BLASTX nr result
ID: Coptis25_contig00005970
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00005970
(1309 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chl... 385 0.0
gb|ACF17660.1| putative isopropylmalate synthase [Capsicum annuum] 382 0.0
ref|XP_002530549.1| isopropylmalate synthase, putative [Ricinus ... 379 0.0
ref|XP_004165080.1| PREDICTED: 2-isopropylmalate synthase 2, chl... 378 e-180
ref|NP_177544.1| 2-isopropylmalate synthase 2 [Arabidopsis thali... 374 e-179
>ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Vitis
vinifera]
Length = 628
Score = 385 bits (990), Expect(3) = 0.0
Identities = 192/247 (77%), Positives = 219/247 (88%), Gaps = 1/247 (0%)
Frame = -3
Query: 869 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 690
ERAGNASLEEVVMA++CRGE+ LGGL+TGIN RHIV SKMVAE++GLQVQPHKAIVGAN
Sbjct: 324 ERAGNASLEEVVMALKCRGEKALGGLYTGINIRHIVKTSKMVAEYSGLQVQPHKAIVGAN 383
Query: 689 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 510
AFAHESGIHQDGMLK+K TYEI+SPEDIGL SN+SGIVLGKLSGRHAL+SRLLELGYE+
Sbjct: 384 AFAHESGIHQDGMLKHKETYEIISPEDIGLFRSNESGIVLGKLSGRHALKSRLLELGYEI 443
Query: 509 MA-ELDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 333
ELD +FWRFK VAEKKK+L+D D+ AL+SDEIFQP+ W LGDLQV CGTLGLSTAT
Sbjct: 444 EGMELDDIFWRFKEVAEKKKNLTDVDVIALVSDEIFQPKVFWKLGDLQVTCGTLGLSTAT 503
Query: 332 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 153
VKLI DG++H+ CSVGTGPVD+AY+AID +VKE VTLLEY +N+VTEGIDAIA+T VLI
Sbjct: 504 VKLIDADGQKHVACSVGTGPVDSAYKAIDLLVKEQVTLLEYGLNAVTEGIDAIATTRVLI 563
Query: 152 AGENGHS 132
GE H+
Sbjct: 564 RGEKNHT 570
Score = 256 bits (655), Expect(3) = 0.0
Identities = 128/148 (86%), Positives = 139/148 (93%)
Frame = -2
Query: 1308 VKYAKRPRIHTFIATSEIHLKYKLRKSREEVIAIAKEMVGYARSLGCNDVEFSPEDAGRS 1129
VKYAKRPRIHTFIATSEIH+KYKL+KS +EVI IA+EMV YARSLGC+DVEFSPEDAGRS
Sbjct: 177 VKYAKRPRIHTFIATSEIHMKYKLKKSEKEVIEIAREMVKYARSLGCDDVEFSPEDAGRS 236
Query: 1128 DREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFGKLIADIKLNTPGIDDVIISTHCQND 949
+REFLYKIL EVIKAGATTLNIPDTVGYT+PNEFG+LIADIK NTPGI++VIISTHCQND
Sbjct: 237 EREFLYKILGEVIKAGATTLNIPDTVGYTVPNEFGQLIADIKSNTPGIENVIISTHCQND 296
Query: 948 LGLSTANTIERVGAGARQLEVTINGIGE 865
LGLSTANTI AGARQ+EVTINGIGE
Sbjct: 297 LGLSTANTIAGACAGARQVEVTINGIGE 324
Score = 65.5 bits (158), Expect(3) = 0.0
Identities = 32/45 (71%), Positives = 37/45 (82%)
Frame = -1
Query: 136 THAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKMLDFSRTSK 2
T+A+TG+TVHR+FSG GAGMDIVVSS Y+SALNKML F SK
Sbjct: 572 TNALTGETVHRSFSGTGAGMDIVVSSVRAYISALNKMLGFKDQSK 616
>gb|ACF17660.1| putative isopropylmalate synthase [Capsicum annuum]
Length = 602
Score = 382 bits (981), Expect(3) = 0.0
Identities = 188/247 (76%), Positives = 224/247 (90%), Gaps = 1/247 (0%)
Frame = -3
Query: 869 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 690
ERAGNASLEEVVMA++CRGE++LGGL+TGINT+HI+M+SKMV E+TGL VQPHKAIVGAN
