BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00011331
         (1267 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16383.3| unnamed protein product [Vitis vinifera]              642   0.0  
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   642   0.0  
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   632   e-179
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   607   e-171
ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|2...   601   e-169

>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  642 bits (1656), Expect = 0.0
 Identities = 307/419 (73%), Positives = 356/419 (84%), Gaps = 7/419 (1%)
 Frame = +2

Query: 2    ENFWAACPWDEDLKYAEAVCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLC 181
            ENFW+ CPW++DLKYA+AVCDQ+ VPLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLC
Sbjct: 128  ENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLC 187

Query: 182  NTRIKFGAFMNAISSMEFDYVASGHYARVVHSSSEQDGDASVLELSNDTVKDQTYFLSHL 361
            NTRIKFGAFM+AISS EFDYVASGHYA VVHS ++Q    SVLELS D VKDQTYFLSHL
Sbjct: 188  NTRIKFGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHL 247

Query: 362  SQDQLKRLLFPLGRISKDEVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELE 541
            SQ QLKRL+FPLG I KDEVR LAT  DLPN++RKDSQGICFLGKIKFS+FVARHIGE E
Sbjct: 248  SQIQLKRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKE 307

Query: 542  GVLLEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKR 721
            G++LEAE+GD LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVSRNY+SLDKR
Sbjct: 308  GIILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKR 367

Query: 722  RRKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQ 901
            RR FR+GSLKW+S  PP   +QL CKVRHGP FY C+ TMEP +DG +DV +VQL EDDQ
Sbjct: 368  RRLFRVGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQ 427

Query: 902  GLAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE 1081
            GLAAGQF AFY+ ++C+GSG+ILESW+DQGFPVC +ALEIAR+EDKSKLGKPVKI V+PE
Sbjct: 428  GLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPE 487

Query: 1082 --PKGN-----IQLHKEAAMAKITPSEPVIETPRPASQGIFSSCFPMNWLKELRNKWLR 1237
              PKG+     I+L +E A ++   ++ +      +S+G   S FPMNWL+++R KWL+
Sbjct: 488  TPPKGSDQIDGIKLDRELANSQAAVTDEISR----SSEGEARSRFPMNWLQKIREKWLQ 542


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  642 bits (1656), Expect = 0.0
 Identities = 307/419 (73%), Positives = 356/419 (84%), Gaps = 7/419 (1%)
 Frame = +2

Query: 2    ENFWAACPWDEDLKYAEAVCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLC 181
            ENFW+ CPW++DLKYA+AVCDQ+ VPLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLC
Sbjct: 124  ENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLC 183

Query: 182  NTRIKFGAFMNAISSMEFDYVASGHYARVVHSSSEQDGDASVLELSNDTVKDQTYFLSHL 361
            NTRIKFGAFM+AISS EFDYVASGHYA VVHS ++Q    SVLELS D VKDQTYFLSHL
Sbjct: 184  NTRIKFGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHL 243

Query: 362  SQDQLKRLLFPLGRISKDEVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELE 541
            SQ QLKRL+FPLG I KDEVR LAT  DLPN++RKDSQGICFLGKIKFS+FVARHIGE E
Sbjct: 244  SQIQLKRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKE 303

Query: 542  GVLLEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKR 721
            G++LEAE+GD LGKHRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVSRNY+SLDKR
Sbjct: 304  GIILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKR 363

Query: 722  RRKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQ 901
            RR FR+GSLKW+S  PP   +QL CKVRHGP FY C+ TMEP +DG +DV +VQL EDDQ
Sbjct: 364  RRLFRVGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQ 423

Query: 902  GLAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE 1081
            GLAAGQF AFY+ ++C+GSG+ILESW+DQGFPVC +ALEIAR+EDKSKLGKPVKI V+PE
Sbjct: 424  GLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPE 483

Query: 1082 --PKGN-----IQLHKEAAMAKITPSEPVIETPRPASQGIFSSCFPMNWLKELRNKWLR 1237
              PKG+     I+L +E A ++   ++ +      +S+G   S FPMNWL+++R KWL+
Sbjct: 484  TPPKGSDQIDGIKLDRELANSQAAVTDEISR----SSEGEARSRFPMNWLQKIREKWLQ 538


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis sativus]
            gi|449524848|ref|XP_004169433.1| PREDICTED: tRNA-specific
            2-thiouridylase MnmA-like [Cucumis sativus]
          Length = 538

 Score =  632 bits (1631), Expect = e-179
 Identities = 306/421 (72%), Positives = 349/421 (82%), Gaps = 7/421 (1%)
 Frame = +2

Query: 2    ENFWAACPWDEDLKYAEAVCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLC 181
            ENFW+ CPWD+DLKYA+AVCDQ+ VPLEVVHLTEEYW NVVSYIIEEYRCGRTPNPDVLC
Sbjct: 122  ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181

Query: 182  NTRIKFGAFMNAISSMEFDYVASGHYARVVHSSSEQDGDASVLELSNDTVKDQTYFLSHL 361
            NTRIKFGAFM+AIS+MEFDYVASGHYA V+H S++Q    SVLELS D VKDQTYFLSHL
Sbjct: 182  NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241

Query: 362  SQDQLKRLLFPLGRISKDEVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELE 541
            SQ+QLKRLLFPLG I KDEVR+LA  F+LPNK+RKDSQGICFLGKIKFS+FVARHIGE E
Sbjct: 242  SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301

Query: 542  GVLLEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKR 721
            GV+LEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDI+NNVVFVSRNY+S DKR
Sbjct: 302  GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361

