BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00009535
         (1697 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   881   0.0  
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   789   0.0  
dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       686   0.0  
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       647   0.0  
gb|ACO90219.1| stylopine synthase [Eschscholzia californica]          615   e-173

>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
            Full=(S)-tetrahydrocolumbamine oxidase
            (methylenedioxy-bridge-forming); AltName: Full=Cytochrome
            P450 719A1 gi|15822820|dbj|BAB68769.1| methylenedioxy
            bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  881 bits (2276), Expect = 0.0
 Identities = 449/491 (91%), Positives = 451/491 (91%)
 Frame = -2

Query: 1552 MEMSPLLXXXXXXXXXXXXXXIRILFSSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVA 1373
            MEM+PLL              IRILFSSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVA
Sbjct: 1    MEMNPLLVCATVAIVFATTTIIRILFSSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVA 60

Query: 1372 LAKLAKVHGSVMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGARDMPEITKIASASWHT 1193
            LAKLAKVHGSVMTIWIGSWRPVIVISD+EKAWEVLVNKSADYGARDMPEITKIASASWHT
Sbjct: 61   LAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVLVNKSADYGARDMPEITKIASASWHT 120

Query: 1192 ISTSDAGPFWQNVRKGLQSGAMGPFNVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLD 1013
            ISTSDAG FWQNVRKGLQSGAMGP NVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLD
Sbjct: 121  ISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLD 180

Query: 1012 HIKKNTVRLLTRLIFGQTFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKA 833
            HIKKNTVRLLTRLIFGQ FDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKA
Sbjct: 181  HIKKNTVRLLTRLIFGQAFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKA 240

Query: 832  EREAFLVKCRVEELVRPLLSSNPPTNSYLYFLLSQNXXXXXXXXXXXELYLLGVDSTSST 653
            EREAFLVKCRVEELVRPLLSS PPTNSYLYFLLSQN           ELYLLGVDSTSST
Sbjct: 241  EREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQNFEEEVIIFCIFELYLLGVDSTSST 300

Query: 652  TTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLA 473
            TTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLA
Sbjct: 301  TTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLA 360

Query: 472  IPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFLEGETGTAYNKAM 293
            IPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFLEGETGTAYNKAM
Sbjct: 361  IPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFLEGETGTAYNKAM 420

Query: 292  EQSFLPFSAGMRICAGMDLGKLQXXXXXXXXXXXFKWSCVEEGKLPDMGEELSFVLLMKT 113
            EQSFLPFSAGMRICAGMDLGKLQ           FKWSCVEEGKLPDMGEELSFVLLMKT
Sbjct: 421  EQSFLPFSAGMRICAGMDLGKLQFAFALANLVNAFKWSCVEEGKLPDMGEELSFVLLMKT 480

Query: 112  PLEARIAGRNV 80
            PLEARIAGRNV
Sbjct: 481  PLEARIAGRNV 491


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  789 bits (2037), Expect = 0.0
 Identities = 392/463 (84%), Positives = 418/463 (90%)
 Frame = -2

Query: 1474 SSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVIS 1295
            SSSS  QMKWP+GP KLPIIGNLHQLG DVLHVALAKLA+VHGSVMT+WIG+WRP+IVIS
Sbjct: 28   SSSSFSQMKWPTGPNKLPIIGNLHQLGGDVLHVALAKLAQVHGSVMTVWIGNWRPIIVIS 87

Query: 1294 DLEKAWEVLVNKSADYGARDMPEITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGPFN 1115
            D++KAWEVLVNKS+DYGARDMPEITKIASASWHTISTSDAG FWQ VRKGLQSGAMGP N
Sbjct: 88   DIDKAWEVLVNKSSDYGARDMPEITKIASASWHTISTSDAGAFWQTVRKGLQSGAMGPLN 147

Query: 1114 VAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQTFDDNKFIE 935
            +AAQ+Q+QERDMKRLI+A+ DEAA NN IVKPLDHIKKNTVRLLTRLIFGQTFDD+KF+E
Sbjct: 148  IAAQSQFQERDMKRLIQALRDEAAQNNNIVKPLDHIKKNTVRLLTRLIFGQTFDDDKFVE 207

