BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00012371
         (1503 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003523478.1| PREDICTED: methylenetetrahydrofolate reducta...   848   0.0  
ref|XP_002331635.1| methylenetetrahydrofolate reductase [Populus...   846   0.0  
ref|XP_002310366.1| methylenetetrahydrofolate reductase [Populus...   845   0.0  
gb|AEL33268.1| methylenetetrahydrofolate reductase [Nicotiana ta...   845   0.0  
ref|XP_003527588.1| PREDICTED: methylenetetrahydrofolate reducta...   843   0.0  

>ref|XP_003523478.1| PREDICTED: methylenetetrahydrofolate reductase 2-like [Glycine max]
          Length = 595

 Score =  848 bits (2192), Expect = 0.0
 Identities = 393/462 (85%), Positives = 434/462 (93%)
 Frame = +3

Query: 117  NKTLFSFEFFPPKTEDGVDNLFDKMDRMVQHNPSFCDITWGAGGTTADLTLDIANKMQNM 296
            N+ +FSFEFFPPKTEDGVDNLF++MDRMV HNPSFCDITWGAGGTTADLTL+IANKMQN+
Sbjct: 15   NRVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGTTADLTLEIANKMQNI 74

Query: 297  ICVETMMHLTCTNMPVEKIDHALDAIKGNGIQNVLALRGDPPHGQDKFVQTEGGFACALD 476
            +CVETMMHLTCTNMPVEKIDHAL  IK NG+QNVLALRGDPPHGQDKFVQ EGGFACA D
Sbjct: 75   VCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQDKFVQVEGGFACARD 134

Query: 477  LVKHIRAKYGDYFGITVAGYPEAHPEVIESNGVATLESYHNDLAYLKRKVDAGADVIVTQ 656
            LV+HIRAKYGDYFGIT+AGYPEAHP+VIES+G+AT E Y NDLAYLK KVDAGAD+IVTQ
Sbjct: 135  LVQHIRAKYGDYFGITIAGYPEAHPDVIESDGLATPEGYRNDLAYLKSKVDAGADLIVTQ 194

Query: 657  LFYDTDVFLKFVNDCRQMGITCPIVPGIMPINNYKGFLRMTGFCKTRVPPEIMAALDSIK 836
            LFYDTD+FLKFVNDCRQ+GITCPIVPGIMPINNYKGF+RMTGFCKT++P +IMAAL+ IK
Sbjct: 195  LFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCKTKIPADIMAALEPIK 254

Query: 837  DDEEAVRKYGIYLGTEMCKKILVHGIKTLHLYTLNMDKSALAILQNLGLIDEAKISRTLP 1016
            D+EEAV+ YGI+LGTEMCKKIL HGIKTLHLYTLNM+KSALAIL NLGLI+E+K+SR+LP
Sbjct: 255  DNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKVSRSLP 314

Query: 1017 WRRPTNIFRLKEDVRPIFWANRPKSYLSRTIGWDQFPHGRWGDSGNPSYGALTDYQFMRP 1196
            WRRP N+FR+KEDVRPIFWANRPKSY+SRTIGWDQ+PHGRWGDS NPSYGAL+DYQFMRP
Sbjct: 315  WRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSCNPSYGALSDYQFMRP 374

Query: 1197 RSRDKKLKEEWAVSLKSVEDIHDKFMQLCLGKLKSNPWSELDGLQPETKIINEQLGSINL 1376
            R+RDKKL EEWAV LKSVEDI+++F   CLGKL+SNPWSELDGLQPETKIINEQL  IN 
Sbjct: 375  RARDKKLVEEWAVPLKSVEDIYERFRLYCLGKLRSNPWSELDGLQPETKIINEQLEKINT 434

Query: 1377 KGFLTINSQPSVNAAKSDSPSVGWGGPGGYVYQKAYVEFFCS 1502
            KGFLTINSQP+VN  KSDSP+VGWGGPGGYVYQKAYVEFFCS
Sbjct: 435  KGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCS 476


