjct: 128 KLSRLEELTGASLSYLGVSNPGSPNEIRNIVPNLKELDLTGNLLSEWKDVGIICEQLPSL 187

Query: 555 AVLNLSHNHLAHDINQLPSLTNIRVLVLNSCGIDWTQVETLKQSLPSVEELHLMGNKLRT 734
           A LNLS+N ++H+I  LP L +I +LVLN+ GI+WTQ+E LK  LP +EELHLMGN +  
Sbjct: 188 AALNLSNNSMSHEIVGLPLLKSIHILVLNNTGINWTQIEVLKDLLPVIEELHLMGNGINA 247

Query: 735 IVPAFSDYVQGFDSLRVLNLEDNCFSAWDEIMKLS 839
           I  A S  V GFDSLR+LNLE+NC + W+EI+KLS
Sbjct: 248 IKTASSSIVHGFDSLRLLNLEENCIAEWNEIVKLS 282


>ref|XP_002525519.1| tubulin-specific chaperone E, putative [Ricinus communis]
           gi|223535198|gb|EEF36877.1| tubulin-specific chaperone
           E, putative [Ricinus communis]
          Length = 533

 Score =  364 bits (934), Expect = 2e-98
 Identities = 172/273 (63%), Positives = 221/273 (80%)
 Frame = +3

Query: 21  FRVNQRVHALNDPRRIGTVKYIGPVRGYSGTWVGVDWDSGEGKHNGSINGVTYFETKAEK 200
           F++ QRVH+ +DPRRIG VKYIGPV GYSGTWVGVDWD+GEGKH+GS+NGV YF+ +++K
Sbjct: 7   FKLGQRVHSASDPRRIGMVKYIGPVEGYSGTWVGVDWDNGEGKHDGSVNGVRYFQARSDK 66

Query: 201 SGSFVRGQNLSSGISFLEALELRYRSDSTKEEEDEMYVLSARNKRVAIQLLGKDELQDRL 380
           SGSF+R  NL+ GIS +EAL +RY+ +STKEEEDEMYVLSA NK+V+++ +GK++++D+L
Sbjct: 67  SGSFIRVPNLNPGISLVEALHIRYKGESTKEEEDEMYVLSASNKKVSVEFVGKEKIKDKL 126

Query: 381 SRFEELVGVSLSLLGIRSVGPPQQISTVVPNLKELDLTANLLTEWKDVGSICEQLRNLAV 560
           S+F+EL   SL  LGI S G P  I  +VPNLKELDLT NLL+EWKD+G+ICEQL  LA 
Sbjct: 127 SKFDELKSASLPFLGISSPGSPTDIRNLVPNLKELDLTGNLLSEWKDIGTICEQLPALAA 186

Query: 561 LNLSHNHLAHDINQLPSLTNIRVLVLNSCGIDWTQVETLKQSLPSVEELHLMGNKLRTIV 740
           LNLS+N ++  I +L  L NI +LVLN+ GI+WTQVE L   LP++EELHLMGN + TI+
Sbjct: 187 LNLSNNLMSRTIAELSQLKNIHILVLNNTGINWTQVEVLNHLLPAIEELHLMGNGIGTIM 246

Query: 741 PAFSDYVQGFDSLRVLNLEDNCFSAWDEIMKLS 839
            + S  VQGFDSLR+LNLEDN  + W+EI+KLS
Sbjct: 247 SSSSSIVQGFDSLRLLNLEDNFIAEWNEILKLS 279


>gb|AAM22962.1|AF486853_1 tubulin folding cofactor E [Arabidopsis thaliana]
           gi|21554061|gb|AAM63142.1| unknown [Arabidopsis
           thaliana]
          Length = 531

 Score =  361 bits (927), Expect = 1e-97
 Identities = 174/280 (62%), Positives = 219/280 (78%), Gaps = 1/280 (0%)
 Frame = +3

Query: 3   EKSEASFRVNQRVHALNDPRRIGTVKYIGPVRGYSGTWVGVDWDS-GEGKHNGSINGVTY 179
           E S  SF + QRVH+LND RR+GTVKY+G V GYSGTW+GVDWD  G+GKHNGS+NGV Y
Sbjct: 4   ESSNESFIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFY 63

