BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00008485
         (2389 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267958.1| PREDICTED: 3-epi-6-deoxocathasterone 23-mono...   587   e-165
ref|XP_002310278.1| predicted protein [Populus trichocarpa] gi|2...   585   e-164
emb|CAN73509.1| hypothetical protein VITISV_007910 [Vitis vinifera]   575   e-161
ref|NP_568002.1| 3-epi-6-deoxocathasterone 23-monooxygenase [Ara...   560   e-157
emb|CAB16850.1| cytochrome P450 like protein [Arabidopsis thalia...   560   e-157

>ref|XP_002267958.1| PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Vitis
            vinifera] gi|297735073|emb|CBI17435.3| unnamed protein
            product [Vitis vinifera]
          Length = 486

 Score =  587 bits (1512), Expect = e-165
 Identities = 285/446 (63%), Positives = 356/446 (79%), Gaps = 10/446 (2%)
 Frame = +3

Query: 726  GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 905
            GWP IGETL FIASG+SS+P+SFM+KRKS YGKVFK+++LG P+IVS DPEVNKVVLQN 
Sbjct: 40   GWPVIGETLDFIASGWSSRPVSFMEKRKSRYGKVFKTNILGKPVIVSTDPEVNKVVLQNI 99

Query: 906  GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1085
            GN FIP YP++I E+ G+SSILQM+G++ K+VH L+GGFL+SPQLKARIT++IER V ++
Sbjct: 100  GNVFIPAYPQTITEILGESSILQMNGSLQKRVHALLGGFLRSPQLKARITRDIERYVKLT 159

Query: 1086 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1265
            ++SW+D+ ++Y+Q+E + ITF VLV+VL+ + PGEDL F+KREF E IKGL+ LPIKLPG
Sbjct: 160  LDSWKDQHIVYVQDEVRKITFDVLVRVLVSITPGEDLNFMKREFAEVIKGLICLPIKLPG 219

Query: 1266 TRXXXXXXXXXXXXXXVNRIVEEKKLKM-----EVMTGDVMDAMEMLLSDAGD-----YI 1415
            TR              V RIVEE+K  M     E + G   DA+++LL D GD      +
Sbjct: 220  TRLYKSLKAKERLLKLVRRIVEERKAAMDRGDHETLKGPPNDAVDVLLRDTGDSSETSRL 279

Query: 1416 PFDSIGENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGEDYL 1595
              D I  N++EMMIPGEE++P  MTLA+K+LSDCP+AL QL EENM+LK  K   GEDY 
Sbjct: 280  SLDFISSNMIEMMIPGEETLPTAMTLAVKFLSDCPVALNQLMEENMELKKGKALSGEDYA 339

Query: 1596 WTDYMSLPFTQNVISETLRIANIINAVWRKAIKDVEIKGYLIPKGWCMQAYFSSVHMDED 1775
            WTDYM LPFTQNVI+ETLR+ANIINAVWRKA+KDV+IK YLIP+GW + A F+S+HMDE+
Sbjct: 340  WTDYMCLPFTQNVINETLRMANIINAVWRKAVKDVKIKDYLIPEGWGVMASFTSIHMDEE 399

Query: 1776 NYENPYQFDPWRWQTKAGHVNNYNTCFTPFGGGQRLCPGLEFSRLEVSIFLHHFVTSYRW 1955
            NYENPYQF+PWRW+ KA  VNN N  +TPFGGGQRLCPGLE S+LE+SIFLHH VT+YRW
Sbjct: 400  NYENPYQFNPWRWE-KAASVNN-NNSYTPFGGGQRLCPGLELSKLEISIFLHHLVTTYRW 457

Query: 1956 VAEEDSIVSFPTVKMKRKLPIRVTPV 2033
            VA++D +V FPTVKM++KLPI VTP+
Sbjct: 458  VAKKDDVVYFPTVKMRKKLPITVTPL 483


>ref|XP_002310278.1| predicted protein [Populus trichocarpa] gi|222853181|gb|EEE90728.1|
            predicted protein [Populus trichocarpa]
          Length = 463

