BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00009966
         (1277 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002276511.1| PREDICTED: uncharacterized RNA methyltransfe...   501   e-144
emb|CAN65004.1| hypothetical protein VITISV_023490 [Vitis vinifera]   494   e-142
ref|XP_002515896.1| RNA m5u methyltransferase, putative [Ricinus...   478   e-137
ref|XP_004141670.1| PREDICTED: uncharacterized RNA methyltransfe...   477   e-132
ref|XP_003597643.1| 23S rRNA (uracil-5-)-methyltransferase rumA ...   459   e-132

>ref|XP_002276511.1| PREDICTED: uncharacterized RNA methyltransferase CT0009 [Vitis
            vinifera] gi|297743126|emb|CBI35993.3| unnamed protein
            product [Vitis vinifera]
          Length = 570

 Score =  501 bits (1290), Expect(2) = e-144
 Identities = 244/345 (70%), Positives = 283/345 (82%), Gaps = 3/345 (0%)
 Frame = +3

Query: 183  SLQVTPEEESQPSITQSPSFYPKKNQTLELVCESLAFKGKGVCKVADTGFVLMCDRALPG 362
            ++Q + E +      ++ S++PK+ QTLELVCESLAFKGKG+CKVADTGFV++CDRALPG
Sbjct: 62   AIQDSEESQFDSPKPKTQSYFPKRGQTLELVCESLAFKGKGLCKVADTGFVVLCDRALPG 121

Query: 363  ERFIGRVTRKKGNYAEVQKMKTITPHWDFVNAPCEYASYCGGCKTQNLLYEAQVRAKEQQ 542
            ERF+GRVTRKKGNYAEV K++TI+PHWD V+APCEYAS CGGCKTQNL YEAQVRAKEQQ
Sbjct: 122  ERFVGRVTRKKGNYAEVTKVETISPHWDIVDAPCEYASNCGGCKTQNLSYEAQVRAKEQQ 181

Query: 543  VSELITHVGMF--KDLELSATMKPIVPCDIQFHYRNKMEFSFATQNWLPKE-EHEKQDGL 713
            V EL+ HVG F  KD E S+ MKPIVPCD+QFHYRNKMEFSFATQ WLP+E  HEK+DG 
Sbjct: 182  VQELVIHVGKFSDKDPEFSSIMKPIVPCDVQFHYRNKMEFSFATQKWLPRELLHEKEDGA 241

Query: 714  GSYALGLHVPGFFDKVLTIDKCLLQSEAANKVLAVIQDGWRDSELGLSPYDVHTHAGFLK 893
              YALGLH PGFFDKVL ++KCLLQSE AN VLA IQ+ WRD + GLSPY+VH+H GFLK
Sbjct: 242  SGYALGLHAPGFFDKVLNVNKCLLQSEPANTVLAAIQECWRDPQFGLSPYNVHSHDGFLK 301

Query: 894  HLMLRSGSDVKTGLTELMVNFVTSAYKPELLEPLVEKISAIXXXXXXXXXXXXXXXXXXL 1073
            HL+LR+G D+KTGL ELMVNFVTS+YKPELL+PLVEKISAI                  +
Sbjct: 302  HLVLRTGRDIKTGLPELMVNFVTSSYKPELLKPLVEKISAIPEVVSIVNNINTSVGSTSV 361

Query: 1074 GEEEYILYGKPTITETLRGIIFQISANSFFQTNTHQAEVLYKLLK 1208
            GE+EYIL+GK  ITE LRG+ FQISANSFFQTNTHQAEVLYKL++
Sbjct: 362  GEQEYILHGKSAITEILRGLTFQISANSFFQTNTHQAEVLYKLIE 406



 Score = 40.0 bits (92), Expect(2) = e-144
 Identities = 17/21 (80%), Positives = 20/21 (95%)
 Frame = +2

Query: 1199 IVEENAGLRGDGSEIVLDLFC 1261
            ++E+ AGLRGDGSEIVLDLFC
Sbjct: 404  LIEDCAGLRGDGSEIVLDLFC 424


>emb|CAN65004.1| hypothetical protein VITISV_023490 [Vitis vinifera]
          Length = 1255

 Score =  494 bits (1271), Expect(2) = e-142
 Identities = 245/354 (69%), Positives = 283/354 (79%), Gaps = 12/354 (3%)
 Frame = +3

