BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00011782
         (1755 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]             441   e-121
gb|ADZ55289.1| ent-kaurene synthase [Triticum aestivum]               220   e-110
gb|AEZ55689.1| kaurene synthase 2, partial [Salvia miltiorrhiza]      221   e-110
ref|XP_002525841.1| Ent-kaurene synthase B, chloroplast precurso...   213   e-109
ref|XP_002525836.1| Ent-kaurene synthase B, chloroplast precurso...   205   e-106

>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
          Length = 784

 Score =  441 bits (1133), Expect = e-121
 Identities = 255/542 (47%), Positives = 326/542 (60%)
 Frame = -2

Query: 1754 VLDETYRYWLQRDDEIFLDIETCALAFRLLRINGYAVSSDTLAEFNEEIDFFTSAGGDLK 1575
            VLDETYR WLQ ++EIFLD  TCA+AFR+LR+NG+ +SSD   + +E+  F +S GG +K
Sbjct: 298  VLDETYRCWLQGEEEIFLDTATCAMAFRILRVNGFDISSDPFTQLSED-HFSSSLGGYMK 356

Query: 1574 GIRTLLELYKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIXXXXXXXXXXXXXXXXX 1395
             I ++LEL++ SQI+I P+E VLE  N WTS  L Q LSN  I                 
Sbjct: 357  DIGSVLELFRASQIIIHPDEFVLEKQNFWTSQFLIQELSNGSIHADGLNKYV-------- 408

Query: 1394 XXXXXXGNCQKEVVNALKFTYDADIERLENRRNIEYYNVDSLRVLKASYRCSNVGNRDIV 1215
                      +EV +ALKF Y A +ERL NRR IE YN ++ RVLK +Y  SN+GN D +
Sbjct: 409  ---------SQEVDDALKFPYHASLERLSNRRAIENYNKNNTRVLKTAYSSSNIGNEDFL 459

Query: 1214 QFGVVEDYNFCQGILQKELTSLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDA 1035
               V ED+N CQ I ++EL  L RW+ ENRLDKLKFAR KL Y YFS A  +F+PELSDA
Sbjct: 460  NLAV-EDFNICQSIQREELKDLARWITENRLDKLKFARQKLAYCYFSAAATLFSPELSDA 518

Query: 1034 RMSWKKNSFLSTVVDDFFDVKGSREELVKPYQLGREMGMQHSYQLLL*RS*NYF*SSSQD 855
            R+SW KN  L+TVVDDFFDV GS EELV   QL  +  +  S                 +
Sbjct: 519  RISWAKNGVLTTVVDDFFDVGGSVEELVNLIQLVEKWDVDVST------------DCCSE 566

Query: 854  N**PWRHRIHMTRP*CQKPSN*FHILK*FPQFQDGESTFDFRNKSDINVQISQLITVLYI 675
            N       +H T                   F D   T   RN       IS +I +   
Sbjct: 567  NVEIIFSALHST----------------ICDFADKGLTLQGRN------VISHIIDI--- 601

Query: 674  HSQWLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGSVVLITQYFLEPTLSKKVIT 495
               WL LLKSM+KE EW RDKS P M+EYM N  +SFALG +VL   Y + P LS++++ 
Sbjct: 602  ---WLNLLKSMLKEAEWLRDKSVPSMDEYMTNGYVSFALGPIVLPALYCVGPKLSEEIVG 658

Query: 494  SPKYNKLFKHMNICCRLLNDIQSFKREGEEGKLNSVLLCKIHSPEAIKEQEAVREVKGII 315
            +P+ + L++ M+ C RLLNDIQ+FKRE EEGKLN+V LC IH      ++E ++E+K  I
Sbjct: 659  TPELHHLYEIMSTCGRLLNDIQTFKRESEEGKLNAVSLCMIHGGGDCTKEETIKELKSFI 718

Query: 314  DRNRRELLGLVLNTKETIVPKGCKDLFWKTFRVTHLFYHEKDGFSSSLEMINAVNAVIHE 135
               RRELL LVL  K ++VP+ CKDLFWK  +V HLFY + DGF+S  EM N+VNAV+ E
Sbjct: 719  AGKRRELLKLVLQEKGSVVPRACKDLFWKMIKVLHLFYMKDDGFTSH-EMFNSVNAVLEE 777

