BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00007724
         (1680 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q42965.1|NRL4A_TOBAC RecName: Full=Bifunctional nitrilase/nit...   602   e-170
ref|XP_002272837.1| PREDICTED: bifunctional nitrilase/nitrile hy...   602   e-170
sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nit...   601   e-169
ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hy...   601   e-169
gb|AFK41272.1| unknown [Lotus japonicus]                              599   e-169

>sp|Q42965.1|NRL4A_TOBAC RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4A;
            Short=TNIT4A; AltName: Full=Cyanoalanine nitrilase A;
            AltName: Full=Nitrilase 4A gi|1171482|dbj|BAA09645.1|
            nitrilase [Nicotiana tabacum]
          Length = 349

 Score =  602 bits (1553), Expect = e-170
 Identities = 284/353 (80%), Positives = 322/353 (91%)
 Frame = -2

Query: 1586 MALVPTPLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLISD 1407
            MALVPTP ++      P+ AEVDMG +S+  TVRATVVQASTIFYDTPATL KAERL+++
Sbjct: 1    MALVPTPAVNEG----PLFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAE 56

Query: 1406 AALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHLGA 1227
            AA YG+QLVVFPEAFIGGYPRGS FG+++G+RT KGKEEFRKYHASAIDVPGPEV+ L A
Sbjct: 57   AASYGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAA 116

Query: 1226 MAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGSTIP 1047
            MAGKYKVYLVMGVIER+GYTLYCT+LFFDSQGH+LGKHRK+MPT+LER+IWGFGDGSTIP
Sbjct: 117  MAGKYKVYLVMGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIP 176

Query: 1046 VFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 867
            V++TP+GKIG  ICWEN+MPLLRTAMY KGIEIYCAPTAD+RD+WQASMTHIALEGGCFV
Sbjct: 177  VYDTPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFV 236

Query: 866  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEALIS 687
            LSANQFCRRKDYPPPPEY FSGT+EDL+P+S+VCAGGSVIISPSG +LAGPNY GEALIS
Sbjct: 237  LSANQFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALIS 296

Query: 686  ADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKTEGSHK 528
            ADLD GEI RAKFDFDVVGHY+RP+VL+LIVRDH  +PV+FTS ++K E S K
Sbjct: 297  ADLDLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 349


>ref|XP_002272837.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4B-like [Vitis
            vinifera]
          Length = 347

 Score =  602 bits (1552), Expect = e-170
 Identities = 287/353 (81%), Positives = 323/353 (91%)
 Frame = -2

Query: 1586 MALVPTPLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLISD 1407
            MAL+P  + D      P+ AEVDMG+DS+A TVRATVVQAST+FYDTPATL KAERL+++
Sbjct: 1    MALIPATVNDR-----PLFAEVDMGADSSAPTVRATVVQASTVFYDTPATLDKAERLLAE 55

Query: 1406 AALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHLGA 1227
            A+ YGSQLVVFPEAFIGGYPRGSNFG+T+G+RT KG+E+FRKYHA+AIDVPGPEV+ L A
Sbjct: 56   ASSYGSQLVVFPEAFIGGYPRGSNFGVTIGNRTAKGREDFRKYHAAAIDVPGPEVDRLAA 115

Query: 1226 MAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGSTIP 1047
            MAGKYKVYLVMGVIER+GYTLYCT+LFFDSQGHYLGKHRK+MPT+LER+IWGFGDGSTIP
Sbjct: 116  MAGKYKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKVMPTALERIIWGFGDGSTIP 175

Query: 1046 VFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 867
            V+ETPIGKIG  ICWEN+MPLLRTAMY KGIEIYCAPTADARD+WQ+SMTHIALEGGCFV
Sbjct: 176  VYETPIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADARDIWQSSMTHIALEGGCFV 235

Query: 866  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEALIS 687
            LSANQFCRRKDYPPPPEY FSG D DL+P+SVVCAGGSVIISPSGT+LAGPNY+GEALIS
Sbjct: 236  LSANQFCRRKDYPPPPEYEFSGAD-DLTPDSVVCAGGSVIISPSGTVLAGPNYDGEALIS 294

Query: 686  ADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKTEGSHK 528
            ADLD GEI RAKFDFDVVGHYSRP+VL+L+V+D+PT PV FTSA+ KTE   K
Sbjct: 295  ADLDLGEIARAKFDFDVVGHYSRPEVLSLVVKDNPTKPVTFTSASVKTEDFQK 347


>sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4B;
            Short=TNIT4B; AltName: Full=Cyanoalanine nitrilase B;
            AltName: Full=Nitrilase 4B gi|1181615|dbj|BAA11770.1|
            nitrilase [Nicotiana tabacum]
          Length = 348

 Score =  601 bits (1550), Expect = e-169
 Identities = 286/353 (81%), Positives = 323/353 (91%)
 Frame = -2

Query: 1586 MALVPTPLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLISD 1407
            MALVPTP+++      P+ AEVDMG +S+  TVRATVVQASTIFYDTPATL KAERL+++
Sbjct: 1    MALVPTPVVNEG----PMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAE 56

Query: 1406 AALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHLGA 1227
            AA YG+QLVVFPEAFIGGYPRGS FG+++G+RT KGKEEFRKYHASAIDVPGPEV+ L A
Sbjct: 57   AASYGAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAA 116

Query: 1226 MAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGSTIP 1047
            MAGKYKVYLVMGVIER+GYTLYCT+LFFDSQGHYLGKHRK+MPT+LER+IWGFGDGSTIP
Sbjct: 117  MAGKYKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIP 176

