BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00006432
         (3530 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268884.1| PREDICTED: tRNA wybutosine-synthesizing prot...  1221   0.0  
emb|CBI23243.3| unnamed protein product [Vitis vinifera]             1219   0.0  
ref|XP_003529632.1| PREDICTED: tRNA wybutosine-synthesizing prot...  1179   0.0  
ref|XP_004155603.1| PREDICTED: tRNA wybutosine-synthesizing prot...  1179   0.0  
ref|XP_003610434.1| tRNA wybutosine-synthesizing protein 2/3/4 [...  1174   0.0  

>ref|XP_002268884.1| PREDICTED: tRNA wybutosine-synthesizing protein 2/3/4-like [Vitis
            vinifera]
          Length = 1018

 Score = 1221 bits (3159), Expect = 0.0
 Identities = 644/1048 (61%), Positives = 765/1048 (72%), Gaps = 14/1048 (1%)
 Frame = -3

Query: 3516 MEFEKRKAATLTSISSAELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISILLQP 3337
            MEFEKRKAATL S+ S E DKSPKGTID  I+PLL  IN H SYFTTSSCSGRISIL QP
Sbjct: 1    MEFEKRKAATLASMGSTEPDKSPKGTIDRHIVPLLSAINRHNSYFTTSSCSGRISILSQP 60

Query: 3336 LPQXXXXXXXXXXXXXKWVFITHELAKANIVIDLLFPPNK-STEDGELVFRFEPFILAVE 3160
             P               W+FITH+LA  N V+ LLFP    S++  +LVFRFEPFI+AVE
Sbjct: 61   SPAATNAHKKARGGS--WLFITHDLADPNSVLALLFPTTGCSSQHDDLVFRFEPFIVAVE 118

Query: 3159 CKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPFGTVGNVMVSKEYVE 2980
            CKDV+AAQ LV+TA+SCGFRESGITSV+ KRV++A+RCSIRLEVP G  G V+VS EYV 
Sbjct: 119  CKDVAAAQLLVSTAVSCGFRESGITSVS-KRVMVAVRCSIRLEVPLGGGGRVLVSPEYVR 177

Query: 2979 YLVGIANEKMESNRKRTEGFLQVLESE-----MMGREEKDGCCG-EENGNVDSRQMAVGE 2818
            YLVGIAN+KME+NR+RTEGFLQ L+S        G    DG  G +E+G  D +    G+
Sbjct: 178  YLVGIANDKMETNRRRTEGFLQALQSSGFVESFNGGAGLDGAMGGDEHGCSDCKD---GD 234

Query: 2817 CDLGREDSNAQSGLLNAHWGSISAVKILTIGEPVDKLFLWGHSACTLYNSLQQKVLIFGG 2638
             +  R  +  +SG +     S+S V++  I EPV+KLFLWGHSACTL     +KV++FGG
Sbjct: 235  ANSERIIAEKESGSVGVLGCSLSIVQMEIIDEPVEKLFLWGHSACTLDTINHKKVIVFGG 294

Query: 2637 FGGLGRHARRNDTLTLDLQSGALKVISVEGPPSPRLGHTSSVVGEHVFVIGGRGDPTKIL 2458
            FGG+GRHARRND   LD   G LK ++ EG PSPRLGHTSS+VG+ +F+IGGR DP  IL
Sbjct: 295  FGGMGRHARRNDAFVLDPLHGTLKAVNAEGTPSPRLGHTSSMVGDLMFIIGGRADPENIL 354

Query: 2457 NDVWVFNTSKNQWKLLECSGSVFLPRHRHAAAVVGSKIYVFGGLNNEIIYSSMYVLDSEN 2278
            ++VWV +T+KN+W+ LEC+GSVF PRHRHAAAV+GSKIYVFGGLNN+ I SS++VLD++N
Sbjct: 355  DNVWVLDTAKNEWRRLECTGSVFPPRHRHAAAVLGSKIYVFGGLNNDAISSSLHVLDTDN 414

Query: 2277 SRWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIACLWKKEKTAG 2098
             +W+EI   GE PC+RHSH+LVA  S+LFMFGG +  K   DLY FD   CLWKKE  +G
Sbjct: 415  LQWNEIRVHGEWPCARHSHSLVAYGSKLFMFGGCNDGKALGDLYSFDVQTCLWKKEVASG 474

Query: 2097 TPPLAKFSHSMFIYKNYLGILGGCPVIQNNQEMSLLDLRIGLWKHVILNTVSKELFVRST 1918
              P A+FSHSMFIYKNYLGI+GGCPV Q+ QE++LLDL+  +W++ IL++V K LFVRST
Sbjct: 475  RTPYARFSHSMFIYKNYLGIIGGCPVRQHCQELALLDLQHHVWRNEILDSVFKVLFVRST 534

Query: 1917 XXXXXXXXXXXXXGASCYAFGTKFSLPMKISLLPYVSFSDT-VTLHSKEMPSRHHPETVL 1741
                         GASCYAFGTKFS PMKI+LL  VS  DT V    +E  + H  E V 
Sbjct: 535  ASVVGDDLIMIGGGASCYAFGTKFSGPMKINLLQLVSLHDTLVPSEMEEKHAIHQYEGVK 594

