BLASTX nr result
ID: Coptis25_contig00006553
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00006553
(3777 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003612653.1| ATP-dependent DNA helicase PIF1 [Medicago tr... 119 3e-46
ref|XP_003606913.1| ATP-dependent DNA helicase PIF1 [Medicago tr... 119 5e-46
ref|XP_003609124.1| ATP-dependent DNA helicase PIF1 [Medicago tr... 119 8e-46
ref|XP_003609074.1| ATP-dependent DNA helicase PIF1 [Medicago tr... 119 8e-46
gb|AAK54302.1|AC034258_20 putative helicase [Oryza sativa Japoni... 115 5e-44
>ref|XP_003612653.1| ATP-dependent DNA helicase PIF1 [Medicago truncatula]
gi|355513988|gb|AES95611.1| ATP-dependent DNA helicase
PIF1 [Medicago truncatula]
Length = 1427
Score = 119 bits (298), Expect(2) = 3e-46
Identities = 65/159 (40%), Positives = 89/159 (55%), Gaps = 9/159 (5%)
Frame = +1
Query: 772 LIGGRLRQQFVVDAYACMEEARLWWYRNNQAMVRAELYKGLVDVVVAGDTTPASVG---- 939
+IGGRL QQ+VVDA+ +EE RL W R NQ ++R +LY+ + D VV GDT A+ G
Sbjct: 309 IIGGRLFQQYVVDAFTAIEEERLRWVRQNQTVLRTDLYRNVCDAVVRGDTIAAATGKRIV 368
Query: 940 ----XXXXXXXXXXXXXXXXXXCR*VGPPSLFITFT*NPNWPEIKEVIEK-SGQKAADRP 1104
CR G P +F+TFT NP WPEI+ +++K GQ DRP
Sbjct: 369 LPSSFTGGSRYMVQNYQDAMAICRTFGNPDIFMTFTANPKWPEIQYMLQKIPGQSVDDRP 428
Query: 1105 DTISRVFMMKLDELMKDLRHGRQFDLVNAGLIASMYQTR 1221
D +RVF MKLD+LMK + G+ F + + + +Q R
Sbjct: 429 DIKTRVFKMKLDQLMKHIVEGQYFGKIISAIYTIEFQKR 467
Score = 95.5 bits (236), Expect(2) = 3e-46
Identities = 52/101 (51%), Positives = 67/101 (66%), Gaps = 8/101 (7%)
Frame = +3
Query: 504 AAIMVGD----GSGRDIIVDRKDSGFQRISQVYRKFMAMQYPILFPYGEDGFTVDIPYSS 671
A ++VGD RDI+++ K G QRI + + KFMAMQYP+LFPY EDGF +DI YS
Sbjct: 215 AGLIVGDFGESNGRRDIVIEHKTDGLQRIKEYHPKFMAMQYPLLFPYAEDGFHLDIYYS- 273
Query: 672 VQNNVHRKK----KTVSKREHYAYRLQERVNEGQTLILDWR 782
QN+ RKK K V+ RE+YAYR+Q+R E TLI+ R
Sbjct: 274 -QNHAKRKKKKKRKKVTMREYYAYRIQQRPCEANTLIIGGR 313
>ref|XP_003606913.1| ATP-dependent DNA helicase PIF1 [Medicago truncatula]
gi|355507968|gb|AES89110.1| ATP-dependent DNA helicase
PIF1 [Medicago truncatula]
Length = 1558
Score = 119 bits (298), Expect(2) = 5e-46
Identities = 65/159 (40%), Positives = 89/159 (55%), Gaps = 9/159 (5%)
Frame = +1
Query: 772 LIGGRLRQQFVVDAYACMEEARLWWYRNNQAMVRAELYKGLVDVVVAGDTTPASVG---- 939
+IGGRL QQ+VVDA+ +EE RL W R NQ ++R +LY+ + D VV GDT A+ G
Sbjct: 309 IIGGRLFQQYVVDAFTAIEEERLRWVRQNQTVLRTDLYRNVCDAVVRGDTIAAATGKRIV 368
