BLASTX nr result
ID: Coptis25_contig00008123
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00008123
(1941 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAR06921.1| UDP-glycosyltransferase 89B2 [Stevia rebaudiana] 508 e-154
ref|XP_002514595.1| UDP-glucosyltransferase, putative [Ricinus c... 511 e-153
ref|XP_002318584.1| predicted protein [Populus trichocarpa] gi|2... 483 e-145
gb|AFJ53022.1| UDP-glycosyltransferase 1 [Linum usitatissimum] 467 e-138
gb|AFJ53023.1| UDP-glycosyltransferase 1 [Linum usitatissimum] 463 e-135
>gb|AAR06921.1| UDP-glycosyltransferase 89B2 [Stevia rebaudiana]
Length = 468
Score = 508 bits (1307), Expect(2) = e-154
Identities = 252/431 (58%), Positives = 315/431 (73%), Gaps = 2/431 (0%)
Frame = +2
Query: 281 ILVTPKNLPILNPLLSTHPT-IQTLVLPFPIHPSIPPGVENTKDLPNGSFRTMMRVLAEL 457
ILVTPKNLP ++PLL+ HPT + L+LP P HP+IP G+EN KDLPN +F+ MM L +L
Sbjct: 42 ILVTPKNLPTISPLLAAHPTTVSALLLPLPPHPAIPSGIENVKDLPNDAFKAMMVALGDL 101
Query: 458 SYPITQWFNSHPSPPVAILSDMFLGWTHQLAIQLGIKRINFSPSGAFAISLFDCLWRDLP 637
P+ WF + P+PPVAI+SD FLGWTH LA++LGI+R FSPSGA A+S+ LWR P
Sbjct: 102 YNPLRDWFRNQPNPPVAIISDFFLGWTHHLAVELGIRRYTFSPSGALALSVIFSLWRYQP 161
Query: 638 KRNDPNDENELIXXXXXXXXXXXXWYQLSNLFRTYVKGDPDSEFTRDGLCANMVSWGVVF 817
KR D +E E I W+QLS ++R+YV+GDPDSEF +DG A++ SWG+V
Sbjct: 162 KRIDVENEKEAIKFPKIPNSPEYPWWQLSPIYRSYVEGDPDSEFIKDGFLADIASWGIVI 221
Query: 818 NSFNELESVYLDHIKNDLGRKRVFAVGPLVPPIDAPKERGGSSSIKVNDLLGWLDTCSDR 997
NSF ELE VY+DH+K++LG +VFAVGPL+PP D RGGSSS ND+L WLDTC+DR
Sbjct: 222 NSFTELEQVYVDHLKHELGHDQVFAVGPLLPPGDKTSGRGGSSS---NDVLSWLDTCADR 278
Query: 998 SVVYVCFGSQVVLNNRQMEELALGLEKSGTRFVWSVKEATTGHVAGEYGVVPSGFEDRVA 1177
+VVYVCFGSQ+VL N QME +ALGLEKS +FVWSVKE T GH A YG VP GFEDRV+
Sbjct: 279 TVVYVCFGSQMVLTNGQMEVVALGLEKSRVKFVWSVKEPTVGHEAANYGRVPPGFEDRVS 338
Query: 1178 GRGFVIKGWAPQVVILNHRAVGSFLTHCGWNSLLEAIIAGVPMLTWPMGADQYINSELLV 1357
GRG VI+GW PQV IL+H +VG FLTHCGWNS++EA+ A V MLTWPM ADQ+ N+ LL
Sbjct: 339 GRGLVIRGWVPQVAILSHDSVGVFLTHCGWNSVMEAVAAEVLMLTWPMSADQFSNATLLH 398
Query: 1358 ERIGVAVKVCQGGLTVPNADELALKFSESVG-ENQLLSERAKELSQAALNAVKDGGSSSR 1534
E + V +KVC+G VPN+DELA FS+S+ E +L +R KE +++A AV GSS