Query: 1046 VFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 867
            V++TP+GKIG  ICWEN+MPLLRTAMY KGIEIYCAPTAD+RD+WQASMTHIALEGGCFV
Sbjct: 177  VYDTPLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFV 236

Query: 866  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEALIS 687
            LSANQFCRRKDYPPPPEY FSGT EDL+P+S+VCAGGSVIISPSG +LAGPNYEGEALIS
Sbjct: 237  LSANQFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALIS 295

Query: 686  ADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKTEGSHK 528
            ADLD GEI RAKFDFDVVGHY+RP+VL+LIVRDH  +PV+FTS ++K E S K
Sbjct: 296  ADLDLGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348


>ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A-like
            [Glycine max]
          Length = 350

 Score =  601 bits (1549), Expect = e-169
 Identities = 287/353 (81%), Positives = 319/353 (90%)
 Frame = -2

Query: 1586 MALVPTPLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLISD 1407
            MALV T    N G   P+ AEVDMGSD  A TVRATVVQASTIFYDTPATL KAERL+++
Sbjct: 1    MALVTTNPTINEG---PLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAE 57

Query: 1406 AALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHLGA 1227
            AA YGSQLVVFPEAF+GGYPRGS FGL++G+RT KG+EEFRKYH++AIDVPGPEV+ L A
Sbjct: 58   AASYGSQLVVFPEAFVGGYPRGSAFGLSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLAA 117

Query: 1226 MAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGSTIP 1047
            MAGKYKV+LVMGVIER+GYTLYCT+LFFDSQGHYLGKHRK+MPT+LERVIWGFGDGSTIP
Sbjct: 118  MAGKYKVHLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERVIWGFGDGSTIP 177

Query: 1046 VFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGCFV 867
            VFETP+GKIG  ICWEN+MPLLRTAMY KG+EIYCAPTAD+RD+WQASMTHIALEGGCFV
Sbjct: 178  VFETPVGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADSRDVWQASMTHIALEGGCFV 237

Query: 866  LSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEALIS 687
            LSANQFCRRKDYPPPPEY F+GT+EDL+P+SVVCAGGSVIISPSG +LAGPNY+GEALIS
Sbjct: 238  LSANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPSGAVLAGPNYDGEALIS 297

Query: 686  ADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKTEGSHK 528
            ADLD GEI RAKFDFDVVGHYSRP+VL+LIV+DHPT PV FTS + K E   K
Sbjct: 298  ADLDLGEIARAKFDFDVVGHYSRPEVLSLIVKDHPTNPVTFTSTSTKIEDKTK 350


>gb|AFK41272.1| unknown [Lotus japonicus]
          Length = 346

 Score =  599 bits (1544), Expect = e-169
 Identities = 287/350 (82%), Positives = 319/350 (91%), Gaps = 2/350 (0%)
 Frame = -2

Query: 1586 MALVPT--PLIDNNGAPPPVIAEVDMGSDSTALTVRATVVQASTIFYDTPATLAKAERLI 1413
            MALVPT  P I+N     P+IAEVDMGSDS A TVRATVVQASTIFYDTPATL KAERL+
Sbjct: 1    MALVPTTTPTINNG----PLIAEVDMGSDSNAPTVRATVVQASTIFYDTPATLDKAERLL 56

Query: 1412 SDAALYGSQLVVFPEAFIGGYPRGSNFGLTVGSRTLKGKEEFRKYHASAIDVPGPEVEHL 1233
            ++AA YGSQLVVFPEAFIGGYPRGSNFG+ +G RT KG+E+FRKYH+SAIDVPGPEV+ L
Sbjct: 57   AEAAGYGSQLVVFPEAFIGGYPRGSNFGIIIGIRTAKGREDFRKYHSSAIDVPGPEVDRL 116

Query: 1232 GAMAGKYKVYLVMGVIEREGYTLYCTILFFDSQGHYLGKHRKLMPTSLERVIWGFGDGST 1053
             AMAGKYKV+LVMGVIER+GYTLYCT+LFFDSQGHYLGKHRKLMPT +ERV+WGFGDGST
Sbjct: 117  AAMAGKYKVHLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKLMPTGMERVMWGFGDGST 176

Query: 1052 IPVFETPIGKIGGLICWENKMPLLRTAMYGKGIEIYCAPTADARDLWQASMTHIALEGGC 873
            IPVFETP+GKIG +ICWEN+MPLLRTAMY KG+EIYCAPTADAR++WQASMTHIALEGGC
Sbjct: 177  IPVFETPLGKIGAVICWENRMPLLRTAMYAKGVEIYCAPTADAREVWQASMTHIALEGGC 236

Query: 872  FVLSANQFCRRKDYPPPPEYTFSGTDEDLSPESVVCAGGSVIISPSGTILAGPNYEGEAL 693
            FVLSANQFCRRKDYPPPPEY F+GT+EDL+P+SVVCAGGSVIISP G +LAGPNYEGEAL
Sbjct: 237  FVLSANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPLGNVLAGPNYEGEAL 296

Query: 692  ISADLDFGEIVRAKFDFDVVGHYSRPDVLNLIVRDHPTTPVAFTSAAAKT 543
            ISADLD GEI RAKFDFDVVGHYSRP+VL+L V+DHPT PV F S + K+
Sbjct: 297  ISADLDLGEIARAKFDFDVVGHYSRPEVLSLSVKDHPTNPVTFASTSTKS 346