Query: 180 FETKAEKSGSFVRGQNLSSGISFLEALELRYRSDSTKEEEDEMYVLSARNKRVAIQLLGK 359
           F  +++ S SFVR QNLS GI+ L+ALELRYR+ STK+EEDEMYVLSA N+RV+IQLLG 
Sbjct: 64  FNGRSQSSASFVRSQNLSRGITLLQALELRYRTISTKDEEDEMYVLSAGNRRVSIQLLGG 123

Query: 360 DELQDRLSRFEELVGVSLSLLGIRSVGPPQQISTVVPNLKELDLTANLLTEWKDVGSICE 539
           D++QD+LSRFEEL   SLS LG+ S+G    + +++PNLK LDLT NL+++W+++G++CE
Sbjct: 124 DKIQDKLSRFEELTSASLSYLGVSSLGVSSDLGSILPNLKLLDLTGNLISDWEEIGALCE 183

Query: 540 QLRNLAVLNLSHNHLAHDINQLPSLTNIRVLVLNSCGIDWTQVETLKQSLPSVEELHLMG 719
           QL  L  LNLS N L+ DI  LP L NIRVLVLN+ G+ WTQVE L++SLP +EELHLMG
Sbjct: 184 QLPALTTLNLSCNSLSSDIKSLPELKNIRVLVLNNSGLSWTQVEILRRSLPGIEELHLMG 243

Query: 720 NKLRTIVPAFSDYVQGFDSLRVLNLEDNCFSAWDEIMKLS 839
           N + TI    S   Q F+SLR+LNL+DNC S W E++KLS
Sbjct: 244 NMISTITSTSSSDDQAFNSLRLLNLDDNCISDWSEVLKLS 283


>ref|NP_565017.1| tubulin folding cofactor E / Pfifferling (PFI) [Arabidopsis
           thaliana] gi|26452683|dbj|BAC43424.1| unknown protein
           [Arabidopsis thaliana] gi|27311595|gb|AAO00763.1|
           Unknown protein [Arabidopsis thaliana]
           gi|31711942|gb|AAP68327.1| At1g71440 [Arabidopsis
           thaliana] gi|332197080|gb|AEE35201.1| tubulin folding
           cofactor E / Pfifferling (PFI) [Arabidopsis thaliana]
          Length = 531

 Score =  361 bits (927), Expect = 1e-97
 Identities = 174/280 (62%), Positives = 219/280 (78%), Gaps = 1/280 (0%)
 Frame = +3

Query: 3   EKSEASFRVNQRVHALNDPRRIGTVKYIGPVRGYSGTWVGVDWDS-GEGKHNGSINGVTY 179
           E S  SF + QRVH+LND RR+GTVKY+G V GYSGTW+GVDWD  G+GKHNGS+NGV Y
Sbjct: 4   ESSNESFIIGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFY 63

Query: 180 FETKAEKSGSFVRGQNLSSGISFLEALELRYRSDSTKEEEDEMYVLSARNKRVAIQLLGK 359
           F  +++ S SFVR QNLS GI+ L+ALELRYR+ STK+EEDEMYVLSA N+RV+IQLLG 
Sbjct: 64  FNGRSQSSASFVRSQNLSRGITLLQALELRYRTISTKDEEDEMYVLSAGNRRVSIQLLGG 123

Query: 360 DELQDRLSRFEELVGVSLSLLGIRSVGPPQQISTVVPNLKELDLTANLLTEWKDVGSICE 539
           D++QD+LSRFEEL   SLS LG+ S+G    + +++PNLK LDLT NL+++W+++G++CE
Sbjct: 124 DKIQDKLSRFEELTSASLSYLGVSSLGVSSDLGSILPNLKLLDLTGNLISDWEEIGALCE 183

Query: 540 QLRNLAVLNLSHNHLAHDINQLPSLTNIRVLVLNSCGIDWTQVETLKQSLPSVEELHLMG 719
           QL  L  LNLS N L+ DI  LP L NIRVLVLN+ G+ WTQVE L++SLP +EELHLMG
Sbjct: 184 QLPALTTLNLSCNSLSSDIKSLPQLKNIRVLVLNNSGLSWTQVEILRRSLPGIEELHLMG 243

Query: 720 NKLRTIVPAFSDYVQGFDSLRVLNLEDNCFSAWDEIMKLS 839
           N + TI    S   Q F+SLR+LNL+DNC S W E++KLS
Sbjct: 244 NMISTITSTSSSDDQAFNSLRLLNLDDNCISDWSEVLKLS 283