BLASTX nr result
ID: Coptis25_contig00011181
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00011181
(1875 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002269633.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 629 e-177
ref|XP_002529475.1| hydroxysteroid dehydrogenase, putative [Rici... 588 e-165
ref|XP_004147660.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 580 e-163
ref|XP_003539040.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 579 e-163
ref|XP_004160650.1| PREDICTED: LOW QUALITY PROTEIN: 3beta-hydrox... 577 e-162
>ref|XP_002269633.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
3-like [Vitis vinifera] gi|297743842|emb|CBI36725.3|
unnamed protein product [Vitis vinifera]
Length = 581
Score = 629 bits (1621), Expect = e-177
Identities = 323/568 (56%), Positives = 406/568 (71%), Gaps = 1/568 (0%)
Frame = +1
Query: 34 CVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEKTSILSESISSGIASYFQL 213
CVV GGR FIG+ LV LLK KWIVR+ADS L LD E S+LSE+ISSG AS +
Sbjct: 10 CVVFGGRGFIGRFLVLRLLKLGKWIVRVADSAQSLQLDPSEDRSVLSEAISSGRASCCAV 69
Query: 214 DLLDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGTKNVIHACHECKVKRLIYT 393
D+ DK+Q+ KA++G++VV++ T + FY Y+ VQG +NVI+AC ECKVKRLIY
Sbjct: 70 DVRDKAQVRKAIEGASVVFYMDPTITYTNDFYLCYMIIVQGVRNVINACQECKVKRLIYN 129
Query: 394 SSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQAEALVLFANRSDGLLTTVLRPSNV 573
SSADVVFDG HD+ NG+ESLP P +F D L+D+KAQAE LVL AN DGL+T LRP NV
Sbjct: 130 SSADVVFDGSHDIYNGDESLPCPWKFEDMLTDIKAQAEGLVLIANNIDGLVTCALRPCNV 189
Query: 574 FGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAHAHVCAEEALRSRTAFVAG 753
FGPGD +LVPFL+ AKSG AKF++G+GEN+ DFTYV+NVAHAHVCAEEAL SR VAG
Sbjct: 190 FGPGDKQLVPFLVNKAKSGHAKFVIGSGENMSDFTYVENVAHAHVCAEEALVSRMVSVAG 249
Query: 754 KAFFITNLDPMKFWDFVSLIFKGLGYPRPRFKVPAKLFLFVAVLSNWLHEKLERRDAHSV 933
K FFITNL+P+KFW+FVSLI +GLGY RP FK+PA + L+V L W+ +KL+ R H V
Sbjct: 250 KVFFITNLEPVKFWEFVSLILEGLGYQRPLFKLPAGMVLYVLSLVEWMRDKLDFRSNHPV 309
Query: 934 LTPTIVHQLSCSMTFNCSEARNHIGYSPIVSLQEGVTFTIESFSHAAEDSVEVRNEDCNE 1113
+V S + TFNCS A+ HIGYSP+VSL+EGVT T ESFS ++DS + D +E
Sbjct: 310 SAQYVVQIASRTRTFNCSAAQKHIGYSPVVSLEEGVTLTTESFSQLSQDSSVMALRDFDE 369
Query: 1114 LSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLSGRTFVSSAAKLLLLISVV 1293
SK D LG G+VADILLWRDEKKTFT FL L L+FYWFFL GRTF+SSAAKLLLL++ +
Sbjct: 370 QSKVDKLLGGGKVADILLWRDEKKTFTCFLALFLMFYWFFLCGRTFISSAAKLLLLVTAI 429