Query: 1740 ENITGSVFFTAKNVQTLTKSCISNYKTEVLGESFDIEQGATHWVLRLERKYAKLGKDILK 1561
            E   G +    +                        +  A +WVL+LERKYAKLGKDILK
Sbjct: 595  EKKNGDLHVDVEK-----------------------QMVAVYWVLQLERKYAKLGKDILK 631

Query: 1560 KFGWLDLRRKVYSSENGSHICLPVTEKFYPLFHKKQFNLMDSNDGLSTPCKLVNIEELST 1381
            KFGWLDL RKV+S E+  HIC PVTEKF  +F++K     DS+D    P +L ++ + ST
Sbjct: 632  KFGWLDLGRKVHSREDRRHICFPVTEKFCTIFNEKDH---DSSDAFEVPNEL-HLYKPST 687

Query: 1380 ------YDFTCEMALDNLFLLGGLIVKDEVACERIAPKSPQKVMSEAVESLIMKMGLPAR 1219
                   D +   AL  L   G   + DEV   R  P SP K+MSEAV SLI   GL ++
Sbjct: 688  GEGVLLNDISFVTALRLLKECGATKLADEVVEVRRTPSSPLKIMSEAVASLIKHRGLSSQ 747

Query: 1218 LLEEVPTRWERLGDIVVLPVNSFRDASWNSIGQELWPTVAKSLGTRRLARQGRVASTGTR 1039
            LLE++PTRWERLGDIVVLPV SF+D  W+SIG ELWP +AKSL T RLARQGRVA +GTR
Sbjct: 748  LLEQLPTRWERLGDIVVLPVTSFKDPIWDSIGDELWPIIAKSLNTCRLARQGRVAPSGTR 807

Query: 1038 DSTLDILVGDDGWVDHRENGIMYSFDATKCMFSWGNLSEKLRMACLDCKGEVIVDLFAGI 859
            DSTL+ILVGD+GWVDH ENGI+YSFDATKCMFSWGNLSEKLRM CL+C+ EVIVDLFAGI
Sbjct: 808  DSTLEILVGDNGWVDHCENGILYSFDATKCMFSWGNLSEKLRMGCLNCRDEVIVDLFAGI 867

Query: 858  GYFVLPFLVRAKAKMVYACEWNPHALEALQRNVESNSVGDRCVILEGDNRATAPKGVADR 679
            GYFVLPFLV AKAK+VYACEWNPHA+EALQ N+ +NSV DRC+ILEGDNR TAPKGVADR
Sbjct: 868  GYFVLPFLVSAKAKLVYACEWNPHAVEALQHNLLANSVADRCIILEGDNRLTAPKGVADR 927

Query: 678  VCLGLLPSSEGSWVTAVRALRMEGGLLHVHGIAKDSEESIWSDHVANSIQSIAKSEGLCW 499
            VCLGLLPSSEGSW TAVRALR EGG+LHVHG AKDSEE  WS+HV+ SI  +A+SEG  W
Sbjct: 928  VCLGLLPSSEGSWATAVRALRTEGGMLHVHGNAKDSEEGSWSEHVSKSICDLARSEGYDW 987

Query: 498  EVSVQHLERVKWYGPHIRHLVLDVRCKQ 415
            EVSV+H+ERVKWY PHIRHLV DVRC+Q
Sbjct: 988  EVSVEHVERVKWYAPHIRHLVADVRCRQ 1015


>emb|CBI23243.3| unnamed protein product [Vitis vinifera]
          Length = 1013

 Score = 1219 bits (3153), Expect = 0.0
 Identities = 642/1047 (61%), Positives = 766/1047 (73%), Gaps = 13/1047 (1%)
 Frame = -3

Query: 3516 MEFEKRKAATLTSISSAELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISILLQP 3337
            MEFEKRKAATL S+ S E DKSPKGTID  I+PLL  IN H SYFTTSSCSGRISIL QP
Sbjct: 1    MEFEKRKAATLASMGSTEPDKSPKGTIDRHIVPLLSAINRHNSYFTTSSCSGRISILSQP 60

Query: 3336 LPQXXXXXXXXXXXXXKWVFITHELAKANIVIDLLFPPNK-STEDGELVFRFEPFILAVE 3160
             P               W+FITH+LA  N V+ LLFP    S++  +LVFRFEPFI+AVE
Sbjct: 61   SPAATNAHKKARGGS--WLFITHDLADPNSVLALLFPTTGCSSQHDDLVFRFEPFIVAVE 118

Query: 3159 CKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPFGTVGNVMVSKEYVE 2980
            CKDV+AAQ LV+TA+SCGFRESGITSV+ KRV++A+RCSIRLEVP G  G V+VS EYV 
Sbjct: 119  CKDVAAAQLLVSTAVSCGFRESGITSVS-KRVMVAVRCSIRLEVPLGGGGRVLVSPEYVR 177