>ref|XP_002331635.1| methylenetetrahydrofolate reductase [Populus trichocarpa]
            gi|222874031|gb|EEF11162.1| methylenetetrahydrofolate
            reductase [Populus trichocarpa]
          Length = 606

 Score =  846 bits (2186), Expect = 0.0
 Identities = 394/467 (84%), Positives = 433/467 (92%)
 Frame = +3

Query: 102  EAELENKTLFSFEFFPPKTEDGVDNLFDKMDRMVQHNPSFCDITWGAGGTTADLTLDIAN 281
            ++   NK +FSFEFFPPKT+DGVDNLF++MDRMV HNPSFCDITWGAGG+TADLTLDIAN
Sbjct: 20   DSSSNNKVVFSFEFFPPKTDDGVDNLFERMDRMVSHNPSFCDITWGAGGSTADLTLDIAN 79

Query: 282  KMQNMICVETMMHLTCTNMPVEKIDHALDAIKGNGIQNVLALRGDPPHGQDKFVQTEGGF 461
            KMQN+ICVETMMHLTCTNMPVEKIDHAL+ IK NGIQNVLALRGDPPHGQDKFVQ +GG 
Sbjct: 80   KMQNIICVETMMHLTCTNMPVEKIDHALETIKSNGIQNVLALRGDPPHGQDKFVQIQGGL 139

Query: 462  ACALDLVKHIRAKYGDYFGITVAGYPEAHPEVIESNGVATLESYHNDLAYLKRKVDAGAD 641
            ACALDLVKHIR+KYGDYFGITVAGYPEAHP+VI S+G ATLE YH DLAYLK+KVDAGAD
Sbjct: 140  ACALDLVKHIRSKYGDYFGITVAGYPEAHPDVIGSDGFATLEDYHKDLAYLKQKVDAGAD 199

Query: 642  VIVTQLFYDTDVFLKFVNDCRQMGITCPIVPGIMPINNYKGFLRMTGFCKTRVPPEIMAA 821
            +IVTQLFYDTD+FLKFVNDCRQ+GI CPIVPGIMPINNYKGF+RM GFCKT++P E+ AA
Sbjct: 200  LIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPINNYKGFIRMAGFCKTKIPAEVTAA 259

Query: 822  LDSIKDDEEAVRKYGIYLGTEMCKKILVHGIKTLHLYTLNMDKSALAILQNLGLIDEAKI 1001
            L+ IKD+EEAVR YGI+LGTEMCKKIL HGIKTLHLYTLNM+KSALAIL NLGLI+E+KI
Sbjct: 260  LEPIKDNEEAVRAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKI 319

Query: 1002 SRTLPWRRPTNIFRLKEDVRPIFWANRPKSYLSRTIGWDQFPHGRWGDSGNPSYGALTDY 1181
            +R LPWR PTN+FR+KEDVRPIFWANRPKSYL+RTIGWDQ+PHGRWGDS NPSYGAL+DY
Sbjct: 320  TRPLPWRPPTNVFRVKEDVRPIFWANRPKSYLTRTIGWDQYPHGRWGDSRNPSYGALSDY 379

Query: 1182 QFMRPRSRDKKLKEEWAVSLKSVEDIHDKFMQLCLGKLKSNPWSELDGLQPETKIINEQL 1361
            QFMRPR+R KKL EEWA  LKSVEDI++KF   CLGKLKS+PWSELDGLQPETKIINEQL
Sbjct: 380  QFMRPRARGKKLLEEWAAPLKSVEDIYEKFKVYCLGKLKSSPWSELDGLQPETKIINEQL 439

Query: 1362 GSINLKGFLTINSQPSVNAAKSDSPSVGWGGPGGYVYQKAYVEFFCS 1502
            G INLKGFLTINSQP+VN  KSDSPSVGWGGPGGYVYQKAY+EFFCS
Sbjct: 440  GKINLKGFLTINSQPAVNGEKSDSPSVGWGGPGGYVYQKAYLEFFCS 486