 Score =  585 bits (1508), Expect = e-164
 Identities = 285/448 (63%), Positives = 347/448 (77%), Gaps = 12/448 (2%)
 Frame = +3

Query: 726  GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 905
            GWP IGETL FIA+GY+SQP+SFM+KR+SLYGKVFK+H+LG PIIVS DP VNKVVLQNH
Sbjct: 14   GWPLIGETLDFIAAGYTSQPVSFMEKRRSLYGKVFKTHILGTPIIVSTDPAVNKVVLQNH 73

Query: 906  GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1085
            GN FIP YPKS++E+FG+ SILQ +G + KKVH LIGGFL+SPQ K RITK+IE  V ++
Sbjct: 74   GNIFIPAYPKSVREIFGEYSILQTNGTLQKKVHALIGGFLRSPQFKTRITKDIEHHVKLT 133

Query: 1086 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1265
            + SW+D  L+++QEET+  TF VLVK L+ +DP +DL+FLKREF EFIKGL+ LPIKLPG
Sbjct: 134  LTSWKDLPLLFVQEETQKFTFKVLVKALLSLDPSDDLDFLKREFDEFIKGLICLPIKLPG 193

Query: 1266 TRXXXXXXXXXXXXXXVNRIVEEKKLKMEVMTGD------VMDAMEMLLSDAG------D 1409
            TR              V +IVE++KL +E             DA+++LL D         
Sbjct: 194  TRLYKSLKAKERIFKLVKKIVEQRKLGLEKSEDQEHQNTIPNDAVDVLLRDISGEQNDKQ 253

Query: 1410 YIPFDSIGENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGED 1589
             +  D I  +I+E+MIPGEE+VPM MTLA+K+LSDCP+AL+ L EENM LK QK   G+D
Sbjct: 254  SLSLDFIISHIIELMIPGEETVPMSMTLAVKFLSDCPVALELLTEENMKLKRQKTDSGDD 313

Query: 1590 YLWTDYMSLPFTQNVISETLRIANIINAVWRKAIKDVEIKGYLIPKGWCMQAYFSSVHMD 1769
            Y WTDYMSL FTQNVI+ETLR+ANIINAVWRKA+KDVEIKG LIP+GWC+   FSSVHMD
Sbjct: 314  YCWTDYMSLQFTQNVINETLRVANIINAVWRKALKDVEIKGILIPEGWCVLTSFSSVHMD 373

Query: 1770 EDNYENPYQFDPWRWQTKAGHVNNYNTCFTPFGGGQRLCPGLEFSRLEVSIFLHHFVTSY 1949
            E+NYENP++FDPWRW+     V   N CFTPFGGGQRLCPGLE SRLE+SIFLHH VT+Y
Sbjct: 374  EENYENPHEFDPWRWEKTGASVK--NNCFTPFGGGQRLCPGLELSRLEISIFLHHLVTTY 431

Query: 1950 RWVAEEDSIVSFPTVKMKRKLPIRVTPV 2033
            RW+AE+D I+ FPTVK+K+KLPIRVT +
Sbjct: 432  RWIAEKDDIIYFPTVKLKKKLPIRVTSI 459


>emb|CAN73509.1| hypothetical protein VITISV_007910 [Vitis vinifera]
          Length = 501

 Score =  575 bits (1481), Expect = e-161
 Identities = 284/461 (61%), Positives = 355/461 (77%), Gaps = 25/461 (5%)
 Frame = +3

Query: 726  GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 905
            GWP IGETL FIASG+SS+P+SFM+KRKS YGKVFK+++LG P+IVS DPEVNKVVLQN 
Sbjct: 40   GWPVIGETLDFIASGWSSRPVSFMEKRKSRYGKVFKTNILGKPVIVSTDPEVNKVVLQNI 99

Query: 906  GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1085
            GN FIP YP++I E+ G+SSILQM+G++ K+VH L+GGFL+SPQLKARIT++IER V ++
Sbjct: 100  GNXFIPAYPQTITEILGESSILQMNGSLQKRVHALLGGFLRSPQLKARITRDIERYVKLT 159

