BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00005888
         (1635 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa] g...   358   2e-96
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop...   346   9e-93
emb|CBI24150.3| unnamed protein product [Vitis vinifera]              346   9e-93
dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa]             338   3e-90
sp|Q39548.1|KSB_CUCMA RecName: Full=Ent-kaur-16-ene synthase, ch...   337   7e-90

>ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa]
           gi|222851106|gb|EEE88653.1| ent-kaurene synthase
           [Populus trichocarpa]
          Length = 743

 Score =  358 bits (920), Expect = 2e-96
 Identities = 170/265 (64%), Positives = 206/265 (77%)
 Frame = -2

Query: 797 GPKEKIREMFNKIELSVSSYDTAWVAMVPSPDSSQQPCFPECLNWLLENQVRDGSWTLPH 618
           G KE+I++MF+KIELSVSSYDTAWVAMVPSPD  + PCFPEC  W+LENQ+ DGSW+LPH
Sbjct: 6   GTKERIKKMFDKIELSVSSYDTAWVAMVPSPDCPETPCFPECTKWILENQLGDGSWSLPH 65

Query: 617 HHHLLIKESMLSTLACVLALRRWETGDEHVRKGLEFMELNFGLIMDKKHTSPIGFDIIFP 438
            + LL+K+++ STLAC+LAL+RW  G+E + KGL F+ELN   + D +   PIGFDIIFP
Sbjct: 66  GNPLLVKDALSSTLACILALKRWGIGEEQINKGLRFIELNSASVTDNEQHKPIGFDIIFP 125

Query: 437 GMIEYAKDLDLNLPFSPKFVEAMLHKRDLEFERVSKINSKGSKAYLAYTSEGLTKLCDWK 258
           GMIEYA DLDLNLP  P  + +MLH+R LE       N +G +AYLAY SEG+ KL DW+
Sbjct: 126 GMIEYAIDLDLNLPLKPTDINSMLHRRALELTSGGGKNLEGRRAYLAYVSEGIGKLQDWE 185

Query: 257 EVMKWQRKNGSMFNSPSTTAATFIQNQDAKCLEYLRLLLKTFANAVPTSYPVDVYIHLSM 78
             MK+QRKNGS+FNSPSTTAA FI  QDA+CL Y+R LL+ F NAVPT YP+D+Y  LSM
Sbjct: 186 MAMKYQRKNGSLFNSPSTTAAAFIHIQDAECLHYIRSLLQKFGNAVPTIYPLDIYARLSM 245

Query: 77  VDRLERMGIGRHFRNEIRSVLDETY 3
           VD LER+GI RHFR E + VLDETY
Sbjct: 246 VDALERLGIDRHFRKERKFVLDETY 270


>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
           vinifera]
          Length = 793

 Score =  346 bits (888), Expect = 9e-93
 Identities = 177/313 (56%), Positives = 221/313 (70%), Gaps = 3/313 (0%)
 Frame = -2

Query: 932 LCFFSSNLMFL---SRPKTRMISSRHDGDAFRRLHVVRRAENAINCAQGPKEKIREMFNK 762
           +  +SSN + L    +PK+  +++         L       +     +G KE+I++MF+K
Sbjct: 8   MALYSSNSLLLLLHHKPKSSCVTAPISASLSPGLKTWTEGNHVGLGFEGTKERIKKMFDK 67

Query: 761 IELSVSSYDTAWVAMVPSPDSSQQPCFPECLNWLLENQVRDGSWTLPHHHHLLIKESMLS 582
           +ELSVSSYDTAWVAMVPSP SSQ P FPEC+NWLLENQ  DGSW LPH H +L+K+++ S
Sbjct: 68  VELSVSSYDTAWVAMVPSPYSSQAPYFPECVNWLLENQSHDGSWGLPHPHPMLVKDALSS 127

Query: 581 TLACVLALRRWETGDEHVRKGLEFMELNFGLIMDKKHTSPIGFDIIFPGMIEYAKDLDLN 402
           TLA VLAL+RW  G+E   KGL F+  NF  + D+K  SPIGFDIIFPGMIEYAK+LDLN
Sbjct: 128 TLASVLALKRWGVGEEQRNKGLWFIASNFASVSDEKQHSPIGFDIIFPGMIEYAKELDLN 187