>ref|XP_002310366.1| methylenetetrahydrofolate reductase [Populus trichocarpa]
            gi|222853269|gb|EEE90816.1| methylenetetrahydrofolate
            reductase [Populus trichocarpa]
          Length = 608

 Score =  845 bits (2184), Expect = 0.0
 Identities = 397/462 (85%), Positives = 431/462 (93%)
 Frame = +3

Query: 117  NKTLFSFEFFPPKTEDGVDNLFDKMDRMVQHNPSFCDITWGAGGTTADLTLDIANKMQNM 296
            NK +FSFEFFPPKTEDGVDNLF++MDRMV HNPSFCDITWGAGG+TADLTLDIANKMQN+
Sbjct: 28   NKVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGSTADLTLDIANKMQNI 87

Query: 297  ICVETMMHLTCTNMPVEKIDHALDAIKGNGIQNVLALRGDPPHGQDKFVQTEGGFACALD 476
            ICVETMMHLTCTNMPVEKIDHAL+ IK NGIQNVLALRGDPPHGQDKFVQ +GGFACALD
Sbjct: 88   ICVETMMHLTCTNMPVEKIDHALETIKSNGIQNVLALRGDPPHGQDKFVQIQGGFACALD 147

Query: 477  LVKHIRAKYGDYFGITVAGYPEAHPEVIESNGVATLESYHNDLAYLKRKVDAGADVIVTQ 656
            LVKHIR+KYGDYFGITVAGYPEAHP+ I S+G AT E Y  DLAYLK+KVDAGAD+IVTQ
Sbjct: 148  LVKHIRSKYGDYFGITVAGYPEAHPDAIGSDGFATPEDYQKDLAYLKQKVDAGADLIVTQ 207

Query: 657  LFYDTDVFLKFVNDCRQMGITCPIVPGIMPINNYKGFLRMTGFCKTRVPPEIMAALDSIK 836
            LFYDTD+FLKFVNDCRQ+GITCPIVPGIMPINNYKGFLRMTGFCKT++P E+ AAL+ IK
Sbjct: 208  LFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPAEVTAALEPIK 267

Query: 837  DDEEAVRKYGIYLGTEMCKKILVHGIKTLHLYTLNMDKSALAILQNLGLIDEAKISRTLP 1016
            D+EEAVR YGI+LGTEMCKKIL HGIKTLHLYTLNM+KSALAIL NLGLI+E+KI+R LP
Sbjct: 268  DNEEAVRAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMNLGLIEESKITRALP 327

Query: 1017 WRRPTNIFRLKEDVRPIFWANRPKSYLSRTIGWDQFPHGRWGDSGNPSYGALTDYQFMRP 1196
            WR PTN+FR KEDVRPIFWANRPKSYLSRTIGWDQ+PHGRWGDS NPSYGAL+DYQFMRP
Sbjct: 328  WRPPTNVFRAKEDVRPIFWANRPKSYLSRTIGWDQYPHGRWGDSHNPSYGALSDYQFMRP 387

Query: 1197 RSRDKKLKEEWAVSLKSVEDIHDKFMQLCLGKLKSNPWSELDGLQPETKIINEQLGSINL 1376
            R+R KKL EEWA  LKSVEDI++KF   CLGKLKS+PWSEL+GLQPETKIINEQLG INL
Sbjct: 388  RARGKKLFEEWASPLKSVEDIYEKFKVYCLGKLKSSPWSELEGLQPETKIINEQLGRINL 447

Query: 1377 KGFLTINSQPSVNAAKSDSPSVGWGGPGGYVYQKAYVEFFCS 1502
            KGFLTINSQP+VNA KSDSPSVGWGGPGGYVYQKAY+EFFCS
Sbjct: 448  KGFLTINSQPAVNAEKSDSPSVGWGGPGGYVYQKAYLEFFCS 489