Query: 940 ----XXXXXXXXXXXXXXXXXXCR*VGPPSLFITFT*NPNWPEIKEVIEK-SGQKAADRP 1104
CR G P +F+TFT NP WPEI+ +++K GQ DRP
Sbjct: 369 LPSSFTGGPRYMVQNYQDAMAICRTFGNPDIFMTFTANPKWPEIQYMLQKIPGQSVDDRP 428
Query: 1105 DTISRVFMMKLDELMKDLRHGRQFDLVNAGLIASMYQTR 1221
D +RVF MKLD+LMK + G+ F + + + +Q R
Sbjct: 429 DIKTRVFKMKLDQLMKHIVEGQYFGKIISAIYTIEFQKR 467
Score = 95.1 bits (235), Expect(2) = 5e-46
Identities = 52/101 (51%), Positives = 67/101 (66%), Gaps = 8/101 (7%)
Frame = +3
Query: 504 AAIMVGD----GSGRDIIVDRKDSGFQRISQVYRKFMAMQYPILFPYGEDGFTVDIPYSS 671
A ++VGD RDI+++ K G QRI + + KFMAMQYP+LFPY EDGF +DI YS
Sbjct: 215 AGLIVGDFGESNGRRDIVIEHKTDGLQRIKEYHPKFMAMQYPLLFPYAEDGFHLDIYYS- 273
Query: 672 VQNNVHRKK----KTVSKREHYAYRLQERVNEGQTLILDWR 782
QN+ RKK K V+ RE+YAYR+Q+R E TLI+ R
Sbjct: 274 -QNHAKRKKKKKRKKVTLREYYAYRIQQRPCEANTLIIGGR 313
>ref|XP_003609124.1| ATP-dependent DNA helicase PIF1 [Medicago truncatula]
gi|355510179|gb|AES91321.1| ATP-dependent DNA helicase
PIF1 [Medicago truncatula]
Length = 1558
Score = 119 bits (298), Expect(2) = 8e-46
Identities = 65/159 (40%), Positives = 89/159 (55%), Gaps = 9/159 (5%)
Frame = +1
Query: 772 LIGGRLRQQFVVDAYACMEEARLWWYRNNQAMVRAELYKGLVDVVVAGDTTPASVG---- 939
+IGGRL QQ+VVDA+ +EE RL W R NQ ++R +LY+ + D VV GDT A+ G
Sbjct: 309 IIGGRLFQQYVVDAFTAIEEERLRWVRQNQTVLRTDLYRNVCDAVVRGDTIAAATGKRIV 368
Query: 940 ----XXXXXXXXXXXXXXXXXXCR*VGPPSLFITFT*NPNWPEIKEVIEK-SGQKAADRP 1104
CR G P +F+TFT NP WPEI+ +++K GQ DRP
Sbjct: 369 LPSSFTGGPRYMVQNYQDAMAICRTFGNPDIFMTFTANPKWPEIQYMLQKIPGQSVDDRP 428
Query: 1105 DTISRVFMMKLDELMKDLRHGRQFDLVNAGLIASMYQTR 1221
D +RVF MKLD+LMK + G+ F + + + +Q R
Sbjct: 429 DIKTRVFKMKLDQLMKHIVEGQYFGKIISAIYTIEFQKR 467
Score = 94.4 bits (233), Expect(2) = 8e-46
Identities = 50/93 (53%), Positives = 65/93 (69%), Gaps = 5/93 (5%)
Frame = +3
Query: 519 GDGSGR-DIIVDRKDSGFQRISQVYRKFMAMQYPILFPYGEDGFTVDIPYSSVQNNVHRK 695
G+ +GR DI+++ K G QRI + + KFMAMQYP+LFPY EDGF +DI YS QN+ RK
Sbjct: 223 GESNGRRDIVIEHKTDGLQRIKEYHPKFMAMQYPLLFPYAEDGFHLDIYYS--QNHAKRK 280
Query: 696 K----KTVSKREHYAYRLQERVNEGQTLILDWR 782
K K V+ RE+YAYR+Q+R E TLI+ R
Sbjct: 281 KKKKRKKVTMREYYAYRIQQRPCEANTLIIGGR 313
>ref|XP_003609074.1| ATP-dependent DNA helicase PIF1 [Medicago truncatula]
gi|355510129|gb|AES91271.1| ATP-dependent DNA helicase
PIF1 [Medicago truncatula]
Length = 1531
Score = 119 bits (298), Expect(2) = 8e-46
Identities = 65/159 (40%), Positives = 89/159 (55%), Gaps = 9/159 (5%)
Frame = +1
