BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00007093
         (1442 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       553   e-155
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   538   e-150
ref|XP_002267485.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   481   e-133
ref|XP_002267599.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   479   e-133
ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]...   471   e-130

>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  553 bits (1426), Expect = e-155
 Identities = 276/430 (64%), Positives = 331/430 (76%)
 Frame = +3

Query: 3    SPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGPNWKNLRRISAVELLSPS 182
            SPSA E+CL+ KNDIIFANRP  L+GK+LGHDYTT++WA YGPNW+NLR+ISA+ELLS +
Sbjct: 77   SPSAAEDCLA-KNDIIFANRPHLLMGKILGHDYTTLLWAPYGPNWRNLRKISAIELLSAN 135

Query: 183  RLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELSSKFFALTYNVIMRMMAGKRYYGEQVGD 362
            RLQ  S +R DEVKS+++RLS QG +++ VE+ +  F LT NV+M M+AGKRYYG QV +
Sbjct: 136  RLQSYSDIRHDEVKSMVQRLSGQGNEYRTVEMKTMIFELTLNVMMTMIAGKRYYGGQVEE 195

Query: 363  SEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEMLGLKAKRDKFXXXXXXX 542
             E+ KRFQE+V+ TL +S  SN+GDFLPI  WIG  KG + ++  LK KRDKF       
Sbjct: 196  LEQAKRFQEIVQGTLAISGASNIGDFLPILSWIG-IKGIEKKVWNLKEKRDKFLQELIEE 254

Query: 543  XXXXXXXXKFDREEKQTPLIQVLLSLQEAEPEHYTDDVVKGIMWDLFAAGTDTSKSTMEW 722
                        +EK   LI VLLSLQE EPE+YTD+++ GI+W L AAGTDTS  TMEW
Sbjct: 255  HRISRSGG-MQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEW 313

Query: 723  AMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLPYVHSIVMETLRMYPPGPLMIPH 902
            AMSLLLNNP V+KKAQ EID ++E GRL++ESDV KLPY+HSI+ ETLR+YP GPL++PH
Sbjct: 314  AMSLLLNNPQVIKKAQAEIDNNLEQGRLINESDVNKLPYLHSIITETLRIYPAGPLLVPH 373

Query: 903  EASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTNFVPERFLGLEGTRVGFKLMPFG 1082
            E+SEECIVGGY VP GTMLLVN+W I+ DPN+W EPT F PERF G EGTR GFKLMPFG
Sbjct: 374  ESSEECIVGGYKVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFG 433

Query: 1083 VGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMVDMSQQKLGATMHKALPLEAKCRP 1262
             GRR CPGEGLA RV+ L L ALI CFDWERVGE+MVDMS+   G T+ K  PLEAKCRP
Sbjct: 434  SGRRGCPGEGLAMRVVALALGALIQCFDWERVGEEMVDMSEGP-GLTLPKVHPLEAKCRP 492

Query: 1263 RKNALNFIDK 1292
            R  ALNFI +
Sbjct: 493  RSTALNFISQ 502


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  538 bits (1386), Expect = e-150
 Identities = 267/433 (61%), Positives = 329/433 (75%), Gaps = 2/433 (0%)
 Frame = +3

Query: 3    SPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGPNWKNLRRISAVELLSPS 182
            SPSA EECL+ KNDI FANRPR +IGK  G++YTT+ WA YG NW+NLRRISA+E+ SP+
Sbjct: 79   SPSAAEECLT-KNDIGFANRPRLMIGKHSGNNYTTLAWASYGQNWRNLRRISALEMFSPN 137

Query: 183  RLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELSSKFFALTYNVIMRMMAGKRYYGEQVGD 362
            R+QM S +R DEVKS+++RL   G ++  VE+ S FF LT N++MRM+AGKRYYGE V D
Sbjct: 138  RIQMFSEIRTDEVKSMVRRLV-AGYEYHKVEMKSVFFELTLNIMMRMIAGKRYYGEHVAD 196

Query: 363  SEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEMLGLKAKRDKFXXXXXXX 542
             E+ ++F+E+V ET  LS  SNVGDFLP+ RWIG  KG +  ++ LK KRD+F       
Sbjct: 197  LEQARKFKEIVEETFELSGASNVGDFLPLLRWIGY-KGIEKRLIRLKNKRDEFLQELIQE 255

Query: 543  XXXXXXXXK-FDREEKQTPLIQVLLSLQEAEPEHYTDDVVKGIMWDLFAAGTDTSKSTME 719
                    K F  EEK+  LI VLLSLQE EPE+YTD++++G++W LFAAGTDTS  TME
Sbjct: 256  RRKLRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTME 315

Query: 720  WAMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLPYVHSIVMETLRMYPPGPLMIP 899
            WAMSLLLNNP ++KKAQ EIDL +EPGR +DESD+ KLPY+H I+ ETLRMYP GPL+IP
Sbjct: 316  WAMSLLLNNPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIP 375