Query: 134  PL 129
            P+
Sbjct: 778  PI 779


>gb|ADZ55289.1| ent-kaurene synthase [Triticum aestivum]
          Length = 846

 Score =  220 bits (561), Expect(3) = e-110
 Identities = 117/267 (43%), Positives = 160/267 (59%)
 Frame = -2

Query: 1754 VLDETYRYWLQRDDEIFLDIETCALAFRLLRINGYAVSSDTLAEFNEEIDFFTSAGGDLK 1575
            +LD  Y +WLQRD+EI +D+ TCA AFRLLR+NGY VS D L+   E  +F  S  G L 
Sbjct: 366  ILDMAYSFWLQRDEEIMMDVATCATAFRLLRMNGYDVSPDELSHLGEASNFHNSLQGYLN 425

Query: 1574 GIRTLLELYKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIXXXXXXXXXXXXXXXXX 1395
              +++LELYK S++ +S +E +L+ + +W+  LL + L ++ +                 
Sbjct: 426  DTKSVLELYKASKVSVSEHELILDNIGNWSGSLLSEKLCSERVQGLPIL----------- 474

Query: 1394 XXXXXXGNCQKEVVNALKFTYDADIERLENRRNIEYYNVDSLRVLKASYRCSNVGNRDIV 1215
                       EV  A+KF +   +ERL+++RNIE+++     +LK    C   G    +
Sbjct: 475  -----------EVEYAVKFPFYTTLERLDHKRNIEHFDARGSHILKTE--CLPYGINQEL 521

Query: 1214 QFGVVEDYNFCQGILQKELTSLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDA 1035
                VED+ F Q I Q EL  L+RWVKENRLD+L+FAR KL Y Y S A  +F PELSDA
Sbjct: 522  LALAVEDFTFSQSIYQDELLHLDRWVKENRLDQLQFARQKLTYCYLSAAATIFPPELSDA 581

Query: 1034 RMSWKKNSFLSTVVDDFFDVKGSREEL 954
            R+SW KN  L+TVVDDFFDV GS+EEL
Sbjct: 582  RISWAKNGVLTTVVDDFFDVGGSKEEL 608



 Score =  172 bits (436), Expect(3) = e-110
 Identities = 91/183 (49%), Positives = 119/183 (65%)
 Frame = -2

Query: 665  WLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGSVVLITQYFLEPTLSKKVITSPK 486
            WL LL+SMM E EW R +  P MEEYM N  +SFALG +VL   Y +   L    + + +
Sbjct: 665  WLHLLRSMMTEAEWQRSQYVPTMEEYMTNGVVSFALGPIVLPALYCVGEKLLGSAVKNQE 724

Query: 485  YNKLFKHMNICCRLLNDIQSFKREGEEGKLNSVLLCKIHSPEAIKEQEAVREVKGIIDRN 306
            Y++LF+ M+ C RLLND Q F+REG EGKLNSV L  +HS  ++  + A   ++  I  +
Sbjct: 725  YSELFRLMSTCGRLLNDSQGFEREGSEGKLNSVSLLVLHSGGSMSVEAAKNAIQKSIVAS 784

Query: 305  RRELLGLVLNTKETIVPKGCKDLFWKTFRVTHLFYHEKDGFSSSLEMINAVNAVIHEPLS 126
            RR+LL LVL  + T VP+ CK+LFWK  ++ HLFY   DGFSS  EM +AVNAVI+EPL 
Sbjct: 785  RRDLLRLVLK-EGTAVPRACKELFWKMCKILHLFYFRTDGFSSPKEMASAVNAVINEPLR 843

Query: 125  FPS 117
              S
Sbjct: 844  LSS 846



 Score = 57.0 bits (136), Expect(3) = e-110
 Identities = 27/55 (49%), Positives = 38/55 (69%)
 Frame = -3

Query: 949 NLINLVEKWECNTATNYCSEEVEIIFEALHKTINDLGDIAFT*QGRSVRSHLIDS 785
           NLI LVE W+     ++CSE+V I+F AL+ T+N LG IA   Q R V++HLI++
Sbjct: 610 NLIALVENWDEYHKDDFCSEQVRIVFCALYTTVNQLGSIASAVQNRDVKNHLIET 664


>gb|AEZ55689.1| kaurene synthase 2, partial [Salvia miltiorrhiza]
          Length = 762

