BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00008110
         (1954 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI31610.3| unnamed protein product [Vitis vinifera]              420   e-115
ref|XP_002277686.2| PREDICTED: folylpolyglutamate synthase, mito...   412   e-112
ref|XP_002311192.1| folylpolyglutamate synthase [Populus trichoc...   397   e-108
ref|XP_002316257.1| folylpolyglutamate synthase [Populus trichoc...   390   e-106
ref|XP_002524236.1| folylpolyglutamate synthase, putative [Ricin...   388   e-105

>emb|CBI31610.3| unnamed protein product [Vitis vinifera]
          Length = 618

 Score =  420 bits (1080), Expect = e-115
 Identities = 223/332 (67%), Positives = 256/332 (77%), Gaps = 17/332 (5%)
 Frame = +2

Query: 686  QGVPAFTVPQPEEAMSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYLNAGLAI 865
            +GVPAFTVPQPEEAM VLE KASQL+VPLQV  P+D SLLNGL+LGL GEHQYLNAGLAI
Sbjct: 294  RGVPAFTVPQPEEAMCVLEAKASQLDVPLQVIPPMDASLLNGLKLGLEGEHQYLNAGLAI 353

Query: 866  ALCHTWLQKTGHLEGMYLDQTSSLPEQFVRGLANTSFEGRAQIVTDLC--MDRLGDLVFY 1039
            ALC TWLQ+T HLE  YL+QTSSLPEQF++GL   S +GRAQIV D C  ++  GDLVFY
Sbjct: 354  ALCSTWLQRTSHLEINYLEQTSSLPEQFIKGLTTASLQGRAQIVPDRCINIESPGDLVFY 413

Query: 1040 LDGAHSPESMDMCGRWFSVATREDVQQHN---------GSKEQL-----QANLRKDSTKV 1177
            LDGAHSPESM++C RWFS+A +ED QQ           GS  +L         RK++T++
Sbjct: 414  LDGAHSPESMEICARWFSLAIKEDDQQPTLNYQPQDDVGSSRELVQKDYHGTSRKNTTQI 473

Query: 1178 LLFNCMPVRDPQLLLPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDSQVDLSW 1357
            LLFNCM VRDP LLLP L+N CA HGVHF++ALFVPN SVY++VGSHALPL D QVDLSW
Sbjct: 474  LLFNCMSVRDPHLLLPHLMNTCASHGVHFKQALFVPNISVYNRVGSHALPLTDLQVDLSW 533

Query: 1358 QLTLQRVWENLVCGDKTIQGGDEKAADPIREEAIEDASNSIRS-ETSSVFSSLPSAIKWL 1534
            QLTLQR+WE+L      I GGD K  D  REE  +D   S+ S E S+VF SLP AIKWL
Sbjct: 534  QLTLQRLWESL------IHGGDAKNID-TREEGKDDTEKSVISCENSTVFPSLPLAIKWL 586

Query: 1535 RDSAQQNRSVRFQVLVTGSLHLVGDVLRLIKK 1630
            RDS +QN+SVRFQVLVTGSLHLVGDVLRL+KK
Sbjct: 587  RDSVRQNQSVRFQVLVTGSLHLVGDVLRLVKK 618



 Score =  239 bits (609), Expect = 3e-60
 Identities = 116/156 (74%), Positives = 130/156 (83%)
 Frame = +1

Query: 70  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAITKMKIIHVAGTKGKGSTCTFTESILRNC 249
           ITKRSR DKSNK   FD+L+DY+K+L+LDE I+KMKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 108 ITKRSRGDKSNKRVLFDVLFDYLKMLDLDEPISKMKIIHVAGTKGKGSTCTFTESILRNC 167

Query: 250 GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLAHFWWCFNKLKETTTEDVPMPTXXXXXX 429
           GFRTGLFTSPHLID+RERFRLDG+EI ++KFLA+FWWC+++LKE T EDVPMPT      
Sbjct: 168 GFRTGLFTSPHLIDVRERFRLDGVEICEEKFLAYFWWCWDRLKEKTDEDVPMPTFFHFIA 227

