BLASTX nr result
ID: Coptis25_contig00009021
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00009021
(1617 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002266768.1| PREDICTED: cytochrome P450 734A1 [Vitis vini... 486 e-139
ref|XP_002329227.1| cytochrome P450 [Populus trichocarpa] gi|222... 467 e-133
ref|XP_002515361.1| cytochrome P450, putative [Ricinus communis]... 454 e-129
ref|XP_002268401.1| PREDICTED: cytochrome P450 734A1-like [Vitis... 450 e-127
ref|XP_003552903.1| PREDICTED: cytochrome P450 734A1-like [Glyci... 449 e-127
>ref|XP_002266768.1| PREDICTED: cytochrome P450 734A1 [Vitis vinifera]
gi|297736650|emb|CBI25521.3| unnamed protein product
[Vitis vinifera]
Length = 525
Score = 486 bits (1251), Expect(2) = e-139
Identities = 242/468 (51%), Positives = 326/468 (69%), Gaps = 20/468 (4%)
Frame = -3
Query: 1546 LITIFFCFAFIL--FSTFLTHLYNILWLKPKRLREK----------PSFLYGNLMDITSI 1403
L+ C ++ F FL HLYN++W++P+RLR K PSF+YGN+ ++ I
Sbjct: 6 LVVKMLCSVLVVGVFCIFL-HLYNLMWVRPERLRGKLRRQGIRGPPPSFIYGNVPEMQKI 64
Query: 1402 QNSTAAKLKGQDGVHLLDNYFPVFYPYLEQWRRLYGNIFMYSMGPRVHLCVTRPDLVKDI 1223
Q A K + + +Y +PY EQWR+ YG ++ YS G R HL V P++VK++
Sbjct: 65 QLLMAMKPPNHAQI-VAHDYTSTLFPYFEQWRKAYGPVYTYSTGNRQHLYVNHPEMVKEM 123
Query: 1222 SQQKYMGLGKPSYLTNELGPILGNGIIRSNGHWWAQQKKIMAPEFFMDKVKGMVGLMVES 1043
+Q + LGKPSY+T L P+LGNGI+RSNG+ WA Q++I+APEFFMDKVKGMVGLM+ES
Sbjct: 124 NQSTSLELGKPSYVTKTLAPMLGNGILRSNGNTWAHQRRIVAPEFFMDKVKGMVGLMLES 183
Query: 1042 TITLLKSWENRIASEGGI-ADIKIGDDLRDLSADVISRACFGSSYGKGKEIFITLEALKQ 866
LL WE+ I + GG+ AD+ + +DLR SADVISR CFGSSYGKGK+IF L +L+Q
Sbjct: 184 AQPLLTKWEDCIQAHGGVSADVMVNEDLRSASADVISRVCFGSSYGKGKQIFSKLRSLQQ 243
Query: 865 VMSKKNILFGIPSFSYLPTKNNMKTWKLEIKPESLILNSVKQRRQES------TSSKDLL 704
+++ + LFG+PSF +L + LE + ESLI +VK+R QE +S KDL+
Sbjct: 244 LITNQAFLFGVPSFRFLWANKQKELKNLEREVESLIWKAVKEREQECLETSTLSSDKDLM 303
Query: 703 MIILENA-NSGLLTPGAAKRFIVDNCKSFYFAGYDTSAIAAQWCLMLLASHPEWQIRAHA 527
++LE+A N L ++KRFIVDNCKS YFAG++++A+AA WCLMLLA HP+WQ R A
Sbjct: 304 QLLLESAINDPNLGQASSKRFIVDNCKSIYFAGHESTAVAASWCLMLLALHPDWQARIRA 363
Query: 526 EVEEVCSGHLPNADSLVKMKLMTMVIQESLRLYPPTMFVAREVIEEVKLGDLRIPKGINI 347
E+ EVC ++P+ADS+ KMK MTMVIQE+LRLYPP FV+RE +EE+++G+ +PKG+ I