Sbjct: 299 ERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHILMSSKMVEEYTGLHVQPHKAIVGAN 358
Query: 689 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 510
AFAHESGIHQDGMLK+K TYEIMSPEDIGL+ +N+SGIVLGKLSGRHAL++++LELGYE+
Sbjct: 359 AFAHESGIHQDGMLKHKDTYEIMSPEDIGLNRANESGIVLGKLSGRHALQAKMLELGYEI 418
Query: 509 MA-ELDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 333
ELD +FWRFK+VAEKKK ++D D+ AL+SDE+FQPQ +W L ++QV CG+LGLSTAT
Sbjct: 419 EGKELDDLFWRFKSVAEKKKKITDDDLIALMSDEVFQPQFVWQLQNVQVTCGSLGLSTAT 478
Query: 332 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 153
VKLI DG+EHI CSVGTGPVDAAY+A+D IVK VTLLEYS+N+VT+GIDAIAST VLI
Sbjct: 479 VKLIDADGKEHISCSVGTGPVDAAYKAVDLIVKIPVTLLEYSMNAVTQGIDAIASTRVLI 538
Query: 152 AGENGHS 132
GENGH+
Sbjct: 539 RGENGHT 545
Score = 243 bits (621), Expect(3) = 0.0
Identities = 120/148 (81%), Positives = 133/148 (89%)
Frame = -2
Query: 1308 VKYAKRPRIHTFIATSEIHLKYKLRKSREEVIAIAKEMVGYARSLGCNDVEFSPEDAGRS 1129
VKYAK+PRIHTFIATSEIH+K+KL+ SR+EVI A+ MV YARS+GC DVEFSPEDAGRS
Sbjct: 152 VKYAKKPRIHTFIATSEIHMKFKLKMSRDEVIEKARSMVAYARSIGCQDVEFSPEDAGRS 211
Query: 1128 DREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFGKLIADIKLNTPGIDDVIISTHCQND 949
D EFLY IL EVIKAGATTLNIPDTVGYT+P EFG+LIA+IK NTPG++DVIISTHCQND
Sbjct: 212 DPEFLYHILGEVIKAGATTLNIPDTVGYTVPEEFGQLIANIKANTPGVEDVIISTHCQND 271
Query: 948 LGLSTANTIERVGAGARQLEVTINGIGE 865
LGLSTANT+ AGARQLEVTINGIGE
Sbjct: 272 LGLSTANTLAGACAGARQLEVTINGIGE 299
Score = 63.2 bits (152), Expect(3) = 0.0
Identities = 28/43 (65%), Positives = 35/43 (81%)
Frame = -1
Query: 139 ATHAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKMLDFSR 11
+THA+TG+TVHRTFSG GA MDIV+SS Y+ ALNKM+ F +
Sbjct: 546 STHALTGETVHRTFSGTGADMDIVISSVRAYIGALNKMMSFRK 588
>ref|XP_002530549.1| isopropylmalate synthase, putative [Ricinus communis]
gi|223529911|gb|EEF31840.1| isopropylmalate synthase,
putative [Ricinus communis]
Length = 632
Score = 379 bits (973), Expect(3) = 0.0
Identities = 189/247 (76%), Positives = 214/247 (86%), Gaps = 1/247 (0%)
Frame = -3
Query: 869 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 690
ERAGNASLEEVVMAI+CRGE +LGGL+TGIN R I MASKMV E+TGL +QPHKAIVGAN
Sbjct: 326 ERAGNASLEEVVMAIKCRGEHILGGLYTGINPRQITMASKMVEEYTGLHLQPHKAIVGAN 385
Query: 689 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 510
AFAHESGIHQDGMLK+KGTYEI+SPEDIGL SND+GIVLGKLSGRHAL+ RL ELGYEL
Sbjct: 386 AFAHESGIHQDGMLKHKGTYEIISPEDIGLERSNDAGIVLGKLSGRHALKDRLKELGYEL 445
Query: 509 MAE-LDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 333
E L +FWRFKAVAE KK ++D D+ AL+SDE+FQP+ +W LGDLQV CGTLGLSTAT
Sbjct: 446 NDEQLGTIFWRFKAVAETKKRVTDADLIALVSDEVFQPEIVWKLGDLQVTCGTLGLSTAT 505
Query: 332 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 153
VKLI DGEEHI CSVG+GPVDA Y+A+D IVKE V LLEYS+N+VTEGIDAIA+T VLI
Sbjct: 506 VKLINADGEEHIACSVGSGPVDAGYKAVDLIVKEPVELLEYSMNAVTEGIDAIATTRVLI 565
Query: 152 AGENGHS 132
E+ H+