Query: 430 XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIV 537
                    EQVDV ILEVGLGG  DATNVVQEPIV
Sbjct: 228 LLAFKIFAAEQVDVVILEVGLGGRLDATNVVQEPIV 263


>ref|XP_002277686.2| PREDICTED: folylpolyglutamate synthase, mitochondrial-like [Vitis
            vinifera]
          Length = 521

 Score =  412 bits (1059), Expect = e-112
 Identities = 217/318 (68%), Positives = 249/318 (78%), Gaps = 3/318 (0%)
 Frame = +2

Query: 686  QGVPAFTVPQPEEAMSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYLNAGLAI 865
            +GVPAFTVPQPEEAM VLE KASQL+VPLQV  P+D SLLNGL+LGL GEHQYLNAGLAI
Sbjct: 213  RGVPAFTVPQPEEAMCVLEAKASQLDVPLQVIPPMDASLLNGLKLGLEGEHQYLNAGLAI 272

Query: 866  ALCHTWLQKTGHLEGMYLDQTSSLPEQFVRGLANTSFEGRAQIVTDLC--MDRLGDLVFY 1039
            ALC TWLQ+T HLE  YL+QTSSLPEQF++GL   S +GRAQIV D C  ++  GDLVFY
Sbjct: 273  ALCSTWLQRTSHLEINYLEQTSSLPEQFIKGLTTASLQGRAQIVPDRCINIESPGDLVFY 332

Query: 1040 LDGAHSPESMDMCGRWFSVATREDVQQHNGSKEQLQANLRKDSTKVLLFNCMPVRDPQLL 1219
            LDGAHSPESM++C RWFS+A +ED QQ      Q Q ++      +LLFNCM VRDP LL
Sbjct: 333  LDGAHSPESMEICARWFSLAIKEDDQQPT-LNYQPQDDVGSSRELILLFNCMSVRDPHLL 391

Query: 1220 LPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDSQVDLSWQLTLQRVWENLVCG 1399
            LP L+N CA HGVHF++ALFVPN SVY++VGSHALPL D QVDLSWQLTLQR+WE+L+  
Sbjct: 392  LPHLMNTCASHGVHFKQALFVPNISVYNRVGSHALPLTDLQVDLSWQLTLQRLWESLI-- 449

Query: 1400 DKTIQGGDEKAADPIREEAIEDASNSIRS-ETSSVFSSLPSAIKWLRDSAQQNRSVRFQV 1576
                  GD K  D  REE  +D   S+ S E S+VF SLP AIKWLRDS +QN+SVRFQV
Sbjct: 450  -----HGDAKNID-TREEGKDDTEKSVISCENSTVFPSLPLAIKWLRDSVRQNQSVRFQV 503

Query: 1577 LVTGSLHLVGDVLRLIKK 1630
            LVTGSLHLVGDVLRL+KK
Sbjct: 504  LVTGSLHLVGDVLRLVKK 521



 Score =  239 bits (609), Expect = 3e-60
 Identities = 116/156 (74%), Positives = 130/156 (83%)
 Frame = +1

Query: 70  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAITKMKIIHVAGTKGKGSTCTFTESILRNC 249
           ITKRSR DKSNK   FD+L+DY+K+L+LDE I+KMKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 27  ITKRSRGDKSNKRVLFDVLFDYLKMLDLDEPISKMKIIHVAGTKGKGSTCTFTESILRNC 86

Query: 250 GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLAHFWWCFNKLKETTTEDVPMPTXXXXXX 429
           GFRTGLFTSPHLID+RERFRLDG+EI ++KFLA+FWWC+++LKE T EDVPMPT      
Sbjct: 87  GFRTGLFTSPHLIDVRERFRLDGVEICEEKFLAYFWWCWDRLKEKTDEDVPMPTFFHFIA 146