Query: 401 LPFSPKFVEAMLHKRDLEFERVSKINSKGSKAYLAYTSEGLTKLCDWKEVMKWQRKNGSM 222
           LP   + V+AML KRDLE +     N+K  +AYLAY SEG+ +L DW+ VMK+Q KNGS+
Sbjct: 188 LPLGQRDVDAMLQKRDLELKGSLGSNTKSREAYLAYISEGMGRLQDWEMVMKYQMKNGSL 247

Query: 221 FNSPSTTAATFIQNQDAKCLEYLRLLLKTFANAVPTSYPVDVYIHLSMVDRLERMGIGRH 42
            NSPS TAA     Q+A CL YLR LL+ F NAVPT YP+D+Y  L +VD LER+GI R+
Sbjct: 248 LNSPSATAAALSHLQNAGCLNYLRSLLEKFGNAVPTVYPLDLYARLCLVDNLERLGIDRY 307

Query: 41  FRNEIRSVLDETY 3
           FR EIRSVLDETY
Sbjct: 308 FRMEIRSVLDETY 320


>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
          Length = 826

 Score =  346 bits (888), Expect = 9e-93
 Identities = 177/313 (56%), Positives = 221/313 (70%), Gaps = 3/313 (0%)
 Frame = -2

Query: 932 LCFFSSNLMFL---SRPKTRMISSRHDGDAFRRLHVVRRAENAINCAQGPKEKIREMFNK 762
           +  +SSN + L    +PK+  +++         L       +     +G KE+I++MF+K
Sbjct: 33  MALYSSNSLLLLLHHKPKSSCVTAPISASLSPGLKTWTEGNHVGLGFEGTKERIKKMFDK 92

Query: 761 IELSVSSYDTAWVAMVPSPDSSQQPCFPECLNWLLENQVRDGSWTLPHHHHLLIKESMLS 582
           +ELSVSSYDTAWVAMVPSP SSQ P FPEC+NWLLENQ  DGSW LPH H +L+K+++ S
Sbjct: 93  VELSVSSYDTAWVAMVPSPYSSQAPYFPECVNWLLENQSHDGSWGLPHPHPMLVKDALSS 152

Query: 581 TLACVLALRRWETGDEHVRKGLEFMELNFGLIMDKKHTSPIGFDIIFPGMIEYAKDLDLN 402
           TLA VLAL+RW  G+E   KGL F+  NF  + D+K  SPIGFDIIFPGMIEYAK+LDLN
Sbjct: 153 TLASVLALKRWGVGEEQRNKGLWFIASNFASVSDEKQHSPIGFDIIFPGMIEYAKELDLN 212

Query: 401 LPFSPKFVEAMLHKRDLEFERVSKINSKGSKAYLAYTSEGLTKLCDWKEVMKWQRKNGSM 222
           LP   + V+AML KRDLE +     N+K  +AYLAY SEG+ +L DW+ VMK+Q KNGS+
Sbjct: 213 LPLGQRDVDAMLQKRDLELKGSLGSNTKSREAYLAYISEGMGRLQDWEMVMKYQMKNGSL 272

Query: 221 FNSPSTTAATFIQNQDAKCLEYLRLLLKTFANAVPTSYPVDVYIHLSMVDRLERMGIGRH 42
            NSPS TAA     Q+A CL YLR LL+ F NAVPT YP+D+Y  L +VD LER+GI R+
Sbjct: 273 LNSPSATAAALSHLQNAGCLNYLRSLLEKFGNAVPTVYPLDLYARLCLVDNLERLGIDRY 332

Query: 41  FRNEIRSVLDETY 3
           FR EIRSVLDETY
Sbjct: 333 FRMEIRSVLDETY 345


>dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa]
          Length = 788

 Score =  338 bits (866), Expect = 3e-90
 Identities = 161/263 (61%), Positives = 199/263 (75%)
 Frame = -2

Query: 791 KEKIREMFNKIELSVSSYDTAWVAMVPSPDSSQQPCFPECLNWLLENQVRDGSWTLPHHH 612
           KE+IR++FN +E+SVSSYDTAWVAMVPSP+S + PCFP+CLNWLL+NQ+ DGSW L  H 
Sbjct: 58  KERIRKLFNNVEVSVSSYDTAWVAMVPSPNSPKSPCFPDCLNWLLDNQLDDGSWGLLPHQ 117

