BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00009253
         (1265 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       505   e-140
gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago ...   440   e-121
dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula]   438   e-120
dbj|BAL45206.1| cytochrome P450 monooxygenase [Glycyrrhiza urale...   427   e-117
ref|XP_003546752.1| PREDICTED: secologanin synthase-like [Glycin...   427   e-117

>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  505 bits (1300), Expect = e-140
 Identities = 242/372 (65%), Positives = 303/372 (81%)
 Frame = -2

Query: 1120 AWKIFNYIWLTPKKLEKYLRDQGISGPPYKLLTGNLKELGSMMMEAKSKPMELSHRIVPR 941
            AWK F ++WL P+KLEK LR+QGI GP YK L+GNLK++ S   EA++KPMELSH+IVPR
Sbjct: 22   AWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDIVSATQEARAKPMELSHKIVPR 81

Query: 940  VNPFTLQTVQKHGKISLEWYGPTLRVNITDPDHIGEVLSNKHGHIFRAQSTPFLKLLVTG 761
            V PF  QTV+K+GK+S  WYGP  RVNI DPD I ++LSNK GH  + ++ P  KLL++G
Sbjct: 82   VMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILSNKFGHFEKIRTNPLGKLLISG 141

Query: 760  LISYDGDKWVKHRKLINPAFHLEKLKIMLPAFYTSCIELVSKWQNLISKESCELDVWPEF 581
            ++SY+G+KWVKHR+++NPAFHLEKLK+MLPA YTSC E+V+KW+ ++S++SCELDVWP  
Sbjct: 142  VVSYEGEKWVKHRRILNPAFHLEKLKMMLPAIYTSCSEMVNKWEMMVSQDSCELDVWPHL 201

Query: 580  QKLTSDVISRSAFGVSYVQGLRINQLESELAELLVQSSIQAPYIPGISWYLPTKTNNRMK 401
            Q LT+DVISR+AFG SY +G RI QL+SE A L+++ ++Q+ YIPG+  +LPTK NNRMK
Sbjct: 202  QNLTADVISRTAFGSSYEEGRRIFQLQSEQAGLVIK-ALQSIYIPGLR-FLPTKRNNRMK 259

Query: 400  EIEAEVRTLLTNLVKQREEATKIGEVRVNDLLGLLLESNMKEIEDNGTSKSKGMSIDEII 221
            EI  E R LL ++V +RE+A KIGE   +DLLGLL+ESN KEI DN  SK+ GM+IDE+I
Sbjct: 260  EIYGEARVLLRDIVNKREKAMKIGEAHNDDLLGLLMESNFKEITDNVNSKNFGMTIDEVI 319

Query: 220  DECKLFYIAGQETIATLLVWTTIVLSMHPEWQVKAREEVFEVFGKSKPDFEGLNRLKIVT 41
            DECKLFY AGQET +TLLVWT +VLSMHP+WQ KAREEV +VFG   PDF+GLN LKIVT
Sbjct: 320  DECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVT 379

Query: 40   MILYEVLRLYPP 5
            MILYEVLRLYPP
Sbjct: 380  MILYEVLRLYPP 391


>gb|ABC59078.1| cytochrome P450 monooxygenase CYP72A59 [Medicago truncatula]
          Length = 518

 Score =  440 bits (1131), Expect = e-121
 Identities = 218/375 (58%), Positives = 282/375 (75%), Gaps = 2/375 (0%)
 Frame = -2

Query: 1120 AWKIFNYIWLTPKKLEKYLRDQGISGPPYKLLTGNLKELGSMMMEAKSKPMELSHRIVPR 941
            AW+I N++WL PKKLEK LR+QG+ G  Y+LL G++ +L  M  EA SKPM LS  IVPR
Sbjct: 20   AWRILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDMNDLLKMRKEATSKPMNLSDDIVPR 79