Query: 430 XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIV 537
                    EQVDV ILEVGLGG  DATNVVQEPIV
Sbjct: 147 LLAFKIFAAEQVDVVILEVGLGGRLDATNVVQEPIV 182


>ref|XP_002311192.1| folylpolyglutamate synthase [Populus trichocarpa]
            gi|222851012|gb|EEE88559.1| folylpolyglutamate synthase
            [Populus trichocarpa]
          Length = 539

 Score =  397 bits (1019), Expect = e-108
 Identities = 212/331 (64%), Positives = 249/331 (75%), Gaps = 17/331 (5%)
 Frame = +2

Query: 689  GVPAFTVPQPEEAMSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYLNAGLAIA 868
            G+PAFTVPQP+EAM+VLE+KAS+LNV LQVA PLD  LLNGL+L L GEHQYLNAGLAIA
Sbjct: 212  GIPAFTVPQPDEAMNVLEEKASELNVHLQVAEPLDAKLLNGLKLRLEGEHQYLNAGLAIA 271

Query: 869  LCHTWLQKTGHLEGMYLDQTSSLPEQFVRGLANTSFEGRAQIVTD--LCMDRLGDLVFYL 1042
            L  TWLQ+TGH E  +++Q SSLPEQF++GL   S +GRAQIVTD  +  +  GDLVFYL
Sbjct: 272  LSFTWLQRTGHHEFTHMEQASSLPEQFIKGLTTASLQGRAQIVTDQYINAESYGDLVFYL 331

Query: 1043 DGAHSPESMDMCGRWFSVATREDVQ--------QHNGS------KEQLQANLRKDSTKVL 1180
            DGAHSPESM+MC RWFS+A +ED Q        Q+N        +E       K S ++L
Sbjct: 332  DGAHSPESMEMCARWFSLAIKEDSQKRTFNYPPQNNSESTIELVQEHHDERYGKSSMQIL 391

Query: 1181 LFNCMPVRDPQLLLPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDSQVDLSWQ 1360
            LFNCM VRDPQLLLP L+ ACA HGV+F+KALFV N SVY+KVGSHALP  DSQ +LSWQ
Sbjct: 392  LFNCMSVRDPQLLLPPLMKACASHGVYFKKALFVANTSVYYKVGSHALPPTDSQFNLSWQ 451

Query: 1361 LTLQRVWENLVCGDKTIQGGDEKAADPIREEAIEDASNSIRS-ETSSVFSSLPSAIKWLR 1537
              LQRVWENL+  DK   GG+ K A  + EE  EDA  S R+ E S+VF SLP AIKWL+
Sbjct: 452  FALQRVWENLIQSDK---GGEVKHAVAVCEEGKEDAKMSGRTCENSAVFPSLPLAIKWLK 508

Query: 1538 DSAQQNRSVRFQVLVTGSLHLVGDVLRLIKK 1630
            +S Q+NRSV +QVLVTGSLHLVGDVLRL+KK
Sbjct: 509  ESVQRNRSVHYQVLVTGSLHLVGDVLRLVKK 539



 Score =  240 bits (612), Expect = 1e-60
 Identities = 117/156 (75%), Positives = 133/156 (85%)
 Frame = +1

Query: 70  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAITKMKIIHVAGTKGKGSTCTFTESILRNC 249
           ITKRSRADKSN GDRFD+L+DY+K   L+EAI++MKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 28  ITKRSRADKSNNGDRFDVLFDYLK---LEEAISEMKIIHVAGTKGKGSTCTFTESILRNC 84

Query: 250 GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLAHFWWCFNKLKETTTEDVPMPTXXXXXX 429
           GFRTGLFTSPHLID+RERFRLDG +I ++KFLA+FWWC+++LKE +TEDVPMPT      
Sbjct: 85  GFRTGLFTSPHLIDVRERFRLDGKDICEEKFLAYFWWCYDRLKEKSTEDVPMPTYFRFLA 144