Sbjct: 364 EIAEVCGDNMPDADSITKMKWMTMVIQETLRLYPPAAFVSREALEEIQVGNFIVPKGVCI 423
Query: 346 WIPISTMHRDPAIWGEDANEFKPDRFANGISSACNLPNAYIPFGMGSR 203
W I T+HRD IWG DAN+FKP+RF NG+S AC P AYIPFG+G R
Sbjct: 424 WTLIPTLHRDTDIWGPDANQFKPERFENGVSKACKFPQAYIPFGLGPR 471
Score = 36.2 bits (82), Expect(2) = e-139
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = -2
Query: 206 KDLAMVEXXXXXXXXXXXXXXXXSPKYQHFPVNKMFVQPGYGVDLLIKRI 57
++ AMV+ SP Y+HFPV +M V+PG GV + I++I
Sbjct: 476 RNFAMVQLKIILSLIISKFTFTLSPTYRHFPVYRMIVEPGDGVHIRIRKI 525
>ref|XP_002329227.1| cytochrome P450 [Populus trichocarpa] gi|222871008|gb|EEF08139.1|
cytochrome P450 [Populus trichocarpa]
Length = 482
Score = 467 bits (1201), Expect(2) = e-133
Identities = 232/420 (55%), Positives = 303/420 (72%), Gaps = 6/420 (1%)
Frame = -3
Query: 1444 PSFLYGNLMDITSIQNSTAAKLKGQDGVHLLDNYFPVFYPYLEQWRRLYGNIFMYSMGPR 1265
PSFLYGNL ++ IQ +T Q + +Y +PY EQWR+ YG ++ YS G R
Sbjct: 9 PSFLYGNLPEMQKIQLNTLKASSFQAPDFIAHDYTSTVFPYFEQWRKEYGPVYTYSTGLR 68
Query: 1264 VHLCVTRPDLVKDISQQKYMGLGKPSYLTNELGPILGNGIIRSNGHWWAQQKKIMAPEFF 1085
HL V +P+LVK+++Q + LGKPSYLT + P+LGNGI+RSNG WAQQ+KI+APEF+
Sbjct: 69 QHLYVNQPELVKEMNQMISLDLGKPSYLTKRMAPLLGNGIVRSNGLVWAQQRKIVAPEFY 128
Query: 1084 MDKVKGMVGLMVESTITLLKSWENRIASEGGI-ADIKIGDDLRDLSADVISRACFGSSYG 908
MDKVKGMVGLMVES LLK WE I ++GGI AD+K+ +DLR+LSA+VISRACFGSSY
Sbjct: 129 MDKVKGMVGLMVESAQPLLKKWEECIEAQGGITADVKVDEDLRELSANVISRACFGSSYS 188
Query: 907 KGKEIFITLEALKQVMSKKNILFGIPSFSYLPTKNNMKTWKLEIKPESLILNSVKQRRQE 728
KGK+IF L +L+Q S +NILFG+ +F +LP K K LE + ESLI +VK+RRQE
Sbjct: 189 KGKQIFSKLRSLQQTFSNQNILFGVTNFGFLPVKKQNKISNLEREVESLIWEAVKERRQE 248
Query: 727 ----STSSKDLLMIILENA-NSGLLTPGAAKRFIVDNCKSFYFAGYDTSAIAAQWCLMLL 563
++S KDL++++LE A N L A+K F+VDNCK+ YFAG++++A+AA WCLMLL
Sbjct: 249 CQETNSSEKDLMLLLLEGAINDQSLGKDASKSFVVDNCKTIYFAGHESTAVAASWCLMLL 308
Query: 562 ASHPEWQIRAHAEVEEVCSGHLPNADSLVKMKLMTMVIQESLRLYPPTMFVAREVIEEVK 383
A HPEWQ E+ E+ L + DSL +K +TMVIQE+LRLYPP FV+RE +EE +
Sbjct: 309 ALHPEWQGGIRKELAEISKDGLLSVDSLHHLKTVTMVIQEALRLYPPAAFVSREALEETQ 368
Query: 382 LGDLRIPKGINIWIPISTMHRDPAIWGEDANEFKPDRFANGISSACNLPNAYIPFGMGSR 203
LG++ + KG+ +W I T+HRDPA+WG DANEFKP+RF G+S AC P AYIPFG+G R