 Score =  221 bits (563), Expect(3) = e-110
 Identities = 125/273 (45%), Positives = 166/273 (60%)
 Frame = -2

Query: 1754 VLDETYRYWLQRDDEIFLDIETCALAFRLLRINGYAVSSDTLAEFNEEIDFFTSAGGDLK 1575
            VLDETYR W+Q ++EIF+D  TCALAFR LR+NGY V+SD + +  +E  F +S  G++ 
Sbjct: 262  VLDETYRCWMQGNEEIFMDASTCALAFRTLRLNGYDVTSDPVTKILQEC-FSSSFRGNMT 320

Query: 1574 GIRTLLELYKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIXXXXXXXXXXXXXXXXX 1395
             I T LELY+ S++++ P+E  LE  N     LL+Q LS+ +I                 
Sbjct: 321  DINTTLELYRASELVLYPDERDLEKQNLRLKLLLEQELSSGLIQSCQLGRSINVLLI--- 377

Query: 1394 XXXXXXGNCQKEVVNALKFTYDADIERLENRRNIEYYNVDSLRVLKASYRCSNVGNRDIV 1215
                       +V  A+++ + A ++R+  R++IE YN D+ R+LK SY   N GN D  
Sbjct: 378  ----------SQVNQAIEYPFYAIMDRVAKRKSIEIYNFDNTRILKTSYCSPNFGNEDF- 426

Query: 1214 QFGVVEDYNFCQGILQKELTSLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDA 1035
             F  +ED+N CQ   ++EL  LERWV ENRLD+LKFAR K  Y YFS A   F PEL DA
Sbjct: 427  HFLSIEDFNRCQAAHREELGELERWVVENRLDELKFARSKSAYCYFSAAATFFAPELLDA 486

Query: 1034 RMSWKKNSFLSTVVDDFFDVKGSREELVKPYQL 936
            R+SW KN  L+TV+DDFFDV GS EEL    QL
Sbjct: 487  RLSWAKNGVLTTVIDDFFDVGGSVEELKNLIQL 519



 Score =  172 bits (437), Expect(3) = e-110
 Identities = 86/196 (43%), Positives = 129/196 (65%)
 Frame = -2

Query: 680  YIHSQWLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGSVVLITQYFLEPTLSKKV 501
            +I + WL LL SMM+E EWARD   P ++EYM NA +SFALG +VL   Y + P LS+ +
Sbjct: 565  HIIAIWLDLLNSMMRETEWARDNFVPTIDEYMSNAHVSFALGPIVLPALYLVGPKLSEDM 624

Query: 500  ITSPKYNKLFKHMNICCRLLNDIQSFKREGEEGKLNSVLLCKIHSPEAIKEQEAVREVKG 321
            +   +Y+ LFK M+ C RLLNDI  ++RE ++GKLN++ L  I+    + ++ A+ E+K 
Sbjct: 625  VNHSEYHNLFKLMSTCGRLLNDIHGYERELKDGKLNALSLYIINHGGEVSKEAAIWEMKS 684

Query: 320  IIDRNRRELLGLVLNTKETIVPKGCKDLFWKTFRVTHLFYHEKDGFSSSLEMINAVNAVI 141
             I+  RRELL LVL  K++++PK C++LFW    V HLFY + DGF+S  ++I  VN +I
Sbjct: 685  WIETQRRELLRLVLEGKKSVLPKPCRELFWHMCSVVHLFYSKGDGFTSQ-DLIQLVNTII 743

Query: 140  HEPLSFPSSSCEGMDE 93
            H+P+     +  G+ +
Sbjct: 744  HQPILLNDQTGAGLSK 759



 Score = 53.9 bits (128), Expect(3) = e-110
 Identities = 26/53 (49%), Positives = 35/53 (66%)
 Frame = -3

Query: 949 NLINLVEKWECNTATNYCSEEVEIIFEALHKTINDLGDIAFT*QGRSVRSHLI 791
           NLI LVE W+ +  T   S  V+IIF AL +TI ++GD AF  QGR + +H+I
Sbjct: 515 NLIQLVELWDVDICTECYSHNVQIIFSALRRTICEIGDKAFKLQGRCITNHII 567


>ref|XP_002525841.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
            communis] gi|223534846|gb|EEF36535.1| Ent-kaurene
            synthase B, chloroplast precursor, putative [Ricinus
            communis]
          Length = 778