Query: 1086 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1265
            ++SW+D+ ++Y+Q+E + ITF VLV+VL+ + PGEDL F+KREF E IKGL+ LPIKLPG
Sbjct: 160  LDSWKDQHIVYVQDEVRKITFDVLVRVLVSITPGEDLNFMKREFAEVIKGLICLPIKLPG 219

Query: 1266 TRXXXXXXXXXXXXXXVNRIVEEKKLKM-----EVMTGDVMDAMEMLLSDAGD-----YI 1415
            TR              V RIVEE+K  M     E + G   DA+++LL D GD      +
Sbjct: 220  TRLYKSLKAKERLLKLVRRIVEERKAAMDRGDHETLKGPPNDAVDVLLRDTGDSSETSRL 279

Query: 1416 PFDSIGENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGEDYL 1595
              D I  N++EMMIPGEE++P  MTLA+K+LSDCP+AL QL EENM+LK  K   GEDY 
Sbjct: 280  SLDFISSNMIEMMIPGEETLPTAMTLAVKFLSDCPVALNQLMEENMELKKGKALSGEDYA 339

Query: 1596 WTDYMSLPFTQN---------------VISETLRIANIINAVWRKAIKDVEIKGYLIPKG 1730
            WTDYM LPFTQN               VI+ETLR+ANIINAVWRKA+KDV+IK YLIP+G
Sbjct: 340  WTDYMCLPFTQNVGSSLNMRHCEVEWKVINETLRMANIINAVWRKAVKDVKIKDYLIPEG 399

Query: 1731 WCMQAYFSSVHMDEDNYENPYQFDPWRWQTKAGHVNNYNTCFTPFGGGQRLCPGLEFSRL 1910
            W + A F+S+HMDE+NYENPYQF+PWRW+ KA  VNN N  +TPFGGGQRLCPGLE S+L
Sbjct: 400  WGVMASFTSIHMDEENYENPYQFNPWRWE-KAASVNN-NNSYTPFGGGQRLCPGLELSKL 457

Query: 1911 EVSIFLHHFVTSYRWVAEEDSIVSFPTVKMKRKLPIRVTPV 2033
            E+SIFLHH VT+Y WVA++D +V FPTVKM++KLPI VTP+
Sbjct: 458  EISIFLHHLVTTYXWVAKKDDVVYFPTVKMRKKLPITVTPL 498


>ref|NP_568002.1| 3-epi-6-deoxocathasterone 23-monooxygenase [Arabidopsis thaliana]
            gi|150421525|sp|Q9M066.3|C90C1_ARATH RecName:
            Full=3-epi-6-deoxocathasterone 23-monooxygenase; AltName:
            Full=Cytochrome P450 90C1; AltName: Full=Protein
            ROTUNDIFOLIA 3 gi|115646893|gb|ABJ17155.1| At4g36380
            [Arabidopsis thaliana] gi|332661249|gb|AEE86649.1|
            3-epi-6-deoxocathasterone 23-monooxygenase [Arabidopsis
            thaliana]
          Length = 524

 Score =  560 bits (1443), Expect = e-157
 Identities = 275/440 (62%), Positives = 336/440 (76%), Gaps = 4/440 (0%)
 Frame = +3

Query: 726  GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 905
            GWP IGETL+FIA GYSS+P++FMDKRKSLYGKVFK++++G PII+S D EVNKVVLQNH
Sbjct: 75   GWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNKVVLQNH 134

Query: 906  GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1085
            GN+F+P YPKSI EL G++SIL ++G   K++H LIG FL+SP LK RIT++IE  V ++
Sbjct: 135  GNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIEASVVLT 194

Query: 1086 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1265
            + SW    L+++Q+E K +TF +LVKVLM   PGED+  LK EF EFIKGL+ +PIK PG
Sbjct: 195  LASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICIPIKFPG 254

Query: 1266 TRXXXXXXXXXXXXXXVNRIVEEKKLKMEVMTGDVMDAMEMLLSDAGD----YIPFDSIG 1433
            TR              V ++VEE+++ M   T    D +++LL D GD      P D + 
Sbjct: 255  TRLYKSLKAKERLIKMVKKVVEERQVAM-TTTSPANDVVDVLLRDGGDSEKQSQPSDFVS 313