Sbjct: 369 LGNIVVSKGVCLWTLIPTLHRDPAVWGSDANEFKPERFTWGVSKACKCPQAYIPFGVGPR 428
Score = 38.1 bits (87), Expect(2) = e-133
Identities = 20/50 (40%), Positives = 26/50 (52%)
Frame = -2
Query: 206 KDLAMVEXXXXXXXXXXXXXXXXSPKYQHFPVNKMFVQPGYGVDLLIKRI 57
K+ AMVE SPKY H P +M V+PG GV +LI++I
Sbjct: 433 KNFAMVELKVVLSLIVSKFSFSLSPKYHHSPAYRMIVEPGDGVQILIQKI 482
>ref|XP_002515361.1| cytochrome P450, putative [Ricinus communis]
gi|223545305|gb|EEF46810.1| cytochrome P450, putative
[Ricinus communis]
Length = 523
Score = 454 bits (1167), Expect(2) = e-129
Identities = 235/456 (51%), Positives = 316/456 (69%), Gaps = 18/456 (3%)
Frame = -3
Query: 1516 ILFSTFLTHLYNILWLKPKRLREK----------PSFLYGNLMDITSIQNSTAAKLKGQD 1367
+L F+ HLY + L +RL+ K PSFLYGNL ++ IQ +K Q+
Sbjct: 18 LLAVCFIIHLYKTVLLSSQRLKTKLHVQGIRGPSPSFLYGNLSEMQQIQLKA---MKAQN 74
Query: 1366 -GVHLLDNYFPVFYPYLEQWRRLYGNIFMYSMGPRVHLCVTRPDLVKDISQQKYMGLGKP 1190
G + +Y +PY E WR+ YG I+ YS G R HL V P++VK+++Q + LGKP
Sbjct: 75 HGEIVAHDYTSSIFPYFEYWRKQYGLIYTYSTGFRQHLYVNDPEVVKEMNQSINLDLGKP 134
Query: 1189 SYLTNELGPILGNGIIRSNGHWWAQQKKIMAPEFFMDKVKGMVGLMVESTITLLKSWENR 1010
+YLT L P+LGNGIIRSNGH WAQQ+KI+APEFF DKVKGMVGLM+ES L++ WE
Sbjct: 135 TYLTKRLEPMLGNGIIRSNGHLWAQQRKIIAPEFFKDKVKGMVGLMIESAEALVRKWEES 194
Query: 1009 I--ASEGGIADIKIGDDLRDLSADVISRACFGSSYGKGKEIFITLEALKQVMSKKNILFG 836
I A G ADI++ +DLR LSADVI+RACFGSSY KGK+IF L +++ +++ ++ILFG
Sbjct: 195 ITEAEGGSQADIRVDEDLRGLSADVIARACFGSSYSKGKQIFSKLRSIQNLLTNQSILFG 254
Query: 835 IPSFSYLPTKNNMKTWKLEIKPESLILNSVKQRR----QESTSSKDLLMIILENA-NSGL 671
+ SF + +KN+ LE + ESLI +VK+R ++++ KDL+ +LE A N G
Sbjct: 255 VTSFGFYASKNHKIITNLEREVESLIWETVKERERQCSEKASIEKDLMQQLLEEAVNDGE 314
Query: 670 LTPGAAKRFIVDNCKSFYFAGYDTSAIAAQWCLMLLASHPEWQIRAHAEVEEVCSGHLPN 491
T + KRFIVDNCKS YFAG++++A AA WCLMLLA HPEWQ R EV +VC L +
Sbjct: 315 ATKFSPKRFIVDNCKSIYFAGHESTATAASWCLMLLALHPEWQSRIREEVNQVCKDGL-D 373
Query: 490 ADSLVKMKLMTMVIQESLRLYPPTMFVAREVIEEVKLGDLRIPKGINIWIPISTMHRDPA 311
A+S+ +K++T+VIQE+LRLYPP FV+RE +EEV++G +PKG+ IW I T+HRDP
Sbjct: 374 ANSISNLKMVTIVIQEALRLYPPAAFVSREALEEVQIGKYTVPKGVCIWTLIPTLHRDPN 433
Query: 310 IWGEDANEFKPDRFANGISSACNLPNAYIPFGMGSR 203
IWG+DANEF+P+RFA+G+S AC AYIPFG+G+R