Query: 934  SMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSNPPTN 755
            SMH+EI+DIIRISGYARLAEAFYYAKYLPSHKKAEREA+LVKCRVE+LV PLLSSNPPTN
Sbjct: 208  SMHFEIDDIIRISGYARLAEAFYYAKYLPSHKKAEREAYLVKCRVEDLVSPLLSSNPPTN 267

Query: 754  SYLYFLLSQNXXXXXXXXXXXELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTL 575
            SYLYFLLSQ            ELYLLGVDSTSSTTTWALAYLIREQ  QEKLYQD RMTL
Sbjct: 268  SYLYFLLSQRFSEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQAIQEKLYQDTRMTL 327

Query: 574  GDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNL 395
            GDVDLVKIEDV+KLKYLQ VVKETMRMKPIAPLAIPHKTAK+TTLMG+KVAKGTRIMVNL
Sbjct: 328  GDVDLVKIEDVSKLKYLQAVVKETMRMKPIAPLAIPHKTAKDTTLMGSKVAKGTRIMVNL 387

Query: 394  YALHHNQNIWPDPYKFMPERFLEGETGTAYNKAMEQSFLPFSAGMRICAGMDLGKLQXXX 215
            YALHHNQNIW +PYKFMPERFL+GE G+A NKAMEQSFLPFSAGMRICAGMDLGKLQ   
Sbjct: 388  YALHHNQNIWTNPYKFMPERFLQGEDGSASNKAMEQSFLPFSAGMRICAGMDLGKLQFAF 447

Query: 214  XXXXXXXXFKWSCVEEGKLPDMGEELSFVLLMKTPLEARIAGR 86
                    FKWSCVEEGK PDM EELSFVLLMKTPLEA+I  R
Sbjct: 448  ALANLVNAFKWSCVEEGKFPDMSEELSFVLLMKTPLEAKITAR 490


>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  686 bits (1769), Expect = 0.0
 Identities = 340/465 (73%), Positives = 384/465 (82%)
 Frame = -2

Query: 1474 SSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVIS 1295
            SSSS  + KWPSGP++LPIIGNLH LG D+LHV LAKLAKVHGSVMTIWIG WRP+IVIS
Sbjct: 27   SSSSSSKKKWPSGPKRLPIIGNLHLLGGDLLHVTLAKLAKVHGSVMTIWIGRWRPIIVIS 86

Query: 1294 DLEKAWEVLVNKSADYGARDMPEITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGPFN 1115
            D+  AWEVLV+KSA+YG RD P+I K  +   + IS SD GPFW NVRKGLQ+GA+ P N
Sbjct: 87   DINSAWEVLVSKSAEYGQRDTPDIFKTLTVGQNNISMSDIGPFWHNVRKGLQNGALSPLN 146

Query: 1114 VAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQTFDDNKFIE 935
            VAAQ Q++E D+KRLI  MS EA N NGI+KPLDH+KKNT RLL RLIFG TFD++KF+E
Sbjct: 147  VAAQTQFREEDVKRLIHEMSREALNKNGIIKPLDHMKKNTARLLGRLIFGPTFDNDKFVE 206

Query: 934  SMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSNPPTN 755
            S+ YEIED++R+SGYARL EAFYYAKYLPS KK  REA+L K R+EEL+RPLLSSNPPTN
Sbjct: 207  SVLYEIEDVLRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEELMRPLLSSNPPTN 266

Query: 754  SYLYFLLSQNXXXXXXXXXXXELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTL 575
            SYLYFL SQN           EL+LLGVDSTSST TWALAYLI EQ  QEKL +DIRMT+
Sbjct: 267  SYLYFLQSQNYSKEIIIFCTFELFLLGVDSTSSTATWALAYLIHEQAVQEKLNEDIRMTI 326

Query: 574  GDVDLVKIEDVNKLKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNL 395
            GDVDLVKIED++KLKYLQ VVKETMRMKPIAPLAIPHKTA +TTLMGTKVA+GT+IMVNL
Sbjct: 327  GDVDLVKIEDLSKLKYLQAVVKETMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNL 386