Query: 1294 LFSHGILPASLFGFTVKKISPSHVEVTEK-VTDAIMYLASAWNHCMRTLSLLGRGGDWNI 1470
L GILP ++FGF++++IS S E+++ V D I +A+ WN+ + + LL GGDWN
Sbjct: 430 LSGQGILPTNIFGFSIQRISLSCFEISDTVVNDLITNMANLWNNWIHMIRLLAGGGDWNT 489
Query: 1471 FLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYEDKVDRVTEFSSTGIKSLKM 1650
F KV LY LKL+ S + V GVALV AFT+FF+Y QYE+++D + E IK K
Sbjct: 490 FFKVAIPLYFLKLILSQCLTLVVGVALVLAFTSFFVYEQYEEEMDGLAEVLFDSIKKSKR 549
Query: 1651 FLLRNVPVSVASLLDKSEIMGPKE*FPM 1734
L+RN+P + S L + + E F M
Sbjct: 550 LLMRNLPAPLVSFLCNRDALPDDEGFTM 577
>ref|XP_002529475.1| hydroxysteroid dehydrogenase, putative [Ricinus communis]
gi|223531033|gb|EEF32885.1| hydroxysteroid dehydrogenase,
putative [Ricinus communis]
Length = 579
Score = 588 bits (1516), Expect = e-165
Identities = 303/565 (53%), Positives = 393/565 (69%), Gaps = 2/565 (0%)
Frame = +1
Query: 34 CVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEKT-SILSESISSGIASYFQ 210
C V+GGR FIG+ LV LLK WIVR+ DS L LDL + S L+++ISSG ASY
Sbjct: 15 CTVVGGRGFIGRSLVFNLLKLGNWIVRVVDSAQSLQLDLTDSLDSSLNDAISSGRASYHY 74
Query: 211 LDLLDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGTKNVIHACHECKVKRLIY 390
+D++D S I KA++GS+VV++ AT + FY Y VQG KNVI AC +CKV++LIY
Sbjct: 75 IDVIDISVIAKAIEGSSVVFYMEATDLWTHDFYYCYRNVVQGAKNVIDACRQCKVRKLIY 134
Query: 391 TSSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQAEALVLFANRSDGLLTTVLRPSN 570
SSADV+FDG HD+ NG+ESLP RF D L+DLKA AEALVLFAN DGLLT LRPSN
Sbjct: 135 NSSADVIFDGSHDICNGDESLPCHWRFEDMLNDLKAHAEALVLFANNIDGLLTCALRPSN 194
Query: 571 VFGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAHAHVCAEEALRSRTAFVA 750
VFGPGD +L+PFL+ AKSG AKFI+G+GEN+ DFTYV+NVAHAH+CA EAL SR VA
Sbjct: 195 VFGPGDTQLLPFLISLAKSGCAKFIIGSGENMSDFTYVENVAHAHICAAEALDSRMVCVA 254
Query: 751 GKAFFITNLDPMKFWDFVSLIFKGLGYPRPRFKVPAKLFLFVAVLSNWLHEKLERRDAHS 930
GKAFFITNL+PMKFW+F SLI + L Y RP K+PA++ +V + W+HEKL +
Sbjct: 255 GKAFFITNLEPMKFWEFASLILEALSYQRPLIKLPAQMVWYVLLFIKWMHEKLGFSKYNH 314
Query: 931 VLTPTIVHQLSCSMTFNCSEARNHIGYSPIVSLQEGVTFTIESFSHAAEDSVEVRNEDCN 1110
++ S + TFNC A+ +IGYSP+VSL +G+ TIE++S+ +D +R +
Sbjct: 315 SMSAYFFRLASHTRTFNCIAAQKYIGYSPVVSLDDGIALTIEAYSNLVKDPSFMRCPNFE 374
Query: 1111 ELSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLSGRTFVSSAAKLLLLISV 1290
E SK D LG G+VADILLWRDEKKTFTYFL +LFYWF LSGRTF SSAA+LLLL
Sbjct: 375 EESKVDKLLGGGKVADILLWRDEKKTFTYFLIFAVLFYWFLLSGRTFTSSAARLLLLSVT 434
Query: 1291 VLFSHGILPASLFGFTVKKISPSHVEVTE-KVTDAIMYLASAWNHCMRTLSLLGRGGDWN 1467