Query: 940  VNPFTLQTVQKHGKISLEWYGPTLRVNITDPDHIGEVLSNKHGHIFRAQSTPFLKLLVTG 761
            V  F  Q+V KHGK S  W+GPT RVNITDPD I +VL NK     +  + P ++LL  G
Sbjct: 80   VYSFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVL-NKIYDFQKPNANPLVRLLANG 138

Query: 760  LISYDGDKWVKHRKLINPAFHLEKLKIMLPAFYTSCIELVSKWQNLISKE-SCELDVWPE 584
            L+S +G++W KHRK+INPAFHLEKLKIMLP F+ SC +L+SKW+ ++S + SCE+DVWP 
Sbjct: 139  LVSLEGEQWSKHRKIINPAFHLEKLKIMLPIFHKSCDDLISKWEKMLSSDGSCEMDVWPF 198

Query: 583  FQKLTSDVISRSAFGVSYVQGLRINQLESELAELLVQSSIQAPYIPGISW-YLPTKTNNR 407
             Q   SDVISR+AFG SY +G RI QL++E AEL  +  ++  YIPG  W +LPT T  R
Sbjct: 199  LQNFASDVISRAAFGSSYEEGKRIFQLQTEQAELTTKIMMKV-YIPG--WRFLPTATPRR 255

Query: 406  MKEIEAEVRTLLTNLVKQREEATKIGEVRVNDLLGLLLESNMKEIEDNGTSKSKGMSIDE 227
            MKEI+ +++  LT+++  RE A K GE   +DLLG+LLESN KE+E++G +K  GMS+D+
Sbjct: 256  MKEIDRDIKASLTDMINNRERALKAGEATKDDLLGILLESNHKEMEEHGNNKDVGMSLDD 315

Query: 226  IIDECKLFYIAGQETIATLLVWTTIVLSMHPEWQVKAREEVFEVFGKSKPDFEGLNRLKI 47
            +I+ECKLFY AGQET + LLVWT ++LS +P+WQ +AREEV +VFG +KPDF+GL+ LKI
Sbjct: 316  VIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVLQVFGNNKPDFDGLSHLKI 375

Query: 46   VTMILYEVLRLYPPV 2
            VTMILYEVLRLYPPV
Sbjct: 376  VTMILYEVLRLYPPV 390


>dbj|BAL45198.1| cytochrome P450 monooxygenase [Medicago truncatula]
          Length = 518

 Score =  438 bits (1127), Expect = e-120
 Identities = 216/375 (57%), Positives = 283/375 (75%), Gaps = 2/375 (0%)
 Frame = -2

Query: 1120 AWKIFNYIWLTPKKLEKYLRDQGISGPPYKLLTGNLKELGSMMMEAKSKPMELSHRIVPR 941
            AW+I N++WL PKKLEK LR+QG+ G  Y+LL G++K+L  +  EA +KPM LS  IVPR
Sbjct: 20   AWRILNWLWLKPKKLEKLLREQGLKGNSYRLLVGDIKDLLKIRKEATTKPMNLSDDIVPR 79

Query: 940  VNPFTLQTVQKHGKISLEWYGPTLRVNITDPDHIGEVLSNKHGHIFRAQSTPFLKLLVTG 761
            V  F  Q+V KHGK S  W+GPT RVNITDPD I +VL NK     +  + P ++LL  G
Sbjct: 80   VYSFVHQSVTKHGKNSFIWFGPTPRVNITDPDQIKDVL-NKIYDFQKPNANPLVRLLANG 138

Query: 760  LISYDGDKWVKHRKLINPAFHLEKLKIMLPAFYTSCIELVSKWQNLISKE-SCELDVWPE 584
            L+S +G++W KHRK+INPAFHLEKLKIMLP F+ SC +++SKW+ ++S + SCE+DVWP 
Sbjct: 139  LVSLEGEQWSKHRKIINPAFHLEKLKIMLPIFHKSCDDMISKWEKMLSSDGSCEMDVWPF 198