>gb|AEL33268.1| methylenetetrahydrofolate reductase [Nicotiana tabacum]
            gi|342722659|gb|AEL33270.1| methylenetetrahydrofolate
            reductase [Nicotiana tomentosiformis]
          Length = 595

 Score =  845 bits (2182), Expect = 0.0
 Identities = 393/466 (84%), Positives = 438/466 (93%)
 Frame = +3

Query: 105  AELENKTLFSFEFFPPKTEDGVDNLFDKMDRMVQHNPSFCDITWGAGGTTADLTLDIANK 284
            A+ EN+ +FSFEFFPPKTEDGV+NLF++M+RMV HNPSFCDITWGAGG+TADLTL+I+ +
Sbjct: 11   AKDENRVVFSFEFFPPKTEDGVENLFERMERMVAHNPSFCDITWGAGGSTADLTLEISKR 70

Query: 285  MQNMICVETMMHLTCTNMPVEKIDHALDAIKGNGIQNVLALRGDPPHGQDKFVQTEGGFA 464
            MQNM+CVETMMHLTCTNMPVEKIDHALD IK NGIQNVLALRGDPPHGQDKFVQ EGGFA
Sbjct: 71   MQNMVCVETMMHLTCTNMPVEKIDHALDTIKINGIQNVLALRGDPPHGQDKFVQVEGGFA 130

Query: 465  CALDLVKHIRAKYGDYFGITVAGYPEAHPEVIESNGVATLESYHNDLAYLKRKVDAGADV 644
            CALDLVKHIRAKYGDYFGITVAGYPEAHP+VI +NG+AT E Y NDLAYLKRKVDAGAD+
Sbjct: 131  CALDLVKHIRAKYGDYFGITVAGYPEAHPDVIPANGIATQEIYENDLAYLKRKVDAGADL 190

Query: 645  IVTQLFYDTDVFLKFVNDCRQMGITCPIVPGIMPINNYKGFLRMTGFCKTRVPPEIMAAL 824
            IVTQLFYDTD+FLKFVNDCRQ+GITCPIVPGIMPINNYKGFLRMTGFCKT++P EIMAAL
Sbjct: 191  IVTQLFYDTDIFLKFVNDCRQLGITCPIVPGIMPINNYKGFLRMTGFCKTKIPEEIMAAL 250

Query: 825  DSIKDDEEAVRKYGIYLGTEMCKKILVHGIKTLHLYTLNMDKSALAILQNLGLIDEAKIS 1004
            + IKD+EEAV+ YGI+LGTEMCKKIL  GIKTLHLYTLNM+KSALAIL NLGLI+E+KIS
Sbjct: 251  EPIKDNEEAVKAYGIHLGTEMCKKILASGIKTLHLYTLNMEKSALAILMNLGLIEESKIS 310

Query: 1005 RTLPWRRPTNIFRLKEDVRPIFWANRPKSYLSRTIGWDQFPHGRWGDSGNPSYGALTDYQ 1184
            R LPWRRPTN+FR+KEDVRPIFWANRPKSY+SRT+GWD++PHGRWG++ NPSYGALTDYQ
Sbjct: 311  RPLPWRRPTNVFRVKEDVRPIFWANRPKSYISRTLGWDEYPHGRWGNAQNPSYGALTDYQ 370

Query: 1185 FMRPRSRDKKLKEEWAVSLKSVEDIHDKFMQLCLGKLKSNPWSELDGLQPETKIINEQLG 1364
            FMR RSRDKKL+EEWAV+L SV+DI+++F   CLGKL+S PWSELDGLQPETKIINE+LG
Sbjct: 371  FMRARSRDKKLQEEWAVALNSVDDIYERFKDYCLGKLRSCPWSELDGLQPETKIINEKLG 430