Sbjct: 566 RREDNHT 572
Score = 248 bits (633), Expect(3) = 0.0
Identities = 124/148 (83%), Positives = 135/148 (91%)
Frame = -2
Query: 1308 VKYAKRPRIHTFIATSEIHLKYKLRKSREEVIAIAKEMVGYARSLGCNDVEFSPEDAGRS 1129
VKYAKRPRIHTFIATS IH++YKLRKS+EEV+ IA+ MV +ARSLGC+DVEFSPEDAGRS
Sbjct: 179 VKYAKRPRIHTFIATSAIHMEYKLRKSKEEVVEIARSMVKFARSLGCDDVEFSPEDAGRS 238
Query: 1128 DREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFGKLIADIKLNTPGIDDVIISTHCQND 949
DREFLY IL EVIKAGATTLNIPDTVG TLP+EFG+LIADIK NTPGID VIISTHCQND
Sbjct: 239 DREFLYHILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIDKVIISTHCQND 298
Query: 948 LGLSTANTIERVGAGARQLEVTINGIGE 865
LGLSTANT+ AGARQ+EVTINGIGE
Sbjct: 299 LGLSTANTLAGAYAGARQVEVTINGIGE 326
Score = 57.8 bits (138), Expect(3) = 0.0
Identities = 28/39 (71%), Positives = 32/39 (82%)
Frame = -1
Query: 139 ATHAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKML 23
+THA+TG+ RTFSG+GAGMDIVVSS YV ALNKML
Sbjct: 573 STHALTGEPFQRTFSGSGAGMDIVVSSVKAYVGALNKML 611
>ref|XP_004165080.1| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cucumis
sativus]
Length = 639
Score = 378 bits (970), Expect(3) = e-180
Identities = 187/247 (75%), Positives = 215/247 (87%), Gaps = 1/247 (0%)
Frame = -3
Query: 869 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 690
ERAGNASLEEVVMA++CRGE +LGGLHTGIN+RHI + SKMV EFTGL VQPHKAIVGAN
Sbjct: 331 ERAGNASLEEVVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGAN 390
Query: 689 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 510
AFAH SGIHQDGMLK+KGTYEIM+PEDIG SND+GIVLGKLSGRHAL+S LLELGYEL
Sbjct: 391 AFAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYEL 450
Query: 509 MAE-LDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 333
E LD VFWRFKAVAE+KK ++D D+ AL+SDE+FQP +W L D+QV CGTLGLSTAT
Sbjct: 451 DGENLDNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTAT 510
Query: 332 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 153
VKL+ DG+EHI CSVGTGPVD+AY+A+D IVKE TLLEYS+N+VTEGIDAIA+T VLI
Sbjct: 511 VKLLDADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLI 570
Query: 152 AGENGHS 132
G+ ++
Sbjct: 571 RGDKSYT 577
Score = 247 bits (631), Expect(3) = e-180
Identities = 122/148 (82%), Positives = 138/148 (93%)
Frame = -2
Query: 1308 VKYAKRPRIHTFIATSEIHLKYKLRKSREEVIAIAKEMVGYARSLGCNDVEFSPEDAGRS 1129
VKYAKRPRIHTFIATSEIH+++KLRK++EEVI IA+ MV +ARSLGC+DVEFSPEDAGRS
Sbjct: 184 VKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDAGRS 243
Query: 1128 DREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFGKLIADIKLNTPGIDDVIISTHCQND 949
DREFLY+IL EVIKAGATTLNIPDTVGYT+P EFGKLIADIK NTPGI++VIIS+HCQND
Sbjct: 244 DREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHCQND 303
Query: 948 LGLSTANTIERVGAGARQLEVTINGIGE 865
LGL+TANT+ AGARQ+EVTINGIGE
Sbjct: 304 LGLATANTVAGACAGARQVEVTINGIGE 331
Score = 58.2 bits (139), Expect(3) = e-180
Identities = 28/41 (68%), Positives = 33/41 (80%)
Frame = -1