Sbjct: 399 E-LKVGIKVCEGSNIVPNSDELAELFSKSLSDETRLERKRVKEFAKSAKEAVGPKGSSVG 457
Query: 1535 DLDGLVRELQL 1567
+L+ LV L L
Sbjct: 458 ELERLVDNLSL 468
Score = 65.1 bits (157), Expect(2) = e-154
Identities = 30/35 (85%), Positives = 32/35 (91%)
Frame = +1
Query: 88 THILVFPYPAQGHMIALLDLTHQLVIRGLTITILV 192
+HILVFPYPAQGHM+ LLDLTHQL IR LTITILV
Sbjct: 10 SHILVFPYPAQGHMLTLLDLTHQLAIRNLTITILV 44
>ref|XP_002514595.1| UDP-glucosyltransferase, putative [Ricinus communis]
gi|223546199|gb|EEF47701.1| UDP-glucosyltransferase,
putative [Ricinus communis]
Length = 472
Score = 511 bits (1316), Expect(2) = e-153
Identities = 250/437 (57%), Positives = 314/437 (71%), Gaps = 4/437 (0%)
Frame = +2
Query: 281 ILVTPKNLPILNPLLSTHPTIQTLVLPFPIHPSIPPGVENTKDLPNGSFRTMMRVLAELS 460
ILVTPKNL L+PLLSTHP+I+TLV PFP HP IP GVEN KDLP ++R L L
Sbjct: 42 ILVTPKNLSFLHPLLSTHPSIETLVFPFPAHPLIPSGVENNKDLPAECTPVLIRALGGLY 101
Query: 461 YPITQWFNSHPSPPVAILSDMFLGWTHQLAIQLGIKRINFSPSGAFAISLFDCLWRDLPK 640
P+ WF SHPSPPVAI+SDMFLGWT LA QL I+RI FSPSGA A+S+ LWRD+P+
Sbjct: 102 DPLLHWFISHPSPPVAIISDMFLGWTQNLASQLNIRRIVFSPSGAMALSIIYSLWRDMPR 161
Query: 641 RNDPNDENELIXXXXXXXXXXXXWYQLSNLFRTYVKGDPDSEFTRDGLCANMVSWGVVFN 820
RN +NE++ W Q+S ++R+Y++ D + EF +D AN+VSWG+V N
Sbjct: 162 RN----QNEVVSFSRIPNCPNYPWRQISPIYRSYIENDTNWEFIKDSFRANLVSWGLVVN 217
Query: 821 SFNELESVYLDHIKNDLGRKRVFAVGPLVPP----IDAPKERGGSSSIKVNDLLGWLDTC 988
SF ELE +YLD+ K +LG V+AVGPL+PP I ERGG SS+ V+D++ WLDTC
Sbjct: 218 SFTELEEIYLDYFKKELGSDHVWAVGPLLPPHHDSISRQSERGGPSSVPVHDVMAWLDTC 277
Query: 989 SDRSVVYVCFGSQVVLNNRQMEELALGLEKSGTRFVWSVKEATTGHVAGEYGVVPSGFED 1168
D VVYVCFGSQ L Q+EELAL LE S F+W VKE H+ G+Y V+PSGFED
Sbjct: 278 EDHRVVYVCFGSQTWLTKDQIEELALSLEMSKVNFIWCVKE----HINGKYSVIPSGFED 333
Query: 1169 RVAGRGFVIKGWAPQVVILNHRAVGSFLTHCGWNSLLEAIIAGVPMLTWPMGADQYINSE 1348
RVAGRG VI+GW PQV+IL+H AVG+FLTHCGWNS+LE ++A VPML WPMGADQ++N+
Sbjct: 334 RVAGRGLVIRGWVPQVLILSHPAVGAFLTHCGWNSVLEGLVAAVPMLAWPMGADQFVNAR 393
Query: 1349 LLVERIGVAVKVCQGGLTVPNADELALKFSESVGENQLLSERAKELSQAALNAVKDGGSS 1528
LLV+ + VAV+VC+G TVPN+DELA ESV EN++ E+AK+L + A++ +KD G S
Sbjct: 394 LLVDELQVAVRVCEGAKTVPNSDELARVIMESVSENRVEREQAKKLRRVAMDTIKDRGRS 453