Query: 183  SLQVTPEEESQPSITQSPSFYPKKNQTLELVCESLAFKGKGVCKVADTGFVLMCDRALPG 362
            ++Q + E +      ++ S++PK+ QTLELVCESLAFKGKG+CKVADTGFV++CDRALPG
Sbjct: 34   AIQDSEESQFDSPKPKTQSYFPKRGQTLELVCESLAFKGKGLCKVADTGFVVLCDRALPG 93

Query: 363  ERFIGRVTRKKGNYAEVQKMKTITPHWDFVNAPCEYASYCGGCKTQNLLYEAQVRAKEQQ 542
            ERF+GRVTRKKGNYAEV K++TI+PHWD V+APCEYAS CGGCKTQNL YEAQVRAKEQQ
Sbjct: 94   ERFVGRVTRKKGNYAEVTKVETISPHWDIVDAPCEYASNCGGCKTQNLSYEAQVRAKEQQ 153

Query: 543  VSELITHVGMF--KDLELSATMKPIVPCDIQFHYRNKMEFSFATQNWLPKE-EHEKQDGL 713
            V EL+ HVG F  KD E S+ MKPIVPCDIQFHYRNKMEFSFATQ WLP+E  HEK+DG 
Sbjct: 154  VQELVIHVGKFSDKDPEFSSIMKPIVPCDIQFHYRNKMEFSFATQKWLPRELLHEKEDGA 213

Query: 714  GSYALGLHVPGFFDKVLTIDKCLLQSEAANKVLAVIQDGWRDSELGLSPYDVHTHAGFLK 893
              YALGLH PGFFDKVL ++KCLLQSE AN VLA IQ+ WRD + GLSPY+VH+H GFLK
Sbjct: 214  SGYALGLHAPGFFDKVLNVNKCLLQSEPANMVLAAIQECWRDPQFGLSPYNVHSHDGFLK 273

Query: 894  HLMLRSGSDVKTGLTELMVNFVTSAYKPELLEPLVEKISAIXXXXXXXXXXXXXXXXXXL 1073
            HL+LR+G D+KTGL ELMVNFVTS+YKPELL+PLVEKISAI                  +
Sbjct: 274  HLVLRTGRDIKTGLPELMVNFVTSSYKPELLKPLVEKISAIPEVVSIVNNINTSVGSTSV 333

Query: 1074 GEEEYILYGKPTITETLRGIIFQISANSFFQTNTH---------QAEVLYKLLK 1208
            GE+EYIL+GK  ITE LRG+ FQISANSFFQTNTH         QAEVLYKL++
Sbjct: 334  GEQEYILHGKSAITEILRGLTFQISANSFFQTNTHQQILRLYNVQAEVLYKLIE 387



 Score = 40.0 bits (92), Expect(2) = e-142
 Identities = 17/21 (80%), Positives = 20/21 (95%)
 Frame = +2

Query: 1199 IVEENAGLRGDGSEIVLDLFC 1261
            ++E+ AGLRGDGSEIVLDLFC
Sbjct: 385  LIEDCAGLRGDGSEIVLDLFC 405


>ref|XP_002515896.1| RNA m5u methyltransferase, putative [Ricinus communis]
            gi|223544801|gb|EEF46316.1| RNA m5u methyltransferase,
            putative [Ricinus communis]
          Length = 577

 Score =  478 bits (1229), Expect(2) = e-137
 Identities = 233/331 (70%), Positives = 271/331 (81%), Gaps = 3/331 (0%)
 Frame = +3

Query: 225  TQSPSFYPKKNQTLELVCESLAFKGKGVCKVADTGFVLMCDRALPGERFIGRVTRKKGNY 404
            ++S + +PK+ Q+LELVCESLAFKGKGVCKVADTGFV+MCDRALPGERFIGRVTR+KG+Y
Sbjct: 83   SKSGALFPKRGQSLELVCESLAFKGKGVCKVADTGFVVMCDRALPGERFIGRVTRRKGSY 142

Query: 405  AEVQKMKTITPHWDFVNAPCEYASYCGGCKTQNLLYEAQVRAKEQQVSELITHVGMFKDL 584
            AEV K+KTI+PHWD + APCEYASYCGGCKTQNL YEAQVRAKE QV EL+ ++G F D+
Sbjct: 143  AEVTKVKTISPHWDIIEAPCEYASYCGGCKTQNLSYEAQVRAKELQVRELVINIGKFSDI 202