Query: 722  RRKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQ 901
            RR FR+GSLKWLSG PPD F +L CKVRHGPSFY C LTME  +DG +DV +V L EDDQ
Sbjct: 362  RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421

Query: 902  GLAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE 1081
            GLAAGQFAAFY+ ++C+GSG+ILESW+DQGFPVC +A+E A++EDKSKLGKP+KI V+PE
Sbjct: 422  GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481

Query: 1082 -------PKGNIQLHKEAAMAKITPSEPVIETPRPASQGIFSSCFPMNWLKELRNKWLRT 1240
                    K + ++H +    + T    V+E  R  S     +   M WL  L+ K L+ 
Sbjct: 482  SGMKESKAKDDTEIHDKLRNEEAT----VVEQIREVSPDEAMATPSMKWLGSLKKKLLQI 537

Query: 1241 F 1243
            F
Sbjct: 538  F 538


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
            tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis]
          Length = 497

 Score =  607 bits (1565), Expect = e-171
 Identities = 278/360 (77%), Positives = 323/360 (89%)
 Frame = +2

Query: 2    ENFWAACPWDEDLKYAEAVCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLC 181
            ENFW+ CPW+EDLKYA+AVCDQ+ +PLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLC
Sbjct: 111  ENFWSECPWEEDLKYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLC 170

Query: 182  NTRIKFGAFMNAISSMEFDYVASGHYARVVHSSSEQDGDASVLELSNDTVKDQTYFLSHL 361
            NTRIKFGAFM+AI++MEFDYVASGHYA+V+H S+++    SVLELS D VKDQTYFLSHL
Sbjct: 171  NTRIKFGAFMDAINNMEFDYVASGHYAKVIHPSADEINQPSVLELSKDMVKDQTYFLSHL 230

Query: 362  SQDQLKRLLFPLGRISKDEVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELE 541
            SQ QL+RL+FPLG ISKDEVR+LAT F+LPNK+RKDSQGICFLGKIKFS+FVARHIGE+E
Sbjct: 231  SQAQLRRLIFPLGGISKDEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEME 290

Query: 542  GVLLEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKR 721
            G++LEAE+GDFLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVSRNY+S DKR
Sbjct: 291  GIMLEAETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKR 350

Query: 722  RRKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQ 901
            RR  R+GSL+W +GSPP    +L CKVRHGP FY C++ +EP +   +DV +VQL EDDQ
Sbjct: 351  RRLLRVGSLRWFNGSPPKETSELQCKVRHGPGFYNCSVVIEPGEVDDQDVAIVQLCEDDQ 410

Query: 902  GLAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE 1081
            GLAAGQFAAFY+ + C+GSG+ILESW DQGFPVC RALE+AR+EDKS+LGKPVKI V+PE
Sbjct: 411  GLAAGQFAAFYKGRTCIGSGVILESWNDQGFPVCERALELARIEDKSELGKPVKIKVKPE 470


>ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|222845662|gb|EEE83209.1|
            predicted protein [Populus trichocarpa]
          Length = 434

 Score =  601 bits (1550), Expect = e-169
 Identities = 287/391 (73%), Positives = 331/391 (84%)
 Frame = +2

Query: 5    NFWAACPWDEDLKYAEAVCDQIGVPLEVVHLTEEYWSNVVSYIIEEYRCGRTPNPDVLCN 184
            NFW+ CPW+EDLK+A+ VCDQ+GVPLEVVHLT+EYWSNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 49   NFWSECPWEEDLKFAKGVCDQVGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCN 108

Query: 185  TRIKFGAFMNAISSMEFDYVASGHYARVVHSSSEQDGDASVLELSNDTVKDQTYFLSHLS 364
            TRIKFGAFM+AI++MEFDYVASGHYA V+H  S+Q    SVLELS D +KDQTYFLSHL+
Sbjct: 109  TRIKFGAFMDAINNMEFDYVASGHYAHVIHPVSDQINKPSVLELSKDKLKDQTYFLSHLT 168

Query: 365  QDQLKRLLFPLGRISKDEVRRLATNFDLPNKERKDSQGICFLGKIKFSDFVARHIGELEG 544
            Q QLKRL+FPLG I K+EVR LA+ FDLPNK+RKDSQGICFLGKIKFS+FV RHIGE EG
Sbjct: 169  QAQLKRLIFPLGCIPKEEVRNLASKFDLPNKDRKDSQGICFLGKIKFSEFVGRHIGESEG 228

Query: 545  VLLEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIQNNVVFVSRNYYSLDKRR 724
            V+LEAE+GDFLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD++NNVVFVSRNY+SLDKRR
Sbjct: 229  VILEAETGDFLGIHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRR 288

Query: 725  RKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQG 904
            R FR+GSLKWLSGSP D   QL CKVRHGP+FY C++ +E  +D  ++V  V L EDDQG
Sbjct: 289  RSFRVGSLKWLSGSPTDMTTQLQCKVRHGPTFYDCSVKIELGEDSPEEVVAVHLCEDDQG 348

Query: 905  LAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPEP 1084
            LAAGQF AFY+ + C+GSG+ILESW+D+GFPVC +ALE+ARLEDKSKLGKPVKI V+PE 
Sbjct: 349  LAAGQFTAFYQGRTCIGSGVILESWDDKGFPVCQKALELARLEDKSKLGKPVKIKVKPET 408

Query: 1085 KGNIQLHKEAAMAKITPSEPVIETPRPASQG 1177
                 + K+     I P + + E PR A  G
Sbjct: 409  SDQESVQKDG----IEPDKGLAE-PRVAVTG 434