Query: 900  HEASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTNFVPERFLGLE-GTRVGFKLMP 1076
            HE+SE+C +GGYN+P GTMLLVNLW I+NDPNLW EP  F PERF G + G R GFKLMP
Sbjct: 376  HESSEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGVRDGFKLMP 435

Query: 1077 FGVGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMVDMSQQKLGATMHKALPLEAKC 1256
            FG GRR CPGEGLA RV+ LTL +L+ CFDW RVGE+MV+MS +  G T+ K  PLEA C
Sbjct: 436  FGSGRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGEEMVEMS-EGTGLTLPKLHPLEAHC 494

Query: 1257 RPRKNALNFIDKL 1295
            RPR   LNF+ ++
Sbjct: 495  RPRSTMLNFLSQV 507


>ref|XP_002267485.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 507

 Score =  481 bits (1238), Expect = e-133
 Identities = 238/437 (54%), Positives = 321/437 (73%), Gaps = 6/437 (1%)
 Frame = +3

Query: 3    SPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGPNWKNLRRISAVELLSPS 182
            SPSA EECLS KND++FANRP+ L GK +G++YT+M WA+YG +W+NLRRIS +E+LS S
Sbjct: 78   SPSAAEECLS-KNDVVFANRPQLLSGKYIGYNYTSMAWANYGDHWRNLRRISTLEILSTS 136

Query: 183  RLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELSSKFFALTYNVIMRMMAGKRYYGEQVGD 362
            R+QMLS +R DEV+S++ RL + G   + V++ + FF +T NV+MRM+AGKRYYG  V +
Sbjct: 137  RIQMLSGIRSDEVRSLLLRLLENGA--ETVDMKTAFFEMTMNVMMRMIAGKRYYGGNVVE 194

Query: 363  SEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEMLGLKAKRDKFXXXXXXX 542
             EE  +FQE++ +T RL D +N+GD+LP+ RW+G  KG +  +  L+ KRD+F       
Sbjct: 195  VEETAKFQEIIEDTFRLGDTTNIGDYLPVLRWLGV-KGKEKGLRELQRKRDRFMQGLIEE 253

Query: 543  XXXXXXXXKFDRE-----EKQTPLIQVLLSLQEAEPEHYTDDVVKGIMWDLFAAGTDTSK 707
                     +        EK+T +I+VLLSLQE E E+YTD++++G+M  L  AGTDT+ 
Sbjct: 254  HRTRMAKESYSSSSCRVGEKKT-MIEVLLSLQEKEAEYYTDEIIRGLMLALLGAGTDTTS 312

Query: 708  STMEWAMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLPYVHSIVMETLRMYPPGP 887
            +T+EWAMSLLLNNP VLKKAQ E+D  + P  L++ESD+ +LPY+H I+ ET RMYP GP
Sbjct: 313  ATLEWAMSLLLNNPEVLKKAQMEMDNQLGPNHLIEESDLSQLPYLHCIIRETQRMYPAGP 372

Query: 888  LMIPHEASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTNFVPERF-LGLEGTRVGF 1064
            + +PHE+S+EC+VGGY++PRGTMLLVN+W I+NDP +W EP  F+PERF +GLEG   G 
Sbjct: 373  I-VPHESSKECMVGGYHIPRGTMLLVNIWGIQNDPKVWKEPRKFLPERFEVGLEGEGHGL 431

Query: 1065 KLMPFGVGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMVDMSQQKLGATMHKALPL 1244
            +LMPFG GRR CPGEGLA R++ L L +LI CFDWERVGE  VDMS + +G T+ KA PL
Sbjct: 432  RLMPFGSGRRGCPGEGLAIRMVGLVLGSLIQCFDWERVGEGKVDMS-EGIGLTLPKAQPL 490

Query: 1245 EAKCRPRKNALNFIDKL 1295
             AKCRPR   +N + ++
Sbjct: 491  LAKCRPRPALINVLSQI 507


>ref|XP_002267599.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 508

 Score =  479 bits (1234), Expect = e-133
 Identities = 235/437 (53%), Positives = 320/437 (73%), Gaps = 6/437 (1%)
 Frame = +3

Query: 3    SPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGPNWKNLRRISAVELLSPS 182
            SPSA EECLS KND++FANRP+ L GK +G++YT+M WA+YG +W+NLRRISA+E+LS S
Sbjct: 78   SPSAAEECLS-KNDVVFANRPQLLAGKYIGYNYTSMAWANYGDHWRNLRRISALEILSTS 136

Query: 183  RLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELSSKFFALTYNVIMRMMAGKRYYGEQVGD 362
            R+QMLS +R DEV+S++ RL + G   + V++ + FF +T NV+MRM+AGKRYYG  V +
Sbjct: 137  RIQMLSGIRSDEVRSLLLRLLENGT--ETVDMKTTFFEVTMNVMMRMIAGKRYYGGNVVE 194

Query: 363  SEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEMLGLKAKRDKFXXXXXXX 542
             EE  +FQE++ +T RL D +N+GD+LP+ RW+G  KG +  +  L+ KRD+F       
Sbjct: 195  VEETAKFQEIIEDTFRLGDTTNIGDYLPVLRWLGV-KGKEKGLRELQRKRDRFMQGLIEE 253