Query: 394  YALHHNQNIWPDPYKFMPERFLEGETGTAYNKAMEQSFLPFSAGMRICAGMDLGKLQXXX 215
            YALHHN++IWP+PYKFMPERFL+GE  +A NKAMEQSFLPF AGMRICAGMDLGKLQ   
Sbjct: 387  YALHHNEDIWPEPYKFMPERFLQGENVSANNKAMEQSFLPFGAGMRICAGMDLGKLQIAF 446

Query: 214  XXXXXXXXFKWSCVEEGKLPDMGEELSFVLLMKTPLEARIAGRNV 80
                    FKWSCV+EGKLPDM E+L  V LMKTPLE RI+ R V
Sbjct: 447  ALANLVNAFKWSCVDEGKLPDMSEQLFAVTLMKTPLEGRISTRKV 491


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  647 bits (1668), Expect = 0.0
 Identities = 333/497 (67%), Positives = 383/497 (77%), Gaps = 5/497 (1%)
 Frame = -2

Query: 1555 EMEMSPLLXXXXXXXXXXXXXXIRILFS-SSSLPQMKWPSGPRKLPIIGNLHQLGDDVLH 1379
            EME S LL              +RI  S SSS  QM WPSGP+KLPIIGNLHQL  ++LH
Sbjct: 12   EMEGSLLLAGATLAILLTISTIVRITSSPSSSFSQMTWPSGPKKLPIIGNLHQLRGELLH 71

Query: 1378 VALAKLAKVHGSVMTIWIGSWRPVIVISDLEKAWEVLVNKSADYGARDMP---EITKIAS 1208
            V LAKLAKVHG VMTIW+GSW P+IVISD+  AWEVLVNKSADYGAR++    E+ + ++
Sbjct: 72   VTLAKLAKVHGGVMTIWMGSWVPIIVISDINSAWEVLVNKSADYGAREVTYLHELLRDSA 131

Query: 1207 ASWHTISTSDAGPFWQNVRKGLQSGAMGPFNVAAQNQYQERDMKRLIKAMSDEAANNNGI 1028
               H IS+SD GP W N+RKGLQSGA+ P +VA+Q Q++ERDMKR++  MS E  NNNG 
Sbjct: 132  MHPHNISSSDVGPLWHNIRKGLQSGALSPLSVASQTQHRERDMKRVVHEMSREIVNNNGT 191

Query: 1027 VKPLDHIKKNTVRLLTRLIFGQTFDDNKFIESMHYEIEDIIRISGYARLAEAFYYAKYLP 848
            +KPL HIK +T RLL RLIFG +F+D+KFIESM YEIED IR S Y  L  +FYYAKYLP
Sbjct: 192  IKPLVHIKNSTARLLGRLIFGPSFNDSKFIESMLYEIEDFIRTSSYTSLTNSFYYAKYLP 251

Query: 847  SHKKAEREAFLVKCRVEELVRPLLSSNPPTNSYLYFLLSQNXXXXXXXXXXXELYLLGVD 668
            S +KA REA L K R+EELVRPLLSS PPTN+YLYFLLSQN           EL+LLGVD
Sbjct: 252  SQRKATREAKLGKYRIEELVRPLLSSKPPTNAYLYFLLSQNLSEEVTIFCIFELFLLGVD 311

Query: 667  STSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIEDVNKLKYLQGVVKETMRMKP 488
            S SSTTTWALAY+IREQ AQEKLYQDIRMT+GDVDLVKIEDVNKLKYLQ VVKETMRMKP
Sbjct: 312  SASSTTTWALAYMIREQEAQEKLYQDIRMTIGDVDLVKIEDVNKLKYLQSVVKETMRMKP 371

Query: 487  IAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALHHNQNIWPDPYKFMPERFLEGETGTA 308
            IAPLA+PH   K+TTLMGTK+ KGTR+MVNLYALHH +++W +P KFMPER+L+GE G+ 
Sbjct: 372  IAPLALPHNAGKDTTLMGTKIVKGTRVMVNLYALHHYEDVWLEPSKFMPERYLQGEDGSV 431