+L+ +G LP + GFT+++IS S E++E V D++ +AS WN + + LL +G D N
Sbjct: 435 ILYGYGFLPLKISGFTIQRISLSWFEISETMVKDSVTSIASLWNRGVHNIRLLVQGKDSN 494
Query: 1468 IFLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYEDKVDRVTEFSSTGIKSLK 1647
FLKV+ LY +KL+ SF+ V G+ LVFAFTAFF+Y QYE +VD + + +GI+ K
Sbjct: 495 RFLKVLFVLYSIKLILLQSFTVVAGITLVFAFTAFFVYEQYEAEVDGLMKLLLSGIEESK 554
Query: 1648 MFLLRNVPVSVASLLDKSEIMGPKE 1722
L+R +P VAS L ++ ++
Sbjct: 555 RLLMRKLPAPVASFLQNKGMLNKED 579
>ref|XP_004147660.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
3-like [Cucumis sativus]
Length = 582
Score = 580 bits (1495), Expect = e-163
Identities = 296/554 (53%), Positives = 379/554 (68%), Gaps = 1/554 (0%)
Frame = +1
Query: 34 CVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEKTSILSESISSGIASYFQL 213
CVVLGGR F+G+ LV LLK KW VRIADS L LD E S+LS+++ +G ASYF +
Sbjct: 15 CVVLGGRGFLGRSLVLRLLKLAKWNVRIADSANSLELDPSEHDSLLSDALIAGRASYFHV 74
Query: 214 DLLDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGTKNVIHACHECKVKRLIYT 393
D+ +K QI+ A+ GS+VV++ S Y Y+ VQG KNVI AC +CKV+RLI+
Sbjct: 75 DVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVISACRDCKVRRLIFN 134
Query: 394 SSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQAEALVLFANRSDGLLTTVLRPSNV 573
SSADV+FDG D+LNG ESL YP +F D LSDLKAQAEAL+LFAN DGLLT LRPSNV
Sbjct: 135 SSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNV 194
Query: 574 FGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAHAHVCAEEALRSRTAFVAG 753
FGP D +LVPF++ AKSG AKFI+G GEN+ DFT+V+NV HAH+CAEEAL R VAG
Sbjct: 195 FGPEDTRLVPFIVHQAKSGFAKFIIGNGENMSDFTFVENVTHAHICAEEALDFRMVSVAG 254
Query: 754 KAFFITNLDPMKFWDFVSLIFKGLGYPRPRFKVPAKLFLFVAVLSNWLHEKLERRDAHSV 933
KAFFITN PMKFW+F+SLI +GLGY RP K+P+K+ V + W+ EK +
Sbjct: 255 KAFFITNFKPMKFWEFISLILRGLGYRRPSVKLPSKVVWNVLLFMKWIDEKFGFNKYNHS 314
Query: 934 LTPTIVHQLSCSMTFNCSEARNHIGYSPIVSLQEGVTFTIESFSHAAEDSVEVRNEDCNE 1113
+ SC+ TF+C+ A +GYSP+VSL+EG+T TI+SFSH ++ + E
Sbjct: 315 TWAHYIQLASCTRTFSCAAAHKQLGYSPVVSLEEGITLTIKSFSHLRKELSLASFTEFTE 374
Query: 1114 LSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLSGRTFVSSAAKLLLLISVV 1293
SKAD LG GRVAD+LLWRDEKK+FT FL LLFYWFF+ G TF+SSAA+LLL I +V
Sbjct: 375 ESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFFVRGGTFISSAAQLLLSIIIV 434
Query: 1294 LFSHGILPASLFGFTVKKISPSHVEVTEKVT-DAIMYLASAWNHCMRTLSLLGRGGDWNI 1470
L+ +G +P +++GF V+K+S + ++ V D+IM LA WN + L RG DWN
Sbjct: 435 LYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHNARALARGEDWNY 494