Query: 2979 YLVGIANEKMESNRKRTEGFLQVLESE-----MMGREEKDGCCG-EENGNVDSRQMAVGE 2818
            YLVGIAN+KME+NR+RTEGFLQ L+S        G    DG  G +E+G  D +    G+
Sbjct: 178  YLVGIANDKMETNRRRTEGFLQALQSSGFVESFNGGAGLDGAMGGDEHGCSDCKD---GD 234

Query: 2817 CDLGREDSNAQSGLLNAHWGSISAVKILTIGEPVDKLFLWGHSACTLYNSLQQKVLIFGG 2638
             +  R  +  +SG +     S+S V++  I EPV+KLFLWGHSACTL     +KV++FGG
Sbjct: 235  ANSERIIAEKESGSVGVLGCSLSIVQMEIIDEPVEKLFLWGHSACTLDTINHKKVIVFGG 294

Query: 2637 FGGLGRHARRNDTLTLDLQSGALKVISVEGPPSPRLGHTSSVVGEHVFVIGGRGDPTKIL 2458
            FGG+GRHARRND   LD   G LK ++ EG PSPRLGHTSS+VG+ +F+IGGR DP  IL
Sbjct: 295  FGGMGRHARRNDAFVLDPLHGTLKAVNAEGTPSPRLGHTSSMVGDLMFIIGGRADPENIL 354

Query: 2457 NDVWVFNTSKNQWKLLECSGSVFLPRHRHAAAVVGSKIYVFGGLNNEIIYSSMYVLDSEN 2278
            ++VWV +T+KN+W+ LEC+GSVF PRHRHAAAV+GSKIYVFGGLNN+ I SS++VLD++N
Sbjct: 355  DNVWVLDTAKNEWRRLECTGSVFPPRHRHAAAVLGSKIYVFGGLNNDAISSSLHVLDTDN 414

Query: 2277 SRWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIACLWKKEKTAG 2098
             +W+EI   GE PC+RHSH+LVA  S+LFMFGG +  K   DLY FD   CLWKKE  +G
Sbjct: 415  LQWNEIRVHGEWPCARHSHSLVAYGSKLFMFGGCNDGKALGDLYSFDVQTCLWKKEVASG 474

Query: 2097 TPPLAKFSHSMFIYKNYLGILGGCPVIQNNQEMSLLDLRIGLWKHVILNTVSKELFVRST 1918
              P A+FSHSMFIYKNYLGI+GGCPV Q+ QE++LLDL+  +W++ IL++V K LFVRST
Sbjct: 475  RTPYARFSHSMFIYKNYLGIIGGCPVRQHCQELALLDLQHHVWRNEILDSVFKVLFVRST 534

Query: 1917 XXXXXXXXXXXXXGASCYAFGTKFSLPMKISLLPYVSFSDTVTLHSKEMPSRHHPETVLE 1738
                         GASCYAFGTKFS PMKI+LL  VS  DT+      +PS    +  + 
Sbjct: 535  ASVVGDDLIMIGGGASCYAFGTKFSGPMKINLLQLVSLHDTL------VPSEMEEKHAIH 588

Query: 1737 NITGSVFFTAKNVQTLTKSCISNYKTEVLGESFDIEQGATHWVLRLERKYAKLGKDILKK 1558
               G                +   K +V     + +  A +WVL+LERKYAKLGKDILKK
Sbjct: 589  QYEG----------------VKEKKNDV-----EKQMVAVYWVLQLERKYAKLGKDILKK 627

Query: 1557 FGWLDLRRKVYSSENGSHICLPVTEKFYPLFHKKQFNLMDSNDGLSTPCKLVNIEELST- 1381
            FGWLDL RKV+S E+  HIC PVTEKF  +F++K     DS+D    P +L ++ + ST 
Sbjct: 628  FGWLDLGRKVHSREDRRHICFPVTEKFCTIFNEKDH---DSSDAFEVPNEL-HLYKPSTG 683

Query: 1380 -----YDFTCEMALDNLFLLGGLIVKDEVACERIAPKSPQKVMSEAVESLIMKMGLPARL 1216
                  D +   AL  L   G   + DEV   R  P SP K+MSEAV SLI   GL ++L
Sbjct: 684  EGVLLNDISFVTALRLLKECGATKLADEVVEVRRTPSSPLKIMSEAVASLIKHRGLSSQL 743

Query: 1215 LEEVPTRWERLGDIVVLPVNSFRDASWNSIGQELWPTVAKSLGTRRLARQGRVASTGTRD 1036
            LE++PTRWERLGDIVVLPV SF+D  W+SIG ELWP +AKSL T RLARQGRVA +GTRD
Sbjct: 744  LEQLPTRWERLGDIVVLPVTSFKDPIWDSIGDELWPIIAKSLNTCRLARQGRVAPSGTRD 803

Query: 1035 STLDILVGDDGWVDHRENGIMYSFDATKCMFSWGNLSEKLRMACLDCKGEVIVDLFAGIG 856
            STL+ILVGD+GWVDH ENGI+YSFDATKCMFSWGNLSEKLRM CL+C+ EVIVDLFAGIG
Sbjct: 804  STLEILVGDNGWVDHCENGILYSFDATKCMFSWGNLSEKLRMGCLNCRDEVIVDLFAGIG 863