Query: 430 XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIV 537
                    EQVDVAILEVGLGG FDATNVVQ+PIV
Sbjct: 145 LLAFKIFSAEQVDVAILEVGLGGKFDATNVVQKPIV 180


>ref|XP_002316257.1| folylpolyglutamate synthase [Populus trichocarpa]
            gi|222865297|gb|EEF02428.1| folylpolyglutamate synthase
            [Populus trichocarpa]
          Length = 587

 Score =  390 bits (1001), Expect = e-106
 Identities = 211/330 (63%), Positives = 249/330 (75%), Gaps = 17/330 (5%)
 Frame = +2

Query: 689  GVPAFTVPQPEEAMSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYLNAGLAIA 868
            G+PAFTVPQP+EAM+VL  KAS+LNV L+VA PLD  LLNGL+L L GEHQYLNAGLAIA
Sbjct: 255  GIPAFTVPQPDEAMNVLVDKASKLNVTLKVAEPLDAKLLNGLKLSLEGEHQYLNAGLAIA 314

Query: 869  LCHTWLQKTGHLEGMYLDQTSSLPEQFVRGLANTSFEGRAQIVTD--LCMDRLGDLVFYL 1042
            L  TWLQ+TGH E    +Q S LPEQF++GL   S +GRAQ V D  +  +R GDLVFYL
Sbjct: 315  LSSTWLQRTGHHEFTCPEQASPLPEQFIKGLTTASLQGRAQKVPDQYINAERYGDLVFYL 374

Query: 1043 DGAHSPESMDMCGRWFSVATREDVQQ---------HNGSKEQL-QANLR----KDSTKVL 1180
            DGAHSPESM+MC RWFS+A +ED Q+         ++GS  +L Q N +    K S ++L
Sbjct: 375  DGAHSPESMEMCARWFSLAVKEDSQKKAFNHPPQNNSGSTIELTQGNPKERDGKISMQIL 434

Query: 1181 LFNCMPVRDPQLLLPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDSQVDLSWQ 1360
            +FNCM VRDPQLLLPRL+ AC +HGV+F+KALFVPN SVY+KVGSHALP  DSQVDLSWQ
Sbjct: 435  VFNCMSVRDPQLLLPRLMKACGNHGVYFKKALFVPNTSVYYKVGSHALP-TDSQVDLSWQ 493

Query: 1361 LTLQRVWENLVCGDKTIQGGDEKAADPIREEAIEDAS-NSIRSETSSVFSSLPSAIKWLR 1537
            L LQRVWENL+  DK   GG+ K AD + EE  +D        E S+VF SLP AIKWLR
Sbjct: 494  LALQRVWENLIQSDK---GGEAKHADAVCEEGKDDTKMGGSTCENSTVFPSLPLAIKWLR 550

Query: 1538 DSAQQNRSVRFQVLVTGSLHLVGDVLRLIK 1627
            +S QQN+SVR+QVLVTGSLHLVGDVLRL+K
Sbjct: 551  ESVQQNQSVRYQVLVTGSLHLVGDVLRLVK 580



 Score =  253 bits (646), Expect = 1e-64
 Identities = 120/156 (76%), Positives = 136/156 (87%)
 Frame = +1

Query: 70  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAITKMKIIHVAGTKGKGSTCTFTESILRNC 249
           ITKRSRADKSNKGDRFDLL+DY+K+LEL+EAI++MKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 28  ITKRSRADKSNKGDRFDLLFDYLKILELEEAISEMKIIHVAGTKGKGSTCTFTESILRNC 87

Query: 250 GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLAHFWWCFNKLKETTTEDVPMPTXXXXXX 429
           GF TGLFTSPHLID+RERFRLDG++I ++KFLA+FWWCF++LKE  TED+PMPT      
Sbjct: 88  GFHTGLFTSPHLIDVRERFRLDGMDICEEKFLAYFWWCFDRLKEKATEDIPMPTYFRFLA 147

Query: 430 XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIV 537
                    EQVDVAILEVGLGG FDATNVVQ+PIV
Sbjct: 148 LLAFKIFAAEQVDVAILEVGLGGKFDATNVVQKPIV 183


