BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00007858
         (1864 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]             987   0.0  
gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp....   749   0.0  
gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]         687   0.0  
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]          611   e-172
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]          610   e-172

>dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]
          Length = 533

 Score =  987 bits (2551), Expect = 0.0
 Identities = 487/533 (91%), Positives = 489/533 (91%)
 Frame = +3

Query: 126  MRATHTIISTLMLLFLCNLRCSQSEDDLPSCLILHGVSNYTTPFISNSDYDRLLYVSMQN 305
            MRATHTIISTLMLLFLCNL CSQSEDDLPSCLILHGVSNYTTPFISNSDYDRLLYVSMQN
Sbjct: 1    MRATHTIISTLMLLFLCNLTCSQSEDDLPSCLILHGVSNYTTPFISNSDYDRLLYVSMQN 60

Query: 306  QIFTRSTFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVII 485
            QIFTRSTFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVII
Sbjct: 61   QIFTRSTFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVII 120

Query: 486  DMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSNVFGFSAGYCPTVXXXXXXXXXXX 665
            DMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSS+V GFSAGYCPTV           
Sbjct: 121  DMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSDVLGFSAGYCPTVGSGGHISGGGF 180

Query: 666  XXXXRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXXYAWKLQLLP 845
                RKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIR          YAWKLQLLP
Sbjct: 181  GMMSRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRGGGGGVWGVVYAWKLQLLP 240

Query: 846  VPKHVTVFKLTKHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDGFWLMFLGLYL 1025
            VPKHVTVFKLTKHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDGFWL FLGLYL
Sbjct: 241  VPKHVTVFKLTKHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDGFWLTFLGLYL 300

Query: 1026 GPKEFAVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTK 1205
            GPKE AVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTK
Sbjct: 301  GPKEVAVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTK 360

Query: 1206 VDFAKVPIPLEGINGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYI 1385
            VDFA+VPIPLEGINGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYI
Sbjct: 361  VDFAEVPIPLEGINGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYI 420

Query: 1386 VAWDMDEDLNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXXXXXX 1565
            VAWDMDED NSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTN        
Sbjct: 421  VAWDMDEDFNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNSSISASKA 480

Query: 1566 XXXXRTWGEKYFLSNYDRLVEAKTLIDPNNVFSHPQSIPPLHRGVGCRGNSVI 1724
                RTWGEKYFLSNYDRLV AKTLIDPNNVFSHPQSIPPLHRGVGCRGN VI
Sbjct: 481  IEIARTWGEKYFLSNYDRLVGAKTLIDPNNVFSHPQSIPPLHRGVGCRGNIVI 533


>gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp. glaucum]
          Length = 535

 Score =  749 bits (1935), Expect = 0.0
 Identities = 371/514 (72%), Positives = 412/514 (80%), Gaps = 3/514 (0%)
 Frame = +3

Query: 156  LMLLFLCNLRCSQSEDDLPSCLILHGVSNYTT-PFISNSD-YDRLLYVSMQNQIFTRSTF 329
            ++L+FL N  C+  +DDLPSCL +HGV NYTT    SNSD Y RLLYVSMQNQIFTRSTF
Sbjct: 6    ILLVFLNNFTCAIIDDDLPSCLTIHGVHNYTTHQSTSNSDAYHRLLYVSMQNQIFTRSTF 65

Query: 330  PRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNLNGV 509
            P+P VIILPES DQL N ISCC RGSWTIRLRSGGHSYEGLSH AD+PFVIID+MNLNG+
Sbjct: 66   PQPRVIILPESMDQLANVISCCTRGSWTIRLRSGGHSYEGLSHIADNPFVIIDLMNLNGI 125

Query: 510  SIDLDTQTAWAESGATLGEIYHAIGVSSNVFGFSAGYCPTVXXXXXXXXXXXXXXXRKYG 689
            SIDLDTQTAW ESGATLGEIYHAIG SS    FSAGYCPT                RKYG
Sbjct: 126  SIDLDTQTAWVESGATLGEIYHAIGKSSGTMAFSAGYCPTGGSGGHIAPGGFGMMSRKYG 185