Query: 1471 FLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYEDKVDRVTEFSSTGIKSLKM 1650
FLK + LY+LKLL + S + + GV LVFAFTAFF+Y QYE ++D +F GI LK
Sbjct: 495 FLKAVAFLYLLKLLLARSLTMLLGVGLVFAFTAFFVYEQYEAEIDEFAKFFFIGIMELKK 554
Query: 1651 FLLRNVPVSVASLL 1692
L ++P + S L
Sbjct: 555 LLASHLPTPLMSFL 568
>ref|XP_003539040.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
3-like [Glycine max]
Length = 575
Score = 579 bits (1493), Expect = e-163
Identities = 296/558 (53%), Positives = 385/558 (68%), Gaps = 1/558 (0%)
Frame = +1
Query: 34 CVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEKTSILSESISSGIASYFQL 213
CVVLGGR F+G+ LV LLK WIVR+ADS L L E S+L +++SS ASYF +
Sbjct: 16 CVVLGGRGFLGRSLVLRLLKLGNWIVRVADSAQSLQLHHSE--SLLEQALSSSRASYFHV 73
Query: 214 DLLDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGTKNVIHACHECKVKRLIYT 393
DLLDK I+K ++GS+VV++ G FY Y VQG KNVI AC EC+V+RLIY
Sbjct: 74 DLLDKRSIVKVLEGSSVVFYMDVAGVDVNNFYTCYKLMVQGAKNVISACRECRVRRLIYN 133
Query: 394 SSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQAEALVLFANRSDGLLTTVLRPSNV 573
SSADVV G+HD+ +G+ESL YP + +TLSDLKAQAEAL+L AN DGLLT LRPSNV
Sbjct: 134 SSADVVVGGLHDIRDGDESLAYPWKTNNTLSDLKAQAEALILSANDIDGLLTCSLRPSNV 193
Query: 574 FGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAHAHVCAEEALRSRTAFVAG 753
FGPGD + VP+ L+ A+ G +KFI+GTG+N+ DFT+ +NV HAH+CAEEAL +T VAG
Sbjct: 194 FGPGDTEFVPYFLKLARYGFSKFIIGTGDNLSDFTFSENVTHAHICAEEALNFQTVSVAG 253
Query: 754 KAFFITNLDPMKFWDFVSLIFKGLGYPRPRFKVPAKLFLFVAVLSNWLHEKLERRDAHSV 933
K FFITNL+PMKFW+F+SL+ +GL Y RP K+PAKL ++ + W+HEKL R
Sbjct: 254 KTFFITNLEPMKFWEFLSLLLEGLEYQRPFIKLPAKLVQYILSVLKWVHEKLGSRYFSYP 313
Query: 934 LTPTIVHQLSCSMTFNCSEARNHIGYSPIVSLQEGVTFTIESFSHAAEDSVEVRNEDCNE 1113
L S + TFNC A+ IGYSPIVSL+EGVT TIESFSH A DS R E
Sbjct: 314 LLVHFFQLASYTRTFNCMAAQKDIGYSPIVSLEEGVTLTIESFSHLARDSSFSRCCSSTE 373
Query: 1114 LSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLSGRTFVSSAAKLLLLISVV 1293
SKAD LG G+VADILLWR+EKK+F FL LVLLFYWFFLSGRTF+S+AA+LLL +++
Sbjct: 374 QSKADKLLGGGKVADILLWREEKKSFACFLVLVLLFYWFFLSGRTFISAAARLLLFSTLL 433
Query: 1294 LFSHGILPASLFGFTVKKISPSHVEVTE-KVTDAIMYLASAWNHCMRTLSLLGRGGDWNI 1470
L+ HG LP+ LFGF +++I ++ E+++ V D++ WN + +S L +G DW+
Sbjct: 434 LYGHGFLPSKLFGFHIQRIPTTYFEISDTAVKDSVTTTVYLWNRGFQNISGLAQGDDWSA 493
Query: 1471 FLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYEDKVDRVTEFSSTGIKSLKM 1650
F K+ LY+LKL+ S F+ + G+ L AF AFF+Y QYE ++D + +F T +K +
Sbjct: 494 FFKIAVFLYLLKLILSELFTKMIGIGLAVAFMAFFVYEQYESEIDGLVDFLFTSLKEFMI 553