 Score =  213 bits (543), Expect(3) = e-109
 Identities = 120/273 (43%), Positives = 167/273 (61%)
 Frame = -2

Query: 1754 VLDETYRYWLQRDDEIFLDIETCALAFRLLRINGYAVSSDTLAEFNEEIDFFTSAGGDLK 1575
            +LD+ YR W+Q  +EIF D   CA+AFR+LR NGYA+SSD LA F+E+   F       K
Sbjct: 303  MLDDIYRCWMQGSEEIFSDPTCCAMAFRILRTNGYAISSDALANFDEKESLFYE-----K 357

Query: 1574 GIRTLLELYKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIXXXXXXXXXXXXXXXXX 1395
              ++ LEL+K SQ  I  +EPVL+ +N+WTS  L++ L +  I                 
Sbjct: 358  DAKSTLELFKASQTTIFQDEPVLDKINAWTSTYLEKELRDGTIPDK-------------- 403

Query: 1394 XXXXXXGNCQKEVVNALKFTYDADIERLENRRNIEYYNVDSLRVLKASYRCSNVGNRDIV 1215
                   +   EV  ALK    A++ RLE+R  IE YNVD++ +LKASYR  NV NRD++
Sbjct: 404  -------SLHAEVDYALKHI-QANLVRLEHRSYIENYNVDNVSLLKASYRFCNVDNRDLL 455

Query: 1214 QFGVVEDYNFCQGILQKELTSLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDA 1035
             F   +DYN CQ + +KEL  LE W+K+  +D+L++AR  ++Y  FS+A  +F P+ SD 
Sbjct: 456  TFSF-QDYNMCQSMHRKELDYLEGWIKKCGIDQLEYARQTIKYAAFSIASSIFQPKFSDG 514

Query: 1034 RMSWKKNSFLSTVVDDFFDVKGSREELVKPYQL 936
            R+SW +NS L+T+VDDFFD  GS EELV   +L
Sbjct: 515  RISWAQNSVLTTIVDDFFDYGGSMEELVNLIEL 547



 Score =  170 bits (430), Expect(3) = e-109
 Identities = 87/182 (47%), Positives = 123/182 (67%)
 Frame = -2

Query: 665  WLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGSVVLITQYFLEPTLSKKVITSPK 486
            W+ LLK+M+KE EWAR+K  P M++++ N  ISFALG ++L + Y +EP LS++ + S +
Sbjct: 598  WIFLLKAMLKEAEWARNKIVPTMDDFIPNGYISFALGPIILTSLYLVEP-LSEEAVNSEE 656

Query: 485  YNKLFKHMNICCRLLNDIQSFKREGEEGKLNSVLLCKIHSPEAIKEQEAVREVKGIIDRN 306
            Y KL+  ++I  RL+ND  + + +G +GKLN V L  I+   AI E+E   +V   ID N
Sbjct: 657  YEKLYMVISILGRLINDRVATQSDGAQGKLNIVTLEVINGKGAITEEEVQEKVARTIDSN 716

Query: 305  RRELLGLVLNTKETIVPKGCKDLFWKTFRVTHLFYHEKDGFSSSLEMINAVNAVIHEPLS 126
            RRELL +V  T+ +IVPK CKD FW    V HLFY   DG+SS  +M++AVNAVI+EP+ 
Sbjct: 717  RRELLRMVSQTEGSIVPKACKDFFWTMSNVLHLFYMGDDGYSSPTKMMSAVNAVINEPIV 776

Query: 125  FP 120
             P
Sbjct: 777  LP 778



 Score = 61.2 bits (147), Expect(3) = e-109
 Identities = 29/55 (52%), Positives = 39/55 (70%)
 Frame = -3

Query: 949 NLINLVEKWECNTATNYCSEEVEIIFEALHKTINDLGDIAFT*QGRSVRSHLIDS 785
           NLI LV++W+ +T   Y S+EVEI+F A++ T NDL D A   QGR V+ H+IDS
Sbjct: 543 NLIELVQRWDDHTTIGYKSKEVEILFNAVYSTTNDLADKARILQGRCVKKHMIDS 597


>ref|XP_002525836.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
            communis] gi|223534841|gb|EEF36530.1| Ent-kaurene
            synthase B, chloroplast precursor, putative [Ricinus
            communis]
          Length = 816