Query: 690  LAADNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXXYAWKLQLLPVPKHVTVF 869
            LAADNVVDA+L+ ANG + DR+SMGEDVFWAIR          YAWKLQL+PVPK+VT+F
Sbjct: 186  LAADNVVDALLVDANGVVLDRESMGEDVFWAIRGGGGGVWGAVYAWKLQLVPVPKNVTIF 245

Query: 870  KLTKHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDG-FWLMFLGLYLGPKEFAV 1046
            +L KH SE+++ASKLL+KWQLVAP L+DDFSLAVL G  KD   WL FLGLYLGPKE A 
Sbjct: 246  RLMKH-SEVEDASKLLHKWQLVAPKLEDDFSLAVLAGTNKDSSIWLTFLGLYLGPKELAS 304

Query: 1047 SSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTKVDFAKVP 1226
            SSMH+KFPELNLL E+C E+SWVEA A+LAGLK V EL +RFL+YDDRAFKTKVDF K  
Sbjct: 305  SSMHKKFPELNLLLEDCMEMSWVEATAELAGLKSVSELKDRFLRYDDRAFKTKVDFPKEA 364

Query: 1227 IPLEGINGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYIVAWDMDE 1406
            IPLEGI GAL+ILKKEQRGFMVMNGQGGMM RIS D+ PFPHRSG LSM+EYIVAWD  E
Sbjct: 365  IPLEGIQGALEILKKEQRGFMVMNGQGGMMDRISTDASPFPHRSGTLSMVEYIVAWDKHE 424

Query: 1407 DLNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXXXXXXXXXXRTW 1586
            DL+S+E+I+WLHQ +DYMG+FV NNPRVGYVNHVD D G IDW N            RTW
Sbjct: 425  DLHSNEFIHWLHQLFDYMGKFVSNNPRVGYVNHVDLDLGRIDWVNKTISSGRAIEIARTW 484

Query: 1587 GEKYFLSNYDRLVEAKTLIDPNNVFSHPQSIPPL 1688
            GEKYF+SNYDRLV AKT+IDP NVF+HPQSIPPL
Sbjct: 485  GEKYFMSNYDRLVRAKTMIDPKNVFNHPQSIPPL 518


>gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]
          Length = 536

 Score =  687 bits (1772), Expect = 0.0
 Identities = 331/510 (64%), Positives = 393/510 (77%), Gaps = 1/510 (0%)
 Frame = +3

Query: 162  LLFLCNLRCSQSEDDLPSCLILHGVSNYTT-PFISNSDYDRLLYVSMQNQIFTRSTFPRP 338
            L+ +C + C+  + +L SCL  +GVSN+T     S+SDY RLLYVSMQNQIFTR  +PRP
Sbjct: 8    LVTICTITCTLGDVNLSSCLTSNGVSNFTALSTSSDSDYHRLLYVSMQNQIFTRPKYPRP 67

Query: 339  SVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNLNGVSID 518
            S+IILP+SK++L  ++ C  RG WTIRLRSGGHSYEGLS+ AD+PFV+ID+MNLN +SID
Sbjct: 68   SMIILPQSKEELAASVVCSNRGLWTIRLRSGGHSYEGLSYVADTPFVVIDLMNLNRISID 127

Query: 519  LDTQTAWAESGATLGEIYHAIGVSSNVFGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAA 698
            L+++TAW ESGATLGEIY AI  +S+  GFS GYCPTV               RKYGLAA
Sbjct: 128  LESKTAWVESGATLGEIYCAISEASDTLGFSGGYCPTVGSGGHISGGGFGMMSRKYGLAA 187

Query: 699  DNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXXYAWKLQLLPVPKHVTVFKLT 878
            DNV+DA+++ ANGA+ DR SMGEDVFWAIR          YAWKLQLLPVPK VTVFKL 
Sbjct: 188  DNVIDALIVDANGAVLDRSSMGEDVFWAIRGGGGGVWGAIYAWKLQLLPVPKQVTVFKLM 247

Query: 879  KHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDGFWLMFLGLYLGPKEFAVSSMH 1058
            K+   I+EASK+L+KWQ+VAP L+DDF+L+VL GA+ +G W  FLGLYLGPKE A+SS+ 
Sbjct: 248  KNFDNIEEASKMLHKWQVVAPALEDDFTLSVLAGADTNGIWFSFLGLYLGPKELAISSVD 307