>ref|XP_002524236.1| folylpolyglutamate synthase, putative [Ricinus communis]
            gi|223536513|gb|EEF38160.1| folylpolyglutamate synthase,
            putative [Ricinus communis]
          Length = 531

 Score =  388 bits (997), Expect = e-105
 Identities = 206/323 (63%), Positives = 244/323 (75%), Gaps = 10/323 (3%)
 Frame = +2

Query: 692  VPAFTVPQPEEAMSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYLNAGLAIAL 871
            +PAFTVPQ +EAM VLE KAS+L+VPLQVA PLD SLLNGL+LGL GEHQYLNAGLAIAL
Sbjct: 215  IPAFTVPQLDEAMHVLEDKASKLDVPLQVAPPLDPSLLNGLKLGLEGEHQYLNAGLAIAL 274

Query: 872  CHTWLQKTGHLEGMYLDQTSSLPEQFVRGLANTSFEGRAQIVTDLCM--DRLGDLVFYLD 1045
               WLQ+TGH+E  YL+Q+S LPE F+RGL     +GRAQIV D C+  +  GDLVFYLD
Sbjct: 275  SSKWLQRTGHVEFSYLEQSSPLPEHFIRGLTTACLQGRAQIVPDRCVNTETNGDLVFYLD 334

Query: 1046 GAHSPESMDMCGRWFSVATREDVQQ-------HNGSKEQLQANLRKDSTKVLLFNCMPVR 1204
            GAHSPESM+ C RWFS+A ++D QQ       H   +  L  +L   S ++LLFNCM VR
Sbjct: 335  GAHSPESMEACARWFSLAIKDDNQQHTLNSPPHRNYESTLGFHL---SMQILLFNCMSVR 391

Query: 1205 DPQLLLPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDSQVDLSWQLTLQRVWE 1384
            DPQLLLPRL+  CA  GV+F+KALFVPN SVY+KVGS ALP  D QVDLSWQ TLQR+WE
Sbjct: 392  DPQLLLPRLMKTCASQGVYFKKALFVPNTSVYYKVGSQALPPVDPQVDLSWQFTLQRLWE 451

Query: 1385 NLVCGDKTIQGGDEKAADPIREEAIEDASNSIRS-ETSSVFSSLPSAIKWLRDSAQQNRS 1561
            +LV GDK   GG+    D + EE  ++   ++++ E S+VF SLP AIKWLRDS  QNRS
Sbjct: 452  SLVRGDK---GGEANNVDALCEEVKDETGATVKNCENSAVFPSLPLAIKWLRDSVLQNRS 508

Query: 1562 VRFQVLVTGSLHLVGDVLRLIKK 1630
            VR QVLVTGSLHLVGDVLR++KK
Sbjct: 509  VRIQVLVTGSLHLVGDVLRIVKK 531



 Score =  247 bits (630), Expect = 9e-63
 Identities = 117/156 (75%), Positives = 135/156 (86%)
 Frame = +1

Query: 70  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAITKMKIIHVAGTKGKGSTCTFTESILRNC 249
           ITKRSRADKSNKGDRFD L+DY+K+L+L++AI++MKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 27  ITKRSRADKSNKGDRFDFLFDYLKMLDLEQAISEMKIIHVAGTKGKGSTCTFTESILRNC 86

Query: 250 GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLAHFWWCFNKLKETTTEDVPMPTXXXXXX 429
           GFRTGLFTSPHLID+RERFRLDG +I ++KFLA+FWWC+++LKE TTE++PMPT      
Sbjct: 87  GFRTGLFTSPHLIDVRERFRLDGEDICEEKFLAYFWWCYDRLKEKTTEEIPMPTYFRFLA 146

Query: 430 XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIV 537
                    EQVDVAILEVGLGG FDATNVVQ PIV
Sbjct: 147 LLAFKIFAAEQVDVAILEVGLGGKFDATNVVQTPIV 182