Query: 583  FQKLTSDVISRSAFGVSYVQGLRINQLESELAELLVQSSIQAPYIPGISW-YLPTKTNNR 407
             Q   SDVISR+AFG SY +G RI QL++E AEL  +  ++  YIPG  W +LPT T  R
Sbjct: 199  LQNFASDVISRAAFGSSYEEGKRIFQLQTEQAELTTKIMMKV-YIPG--WRFLPTATPRR 255

Query: 406  MKEIEAEVRTLLTNLVKQREEATKIGEVRVNDLLGLLLESNMKEIEDNGTSKSKGMSIDE 227
            MKEI+ +++  LT+++  RE A K GE   +DLLG+LLESN KE+E++G +K  GMS+D+
Sbjct: 256  MKEIDRDIKASLTDMINNRERALKAGEATKDDLLGILLESNHKEMEEHGNNKDVGMSLDD 315

Query: 226  IIDECKLFYIAGQETIATLLVWTTIVLSMHPEWQVKAREEVFEVFGKSKPDFEGLNRLKI 47
            +I+ECKLFY AGQET + LLVWT ++LS +P+WQ +AREEV +VFG +KPDF+GL+ LKI
Sbjct: 316  VIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEVLQVFGNNKPDFDGLSHLKI 375

Query: 46   VTMILYEVLRLYPPV 2
            VTMILYEVLRLYPPV
Sbjct: 376  VTMILYEVLRLYPPV 390


>dbj|BAL45206.1| cytochrome P450 monooxygenase [Glycyrrhiza uralensis]
          Length = 522

 Score =  427 bits (1098), Expect = e-117
 Identities = 207/375 (55%), Positives = 282/375 (75%), Gaps = 2/375 (0%)
 Frame = -2

Query: 1120 AWKIFNYIWLTPKKLEKYLRDQGISGPPYKLLTGNLKELGSMMMEAKSKPMELSHRIVPR 941
            AW++ N++WL PKKLE+ LR+QG+ G PY+L+ G+LK+L  M  EAKSKPM LS  I+PR
Sbjct: 24   AWRMLNWLWLRPKKLERLLREQGLQGNPYRLIVGDLKDLMKMRKEAKSKPMNLSDDILPR 83

Query: 940  VNPFTLQTVQKHGKISLEWYGPTLRVNITDPDHIGEVLSNKHGHIFRAQSTPFLKLLVTG 761
            V P+  Q+V KHGK S  W+GP  RV +TDP+ I +VL NK     ++     +KLL TG
Sbjct: 84   VFPYVQQSVTKHGKNSFIWFGPIPRVTLTDPEQIKDVL-NKIYDFPKSDGDRLVKLLATG 142

Query: 760  LISYDGDKWVKHRKLINPAFHLEKLKIMLPAFYTSCIELVSKWQNLISKE-SCELDVWPE 584
            L+SY+G+KW KHR++INPAF+LEKLKIMLP F+ SC +L++KW+ ++S + SCE+D+WP 
Sbjct: 143  LVSYEGEKWSKHRRIINPAFNLEKLKIMLPIFFQSCNDLITKWEGMLSSDGSCEMDIWPF 202

Query: 583  FQKLTSDVISRSAFGVSYVQGLRINQLESELAELLVQSSIQAPYIPGISW-YLPTKTNNR 407
             Q L SDVI+R+AFG SY +G RI QL+ E  E  +   ++  YIPG  W +LPT T+ R
Sbjct: 203  LQNLASDVIARAAFGSSYEEGRRIFQLQKEQVEHTMSVLVKV-YIPG--WRFLPTTTHRR 259

Query: 406  MKEIEAEVRTLLTNLVKQREEATKIGEVRVNDLLGLLLESNMKEIEDNGTSKSKGMSIDE 227
            MK+I+ E++  L  ++ +RE A K GE   N+LL +LLESN KE+E++G +++ GMS+++
Sbjct: 260  MKKIDREIKASLKEMINKRERALKAGEATKNNLLDILLESNHKEVEEHGNNRNVGMSLED 319