Query: 585  --ELSATMKPIVPCDIQFHYRNKMEFSFATQNWLPKE-EHEKQDGLGSYALGLHVPGFFD 755
              E  + MKPIVPCD++FHYRNKMEFSF ++ WLP+E   EKQDG  +YALGLH PG FD
Sbjct: 203  DPEFVSIMKPIVPCDVKFHYRNKMEFSFGSKKWLPRELLGEKQDGSVNYALGLHAPGCFD 262

Query: 756  KVLTIDKCLLQSEAANKVLAVIQDGWRDSELGLSPYDVHTHAGFLKHLMLRSGSDVKTGL 935
            KVL +DKCLLQSE AN VLA +QD WRDS+ GL+PYDVH+H GFLKHLMLR+G + +TGL
Sbjct: 263  KVLNVDKCLLQSEPANTVLATVQDCWRDSQFGLTPYDVHSHDGFLKHLMLRTGRNTETGL 322

Query: 936  TELMVNFVTSAYKPELLEPLVEKISAIXXXXXXXXXXXXXXXXXXLGEEEYILYGKPTIT 1115
             ELMVNFVTSAYKPELL+ +VEKISAI                  +GEEEY +YGK TIT
Sbjct: 323  LELMVNFVTSAYKPELLKIIVEKISAIPEVVSIVNNVNSSVGNTSVGEEEYTIYGKSTIT 382

Query: 1116 ETLRGIIFQISANSFFQTNTHQAEVLYKLLK 1208
            E LRG+ FQISANSFFQTNT QAEVLYKL++
Sbjct: 383  EILRGLTFQISANSFFQTNTRQAEVLYKLIE 413



 Score = 40.0 bits (92), Expect(2) = e-137
 Identities = 17/21 (80%), Positives = 20/21 (95%)
 Frame = +2

Query: 1199 IVEENAGLRGDGSEIVLDLFC 1261
            ++E+ AGLRGDGSEIVLDLFC
Sbjct: 411  LIEDCAGLRGDGSEIVLDLFC 431


>ref|XP_004141670.1| PREDICTED: uncharacterized RNA methyltransferase BT_0643-like
            [Cucumis sativus] gi|449480609|ref|XP_004155944.1|
            PREDICTED: uncharacterized RNA methyltransferase
            BT_0643-like [Cucumis sativus]
          Length = 562

 Score =  477 bits (1228), Expect = e-132
 Identities = 236/348 (67%), Positives = 278/348 (79%), Gaps = 7/348 (2%)
 Frame = +3

Query: 225  TQSPSFYPKKNQTLELVCESLAFKGKGVCKVADTGFVLMCDRALPGERFIGRVTRKKGNY 404
            ++S S++PK+ QTLELVCESLAFKGKG+CKV+DTGFV+MCDR LPGERF+GRVTR+KGNY
Sbjct: 61   SKSQSYFPKRGQTLELVCESLAFKGKGLCKVSDTGFVVMCDRVLPGERFVGRVTRRKGNY 120

Query: 405  AEVQKMKTITPHWDFVNAPCEYASYCGGCKTQNLLYEAQVRAKEQQVSELITHVGMF--K 578
            AEV K++T++PHWD V+APCEYASYCGGCKTQNL YEAQ+R KEQQV EL+THVG F  K
Sbjct: 121  AEVTKLETLSPHWDLVDAPCEYASYCGGCKTQNLSYEAQLRYKEQQVHELLTHVGKFSQK 180

Query: 579  DLELSATMKPIVPCDIQFHYRNKMEFSFATQNWLPKEE-HEKQDGLGSYALGLHVPGFFD 755
            +LE    MKPIVPC+IQFHYRNKMEFSF ++ W+PK+   EKQ+G    ALGLH PGFFD
Sbjct: 181  ELEFHIVMKPIVPCEIQFHYRNKMEFSFGSKRWVPKDALKEKQEGDEINALGLHAPGFFD 240

Query: 756  KVLTIDKCLLQSEAANKVLAVIQDGWRDSELGLSPYDVHTHAGFLKHLMLRSGSDVKTGL 935
            KVL +DKCLLQS  AN+VLA +Q+ WRD  LGLSPYDVH+H GFLKHLMLR+G DV TGL
Sbjct: 241  KVLNVDKCLLQSGPANQVLASVQECWRDPLLGLSPYDVHSHKGFLKHLMLRTGRDVNTGL 300