Query: 1529 SRDLDGLVRELQLLSVE 1579
+D DGLV+ L L VE
Sbjct: 454 MKDFDGLVKNLFRLKVE 470
Score = 60.8 bits (146), Expect(2) = e-153
Identities = 28/35 (80%), Positives = 31/35 (88%)
Frame = +1
Query: 88 THILVFPYPAQGHMIALLDLTHQLVIRGLTITILV 192
THILVFP+PAQGHMI LLDLT +L + GLTITILV
Sbjct: 10 THILVFPFPAQGHMIPLLDLTRKLAVHGLTITILV 44
>ref|XP_002318584.1| predicted protein [Populus trichocarpa] gi|222859257|gb|EEE96804.1|
predicted protein [Populus trichocarpa]
Length = 472
Score = 483 bits (1243), Expect(2) = e-145
Identities = 244/438 (55%), Positives = 312/438 (71%), Gaps = 5/438 (1%)
Frame = +2
Query: 281 ILVTPKNLPILNPLLSTHPTIQTLVLPFPIHPSIPPGVENTKDLP-NGSFRTMMRVLAEL 457
ILVTPKNLPILNPLLS + TI TLVLPFP +PSIP G+EN KDLP N +M+ L EL
Sbjct: 37 ILVTPKNLPILNPLLSKNSTINTLVLPFPNYPSIPLGIENLKDLPPNIRPTSMIHALGEL 96
Query: 458 SYPITQWFNSHPSPPVAILSDMFLGWTHQLAIQLGIKRINFSPSGAFAISLFDCLWRDLP 637
P+ WF SHPSPPVAI+SDMFLGWTH+LA QLG++R FSPSGA A++ LW+++P
Sbjct: 97 YQPLLSWFRSHPSPPVAIISDMFLGWTHRLACQLGVRRFVFSPSGAMALATMYSLWQEMP 156
Query: 638 KRNDPNDENELIXXXXXXXXXXXXWYQLSNLFRTYVKGDPDSEFTRDGLCANMVSWGVVF 817
N P D+NEL W Q+S ++R+YV+GDP SEFT++G+ AN+ SWG++
Sbjct: 157 --NAPKDQNELFSFSKIPSCPKYPWLQISTIYRSYVEGDPVSEFTKEGMEANIASWGLIV 214
Query: 818 NSFNELESVYLDHIKNDLGRKRVFAVGPLVPP--ID-APKERGGSSSIKVNDLLGWLDTC 988
NS LE +Y +H++ LG RV+AVGP++P ID P ERG + ++DL WLDTC
Sbjct: 215 NSLTLLEGIYFEHLRKQLGHDRVWAVGPILPEKTIDMTPPERG----VSMHDLKTWLDTC 270
Query: 989 SDRSVVYVCFGSQVVLNNRQMEELALGLEKSGTRFVWSVKEATTGHVAGEYGVVPSGFED 1168
D VVYVC+G+QVVL QME +A GLEKSG F+W VK+ + HV Y ++PSGFED
Sbjct: 271 EDHKVVYVCYGTQVVLTKYQMEAVASGLEKSGVHFIWCVKQPSKEHVGEGYSMIPSGFED 330
Query: 1169 RVAGRGFVIKGWAPQVVILNHRAVGSFLTHCGWNSLLEAIIAGVPMLTWPMGADQYINSE 1348
RVAGRG +I+GWAPQV IL+HRAVG+FLTHCGWNS+LE I+AGVPML PM ADQ++ +
Sbjct: 331 RVAGRGLIIRGWAPQVWILSHRAVGAFLTHCGWNSILEGIVAGVPMLACPMAADQFVGAT 390
Query: 1349 LLVERIGVAVKVCQGGLTVPNADELALKFSESVG-ENQLLSERAKELSQAALNAVKDGGS 1525
LLVE + VA +VC G V N+ +LA ESV E+Q+ ERAKEL AAL+A+K+ GS
Sbjct: 391 LLVEDLKVAKRVCDGANLVSNSAKLARTLMESVSDESQVEKERAKELRMAALDAIKEDGS 450
Query: 1526 SSRDLDGLVRELQLLSVE 1579
S + L+ V+ + L +E
Sbjct: 451 SDKHLNAFVKHVVGLGME 468