Query: 1651 FLLRNVPVSVASLLDKSE 1704
L+R PVS++ LL E
Sbjct: 554 HLMRISPVSISRLLHYQE 571
>ref|XP_004160650.1| PREDICTED: LOW QUALITY PROTEIN:
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
3-like [Cucumis sativus]
Length = 582
Score = 577 bits (1487), Expect = e-162
Identities = 295/554 (53%), Positives = 377/554 (68%), Gaps = 1/554 (0%)
Frame = +1
Query: 34 CVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEKTSILSESISSGIASYFQL 213
CVVLGGR F+G+ LV LLK KW VRIADS L LD E S+LS+++ +G ASYF +
Sbjct: 15 CVVLGGRGFLGRSLVLRLLKLAKWNVRIADSANSLELDPSEHDSLLSDALIAGRASYFHV 74
Query: 214 DLLDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGTKNVIHACHECKVKRLIYT 393
D+ +K QI+ A+ GS+VV++ S Y Y+ VQG KNVI AC +CKV+RLI+
Sbjct: 75 DVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVISACRDCKVRRLIFN 134
Query: 394 SSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQAEALVLFANRSDGLLTTVLRPSNV 573
SSADV+FDG D+LNG ESL YP +F D LSDLKAQAEAL+LFAN DGLLT LRPSNV
Sbjct: 135 SSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNV 194
Query: 574 FGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAHAHVCAEEALRSRTAFVAG 753
FGP D +LVPF++ AKSG AKFI+G GEN+ DFT+V+NV HAH+CAEEAL R VAG
Sbjct: 195 FGPEDTRLVPFIVHQAKSGFAKFIIGNGENMSDFTFVENVTHAHICAEEALDFRMVSVAG 254
Query: 754 KAFFITNLDPMKFWDFVSLIFKGLGYPRPRFKVPAKLFLFVAVLSNWLHEKLERRDAHSV 933
KAFFITN PMKFW+F+SLI +GLGY RP K+P+K+ V + W+ EK +
Sbjct: 255 KAFFITNFKPMKFWEFISLILRGLGYRRPSVKLPSKVVWNVLLFMKWIDEKFGFNKYNHS 314
Query: 934 LTPTIVHQLSCSMTFNCSEARNHIGYSPIVSLQEGVTFTIESFSHAAEDSVEVRNEDCNE 1113
+ SC+ TF+C+ A +GYSP+VSL+EG+T TI+SFSH ++ + E
Sbjct: 315 TWAHYIQLASCTRTFSCAAAHKQLGYSPVVSLEEGITLTIKSFSHLRKELSLASFTEFTE 374
Query: 1114 LSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLSGRTFVSSAAKLLLLISVV 1293
SKAD LG GRVAD+LLWRDEKK+FT FL LLFYWF G TF+SSAA+LLL I +V
Sbjct: 375 ESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFLXRGGTFISSAAQLLLSIIIV 434
Query: 1294 LFSHGILPASLFGFTVKKISPSHVEVTEKVT-DAIMYLASAWNHCMRTLSLLGRGGDWNI 1470
L+ +G +P +++GF V+K+S + ++ V D+IM LA WN + L RG DWN
Sbjct: 435 LYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHNARALARGEDWNY 494
Query: 1471 FLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYEDKVDRVTEFSSTGIKSLKM 1650
FLK + LY+LKLL + S + + GV LVFAFTAFF+Y QYE ++D +F GI LK
Sbjct: 495 FLKAVAFLYLLKLLLARSLTMLLGVGLVFAFTAFFVYEQYEAEIDEFAKFFFIGIMELKK 554
Query: 1651 FLLRNVPVSVASLL 1692
L ++P + S L
Sbjct: 555 LLASHLPTPLMSFL 568