Sbjct: 434 IWGQDANEFRPERFADGVSKACKSAQAYIPFGVGTR 469
Score = 35.0 bits (79), Expect(2) = e-129
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = -2
Query: 134 PKYQHFPVNKMFVQPGYGVDLLIKRI 57
P YQH P +M V+P +GV +LIK++
Sbjct: 498 PNYQHSPAFRMIVEPEHGVQILIKKV 523
>ref|XP_002268401.1| PREDICTED: cytochrome P450 734A1-like [Vitis vinifera]
Length = 510
Score = 450 bits (1157), Expect(2) = e-127
Identities = 226/457 (49%), Positives = 299/457 (65%), Gaps = 10/457 (2%)
Frame = -3
Query: 1543 ITIFFCFAFILFSTFLTHLYNILWLKPKRLRE----------KPSFLYGNLMDITSIQNS 1394
+ +F A + LYN L +KPK+LR PSFL GN+ +I Q S
Sbjct: 6 VKLFISIALVGVLGLFFRLYNALVVKPKKLRSILRNQGISGPPPSFLLGNIREIKESQ-S 64
Query: 1393 TAAKLKGQDGVHLLDNYFPVFYPYLEQWRRLYGNIFMYSMGPRVHLCVTRPDLVKDISQQ 1214
TA K D N +P+ E+WR YG +FM+S+G L V +PD+VK+I+
Sbjct: 65 TAVK----DSCTDTHNCAAALFPFFEKWRNKYGQVFMFSLGNTQILHVNQPDIVKEITTC 120
Query: 1213 KYMGLGKPSYLTNELGPILGNGIIRSNGHWWAQQKKIMAPEFFMDKVKGMVGLMVESTIT 1034
+ GKPSY ELGP+LG GI+ SNG WA +KI+APE +MDKVKGM+GL+ ES T
Sbjct: 121 TSLDFGKPSYQHRELGPLLGQGILTSNGAVWAHNRKILAPELYMDKVKGMIGLITESVDT 180
Query: 1033 LLKSWENRIASEGGIADIKIGDDLRDLSADVISRACFGSSYGKGKEIFITLEALKQVMSK 854
LLK+W + I +EGGIADI+I + +R S DVISRACFGSSY KG+EIF+ L AL++ MSK
Sbjct: 181 LLKTWRSMIEAEGGIADIRIDEHMRSFSGDVISRACFGSSYTKGEEIFLRLRALQEAMSK 240
Query: 853 KNILFGIPSFSYLPTKNNMKTWKLEIKPESLILNSVKQRRQESTSSKDLLMIILENANSG 674
K I IP Y+PTK+N W+LE +LIL V R + + KDLL ++LE A +
Sbjct: 241 K-IFLDIPGMRYVPTKHNRDVWELEKDVRNLILKVVSGREELAAHEKDLLQMVLEGAKNS 299
Query: 673 LLTPGAAKRFIVDNCKSFYFAGYDTSAIAAQWCLMLLASHPEWQIRAHAEVEEVCSGHLP 494
L+ A FIVDNCK+ Y AGY+T+A++A+WCLMLLA++P+WQ R EV E+C G P
Sbjct: 300 DLSQEAIDNFIVDNCKNIYLAGYETTAVSAEWCLMLLAANPDWQARVREEVVEICKGRTP 359
Query: 493 NADSLVKMKLMTMVIQESLRLYPPTMFVAREVIEEVKLGDLRIPKGINIWIPISTMHRDP 314
+AD + KMK MTMVI ESLRLYPP V+RE + ++K G + +PKG+N+W + T+H DP
Sbjct: 360 DADMIRKMKQMTMVIHESLRLYPPVAVVSREALADMKFGGIHVPKGVNVWSLVVTLHTDP 419
Query: 313 AIWGEDANEFKPDRFANGISSACNLPNAYIPFGMGSR 203
WG DA +F P+RFANGI+ AC LP+ Y+PFG+G R
Sbjct: 420 ENWGPDALKFNPERFANGITGACKLPHLYMPFGVGPR 456
Score = 35.0 bits (79), Expect(2) = e-127
Identities = 17/50 (34%), Positives = 27/50 (54%)
Frame = -2
Query: 206 KDLAMVEXXXXXXXXXXXXXXXXSPKYQHFPVNKMFVQPGYGVDLLIKRI 57