Query: 1365 SINLKGFLTINSQPSVNAAKSDSPSVGWGGPGGYVYQKAYVEFFCS 1502
             IN KGFLTINSQP+VNA KSDSPSVGWGGPGGYVYQKAY+EFFCS
Sbjct: 431  HINTKGFLTINSQPAVNAEKSDSPSVGWGGPGGYVYQKAYLEFFCS 476


>ref|XP_003527588.1| PREDICTED: methylenetetrahydrofolate reductase 2-like [Glycine max]
          Length = 595

 Score =  843 bits (2179), Expect = 0.0
 Identities = 391/462 (84%), Positives = 431/462 (93%)
 Frame = +3

Query: 117  NKTLFSFEFFPPKTEDGVDNLFDKMDRMVQHNPSFCDITWGAGGTTADLTLDIANKMQNM 296
            N+ +FSFEFFPPKTEDGVDNLF++MDRMV HNPSFCDITWGAGGTTADLTL+IANKMQN+
Sbjct: 15   NRVVFSFEFFPPKTEDGVDNLFERMDRMVVHNPSFCDITWGAGGTTADLTLEIANKMQNI 74

Query: 297  ICVETMMHLTCTNMPVEKIDHALDAIKGNGIQNVLALRGDPPHGQDKFVQTEGGFACALD 476
            +CVETMMHLTCTNMPVEKIDHAL  IK NG+QNVLALRGDPPHGQDKFVQ EGGFACA D
Sbjct: 75   VCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQDKFVQVEGGFACARD 134

Query: 477  LVKHIRAKYGDYFGITVAGYPEAHPEVIESNGVATLESYHNDLAYLKRKVDAGADVIVTQ 656
            LV+HIRAKYGDYFGITVAGYPEAHP+VIES+G+AT E Y NDLAYLK KVDAGAD+IVTQ
Sbjct: 135  LVQHIRAKYGDYFGITVAGYPEAHPDVIESDGLATSEGYQNDLAYLKSKVDAGADLIVTQ 194

Query: 657  LFYDTDVFLKFVNDCRQMGITCPIVPGIMPINNYKGFLRMTGFCKTRVPPEIMAALDSIK 836
            LFYDTD+FLKFVNDCRQ+GITCPIVPGIMPINNYKGF+RMTGFCKT++P +IMAAL+ IK
Sbjct: 195  LFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCKTKIPADIMAALEPIK 254

Query: 837  DDEEAVRKYGIYLGTEMCKKILVHGIKTLHLYTLNMDKSALAILQNLGLIDEAKISRTLP 1016
            D+EEAV+ YGI+LGTEMCKKIL HGI TLHLYTLNM+KSALAIL NLGLI+E K+SR+LP
Sbjct: 255  DNEEAVKAYGIHLGTEMCKKILAHGINTLHLYTLNMEKSALAILMNLGLIEETKVSRSLP 314

Query: 1017 WRRPTNIFRLKEDVRPIFWANRPKSYLSRTIGWDQFPHGRWGDSGNPSYGALTDYQFMRP 1196
            WRRP N+FR+KEDVRPIFWANRPKSY+SRTIGWDQ+PHGRWGDS NPSYGAL+DYQFMRP
Sbjct: 315  WRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSCNPSYGALSDYQFMRP 374

Query: 1197 RSRDKKLKEEWAVSLKSVEDIHDKFMQLCLGKLKSNPWSELDGLQPETKIINEQLGSINL 1376
            R+RDKKL EEWAV LKS+EDI+++F   CLGKL+SNPWSELDGLQPETKIINE L  IN 
Sbjct: 375  RARDKKLVEEWAVPLKSIEDIYERFRLYCLGKLRSNPWSELDGLQPETKIINELLEKINT 434

Query: 1377 KGFLTINSQPSVNAAKSDSPSVGWGGPGGYVYQKAYVEFFCS 1502
            KGFLTINSQP+VN  KSDSP+VGWGGPGGYVYQKAYVEFFCS
Sbjct: 435  KGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCS 476