Query: 855  YFVLPFLVRAKAKMVYACEWNPHALEALQRNVESNSVGDRCVILEGDNRATAPKGVADRV 676
            YFVLPFLV AKAK+VYACEWNPHA+EALQ N+ +NSV DRC+ILEGDNR TAPKGVADRV
Sbjct: 864  YFVLPFLVSAKAKLVYACEWNPHAVEALQHNLLANSVADRCIILEGDNRLTAPKGVADRV 923

Query: 675  CLGLLPSSEGSWVTAVRALRMEGGLLHVHGIAKDSEESIWSDHVANSIQSIAKSEGLCWE 496
            CLGLLPSSEGSW TAVRALR EGG+LHVHG AKDSEE  WS+HV+ SI  +A+SEG  WE
Sbjct: 924  CLGLLPSSEGSWATAVRALRTEGGMLHVHGNAKDSEEGSWSEHVSKSICDLARSEGYDWE 983

Query: 495  VSVQHLERVKWYGPHIRHLVLDVRCKQ 415
            VSV+H+ERVKWY PHIRHLV DVRC+Q
Sbjct: 984  VSVEHVERVKWYAPHIRHLVADVRCRQ 1010


>ref|XP_003529632.1| PREDICTED: tRNA wybutosine-synthesizing protein 2/3/4-like [Glycine
            max]
          Length = 1068

 Score = 1179 bits (3051), Expect = 0.0
 Identities = 607/1050 (57%), Positives = 752/1050 (71%), Gaps = 12/1050 (1%)
 Frame = -3

Query: 3528 KNSKMEFEKRKAATLTSISSAELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISI 3349
            +  +MEFEKRKAATL S+SS E DKSPKG++D  I+PLL T+N +PSYFTTSSCSGRISI
Sbjct: 20   EKKRMEFEKRKAATLASLSSTESDKSPKGSLDAAIVPLLNTLNQNPSYFTTSSCSGRISI 79

Query: 3348 LLQPLPQXXXXXXXXXXXXXKWVFITHELAKANIVIDLLFPPNKSTED---GELVFRFEP 3178
            L QPL                W+F++H+ A  + V+ LLFP   +       ELVFRFEP
Sbjct: 80   LAQPLSLSDSPNPKKKARGGTWLFVSHDPADPDSVLSLLFPSESTPSPFAPSELVFRFEP 139

Query: 3177 FILAVECKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPFGTVGNVMV 2998
             I+A+EC+D+S+A SLV+ AISCGFRESGIT+   KR IIAIRCSIR+EVP G   NVMV
Sbjct: 140  LIIALECRDLSSAHSLVSLAISCGFRESGITNAK-KRFIIAIRCSIRMEVPLGDTRNVMV 198

Query: 2997 SKEYVEYLVGIANEKMESNRKRTEGFLQVLESEMMGREEKDGCCGEENGNVDSRQMAVGE 2818
            +  YV YLV +AN+KME+NRKRT+ F QVL S      +        N   D  ++  GE
Sbjct: 199  TPHYVRYLVQVANDKMEANRKRTQRFFQVLLSNGSVLADNSNHLSGTNEVCDHLELE-GE 257

Query: 2817 CDLGREDSNAQSGLL---NAHWGSISAVKILTIGEPVDKLFLWGHSACTLYNSLQQKVLI 2647
              L   +    SG++    +    +S      +GEPV+KL+ WGHSAC L ++  +KV++
Sbjct: 258  SQLENGNVGTSSGIIWTVGSPGCGLSIGHFEIVGEPVEKLYRWGHSACGLGDADHKKVIV 317

Query: 2646 FGGFGGLGRHARRNDTLTLDLQSGALKVISVEG--PPSPRLGHTSSVVGEHVFVIGGRGD 2473
            FGGFGG+GRHARRND L LD  SG L ++S  G   PSPRLGHT+S+VG  +FVIGGR  
Sbjct: 318  FGGFGGMGRHARRNDLLLLDPYSGNLDMVSTVGCASPSPRLGHTASLVGNRMFVIGGRTG 377

Query: 2472 PTKILNDVWVFNTSKNQWKLLECSGSVFLPRHRHAAAVVGSKIYVFGGLNNEIIYSSMYV 2293
            P KIL+DVW+ +T+KN W LL+C  S F PRHRHAAAV+GS IYVFGGL+N+II+SS YV
Sbjct: 378  PDKILSDVWILDTTKNSWNLLQCGDSGFPPRHRHAAAVMGSNIYVFGGLDNDIIFSSFYV 437

Query: 2292 LDSENSRWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIACLWKK 2113
             D+ N  W EI   G  PC+RHSHA+VA+DSQ+FMFGGY+G K   DL+ FD     W K
Sbjct: 438  FDTNNLHWKEIPVSGYWPCARHSHAMVASDSQIFMFGGYNGGKALGDLHSFDVQKGQWTK 497

Query: 2112 EKTAGTPPLAKFSHSMFIYKNYLGILGGCPVIQNNQEMSLLDLRIGLWKHVILNTVSKEL 1933
            E+TAG  P A+FSHS+F+YKNYLG+LGGCPV Q+ QE++LLDL++ LWKHV LN+V K+L
Sbjct: 498  ERTAGRNPHARFSHSIFLYKNYLGVLGGCPVRQHCQELALLDLKLRLWKHVTLNSVGKDL 557