Query: 772 LIGGRLRQQFVVDAYACMEEARLWWYRNNQAMVRAELYKGLVDVVVAGDTTPASVG---- 939
+IGGRL QQ+VVDA+ +EE RL W R NQ ++R +LY+ + D VV GDT A+ G
Sbjct: 282 IIGGRLFQQYVVDAFTAIEEERLRWVRQNQTVLRTDLYRNVCDAVVRGDTIAAATGKRIV 341
Query: 940 ----XXXXXXXXXXXXXXXXXXCR*VGPPSLFITFT*NPNWPEIKEVIEK-SGQKAADRP 1104
CR G P +F+TFT NP WPEI+ +++K GQ DRP
Sbjct: 342 LPSSFTGGPRYMVQNYQDAMAICRTFGNPDIFMTFTANPKWPEIQYMLQKIPGQSVDDRP 401
Query: 1105 DTISRVFMMKLDELMKDLRHGRQFDLVNAGLIASMYQTR 1221
D +RVF MKLD+LMK + G+ F + + + +Q R
Sbjct: 402 DIKTRVFKMKLDQLMKHIVEGQYFGKIISAIYTIEFQKR 440
Score = 94.4 bits (233), Expect(2) = 8e-46
Identities = 50/93 (53%), Positives = 65/93 (69%), Gaps = 5/93 (5%)
Frame = +3
Query: 519 GDGSGR-DIIVDRKDSGFQRISQVYRKFMAMQYPILFPYGEDGFTVDIPYSSVQNNVHRK 695
G+ +GR DI+++ K G QRI + + KFMAMQYP+LFPY EDGF +DI YS QN+ RK
Sbjct: 196 GESNGRRDIVIEHKTDGLQRIKEYHPKFMAMQYPLLFPYAEDGFHLDIYYS--QNHAKRK 253
Query: 696 K----KTVSKREHYAYRLQERVNEGQTLILDWR 782
K K V+ RE+YAYR+Q+R E TLI+ R
Sbjct: 254 KKKKRKKVTMREYYAYRIQQRPCEANTLIIGGR 286
>gb|AAK54302.1|AC034258_20 putative helicase [Oryza sativa Japonica Group]
gi|31432486|gb|AAP54108.1| AT hook motif-containing
protein, putative [Oryza sativa Japonica Group]
Length = 1573
Score = 115 bits (289), Expect(3) = 5e-44
Identities = 63/156 (40%), Positives = 84/156 (53%), Gaps = 9/156 (5%)
Frame = +1
Query: 781 GRLRQQFVVDAYACMEEARLWWYRNNQAMVRAELYKGLVDVVVAGDTTPASVG------- 939
G L QF+VDAY C+E+ RL W R NQ ++R ELY GL D + GDT +G
Sbjct: 475 GNLSMQFMVDAYTCIEQCRLSWIRQNQGILRTELYGGLQDALRTGDTRTEKLGRRIVLPA 534
Query: 940 -XXXXXXXXXXXXXXXXXXCR*VGPPSLFITFT*NPNWPEIKEVIEKSG-QKAADRPDTI 1113
CR G P LF+TFT NP WPEI+ ++EK G QK ++RPD +
Sbjct: 535 SFTGGPRNKEQNYQDAMAICRWAGNPDLFVTFTCNPKWPEIQCMLEKVGHQKPSERPDIV 594
Query: 1114 SRVFMMKLDELMKDLRHGRQFDLVNAGLIASMYQTR 1221
RVFM+KL ELM D++ + F A + +Q +
Sbjct: 595 VRVFMIKLRELMSDIKRNQHFGKTKAIIFTIEFQKK 630
Score = 85.5 bits (210), Expect(3) = 5e-44
Identities = 41/75 (54%), Positives = 57/75 (76%)
Frame = +3
Query: 531 GRDIIVDRKDSGFQRISQVYRKFMAMQYPILFPYGEDGFTVDIPYSSVQNNVHRKKKTVS 710
GRDI+++ KD +RIS+ + KFMAMQYP+LFPYGEDG+ + I YS ++ V KK V+
Sbjct: 394 GRDIVLEYKDMRPKRISETHPKFMAMQYPLLFPYGEDGYRLGIKYSG-KDGVRYDKKCVT 452
Query: 711 KREHYAYRLQERVNE 755
RE+YAYRLQ+R ++
Sbjct: 453 MREYYAYRLQQRQDQ 467
Score = 26.9 bits (58), Expect(3) = 5e-44
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = +2
Query: 440 LREAFRMARDRFREDE 487
L + FRMAR+RF+ED+
Sbjct: 338 LAQTFRMARERFKEDD 353