Score = 61.2 bits (147), Expect(2) = e-145
Identities = 27/39 (69%), Positives = 33/39 (84%)
Frame = +1
Query: 76 LTNSTHILVFPYPAQGHMIALLDLTHQLVIRGLTITILV 192
++ H+L+FP+PAQGH+I LLDL H LVIRGLTITILV
Sbjct: 1 MSAGAHVLLFPFPAQGHLIPLLDLAHHLVIRGLTITILV 39
>gb|AFJ53022.1| UDP-glycosyltransferase 1 [Linum usitatissimum]
Length = 476
Score = 467 bits (1202), Expect(2) = e-138
Identities = 240/432 (55%), Positives = 305/432 (70%), Gaps = 5/432 (1%)
Frame = +2
Query: 281 ILVTPKNLPILNPLLSTHPTIQTLVLPFPIHPSIPPGVENTKDLPNGSFRT----MMRVL 448
ILVTPKNLP+L PLLS HP+IQ L LPFP P IPPGVENTKDLP S ++ M L
Sbjct: 44 ILVTPKNLPLLQPLLSRHPSIQPLTLPFPDSPGIPPGVENTKDLPPSSTKSAHVSFMNAL 103
Query: 449 AELSYPITQWFNSHPSPPVAILSDMFLGWTHQLAIQLGIKRINFSPSGAFAISLFDCLWR 628
+ L P+ WF + PSPP I+SDMFLGWTH LA LGI RI FSPS AFA+S+ LWR
Sbjct: 104 SGLRSPLLNWFQTTPSPPSVIISDMFLGWTHHLASDLGIPRIVFSPSAAFALSVIYHLWR 163
Query: 629 DLPKRNDPNDENELIXXXXXXXXXXXXWYQLSNLFRTYVKGDPDSEFTRDGLCANMVSWG 808
++P+ P + +E I QLS ++R+YV GDP SE +DG A++ SWG
Sbjct: 164 NMPQL--PENPSESITFPDLPNSPNWIKSQLSPIYRSYVPGDPQSELVKDGFLADIDSWG 221
Query: 809 VVFNSFNELESVYLDHIKNDLGRKRVFAVGPLV-PPIDAPKERGGSSSIKVNDLLGWLDT 985
+ FNSF LES YL+++K +LG RV+AVGPL+ PP ++ RGG+SS+ V L WLDT
Sbjct: 222 IAFNSFAGLESKYLEYLKIELGHDRVWAVGPLLSPPSESVASRGGTSSVSVPHLEAWLDT 281
Query: 986 CSDRSVVYVCFGSQVVLNNRQMEELALGLEKSGTRFVWSVKEATTGHVAGEYGVVPSGFE 1165
C D VVYVCFGS+ VL Q +LA GLEKSG +FVW VK+ G + +P GFE
Sbjct: 282 CPDDKVVYVCFGSEAVLTEDQSNKLASGLEKSGVQFVWRVKDVEGGRPS-----IPEGFE 336
Query: 1166 DRVAGRGFVIKGWAPQVVILNHRAVGSFLTHCGWNSLLEAIIAGVPMLTWPMGADQYINS 1345
DRVAGRG VI+GWAPQV+IL+HRAVG+FLTHCGWNS+LE I+AGVPML WPMGADQ+I++
Sbjct: 337 DRVAGRGVVIRGWAPQVMILSHRAVGAFLTHCGWNSVLEGIVAGVPMLAWPMGADQFIDA 396
Query: 1346 ELLVERIGVAVKVCQGGLTVPNADELALKFSESVGENQLLSERAKELSQAALNAVKDGGS 1525
LLVE + +AV+VC+G +VP+++ +A K SE + E++ + AKELS AA AV +GGS
Sbjct: 397 TLLVEELKMAVRVCEGKESVPDSEVVASKLSELMEEDREERKLAKELSLAAKEAVSEGGS 456
Query: 1526 SSRDLDGLVREL 1561
S +D++ LV +L
Sbjct: 457 SVKDMESLVEQL 468
Score = 52.4 bits (124), Expect(2) = e-138
Identities = 25/43 (58%), Positives = 32/43 (74%), Gaps = 1/43 (2%)
Frame = +1
Query: 67 TMTLTNSTHILVFPYPAQGHMIALLDLTHQLVI-RGLTITILV 192