Query: 1932 FVRSTXXXXXXXXXXXXXGASCYAFGTKFSLPMKISLLPYVSFSDTVTLHSKEMPSRHHP 1753
            FVRST             GASCYAFGTKFS P K+SLL  +   D      ++  S  + 
Sbjct: 558  FVRSTANVVGDDLAIVGGGASCYAFGTKFSEPAKVSLLHLMHSHDEPVKTQRKRTSNQNE 617

Query: 1752 ETVLENITGSVFFTAKNVQTLTKSCISNYKTEVLGESFDIEQGATHWVLRLERKYAKLGK 1573
             T   NI  S     ++   +++   S+    +   +   +  A H+VL+LE+KYAKLGK
Sbjct: 618  GTNRNNIENSCGPQLEHAPNISEDESSHSDDNIPCLNDQSQMIALHYVLQLEKKYAKLGK 677

Query: 1572 DILKKFGWLDLRRKVYSSENGSHICLPVTEKFYPLFHKKQFNLMDS----NDGLSTPCKL 1405
            DILKKFGWLDL RK YS E G HI  PV ++F+ +FH++  NL D+    N+GL +  K 
Sbjct: 678  DILKKFGWLDLGRKAYSDEGGVHIGFPVHQEFFAVFHERNHNLGDAFDRQNEGLFS--KP 735

Query: 1404 VNIEELSTYDFTCEMALDNLFLLGGLIVKDEVACERIAPKSPQKVMSEAVESLIMKMGLP 1225
            +  ++    + +C  AL  L   G +++ D+V  ER A KSP KVM+EA+ SLI   GLP
Sbjct: 736  LKRDKFLLNELSCSEALILLHEYGAIVLGDKVVEERKAAKSPLKVMTEAITSLIEHKGLP 795

Query: 1224 ARLLEEVPTRWERLGDIVVLPVNSFRDASWNSIGQELWPTVAKSLGTRRLARQGRVASTG 1045
            ARLLEE+PTRW++LGDIV+LP  SF+D+ W+SI +ELW  VAKSL   RLARQG VA+TG
Sbjct: 796  ARLLEELPTRWDQLGDIVLLPSTSFKDSMWDSIAEELWSIVAKSLKAHRLARQGPVAATG 855

Query: 1044 TRDSTLDILVGDDGWVDHRENGIMYSFDATKCMFSWGNLSEKLRMACLDCKGEVIVDLFA 865
            TRDSTL ILVGD+GWV+HRENGI+YSFDATKCMFSWGNLSEK+RMA LDCK EV+VDLFA
Sbjct: 856  TRDSTLQILVGDNGWVNHRENGILYSFDATKCMFSWGNLSEKIRMARLDCKDEVVVDLFA 915

Query: 864  GIGYFVLPFLVRAKAKMVYACEWNPHALEALQRNVESNSVGDRCVILEGDNRATAPKGVA 685
            GIGYFVLPFLVRA+AK+VYACEWNPHA+EALQ N+E+NSV DRC+ILEGDNR TAPK VA
Sbjct: 916  GIGYFVLPFLVRAQAKLVYACEWNPHAVEALQHNLEANSVADRCIILEGDNRITAPKSVA 975

Query: 684  DRVCLGLLPSSEGSWVTAVRALRMEGGLLHVHGIAKDSEESIWSDHVANSIQSIAKSEGL 505
            DRVCLGL+PSSE SWVTAVRALR EGG+LHVHG  +DSEES W DHV+ SI  IA+SEG 
Sbjct: 976  DRVCLGLIPSSELSWVTAVRALRREGGILHVHGNTRDSEESQWIDHVSKSIYDIARSEGY 1035

Query: 504  CWEVSVQHLERVKWYGPHIRHLVLDVRCKQ 415
             WEVS++H+ERVKWY PHIRH+V DVRC+Q
Sbjct: 1036 TWEVSIEHVERVKWYAPHIRHVVADVRCRQ 1065


>ref|XP_004155603.1| PREDICTED: tRNA wybutosine-synthesizing protein 2/3/4-like [Cucumis
            sativus]
          Length = 1035

 Score = 1179 bits (3049), Expect = 0.0
 Identities = 619/1059 (58%), Positives = 760/1059 (71%), Gaps = 25/1059 (2%)
 Frame = -3

Query: 3516 MEFEKRKAATLTSISSAELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISILLQP 3337
            MEFEKRKAAT+ S+ S E DKSPKG++DTPIIPL+ T+N+HPSYFTTSSCSGRISIL QP
Sbjct: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNSHPSYFTTSSCSGRISILAQP 60

Query: 3336 LP--QXXXXXXXXXXXXXKWVFITHELAKANIVIDLLF-PPNKSTEDGELVFRFEPFILA 3166
            +                  W+F++HE A+ N VIDLLF  P+ + E  ELVFRFEP I+A
Sbjct: 61   ISTTSAAAPKPKKKALGGSWLFVSHEFAEPNSVIDLLFRSPSTNRELSELVFRFEPLIIA 120