 Score =  205 bits (521), Expect(3) = e-106
 Identities = 117/266 (43%), Positives = 162/266 (60%)
 Frame = -2

Query: 1751 LDETYRYWLQRDDEIFLDIETCALAFRLLRINGYAVSSDTLAEFNEEIDFFTSAGGDLKG 1572
            LD+ +R W++  DEIFLD    A+AFRLLR NGYA+SSD L++F E+   +       K 
Sbjct: 340  LDDLHRCWMKGSDEIFLDPTCSAMAFRLLRTNGYAISSDALSKFEEQKHLYNP-----KD 394

Query: 1571 IRTLLELYKTSQIMISPNEPVLETLNSWTSHLLKQNLSNDMIXXXXXXXXXXXXXXXXXX 1392
            I+ +LEL+K SQ+ I  NEP L+ + +WTS  LK+ L   +I                  
Sbjct: 395  IKCVLELFKASQMAIFQNEPTLDRIYAWTSTYLKEKLLKGVISDK--------------- 439

Query: 1391 XXXXXGNCQKEVVNALKFTYDADIERLENRRNIEYYNVDSLRVLKASYRCSNVGNRDIVQ 1212
                  + ++EV   LK  + A +ER+ENR+ IE YNVD++ + K SYR  NV  R ++ 
Sbjct: 440  ------SLREEVDFVLKHPH-ARLERIENRKFIESYNVDNVSLTKTSYRFCNVDERYLLT 492

Query: 1211 FGVVEDYNFCQGILQKELTSLERWVKENRLDKLKFARLKLEYLYFSVAGIVFTPELSDAR 1032
            F   +D+N CQ + QKEL  LERWV E RL  LK+AR K++Y YF++A  +F P+  DAR
Sbjct: 493  FAC-QDFNICQSMHQKELDDLERWVIERRLSDLKYARQKVKYAYFAIACRLFQPDFLDAR 551

Query: 1031 MSWKKNSFLSTVVDDFFDVKGSREEL 954
            +SW +NS + TVVDDFF+V GS E L
Sbjct: 552  ISWVQNSVILTVVDDFFEVGGSLEAL 577



 Score =  168 bits (426), Expect(3) = e-106
 Identities = 83/179 (46%), Positives = 120/179 (67%)
 Frame = -2

Query: 665  WLTLLKSMMKEYEWARDKSAPKMEEYMENACISFALGSVVLITQYFLEPTLSKKVITSPK 486
            WL  LKS++KE EW  +K+   M+EY+ N  IS   G ++  + YFLE  LS++++ S +
Sbjct: 634  WLIYLKSVLKEAEWVTNKTVTTMDEYISNGYISAGSGPIIFSSLYFLE-VLSEEIVNSEE 692

Query: 485  YNKLFKHMNICCRLLNDIQSFKREGEEGKLNSVLLCKIHSPEAIKEQEAVREVKGIIDRN 306
            Y  L+ H ++ CRLLND    K +G +GK NSV L  IH    I E+EAV+EV  +I+ +
Sbjct: 693  YKNLYMHTSMICRLLNDRVQAKSDGAQGKQNSVSLQVIHGNGIITEEEAVKEVTRMIEYH 752

Query: 305  RRELLGLVLNTKETIVPKGCKDLFWKTFRVTHLFYHEKDGFSSSLEMINAVNAVIHEPL 129
            RRELL +V+ T+ +IVPK CKD+FW   R+ HLFY   DG+SS  +MI+A +A+I+EP+
Sbjct: 753  RRELLRMVVETEGSIVPKACKDVFWLMSRILHLFYMSDDGYSSPTKMIHAADAIINEPI 811



 Score = 63.2 bits (152), Expect(3) = e-106
 Identities = 29/55 (52%), Positives = 40/55 (72%)
 Frame = -3

Query: 949 NLINLVEKWECNTATNYCSEEVEIIFEALHKTINDLGDIAFT*QGRSVRSHLIDS 785
           NLI LVE+W+ +    Y SEEV+I+F A++ TINDL D A+  QGR V+ HL+D+
Sbjct: 579 NLIELVERWDEHLTVGYKSEEVKILFHAIYDTINDLADKAYRKQGRCVKRHLVDT 633