Query: 139 ATHAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKMLDF 17
+T+A+TG+ V RTFSG GAGMDIVVSS Y+ ALNKML F
Sbjct: 578 STNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 618
>ref|NP_177544.1| 2-isopropylmalate synthase 2 [Arabidopsis thaliana]
gi|75168891|sp|Q9C550.1|LEU12_ARATH RecName:
Full=2-isopropylmalate synthase 2, chloroplastic;
AltName: Full=2-isopropylmalate synthase 1; AltName:
Full=Methylthioalkylmalate synthase-like 3; Flags:
Precursor gi|12325154|gb|AAG52530.1|AC016662_24 putative
2-isopropylmalate synthase; 30920-27612 [Arabidopsis
thaliana] gi|12330687|gb|AAG52882.1|AF327647_1
2-isopropylmalate synthase [Arabidopsis thaliana]
gi|16323111|gb|AAL15290.1| At1g74040/F2P9_9 [Arabidopsis
thaliana] gi|23463041|gb|AAN33190.1| At1g74040/F2P9_9
[Arabidopsis thaliana] gi|332197419|gb|AEE35540.1|
2-isopropylmalate synthase 2 [Arabidopsis thaliana]
Length = 631
Score = 374 bits (959), Expect(3) = e-179
Identities = 184/247 (74%), Positives = 216/247 (87%), Gaps = 1/247 (0%)
Frame = -3
Query: 869 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 690
ERAGNASLEEVVMAI+CRG+ +LGGL TGI+TRHIVM SKMV E+TG+Q QPHKAIVGAN
Sbjct: 325 ERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGAN 384
Query: 689 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 510
AFAHESGIHQDGMLK+KGTYEIMSPE+IGL SND+GIVLGKLSGRHAL+ RL ELGY L
Sbjct: 385 AFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVL 444
Query: 509 -MAELDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 333
+L +FWRFKAVAE+KK ++D D+ AL+SDE+FQP+A+W L D+Q+ CGTLGLST+T
Sbjct: 445 DDGQLSNLFWRFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTST 504
Query: 332 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 153
VKL DG+EH+ CSVGTGPVDAAY+A+D IVKE TLLEYS+N+VTEGIDAIA+T VLI
Sbjct: 505 VKLADSDGKEHVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLI 564
Query: 152 AGENGHS 132
G+N +S
Sbjct: 565 RGDNNYS 571
Score = 246 bits (629), Expect(3) = e-179
Identities = 122/148 (82%), Positives = 136/148 (91%)
Frame = -2
Query: 1308 VKYAKRPRIHTFIATSEIHLKYKLRKSREEVIAIAKEMVGYARSLGCNDVEFSPEDAGRS 1129
VKYAKRPRIHTFIATS+IHLKYKL+KS+EEVI IA+ MV +ARSLGC DVEFSPEDAGRS
Sbjct: 178 VKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRS 237
Query: 1128 DREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFGKLIADIKLNTPGIDDVIISTHCQND 949
+RE+LY+IL EVIKAGATTLNIPDTVG TLP+EFG+LIADIK NTPGI +VIISTHCQND
Sbjct: 238 EREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQND 297
Query: 948 LGLSTANTIERVGAGARQLEVTINGIGE 865
LGLSTANT+ +GARQ+EVTINGIGE
Sbjct: 298 LGLSTANTLSGAHSGARQVEVTINGIGE 325
Score = 59.7 bits (143), Expect(3) = e-179
Identities = 29/41 (70%), Positives = 34/41 (82%)
Frame = -1
Query: 139 ATHAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKMLDF 17
+T+A+TG++V RTFSG GAGMDIVVSS YV ALNKML F
Sbjct: 572 STNAVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGF 612