Query: 3165 VECKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPFGTVGNVMVSKEY 2986
            VECKD+ +AQ+LV+TAISCGFRESGITS + KRVIIAIRCSIR+EVP GT   +MV+ EY
Sbjct: 121  VECKDLGSAQALVSTAISCGFRESGITSAS-KRVIIAIRCSIRMEVPLGTSEKIMVTPEY 179

Query: 2985 VEYLVGIANEKMESNRKRTEGFLQVLESEMMGREEKDGCCG----EENGNV-DSRQMAVG 2821
            V+YLV +ANEKM +N+KRT+GFL+ L+S +        C G    E   NV DS      
Sbjct: 180  VQYLVNVANEKMVANKKRTDGFLKGLQSSISDASRT--CHGIPSREATENVNDSLDSKGH 237

Query: 2820 ECDLGREDSNAQSGLLNAHWG----SISAVKILTIGEPVDKLFLWGHSACTLYNSLQQKV 2653
            +C  G + +  + G+ NA+      S+S  +I   GEP++KLF+WGHSA T+++    KV
Sbjct: 238  DCADGDDGAALEGGVRNANSEACSYSLSVEQIDIAGEPIEKLFIWGHSATTIHD----KV 293

Query: 2652 LIFGGFGGLGRHARRNDTLTLDLQSGALKVISVEGPPSPRLGHTSSVVGEHVFVIGGRGD 2473
            + FGGFGG+GRHARRND L LD+ S  L+ I+VE  PSPRLGHTSS+VG+ ++V+GGR D
Sbjct: 294  IAFGGFGGMGRHARRNDLLLLDMLSYTLQTINVEDSPSPRLGHTSSLVGDRLYVVGGRTD 353

Query: 2472 PTKILNDVWVFNTSKNQWKLLECSGSVFLPRHRHAAAVVGSKIYVFGGLNNEIIYSSMYV 2293
            PT ILNDVW+FN ++ +W LLEC+GS F PRHRHAAA +GSKIYVFGGL N+ I SS   
Sbjct: 354  PTCILNDVWLFNITQEKWTLLECTGSPFSPRHRHAAAALGSKIYVFGGLENDRISSSFIF 413

Query: 2292 LDSENSRWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIACLWKK 2113
            LDS++ +W EI   GEQPC RHSH++V+  S ++MFGGYDGEK   DLY FDT AC WKK
Sbjct: 414  LDSDSHQWKEIQAGGEQPCGRHSHSMVSYGSHIYMFGGYDGEKTLGDLYSFDTNACYWKK 473

Query: 2112 EKTAGTPPLAKFSHSMFIYKNYLGILGGCPVIQNNQEMSLLDLRIGLWKHVILNTVSKEL 1933
            E  AGT P A+FSH+MF+YKNY+GI+GGCPV Q  QE++LLDL++  W+HV LN   +EL
Sbjct: 474  ENIAGTTPNARFSHAMFVYKNYIGIIGGCPVTQTYQELALLDLQLRCWRHVSLNCTGREL 533

Query: 1932 FVRSTXXXXXXXXXXXXXGASCYAFGTKFSLPMKISLLPYVSFSDTVTLHS---KEMPSR 1762
            FVRST             GASCYAFGT FS PMKI L P +S S+ V  HS   +++   
Sbjct: 534  FVRSTVSVVGNDLILVGGGASCYAFGTTFSEPMKIRLHPLIS-SEVVLGHSGNTEKLEKA 592

Query: 1761 HHPETVLENITGSVFFTAKNVQTLTKSCISNYKTEVLGESFDIEQGATHWVLRLERKYAK 1582
            +     + N          N Q+  ++   N   E    S + +QGA +WVL++ERKYAK
Sbjct: 593  NRDPKCMPN---------GNAQSFNEAFGFNIDFEK-SNSHEQKQGALYWVLQIERKYAK 642

Query: 1581 LGKDILKKFGWLDLRRKVYSSENGSHICLPVTEKFYPLFHKKQF----NLMDSND-GLST 1417
            L KDILKKFGWLD+ R V S  +G+HIC PV  KF   F +KQ      L   ND  +S 
Sbjct: 643  LVKDILKKFGWLDMGRNVSSRGSGTHICFPVNVKFCDTFDEKQSWWADQLEQENDFRISG 702

Query: 1416 P-----CKLVNIEELSTYDFTCEMALDNLFLLGGLIVKDEVACERIAPKSPQKVMSEAVE 1252
            P     C   N++           AL+ L   G   + DEV   + A K+P K MSEA+ 
Sbjct: 703  PESWEGCLTSNLK-----------ALNVLKKCGATKLVDEVVDIKTAAKTPFKKMSEAMS 751

Query: 1251 SLIMKMGLPARLLEEVPTRWERLGDIVVLPVNSFRDASWNSIGQELWPTVAKSLGTRRLA 1072
            SL+   GL   LLEE+PTRWERLGDIVVLPV SF+D +W++IG+ELWP VAKSLGT RLA
Sbjct: 752  SLLKHNGLSEELLEELPTRWERLGDIVVLPVTSFKDPTWDTIGEELWPLVAKSLGTYRLA 811