Query: 1434 ENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGEDYLWTDYMS 1613
              IVEMMIPGEE++P  MTLA+K+LSD P+AL +L EENM++K +K  LGE+Y WTDYMS
Sbjct: 314  GKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKWTDYMS 373

Query: 1614 LPFTQNVISETLRIANIINAVWRKAIKDVEIKGYLIPKGWCMQAYFSSVHMDEDNYENPY 1793
            L FTQNVI+ETLR+ANIIN VWRKA+KDVEIKGYLIPKGWC+ A F SVHMDED Y+NPY
Sbjct: 374  LSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPY 433

Query: 1794 QFDPWRWQTKAGHVNNYNTCFTPFGGGQRLCPGLEFSRLEVSIFLHHFVTSYRWVAEEDS 1973
            QFDPWRW    G  N+ + CFTPFGGGQRLCPGLE S+LE+SIFLHH VT Y W AEED 
Sbjct: 434  QFDPWRWDRINGSANS-SICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWTAEEDE 492

Query: 1974 IVSFPTVKMKRKLPIRVTPV 2033
            IVSFPTVKMKR+LPIRV  V
Sbjct: 493  IVSFPTVKMKRRLPIRVATV 512


>emb|CAB16850.1| cytochrome P450 like protein [Arabidopsis thaliana]
            gi|7270586|emb|CAB80304.1| cytochrome P450 like protein
            [Arabidopsis thaliana]
          Length = 457

 Score =  560 bits (1443), Expect = e-157
 Identities = 275/440 (62%), Positives = 336/440 (76%), Gaps = 4/440 (0%)
 Frame = +3

Query: 726  GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 905
            GWP IGETL+FIA GYSS+P++FMDKRKSLYGKVFK++++G PII+S D EVNKVVLQNH
Sbjct: 8    GWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNKVVLQNH 67

Query: 906  GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1085
            GN+F+P YPKSI EL G++SIL ++G   K++H LIG FL+SP LK RIT++IE  V ++
Sbjct: 68   GNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIEASVVLT 127

Query: 1086 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1265
            + SW    L+++Q+E K +TF +LVKVLM   PGED+  LK EF EFIKGL+ +PIK PG
Sbjct: 128  LASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICIPIKFPG 187

Query: 1266 TRXXXXXXXXXXXXXXVNRIVEEKKLKMEVMTGDVMDAMEMLLSDAGD----YIPFDSIG 1433
            TR              V ++VEE+++ M   T    D +++LL D GD      P D + 
Sbjct: 188  TRLYKSLKAKERLIKMVKKVVEERQVAM-TTTSPANDVVDVLLRDGGDSEKQSQPSDFVS 246

Query: 1434 ENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGEDYLWTDYMS 1613
              IVEMMIPGEE++P  MTLA+K+LSD P+AL +L EENM++K +K  LGE+Y WTDYMS
Sbjct: 247  GKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKWTDYMS 306

Query: 1614 LPFTQNVISETLRIANIINAVWRKAIKDVEIKGYLIPKGWCMQAYFSSVHMDEDNYENPY 1793
            L FTQNVI+ETLR+ANIIN VWRKA+KDVEIKGYLIPKGWC+ A F SVHMDED Y+NPY
Sbjct: 307  LSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPY 366

Query: 1794 QFDPWRWQTKAGHVNNYNTCFTPFGGGQRLCPGLEFSRLEVSIFLHHFVTSYRWVAEEDS 1973
            QFDPWRW    G  N+ + CFTPFGGGQRLCPGLE S+LE+SIFLHH VT Y W AEED 
Sbjct: 367  QFDPWRWDRINGSANS-SICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWTAEEDE 425

Query: 1974 IVSFPTVKMKRKLPIRVTPV 2033
            IVSFPTVKMKR+LPIRV  V
Sbjct: 426  IVSFPTVKMKRRLPIRVATV 445