Query: 226  IIDECKLFYIAGQETIATLLVWTTIVLSMHPEWQVKAREEVFEVFGKSKPDFEGLNRLKI 47
            +I+ECKLFY AGQET + LLVWT ++LS +P+WQ +AREEV +VFG  KPDF+GL+ LKI
Sbjct: 320  VIEECKLFYFAGQETTSGLLVWTMVLLSRYPDWQARAREEVLQVFGNKKPDFDGLSHLKI 379

Query: 46   VTMILYEVLRLYPPV 2
            VTMILYEVLRLYPPV
Sbjct: 380  VTMILYEVLRLYPPV 394


>ref|XP_003546752.1| PREDICTED: secologanin synthase-like [Glycine max]
          Length = 520

 Score =  427 bits (1098), Expect = e-117
 Identities = 216/378 (57%), Positives = 277/378 (73%), Gaps = 4/378 (1%)
 Frame = -2

Query: 1123 CAWKIFNYIWLTPKKLEKYLRDQGISGPPYKLLTGNLKELGSMMMEAKSKPMELSHRIVP 944
            C+WK+ N++WL PK+LEK LR+QG  G PY L  G+ KE   M  EA SKPM LS  IVP
Sbjct: 19   CSWKVLNWLWLRPKRLEKLLREQGFRGNPYTLFFGDSKEFLKMRKEAVSKPMTLSDDIVP 78

Query: 943  RVNPFTLQTVQKHGKISLEWYGPTLRVNITDPDHIGEVLSNKHGHIFRAQSTPFLKLLVT 764
            RV+ +   +V KHGK S  W+GP  RV I DP+ I +V  NK+    +    P +KLL T
Sbjct: 79   RVSAYVQHSVNKHGKNSFIWFGPMPRVTILDPEQIKDVF-NKNYDFPKPNLNPLVKLLAT 137

Query: 763  GLISYDGDKWVKHRKLINPAFHLEKLKIMLPAFYTSCIELVSKWQNLISKE-SCELDVWP 587
            GL  Y+G+KW KHR++INPAF+LEKLKIMLP F  SC +LVSKW+ ++S E SCE+D WP
Sbjct: 138  GLAGYEGEKWSKHRRIINPAFNLEKLKIMLPLFLQSCNDLVSKWEGMLSSEGSCEMDAWP 197

Query: 586  EFQKLTSDVISRSAFGVSYVQGLRINQLESELAELLVQSSIQAPYIPGISW-YLPTKTNN 410
              Q LTSDVI+RSAFG SY +G RI QL+ E  E L++  ++   IPG  W +LPTKT+ 
Sbjct: 198  FLQNLTSDVIARSAFGSSYEEGRRIFQLQREQTEHLMKVILKIQ-IPG--WRFLPTKTHR 254

Query: 409  RMKEIEAEVRTLLTNLVKQREEATKIGEVRVNDLLGLLLESNMKEIEDNGTSKSK--GMS 236
            RMKEI+ E++  L N++ +RE+A K GE   NDLLG+LLESN KEI+++G   SK  GMS
Sbjct: 255  RMKEIDREIKASLKNMINKREKALKSGEATKNDLLGILLESNHKEIQEHGNRNSKNVGMS 314

Query: 235  IDEIIDECKLFYIAGQETIATLLVWTTIVLSMHPEWQVKAREEVFEVFGKSKPDFEGLNR 56
            ++++I+ECKLFY AGQET + LLVWT ++LS +P+WQ +AREE F+VFG  KPDF+GL+R
Sbjct: 315  LEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEAFQVFGYQKPDFDGLSR 374

Query: 55   LKIVTMILYEVLRLYPPV 2
            LKIVTMILYEVLRLYPP+
Sbjct: 375  LKIVTMILYEVLRLYPPL 392