Query: 1071 RQGRVASTGTRDSTLDILVGDDGWVDHRENGIMYSFDATKCMFSWGNLSEKLRMACLDCK 892
            RQGRVASTGTRDS L+IL+GD+GWV+HRENGI YSFDATKCMFSWGNLSEKLRMA L+CK
Sbjct: 812  RQGRVASTGTRDSNLEILLGDNGWVEHRENGITYSFDATKCMFSWGNLSEKLRMAHLNCK 871

Query: 891  GEVIVDLFAGIGYFVLPFLVRAKAKMVYACEWNPHALEALQRNVESNSVGDRCVILEGDN 712
             E +VDLFAGIGYFVLPFLV AKAK+VYACEWNPHA+EAL+RN+++N V +RCV+LEGDN
Sbjct: 872  EETVVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVSNRCVVLEGDN 931

Query: 711  RATAPKGVADRVCLGLLPSSEGSWVTAVRALRMEGGLLHVHGIAKDSEESIWSDHVANSI 532
            R TAPKGVADRVCLGLLP+SEGSWVTAVRALR EGG LHVH   KDSEE  W+  + +SI
Sbjct: 932  RETAPKGVADRVCLGLLPTSEGSWVTAVRALRSEGGTLHVHDNVKDSEEEQWTQRLVHSI 991

Query: 531  QSIAKSEGLCWEVSVQHLERVKWYGPHIRHLVLDVRCKQ 415
              IAKSEG CW+++++H+ERVKWY PHIRHLV DV+CK+
Sbjct: 992  TEIAKSEGHCWDITIEHIERVKWYAPHIRHLVADVQCKR 1030


>ref|XP_003610434.1| tRNA wybutosine-synthesizing protein 2/3/4 [Medicago truncatula]
            gi|355511489|gb|AES92631.1| tRNA wybutosine-synthesizing
            protein 2/3/4 [Medicago truncatula]
          Length = 1046

 Score = 1174 bits (3036), Expect = 0.0
 Identities = 611/1061 (57%), Positives = 762/1061 (71%), Gaps = 27/1061 (2%)
 Frame = -3

Query: 3516 MEFEKRKAATLTSISSAELDKSPKGTIDTPIIPLLKTINNHPSYFTTSSCSGRISILLQP 3337
            MEFEKRK+ATL S++S E DKSPKG++DTPIIPL+ T+N +P+YFTTSSCSGRISIL QP
Sbjct: 1    MEFEKRKSATLASLNSTESDKSPKGSLDTPIIPLINTLNQNPNYFTTSSCSGRISILSQP 60

Query: 3336 LPQXXXXXXXXXXXXXKWVFITHELAKANIVIDLLFPPNK--STED--GELVFRFEPFIL 3169
            L                W+F++H  A  + +I LLFP +   ST+    ELVFRFEP I+
Sbjct: 61   LSPIPSPQTKKKAKGGTWLFVSHHTANPDSIISLLFPSDSTHSTQSPISELVFRFEPLII 120

Query: 3168 AVECKDVSAAQSLVATAISCGFRESGITSVNNKRVIIAIRCSIRLEVPFGTVGNVMVSKE 2989
            A+ECK++S+AQSLVA AIS GFRESGIT+ N KRVIIAIRCSIR+EVP G    +MV+ E
Sbjct: 121  AIECKELSSAQSLVALAISSGFRESGITNAN-KRVIIAIRCSIRMEVPLGDTQKIMVTPE 179

Query: 2988 YVEYLVGIANEKMESNRKRTEGFLQVLESEMMGREEKDGCCGEENGNVDSRQMAVG-ECD 2812
            YV+YLV +ANEKME+N  RT+ FL++L++        +G    +N N  S++  V   CD
Sbjct: 180  YVKYLVQVANEKMEANWNRTDRFLRLLQN--------NGSMVNDNSNRLSQRTGVELVCD 231

Query: 2811 LGREDSNAQ----------SGLLNAHWGSISAVKILTIGEPVDKLFLWGHSACTLYNSLQ 2662
              + D  +Q          SG + +   S+    I  +GEPV+KLFLWGHSAC L N+  
Sbjct: 232  NLQFDHESQITNGNAPKNESGFVGSPGFSLPIAHIEVVGEPVEKLFLWGHSACALDNADH 291

Query: 2661 QKVLIFGGFGGLGRHARRNDTLTLDLQSGALKVISVEGP--PSPRLGHTSSVVGEHVFVI 2488
            +KV++FGGFGGLGRHARRND L LD  S  L+ I   G   PSPRLGHT+S+VG+ +FVI
Sbjct: 292  KKVIVFGGFGGLGRHARRNDLLLLDPYSFNLETIDTSGCACPSPRLGHTASLVGDLMFVI 351

