BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00006516
         (2491 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf...   614   e-173
ref|XP_004145402.1| PREDICTED: RING-H2 finger protein ATL65-like...   299   3e-78
ref|XP_002281140.2| PREDICTED: RING-H2 finger protein ATL65-like...   291   6e-76
ref|XP_003541102.1| PREDICTED: RING-H2 finger protein ATL65-like...   278   5e-72
ref|XP_003539029.1| PREDICTED: RING-H2 finger protein ATL65-like...   278   7e-72

>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT; AltName:
            Full=Tetrahydrocolumbamine 2-O-methyltransferase
            gi|24210466|dbj|BAC22084.1| columbamine
            O-methyltransferase [Coptis japonica]
          Length = 351

 Score =  614 bits (1583), Expect = e-173
 Identities = 313/362 (86%), Positives = 322/362 (88%)
 Frame = -1

Query: 2416 MDTPNTFQNDDEIKAQAEVWKHMYGFAKTIMLRSTISLGIPDIIHNNGPVTLSQLVTHLP 2237
            MDTPNTFQNDDEIKAQA+VWKHM+GFA+TIMLRST+SLGIPDIIHNNGPVTLSQLVTHLP
Sbjct: 1    MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP 60

Query: 2236 LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT 2057
            LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT
Sbjct: 61   LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT 120

Query: 2056 HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPEMNELLNDAMTSHSTFMLPALVS 1877
            HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPE+NE+LNDAMTSHSTFMLPALVS
Sbjct: 121  HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVS 180

Query: 1876 GLMKENVLDGVASIVDVGGNSGVVAKAIVDAFPDVKCSVMDLNHVIESVIKDPKLDYVAG 1697
            GLMKENVLDGVASIVDVGGNSGVVAK IVDAFP VKCSVMDLNHVIE VIK+PKLDYVAG
Sbjct: 181  GLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAG 240

Query: 1696 DXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDDCIKILNKAKEALPSTGGKVILVEI 1517
            D                                 DDCIKILN AKEALPSTGGKVILVEI
Sbjct: 241  D-----------MFTSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEI 289

Query: 1516 VVDTENLPLFTSARLSMDVDMMLMGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVA 1337
            VVDTENLPLFTSARLSM +DMMLM GKERTKKEWEDLLRKA+FT HQVIPIMAIESIIVA
Sbjct: 290  VVDTENLPLFTSARLSMGMDMMLMSGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVA 349

Query: 1336 YS 1331
            YS
Sbjct: 350  YS 351


>ref|XP_004145402.1| PREDICTED: RING-H2 finger protein ATL65-like [Cucumis sativus]
            gi|449472703|ref|XP_004153673.1| PREDICTED: RING-H2
            finger protein ATL65-like [Cucumis sativus]
            gi|449532533|ref|XP_004173235.1| PREDICTED: RING-H2
            finger protein ATL65-like [Cucumis sativus]
          Length = 421

 Score =  299 bits (765), Expect = 3e-78
 Identities = 198/389 (50%), Positives = 238/389 (61%), Gaps = 43/389 (11%)
 Frame = +3

Query: 90   PTPS---PSRLPIDFSPPLXXXXXXXXXXXXXXXXXRLIXXXXXXXXXXXXXXXXXXXXX 260
            P PS   P++LP+DFSPPL                 RLI                     
Sbjct: 38   PPPSSLQPAKLPVDFSPPLIAMVVVIATAFLIVTYSRLISRHLIPPILHLLRLHRRRWRR 97

Query: 261  XXXXXXX-----------GDIESNLSNISNFIPDNSTFHYFSSYNGLGEDVIKTIPLSIY 407
                              GD++S   +   F P    FH FS Y GL E VIKTIPLS+Y
Sbjct: 98   WRRRRRLAARSYIPSSSGGDLDSLPYDSPFFEPTTDAFHVFSPY-GLDESVIKTIPLSVY 156

Query: 408  TKISKTSHDCAVCLVEFEENDYVRTLPICSHAFHVECIDIWLTTHANCPLCRAGIFRLES 587
            T  S+   +CAVCL+EFE++DYVRTLPICSHAFHV+CID+WL +HANCPLCRA +FR ES
Sbjct: 157  TAKSR-DRECAVCLLEFEDDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPES 215

Query: 588  ----LMASRIRPSLDG-ILESFLSETRSV-----------IQETEIS---DQVSSQRITE 710
                +MA+RIRPSLD  IL S + E  +              E+EI+   D+  +    E
Sbjct: 216  PFTPVMAARIRPSLDDTILRSIILEPLAEGPPIPNRDSYHATESEITPCIDEPRNSNSVE 275

Query: 711  TEERSRNNVLKRSYSFGFERSLATTDRIVLEAVTATPWRYRRGNHLWTKRHPSPF--LGK 884
             +   R+ +LKRSYSFGFERSLA ++R++LE  T +PWRYRRG+  W+KR PSPF  L K
Sbjct: 276  DQMNGRDFLLKRSYSFGFERSLA-SERMILEPATTSPWRYRRGS-FWSKR-PSPFGSLPK 332