Query: 611 HLLIKESMLSTLACVLALRRWETGDEHVRKGLEFMELNFGLIMDKKHTSPIGFDIIFPGM 432
             LIK+++ STLACVLAL+RW  G + + KGL ++E NF  + DK   SP GFDIIFPGM
Sbjct: 118 SPLIKDTLSSTLACVLALKRWNVGKDQINKGLHYIESNFASVTDKNQASPFGFDIIFPGM 177

Query: 431 IEYAKDLDLNLPFSPKFVEAMLHKRDLEFERVSKINSKGSKAYLAYTSEGLTKLCDWKEV 252
           +EYAKDLD+ LP +   +  MLH+R+LE  R    +S G +AYLAY SEGL  L DW  V
Sbjct: 178 LEYAKDLDIKLPLNQTHLSVMLHERELELRR---CHSNGREAYLAYISEGLGNLNDWNMV 234

Query: 251 MKWQRKNGSMFNSPSTTAATFIQNQDAKCLEYLRLLLKTFANAVPTSYPVDVYIHLSMVD 72
           MK+Q KNGS+FNSPS TA+  I +Q+A CL YL  LL  F NAVPT YP+D+Y+ LSMVD
Sbjct: 235 MKYQMKNGSLFNSPSATASVLIHHQNAGCLHYLTSLLDKFGNAVPTVYPIDLYVRLSMVD 294

Query: 71  RLERMGIGRHFRNEIRSVLDETY 3
            LER+GI RHF  EI++VLDETY
Sbjct: 295 TLERLGIKRHFMVEIQNVLDETY 317


>sp|Q39548.1|KSB_CUCMA RecName: Full=Ent-kaur-16-ene synthase, chloroplastic; AltName:
           Full=Ent-kaurene synthase; AltName: Full=Ent-kaurene
           synthase B; Short=KSB; Flags: Precursor
           gi|1431870|gb|AAB39482.1| ent-kaurene synthase B
           [Cucurbita maxima]
          Length = 789

 Score =  337 bits (863), Expect = 7e-90
 Identities = 167/307 (54%), Positives = 217/307 (70%), Gaps = 5/307 (1%)
 Frame = -2

Query: 908 MFLSRPK-----TRMISSRHDGDAFRRLHVVRRAENAINCAQGPKEKIREMFNKIELSVS 744
           M+LSRP          SS      F  + V    +      +  KE+I+++F+K+ELSVS
Sbjct: 1   MYLSRPTGVARFAASSSSSSSASLFPGVDVDTTTKTGALHFEETKERIKKLFDKVELSVS 60

Query: 743 SYDTAWVAMVPSPDSSQQPCFPECLNWLLENQVRDGSWTLPHHHHLLIKESMLSTLACVL 564
           +YDTAWVAMVPSP+S  QP FPEC+NW+L++Q  DGSW L H+  LL+K ++LSTLACVL
Sbjct: 61  AYDTAWVAMVPSPNSLNQPLFPECINWVLDSQHADGSWGLLHNDQLLMKANLLSTLACVL 120

Query: 563 ALRRWETGDEHVRKGLEFMELNFGLIMDKKHTSPIGFDIIFPGMIEYAKDLDLNLPFSPK 384
            L+RW  G +H+ K L+F++ N     D+   SP+GFDIIFPGMIEYAKDL+LNLP +P 
Sbjct: 121 TLKRWNIGHDHMSKALDFIKSNIASATDENQRSPVGFDIIFPGMIEYAKDLNLNLPLAPT 180

Query: 383 FVEAMLHKRDLEFERVSKINSKGSKAYLAYTSEGLTKLCDWKEVMKWQRKNGSMFNSPST 204
            V+A++ K++LE  R  + NS+G KAYLAY SEG+ KL DW  VM++QRKNGS+FNSPST
Sbjct: 181 NVDALVRKKELEL-RSCRSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGSLFNSPST 239

Query: 203 TAATFIQNQDAKCLEYLRLLLKTFANAVPTSYPVDVYIHLSMVDRLERMGIGRHFRNEIR 24
           TAA F+   D  C +YLR LL+ F  +VPT YP+D+Y  L MVD L++ GI RHF+ EIR
Sbjct: 240 TAAAFMHRNDDGCFDYLRSLLQKFDGSVPTIYPLDIYARLHMVDSLQKFGIARHFKEEIR 299

Query: 23  SVLDETY 3
           SVLDETY
Sbjct: 300 SVLDETY 306