Query: 2487 GGRGDPTKILNDVWVFNTSKNQWKLLECSGSVFLPRHRHAAAVVGSKIYVFGGLNNEIIY 2308
            GGR  P KILNDVW F+T+KN WKLL+C GSVF PRHRHAAAV+GS IYVFGGL+N+II+
Sbjct: 352  GGRTGPDKILNDVWSFDTTKNCWKLLQCGGSVFPPRHRHAAAVMGSNIYVFGGLDNDIIF 411

Query: 2307 SSMYVLDSENSRWSEITTKGEQPCSRHSHALVANDSQLFMFGGYDGEKVFSDLYRFDTIA 2128
            SS ++LD+ N  W EI   G+ P +RHSHA+VA+DS++FMFGGYDG K   D+Y FD   
Sbjct: 412  SSFFILDTVNLHWKEIPVSGDWPYARHSHAMVASDSRIFMFGGYDGGKALGDMYSFDVQM 471

Query: 2127 CLWKKEKTAGTPPLAKFSHSMFIYKNYLGILGGCPVIQNNQEMSLLDLRIGLWKHVILNT 1948
              WKKE TAG  P  +FSHS+F+YKNYLG+LGGCPV Q+ QE++LLDL++ +WKHV LN+
Sbjct: 472  SQWKKEITAGRNPHPRFSHSIFVYKNYLGVLGGCPVTQHCQELALLDLKLHIWKHVTLNS 531

Query: 1947 VSKELFVRSTXXXXXXXXXXXXXGASCYAFGTKFSLPMKISLLPYVSFSDTVTLHSKEMP 1768
            V K+LFVRST             GASCYAFGTKFS P K+SLL       ++  H   MP
Sbjct: 532  VGKDLFVRSTANVVGDDLVIVGGGASCYAFGTKFSEPAKVSLL------HSMHSHDDFMP 585

Query: 1767 SRHHPETVLENITGSVFFTAKNVQTLTKSCISNYKTEVLGESFDIEQGAT-----HWVLR 1603
             ++  + +++   G+    ++  Q      IS  ++    E+     G +     H VL+
Sbjct: 586  VKNQKQHIIDQNGGNKVENSQGPQLEHPPNISENESLYFNENVLHINGQSQTIPLHCVLQ 645

Query: 1602 LERKYAKLGKDILKKFGWLDLRRKVYSSENGSHICLPVTEKFYPLFHKKQFNLMDSNDGL 1423
            LE+KYAK GKDILKKFGWLDL RKVYS E G HIC PV ++ + +FH++      S D +
Sbjct: 646  LEKKYAKQGKDILKKFGWLDLGRKVYSEEGGVHICFPVHQELFAVFHERS---QHSGDPI 702

Query: 1422 STPCKLVNIEELSTYDF-----TCEMALDNLFLLGGLIVKDEVACERIAPKSPQKVMSEA 1258
                K+   + L+   +     +C  AL  L   G ++++D+V   + A  SP KVMSE 
Sbjct: 703  DRENKIPLSKPLTQAGYLLNKLSCSEALTLLHEYGAVLLEDKVVETKKAAMSPLKVMSEG 762

Query: 1257 VESLIMKMGLPARLLEEVPTRWERLGDIVVLPVNSFRDASWNSIGQELWPTVAKSLGTRR 1078
            V SLI + GLP  LLEE+PTRW+R+GDIV+LP  SF+++ W+SI +ELW  VAKSL   R
Sbjct: 763  VTSLIEEKGLPTGLLEELPTRWDRIGDIVILPATSFKNSLWDSIAEELWLIVAKSLKAHR 822

Query: 1077 LARQGRVASTGTRDSTLDILVGDDGWVDHRENGIMYSFDATKCMFSWGNLSEKLRMACLD 898
            LARQG VA+TGTRDSTL+ILVGDDGWV+HRENGI YSF+ATKCMFSWGNLSEKLRMA +D
Sbjct: 823  LARQGPVAATGTRDSTLEILVGDDGWVNHRENGIHYSFNATKCMFSWGNLSEKLRMAQMD 882

Query: 897  CKGEVIVDLFAGIGYFVLPFLVRAKAKMVYACEWNPHALEALQRNVESNSVGDRCVILEG 718
            CK EVIVDLFAGIGYFVLPFLVRA AK+VYACEWNPHA+EAL+ N++SNSV +RC+++EG
Sbjct: 883  CKDEVIVDLFAGIGYFVLPFLVRAHAKLVYACEWNPHAIEALRHNLQSNSVAERCIVIEG 942

Query: 717  DNRATAPKGVADRVCLGLLPSSEGSWVTAVRALRMEGGLLHVHGIAKDSEESIWSDHVAN 538
            DNR TAPKGVADRVCLGLLPSSE SWVTAVRALR EGG+LHVHG AKDSEE  W+DHV+ 
Sbjct: 943  DNRNTAPKGVADRVCLGLLPSSECSWVTAVRALRKEGGILHVHGNAKDSEEYQWTDHVSK 1002

Query: 537  SIQSIAKSEGLCWEVSVQHLERVKWYGPHIRHLVLDVRCKQ 415
            SI  IA+SEG CWEV+++H+ERVKWY PHIRH+V DVRCKQ
Sbjct: 1003 SIYEIARSEGYCWEVTIEHVERVKWYAPHIRHVVADVRCKQ 1043