Query: 1059 QKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTKVDFAKVPIPLE 1238
            Q FPELNL+ E+CKE+SWVE+FA LAGL  V+E+NNRFLKYDDRAFKTKVDF K PIPLE
Sbjct: 308  QNFPELNLVMEDCKEMSWVESFAHLAGLNSVEEMNNRFLKYDDRAFKTKVDFVKEPIPLE 367

Query: 1239 GINGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYIVAWDMDEDLNS 1418
            GI GAL +L KE RGFM  NGQGG+M RIS DS PFPHR G L M+EYIVAWD DED  S
Sbjct: 368  GIKGALTMLTKELRGFMAFNGQGGLMSRISSDSTPFPHRKGTLMMMEYIVAWDRDEDAKS 427

Query: 1419 HEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXXXXXXXXXXRTWGEKY 1598
            +E+I WLH FY+YMGQF+ ++PR+ YVNHVD D G +DWTN            RTWGEKY
Sbjct: 428  YEFIGWLHGFYNYMGQFLPSDPRIAYVNHVDLDLGRLDWTN-STIASNAIEIARTWGEKY 486

Query: 1599 FLSNYDRLVEAKTLIDPNNVFSHPQSIPPL 1688
            FLSNY+RLV AKTLIDP NVF HPQSIPP+
Sbjct: 487  FLSNYERLVRAKTLIDPKNVFHHPQSIPPM 516


>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
          Length = 536

 Score =  611 bits (1576), Expect = e-172
 Identities = 298/511 (58%), Positives = 377/511 (73%), Gaps = 1/511 (0%)
 Frame = +3

Query: 159  MLLFLCNLRCSQSEDDLPSCLILHGVSNYTTPFI-SNSDYDRLLYVSMQNQIFTRSTFPR 335
            + +F   L C+ S DD+ SCL  +GV NYTTP   SNSDY RL ++S+QN +F +ST  +
Sbjct: 19   LFIFFSVLTCALS-DDILSCLTSNGVHNYTTPSSDSNSDYLRLFHLSIQNPLFKKSTISK 77

Query: 336  PSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNLNGVSI 515
            PS+I+LP +K++L NT+ CC RGSWTIRLRSGGHSYEGLS+TAD+PFV+ID+MNLN +SI
Sbjct: 78   PSLIVLPGNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDLMNLNRISI 137

Query: 516  DLDTQTAWAESGATLGEIYHAIGVSSNVFGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLA 695
            D+D++TAW ESGAT+GE+Y+AI  S++ FGF+AG+CPTV               RKYGLA
Sbjct: 138  DMDSETAWVESGATVGELYYAISESTDSFGFTAGWCPTVGTGGHISGGGFGMMSRKYGLA 197

Query: 696  ADNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXXYAWKLQLLPVPKHVTVFKL 875
            ADNV D ILI +NGA+ DRK MGEDVFWA+R          YAWK++LLPVPK VTVF++
Sbjct: 198  ADNVEDVILIDSNGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWKIKLLPVPKKVTVFRV 257

Query: 876  TKHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDGFWLMFLGLYLGPKEFAVSSM 1055
             K+ + I+EAS L++KWQ VA  LDDDF++ +L GA  +G WL+FLGL+LG K  A S M
Sbjct: 258  MKNVN-IEEASFLIHKWQYVADELDDDFTVTILGGANGNGAWLVFLGLHLGCKTVAKSIM 316

Query: 1056 HQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRAFKTKVDFAKVPIPL 1235
             + FPEL L+ EE  E++W E+FA L+GLK V ELNNRFLK DD+AFKTKVDF K  +PL
Sbjct: 317  DKMFPELGLIEEEFLEMNWGESFAYLSGLKTVKELNNRFLKLDDKAFKTKVDFTKETLPL 376

Query: 1236 EGINGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSMIEYIVAWDMDEDLN 1415
            + ING L+IL KE RGF+++N  GG M +IS D  PFPHR+G   M+EYIV+W  DE+  
Sbjct: 377  KVINGVLEILSKEPRGFILLNSLGGKMSKISNDFTPFPHRNGTKLMVEYIVSWSKDEESK 436