Query: 307  YNKAMEQSFLPFSAGMRICAGMDLGKLQXXXXXXXXXXXFKWSCVEEGKLPDMGEEL-SF 131
             +KAMEQS LPF AGMRICAGMDLGKLQ           FKWSCVE+GKLPDM EEL   
Sbjct: 432  THKAMEQSLLPFGAGMRICAGMDLGKLQVAFVLANLVNAFKWSCVEDGKLPDMTEELFGL 491

Query: 130  VLLMKTPLEARIAGRNV 80
            ++LMKTPLE RI+ R V
Sbjct: 492  IILMKTPLEGRISPRTV 508


>gb|ACO90219.1| stylopine synthase [Eschscholzia californica]
          Length = 495

 Score =  615 bits (1586), Expect = e-173
 Identities = 300/468 (64%), Positives = 368/468 (78%), Gaps = 2/468 (0%)
 Frame = -2

Query: 1480 LFSSSSLPQMKWPSGPRKLPIIGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIV 1301
            LF +    +M WP+GP+ LPIIGN+H LG   L V L  LAKVHGSVMTIWIGSWRPVIV
Sbjct: 28   LFFTRKSSKMVWPAGPKTLPIIGNMHLLGGTALQVVLHNLAKVHGSVMTIWIGSWRPVIV 87

Query: 1300 ISDLEKAWEVLVNKSADYGARDMPEITKIASASWHTISTSDAGPFWQNVRKGLQSGAMGP 1121
            +SD+E+AWEVLVNKS+DY ARDMP+ITKI SA W TISTSD+GP W N+RKGLQ+ A+ P
Sbjct: 88   VSDIERAWEVLVNKSSDYSARDMPDITKIISADWKTISTSDSGPHWTNLRKGLQNVALSP 147

Query: 1120 FNVAAQNQYQERDMKRLIKAMSDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQTFDDNKF 941
             N+AAQ Q+QE+DM ++I+ + +EA NNNGIVKPLDH+KK T+RL++RL+FGQ F+++K+
Sbjct: 148  HNLAAQFQFQEKDMTKMIQTLQEEARNNNGIVKPLDHMKKATLRLISRLVFGQDFNNDKY 207

Query: 940  IESMHYEIEDIIRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSNPP 761
            ++ MH  IE++IR+SGYARLAEAFYYAKYLPSHKKA RE    + RV+ LV P LS NPP
Sbjct: 208  VDDMHLAIEELIRVSGYARLAEAFYYAKYLPSHKKAVREVEEAQRRVQNLVSPFLSLNPP 267

Query: 760  TNSYLYFLLSQNXXXXXXXXXXXELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRM 581
            TN+YL+FL SQ            E YLLGVDSTS TT WALA+LIRE   QEKLYQ++  
Sbjct: 268  TNTYLHFLRSQKYDDEVIIFAIFEAYLLGVDSTSLTTAWALAFLIRESNVQEKLYQELES 327

Query: 580  TLGDVD--LVKIEDVNKLKYLQGVVKETMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRI 407
                +D  ++K+ED+NKL+YLQ V+KETMRMKPIAPLAIPHK  ++T+LMG K+ KGTR+
Sbjct: 328  FASKMDRKILKVEDINKLQYLQAVIKETMRMKPIAPLAIPHKACRDTSLMGKKIDKGTRV 387

Query: 406  MVNLYALHHNQNIWPDPYKFMPERFLEGETGTAYNKAMEQSFLPFSAGMRICAGMDLGKL 227
            MVN+YALHHN+N++ DP+KFMPERF++ ++  A  KAMEQS LPFSAGMRICAGM+LGKL
Sbjct: 388  MVNIYALHHNKNVFNDPFKFMPERFMKVDSQDANGKAMEQSLLPFSAGMRICAGMELGKL 447

Query: 226  QXXXXXXXXXXXFKWSCVEEGKLPDMGEELSFVLLMKTPLEARIAGRN 83
            Q           FKWSCV +G LPDM ++L FVLLMKTPLEARI  RN
Sbjct: 448  QFSFALANLAYAFKWSCVADGVLPDMSDQLGFVLLMKTPLEARINRRN 495