T+ +T HIL+FP+PAQGH+I +LD TH L + R L ITILV
Sbjct: 4 TVAVTTVPHILIFPFPAQGHLIPILDFTHYLALRRQLQITILV 46
>gb|AFJ53023.1| UDP-glycosyltransferase 1 [Linum usitatissimum]
Length = 475
Score = 463 bits (1191), Expect(2) = e-135
Identities = 243/436 (55%), Positives = 304/436 (69%), Gaps = 5/436 (1%)
Frame = +2
Query: 281 ILVTPKNLPILNPLLSTHPTIQTLVLPFPIHPSIPPGVENTKDLP----NGSFRTMMRVL 448
ILVTPKNLP+L PLLS HP+IQ L LPFP P IPPGVENTKDLP S + M L
Sbjct: 42 ILVTPKNLPLLQPLLSRHPSIQPLTLPFPDTPHIPPGVENTKDLPPSLTKSSHVSFMYAL 101
Query: 449 AELSYPITQWFNSHPSPPVAILSDMFLGWTHQLAIQLGIKRINFSPSGAFAISLFDCLWR 628
A L P+ WF + PSPP I+SDMFLGWTH LA LGI RI FSPS AFA+S+ LWR
Sbjct: 102 AGLRSPLLNWFQTTPSPPSVIISDMFLGWTHHLATDLGIPRIVFSPSAAFALSVIYHLWR 161
Query: 629 DLPKRNDPNDENELIXXXXXXXXXXXXWYQLSNLFRTYVKGDPDSEFTRDGLCANMVSWG 808
++P+ P +E I QLS ++R+YV GDP SEF +DG A++ SWG
Sbjct: 162 NMPQL--PESPDESITFPDLPNSPSWIKSQLSPIYRSYVPGDPLSEFVKDGFLADIDSWG 219
Query: 809 VVFNSFNELESVYLDHIKNDLGRKRVFAVGPLV-PPIDAPKERGGSSSIKVNDLLGWLDT 985
+ FNSF LES YLD++K +LG RV+AVGPL+ PP ++ RGG+SS+ V DL WLDT
Sbjct: 220 IAFNSFAGLESKYLDYLKIELGHDRVWAVGPLLSPPSESVASRGGTSSVSVADLEAWLDT 279
Query: 986 CSDRSVVYVCFGSQVVLNNRQMEELALGLEKSGTRFVWSVKEATTGHVAGEYGVVPSGFE 1165
C + VVYVCFGS+ VL Q ELA GLEKSG +FVW VK+ V GE +P GFE
Sbjct: 280 CQEGKVVYVCFGSEAVLTVDQSNELASGLEKSGVQFVWRVKD-----VEGERPSIPEGFE 334
Query: 1166 DRVAGRGFVIKGWAPQVVILNHRAVGSFLTHCGWNSLLEAIIAGVPMLTWPMGADQYINS 1345
DRVAGRG VI+GWAPQV+IL+HRAVG+FLTHCGWNS+LE I+AGV ML WPMGADQ+ ++
Sbjct: 335 DRVAGRGVVIRGWAPQVMILSHRAVGAFLTHCGWNSVLEGIVAGVAMLAWPMGADQFTDA 394
Query: 1346 ELLVERIGVAVKVCQGGLTVPNADELALKFSESVGENQLLSERAKELSQAALNAVKDGGS 1525
LLVE + +AV+VC+G VP+++ +A + E + E++ + AKELS AA AV +GGS
Sbjct: 395 TLLVEELKMAVRVCEGKEAVPDSEVVASQLRELMEEDREERKVAKELSLAAKEAVGEGGS 454
Query: 1526 SSRDLDGLVRELQLLS 1573
S +D++ LV +L L+
Sbjct: 455 SVKDMESLVEQLVQLN 470
Score = 48.5 bits (114), Expect(2) = e-135
Identities = 24/43 (55%), Positives = 29/43 (67%), Gaps = 1/43 (2%)
Frame = +1
Query: 67 TMTLTNSTHILVFPYPAQGHMIALLDLTHQLVI-RGLTITILV 192
T+ HIL+FPYPAQGH+I +LD H L + R L ITILV
Sbjct: 2 TVAAITLPHILIFPYPAQGHLIPILDFAHYLALRRQLHITILV 44