Query: 1416 SHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXXXXXXXXXXRTWGEK 1595
            S E+ +WL   YDYM +FV  NPRVGYVN++D D G IDW+N            R WGEK
Sbjct: 437  SDEFFDWLRNIYDYMEEFVSKNPRVGYVNNIDLDLGGIDWSN-KNSSNNAIEIARNWGEK 495

Query: 1596 YFLSNYDRLVEAKTLIDPNNVFSHPQSIPPL 1688
            YFLSNY+RL+ AKTLIDPNN+F+HPQSIPP+
Sbjct: 496  YFLSNYERLIRAKTLIDPNNIFNHPQSIPPM 526


>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
          Length = 554

 Score =  610 bits (1574), Expect = e-172
 Identities = 302/525 (57%), Positives = 381/525 (72%), Gaps = 5/525 (0%)
 Frame = +3

Query: 129  RATHTIISTLMLL----FLCNLRCSQSEDDLPSCLILHGVSNYTTPFI-SNSDYDRLLYV 293
            + T  + S+L ++    F   L C+ S DD+ SCL  +GV NYTTP   SNSDY RL ++
Sbjct: 10   KITKNLYSSLFIIIFFFFFSVLTCALS-DDILSCLTSNGVHNYTTPSSDSNSDYLRLYHL 68

Query: 294  SMQNQIFTRSTFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSP 473
            S+QN +F +ST  +PS+I+LP +K++L NT+ CC RGSWTIRLRSGGHSYEGLS+TAD+P
Sbjct: 69   SIQNPLFEKSTISKPSLIVLPRNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTP 128

Query: 474  FVIIDMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSNVFGFSAGYCPTVXXXXXXX 653
            FV+ID+MNLN +SID+D++TAW ESGATLGE+Y+AI   ++  GF+AG+CPTV       
Sbjct: 129  FVLIDLMNLNRISIDIDSETAWVESGATLGELYYAITELTDSLGFTAGWCPTVGSGGHIS 188

Query: 654  XXXXXXXXRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRXXXXXXXXXXYAWKL 833
                    RKYGLAADNV D ILI + GA+ DRK MGEDVFWA+R          YAWK+
Sbjct: 189  GGGFGMMSRKYGLAADNVEDVILIDSKGAILDRKLMGEDVFWAVRGGGGGVWGAIYAWKI 248

Query: 834  QLLPVPKHVTVFKLTKHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDGFWLMFL 1013
            +LLPVPK VTVF++TK+ + I+EAS L++KWQ VA  LDDDF++++L GA  +  W++FL
Sbjct: 249  KLLPVPKKVTVFRVTKNVN-IEEASFLIHKWQYVADELDDDFTVSILGGANGNEVWVIFL 307

Query: 1014 GLYLGPKEFAVSSMHQKFPELNLLSEECKEVSWVEAFAQLAGLKEVDELNNRFLKYDDRA 1193
            GL+LG K  A S + +KFPEL L+ EE  E++W E+FA L+GLK V ELNNRFLK+DDRA
Sbjct: 308  GLHLGCKTVAKSIIDKKFPELGLIEEEFLEMNWGESFAYLSGLKTVKELNNRFLKFDDRA 367

Query: 1194 FKTKVDFAKVPIPLEGINGALQILKKEQRGFMVMNGQGGMMGRISRDSIPFPHRSGMLSM 1373
            FKTKVDF K  +PLE I+G L+IL KE RGF+ +NG GG M +IS D  PFPHR G   M
Sbjct: 368  FKTKVDFTKETLPLEAIDGLLEILSKEPRGFIALNGFGGKMSKISNDFTPFPHRKGTKLM 427

Query: 1374 IEYIVAWDMDEDLNSHEYINWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNXXXX 1553
            +EYIVAW  DE+  S E+ +WL   YDYM  FV  NPRVGYVNH+D D G IDW++    
Sbjct: 428  VEYIVAWSKDEESKSDEFFDWLRNIYDYMEMFVSKNPRVGYVNHIDLDLGGIDWSD-KNS 486

Query: 1554 XXXXXXXXRTWGEKYFLSNYDRLVEAKTLIDPNNVFSHPQSIPPL 1688
                    R WGEKYFLSNY+RL+ AKTLIDPNNVF+HPQSIPP+
Sbjct: 487  SNNAIEIARNWGEKYFLSNYERLIRAKTLIDPNNVFNHPQSIPPM 531