Query: 543  XXXXXXXXKFDRE-----EKQTPLIQVLLSLQEAEPEHYTDDVVKGIMWDLFAAGTDTSK 707
                     +        EK+  +I+VLLSLQE E E+YTD++++G+M  L  AG DT+ 
Sbjct: 254  HRTRMAKESYSSSSCRAGEKKKTMIEVLLSLQEKEAEYYTDEIIRGLMLALLGAGIDTTS 313

Query: 708  STMEWAMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLPYVHSIVMETLRMYPPGP 887
            +T+EWAMSLLLNNP VLKKAQ E+D  + P  L++ESD+ +LPY+H I+ ET RMYP GP
Sbjct: 314  ATLEWAMSLLLNNPEVLKKAQMEMDNQLGPNHLIEESDLSQLPYLHCIIRETQRMYPAGP 373

Query: 888  LMIPHEASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTNFVPERF-LGLEGTRVGF 1064
            + +PHE+S+EC+VGGY++PRGTMLLVN+W I+NDP +W EP  F+PERF +GLEG   G 
Sbjct: 374  I-VPHESSKECMVGGYHIPRGTMLLVNIWGIQNDPEVWKEPRKFLPERFEVGLEGEGHGL 432

Query: 1065 KLMPFGVGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMVDMSQQKLGATMHKALPL 1244
            +LMPFG GRR CPGEGLA R++ L L +LI CFDW+RVGE  VDMS + +G T+ +A PL
Sbjct: 433  RLMPFGSGRRGCPGEGLAIRMVGLVLGSLIQCFDWKRVGEGKVDMS-EGIGLTLPRAQPL 491

Query: 1245 EAKCRPRKNALNFIDKL 1295
             AKCRPR   +N + ++
Sbjct: 492  LAKCRPRPALINLLSQI 508


>ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]
            gi|223550831|gb|EEF52317.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 500

 Score =  471 bits (1212), Expect = e-130
 Identities = 236/433 (54%), Positives = 310/433 (71%), Gaps = 2/433 (0%)
 Frame = +3

Query: 3    SPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGPNWKNLRRISAVELLSPS 182
            SPSA EECL+ K D +FANRPR L GK +G++YT++ WA YG  W+NLR++SA+E+LS  
Sbjct: 76   SPSAAEECLT-KCDTVFANRPRLLAGKHIGYNYTSLAWAPYGDLWRNLRKVSALEILSSH 134

Query: 183  RLQMLSAMRFDEVKSIIKRL--SDQGCKHQNVELSSKFFALTYNVIMRMMAGKRYYGEQV 356
            RLQ+LS++R DEVK +I+RL  +++ C+ + V++ S FF L  NV+MRM+AGKRYYGE V
Sbjct: 135  RLQLLSSIRRDEVKLLIQRLFRNNKDCR-EKVDMKSAFFELMLNVMMRMIAGKRYYGENV 193

Query: 357  GDSEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEMLGLKAKRDKFXXXXX 536
               EE KRF+E+VRET   +  SN+GDFLP+   +GG    +  +  L  +RD F     
Sbjct: 194  EQVEEAKRFREIVRETFLAAGTSNMGDFLPLVAVVGGQ---EKRLKELGKRRDGFIQDLI 250

Query: 537  XXXXXXXXXXKFDREEKQTPLIQVLLSLQEAEPEHYTDDVVKGIMWDLFAAGTDTSKSTM 716
                          EE+   +I+VLLSLQE+EPE+YTD+ +K +M  L AAGTDTS +TM
Sbjct: 251  DEHRKRMAACS--SEERNKTMIEVLLSLQESEPEYYTDETIKSLMLVLLAAGTDTSAATM 308

Query: 717  EWAMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLPYVHSIVMETLRMYPPGPLMI 896
            EWAMSLL+NNP  LKKAQ EID  +   RL++ESD  KLPY++ I+ E +RMYP GPL++
Sbjct: 309  EWAMSLLVNNPEALKKAQTEIDSVIGHDRLINESDTSKLPYLNCIINEVMRMYPAGPLLV 368

Query: 897  PHEASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTNFVPERFLGLEGTRVGFKLMP 1076
            PHE+SEEC +GGY VP GTMLLVNLW I+NDP +W EP NF PERF G EG R GF+LMP
Sbjct: 369  PHESSEECFIGGYRVPAGTMLLVNLWSIQNDPRVWEEPRNFKPERFEGCEGVRDGFRLMP 428

Query: 1077 FGVGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMVDMSQQKLGATMHKALPLEAKC 1256
            FG GRR CPGEGLA R++ L +  L+ CFDWERVG++M+DM+ + +G TM KA PL  +C
Sbjct: 429  FGSGRRSCPGEGLALRMVGLGIGTLLQCFDWERVGKEMIDMT-EGVGLTMPKAQPLVVQC 487

Query: 1257 RPRKNALNFIDKL 1295
             PR + +N + +L
Sbjct: 488  SPRPSMVNLLSQL 500