Query: 885  GSRVFSFR--RGMKSPFIFRRRHGFFPLSESNARYHG--GGGSSRRTKSMTMASPMFMRS 1052
             +RVFSFR  RGMKSPF FRRR GFFPLSES+ R+    GGGSSRR+KSMT  SPMF+RS
Sbjct: 333  -ARVFSFRYYRGMKSPF-FRRRGGFFPLSESSWRFSNGVGGGSSRRSKSMT--SPMFLRS 388

Query: 1053 T----GGNAAFSSSRLRCGDLEALLSPER 1127
            +    GG AAFSSSRLRCGD EALLSPER
Sbjct: 389  SVTGGGGAAAFSSSRLRCGDPEALLSPER 417


>ref|XP_002281140.2| PREDICTED: RING-H2 finger protein ATL65-like [Vitis vinifera]
          Length = 396

 Score =  291 bits (745), Expect = 6e-76
 Identities = 200/402 (49%), Positives = 239/402 (59%), Gaps = 42/402 (10%)
 Frame = +3

Query: 48   MLPVNSSHNQIDWGPTPS-------------PSRLPIDFSPPLXXXXXXXXXXXXXXXXX 188
            M+P     ++   GP+PS             PS++P+DFSPPL                 
Sbjct: 4    MVPSKPPEHEFRLGPSPSSFVSTSDTNDYQSPSKVPVDFSPPLIAMVVVVATAFLIVTYS 63

Query: 189  RLIXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----GDIESNLSNISNFIPDNSTFHYFS 353
            RLI                                 GDIES   N S        +  FS
Sbjct: 64   RLISRHLLPPFLRLLRSWRRWHRRRRRRRSHMPSSAGDIESPPYNGS--FDATEAYQVFS 121

Query: 354  SYNGLGEDVIKTIPLSIYTKISKTSH-------DCAVCLVEFEENDYVRTLPICSHAFHV 512
             Y GL E VIKTIPLS+YT    + H       DCAVCL+EFE+ND VR LP+CSHAFHV
Sbjct: 122  PY-GLDESVIKTIPLSLYTAKGLSKHGFVDDSRDCAVCLLEFEDNDCVRVLPVCSHAFHV 180

Query: 513  ECIDIWLTTHANCPLCRAGIFRLES----LMASRIRPSL-DGILESFLSE------TRSV 659
            +CIDIWL +HANCPLCRAGIFR ES    +MA+RIRPSL D ILES L E        S 
Sbjct: 181  DCIDIWLRSHANCPLCRAGIFRHESPFIPVMAARIRPSLDDAILESILLEPLSEAAPESE 240

Query: 660  IQETEISDQVSSQRITETEER--SRNNVLKRSYSFGFERSLATTDRIVLEAVTATPWRYR 833
               TEI     S R  ++E+R  +R+ +LKRSYSFGFER+   ++R+V+E  TA+PWRYR
Sbjct: 241  TTVTEIVPAEPSPRRNQSEDRFNARDFMLKRSYSFGFERNF-PSERLVMEPATASPWRYR 299

Query: 834  RGNHLWTKRHPSPF--LGKGSRVFSFR--RGMKSPFIFRRRHGFFPLSESNARYHGGGGS 1001
            RG+  W+KR PSPF  L K SRVFSFR  RGMKSPF FRRR  FFPLSES+AR+  GGGS
Sbjct: 300  RGS-FWSKR-PSPFGSLTK-SRVFSFRYYRGMKSPF-FRRRGSFFPLSESHARF-SGGGS 354

Query: 1002 SRRTKSMTMASPMFMRSTGGNAAFSSSRLRCGDLEALLSPER 1127
            SRRTKS+T  SP+F+RS+    A +S+RLRC DLEALLSPER
Sbjct: 355  SRRTKSLT--SPIFIRSSA--TASASTRLRCRDLEALLSPER 392


>ref|XP_003541102.1| PREDICTED: RING-H2 finger protein ATL65-like [Glycine max]
          Length = 393

 Score =  278 bits (711), Expect = 5e-72
 Identities = 185/399 (46%), Positives = 228/399 (57%), Gaps = 51/399 (12%)
 Frame = +3

Query: 84   WGPTPSP----SRLP-------IDFSPPLXXXXXXXXXXXXXXXXXRLIXXXXXXXXXXX 230
            W P+P+P    S LP       +DF+PPL                 RLI           
Sbjct: 10   WAPSPTPILSKSFLPPTSNSPAVDFTPPLIAMVVVVAAAFLVVTYSRLIARHLRPPVHRL 69

Query: 231  XXXXXXXXXXXXXXXXXGDIESNLSNISNFIPDNSTF---HYFSSYNGLGEDVIKTIPLS 401
                             GD+ES        +P +S F   H FS Y GL E VIKTIP S
Sbjct: 70   LRRFRRRRFLPSSV---GDLES--------LPYDSPFDGPHVFSPY-GLDETVIKTIPFS 117