++LAMVE SP Y+H P ++ ++P GVDLL+K++
Sbjct: 461 QNLAMVELKILISLILSNFSFSLSPNYKHSPALRLVIEPENGVDLLVKKL 510
>ref|XP_003552903.1| PREDICTED: cytochrome P450 734A1-like [Glycine max]
Length = 514
Score = 449 bits (1155), Expect(2) = e-127
Identities = 218/454 (48%), Positives = 305/454 (67%), Gaps = 10/454 (2%)
Frame = -3
Query: 1534 FFCFAFILFSTFLTHLYNILWLKPKRLREK----------PSFLYGNLMDITSIQNSTAA 1385
F F+ F L LY L P RLR K P+ L GN++DI +++T+
Sbjct: 10 FMSIVFLGFVGLLCRLYTSLVENPNRLRSKLMKQGISGPPPTILLGNIVDIKKARSTTSN 69
Query: 1384 KLKGQDGVHLLDNYFPVFYPYLEQWRRLYGNIFMYSMGPRVHLCVTRPDLVKDISQQKYM 1205
+ + + N V P ++W+ YG +FM+S+G R LCV++PD+V+DI+ +
Sbjct: 70 SPSFE--IPVSHNCASVILPLFDKWKEQYGQVFMFSLGNRQILCVSQPDIVRDITTCTSL 127
Query: 1204 GLGKPSYLTNELGPILGNGIIRSNGHWWAQQKKIMAPEFFMDKVKGMVGLMVESTITLLK 1025
LGKPSY +LGP+LG G++ SNG W Q+KI+APE +M+KVKGM+ ++ ES I+LL
Sbjct: 128 DLGKPSYQQKQLGPLLGQGVLTSNGTTWVHQRKILAPELYMEKVKGMMNIISESAISLLN 187
Query: 1024 SWENRIASEGGIADIKIGDDLRDLSADVISRACFGSSYGKGKEIFITLEALKQVMSKKNI 845
W++R +EGG+ADIKI + +R+ S DVISRACFGS+Y KG+EIF+ L AL+++MS KN+
Sbjct: 188 LWKSRTEAEGGVADIKIDEYMRNFSGDVISRACFGSNYSKGEEIFLKLGALQEIMSWKNV 247
Query: 844 LFGIPSFSYLPTKNNMKTWKLEIKPESLILNSVKQRRQESTSSKDLLMIILENANSGLLT 665
GIP YLPTK N + WKLE + + LIL VK+R+ E++ K LL ++LE A + +
Sbjct: 248 SIGIPGMRYLPTKTNREAWKLEKEVKKLILQGVKERK-ETSFEKHLLQMVLEGARNSNTS 306
Query: 664 PGAAKRFIVDNCKSFYFAGYDTSAIAAQWCLMLLASHPEWQIRAHAEVEEVCSGHLPNAD 485
A RFIVDNCK+ Y AGY+T+A+AA WCLMLLAS+ W R EV E+C G +P+ +
Sbjct: 307 QEAIDRFIVDNCKNIYLAGYETTAVAATWCLMLLASNQNWHDRVRTEVLEICRGSIPDFN 366
Query: 484 SLVKMKLMTMVIQESLRLYPPTMFVAREVIEEVKLGDLRIPKGINIWIPISTMHRDPAIW 305
L KMK +TMVI ESLRLYPP V+R+ +++K G++ +PKG N+WI + T+H DP IW
Sbjct: 367 MLCKMKQLTMVIHESLRLYPPVAVVSRQAFKDMKFGNIDVPKGFNLWIMVVTLHTDPDIW 426
Query: 304 GEDANEFKPDRFANGISSACNLPNAYIPFGMGSR 203
G+DAN+F P+RFANG AC LP+ Y+PFG+G R
Sbjct: 427 GDDANKFNPERFANGTIGACKLPHMYMPFGVGPR 460
Score = 33.9 bits (76), Expect(2) = e-127
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = -2
Query: 206 KDLAMVEXXXXXXXXXXXXXXXXSPKYQHFPVNKMFVQPGYGVDLLIKRI 57
++LAMVE SP+Y H P ++ ++P +GV LL+K++
Sbjct: 465 QNLAMVELKMLVALILSKFTFSLSPRYVHSPTLRLLIEPEHGVHLLVKKL 514