Query: 936  TELMVNFVTSAYKPELLEPLVEKISAIXXXXXXXXXXXXXXXXXXLGEEEYILYGKPTIT 1115
             ELMVNFVTS+YKPELL PLVEK S I                  +GEEE++LYGK +I 
Sbjct: 301  PELMVNFVTSSYKPELLMPLVEKFSDIPEVVSVVNNINSSVGNTSVGEEEHVLYGKSSIR 360

Query: 1116 ETLRGIIFQISANSFFQTNTHQAEVLYKLLKRMLVLE----EMVQKLF 1247
            E+LRG+ FQISANSFFQTNTHQAEVLY+L++    L+    E+V  LF
Sbjct: 361  ESLRGLTFQISANSFFQTNTHQAEVLYRLIEECAGLKGDGSEIVLDLF 408


>ref|XP_003597643.1| 23S rRNA (uracil-5-)-methyltransferase rumA [Medicago truncatula]
            gi|355486691|gb|AES67894.1| 23S rRNA
            (uracil-5-)-methyltransferase rumA [Medicago truncatula]
          Length = 560

 Score =  459 bits (1180), Expect(2) = e-132
 Identities = 227/348 (65%), Positives = 271/348 (77%), Gaps = 11/348 (3%)
 Frame = +3

Query: 198  PEEESQPSITQSP------SFYPKKNQTLELVCESLAFKGKGVCKVADTGFVLMCDRALP 359
            PE  +  SI ++P      S++PK+ QTLEL+CESLAFKGKG+CKV DTGFV+MCDRALP
Sbjct: 49   PEPTTTTSIPKTPNPNTSTSYFPKRGQTLELLCESLAFKGKGLCKVTDTGFVVMCDRALP 108

Query: 360  GERFIGRVTRKKGNYAEVQKMKTITPHWDFVNAPCEYASYCGGCKTQNLLYEAQVRAKEQ 539
            GERFIGR+TRKKGNYAE  K++T+TP +  V+APC Y  +CGGCKTQNL Y AQV AKE+
Sbjct: 109  GERFIGRITRKKGNYAEATKVETLTPPFHVVDAPCVYVPHCGGCKTQNLAYLAQVNAKEE 168

Query: 540  QVSELITHVGMF--KDLELSATMKPIVPCDIQFHYRNKMEFSFATQNWLPKEE-HEKQDG 710
            QV +L+ HVG F  K+LEL   MKPIVPCDIQFHYRNKMEFSF    WLPKE  HE+   
Sbjct: 169  QVRDLVIHVGRFSQKELELHGIMKPIVPCDIQFHYRNKMEFSFGPYKWLPKESLHERNVD 228

Query: 711  LGS--YALGLHVPGFFDKVLTIDKCLLQSEAANKVLAVIQDGWRDSELGLSPYDVHTHAG 884
             GS  Y+LGLHVPGFFDK++ ++KCLLQ++ ANKVLA IQ+ WRD +LG SPY+VH+H G
Sbjct: 229  AGSENYSLGLHVPGFFDKIINVEKCLLQTDPANKVLAAIQECWRDPQLGFSPYNVHSHKG 288

Query: 885  FLKHLMLRSGSDVKTGLTELMVNFVTSAYKPELLEPLVEKISAIXXXXXXXXXXXXXXXX 1064
            FLKHLMLRSG DV TGL E+MVNFVTS+YKP+LL+ LV+K+SA                 
Sbjct: 289  FLKHLMLRSGRDVMTGLPEVMVNFVTSSYKPDLLKVLVDKVSAFPEVVSIMNNVNTSVGN 348

Query: 1065 XXLGEEEYILYGKPTITETLRGIIFQISANSFFQTNTHQAEVLYKLLK 1208
              +GEEEY L+GK +ITETLRG+ FQISANSFFQTNTHQAEVLYKL++
Sbjct: 349  TSVGEEEYTLHGKSSITETLRGLTFQISANSFFQTNTHQAEVLYKLIE 396



 Score = 40.0 bits (92), Expect(2) = e-132
 Identities = 16/21 (76%), Positives = 20/21 (95%)
 Frame = +2

Query: 1199 IVEENAGLRGDGSEIVLDLFC 1261
            ++EE AG+RGDGSE+VLDLFC
Sbjct: 394  LIEECAGIRGDGSEVVLDLFC 414