Query: 402  IYT-----KISKTSHDCAVCLVEFEENDYVRTLPICSHAFHVECIDIWLTTHANCPLCRA 566
            +YT     +  ++ +DCAVCL+EFE+ DYVRTLP+CSH FHV+CID WL +HANCPLCRA
Sbjct: 118  LYTAKYDARFDESRYDCAVCLLEFEDEDYVRTLPVCSHTFHVDCIDAWLRSHANCPLCRA 177

Query: 567  GIFRLES----LMASRIRPSLD----------------------GILESFLSETRSVIQE 668
            G+   +S    +MA+RIRPSLD                       +  S +SE    + E
Sbjct: 178  GVLCTDSPFTPMMAARIRPSLDDDTILHRISIDPLIDLPPQPASAVAISSVSEITPCVDE 237

Query: 669  TEISDQVSSQRITETEERSRNNVLKRSYSFGFERSLATTDRIVLEAVTATPWRYRRGNHL 848
                   +   +  +E+  R+ +LKRSYSFGFERSLA ++R+V+E  TA+PWRYRRG+  
Sbjct: 238  NTPRRNQNHANV-NSEDCFRDFLLKRSYSFGFERSLA-SERMVMEPATASPWRYRRGSSS 295

Query: 849  WTKRHPSPF--LGKGSRVFSFR--RGMKSPFIFRRRHGFFPLSESNARYHGGGGSSRRTK 1016
            +  + PSPF  LGK  RVFSFR  RGMKSPF   RR GFFPLSES+ RY  GG SSRR+K
Sbjct: 296  FWSKRPSPFGSLGK-PRVFSFRYYRGMKSPFF--RRRGFFPLSESSVRYGSGGSSSRRSK 352

Query: 1017 SMTMASPMFMRSTG--GNAAFSSSRLRCGDLEALLSPER 1127
            S  +ASPMF+RS+G    AAFSSSRLRCGD EALLSPER
Sbjct: 353  S--IASPMFLRSSGVAAAAAFSSSRLRCGDPEALLSPER 389


>ref|XP_003539029.1| PREDICTED: RING-H2 finger protein ATL65-like [Glycine max]
          Length = 399

 Score =  278 bits (710), Expect = 7e-72
 Identities = 187/386 (48%), Positives = 230/386 (59%), Gaps = 42/386 (10%)
 Frame = +3

Query: 96   PSPSRLP-IDFSPPLXXXXXXXXXXXXXXXXXRLIXXXXXXXXXXXXXXXXXXXXXXXXX 272
            P PS  P +DFSPPL                 RLI                         
Sbjct: 28   PPPSNSPAVDFSPPLIAMVVVVAAAFLVVTYSRLIARHLRPPIHRLLRRFRRRRFLPSSV 87

Query: 273  XXXGDIESNLSNISNFIPDNSTF---HYFSSYNGLGEDVIKTIPLSIYT-----KISKTS 428
               GD+ES        +P  S F   H FS Y GL E VIKTIP S+YT     +  ++ 
Sbjct: 88   ---GDLES--------LPYESPFDGPHVFSPY-GLDETVIKTIPFSLYTAKYDARFDESR 135

Query: 429  HDCAVCLVEFEENDYVRTLPICSHAFHVECIDIWLTTHANCPLCRAGIFRLES----LMA 596
            +DCAVCL+EFE++DYVRTLPICSH FHV+CID WL +HANCPLCRAG+   +S    +MA
Sbjct: 136  NDCAVCLLEFEDDDYVRTLPICSHTFHVDCIDAWLRSHANCPLCRAGVLCTDSPFTPMMA 195

Query: 597  SRIRP-----------SLDGILESFLSETRSVIQETEIS---DQVSSQR-----ITETEE 719
            +RIRP           S+D +++  L    ++    EI+   D+ S +R        +E+
Sbjct: 196  ARIRPSFDDDTILHRISIDPLIDPPLLPAAAISSVPEITPCVDENSPRRNQNHANVNSED 255

Query: 720  RSRNNVLKRSYSFGFERSLATTDRIVLEAVTATPWRYRRGNHLWTKRHPSPF--LGKGSR 893
              R+ +LKRSYSFGFERSLA ++R+V+E  TA+PWRYRRG+  +  + PSPF  LGK  R
Sbjct: 256  CFRDFLLKRSYSFGFERSLA-SERMVMEPATASPWRYRRGSSSFWSKRPSPFGSLGK-PR 313

Query: 894  VFSFR--RGMKSPFIFRRRHGFFPLSESNARYHGGGG----SSRRTKSMTMASPMFMRST 1055
            VFSFR  RGMKSPF   RR GFFPLSES+ RY GGGG    SSRR+KS  +ASPMF+RS+
Sbjct: 314  VFSFRYYRGMKSPFF--RRRGFFPLSESSVRYGGGGGGGGSSSRRSKS--IASPMFLRSS 369

Query: 1056 G--GNAAFSSSRLRCGDLEALLSPER 1127
            G    AAFSSSRLRCGD EALLSPER
Sbjct: 370  GVTSAAAFSSSRLRCGDPEALLSPER 395