BLASTX nr result
ID: Coptis25_contig00005728
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00005728
(1849 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004144122.1| PREDICTED: mechanosensitive ion channel prot... 108 3e-35
ref|XP_004170731.1| PREDICTED: mechanosensitive ion channel prot... 108 3e-35
ref|XP_002309474.1| predicted protein [Populus trichocarpa] gi|2... 105 5e-34
ref|XP_002278293.1| PREDICTED: uncharacterized protein At5g12080... 106 9e-34
ref|XP_003523652.1| PREDICTED: uncharacterized protein At5g12080... 107 3e-33
>ref|XP_004144122.1| PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis
sativus]
Length = 762
Score = 108 bits (269), Expect(2) = 3e-35
Identities = 66/170 (38%), Positives = 88/170 (51%)
Frame = +2
Query: 329 PSPEISKFSPSPNRPPKHPESTLTRRHSLARSAFEKPKSRFVEPFSPXXXXXXXXXXXXX 508
PS EIS+ +P ++PPK P T+TRR S A S+F KPKSR +EP P
Sbjct: 60 PSHEISRMTP--HKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAK 117
Query: 509 XXPYKLSPNNNNSKKVSFSEHGKDGLRNIPITPKTPLMGXXXXXXXXXXXXXXXXXSVAT 688
Y S ++ K++ K+ L+ PITPKTPL+G +
Sbjct: 118 STLYS-SSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRT--AELK 174
Query: 689 KMEKSRKKLKVFALIEWVALFCILGGLLASLTVCRLEHFMIWGLQLWKWC 838
EKS K+LK ++EW+A C+ G L+ASLT+ L IWGL LWKWC
Sbjct: 175 VKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWC 224
Score = 68.9 bits (167), Expect(2) = 3e-35
Identities = 30/44 (68%), Positives = 38/44 (86%)
Frame = +1
Query: 832 MVCVSVCGRLMTKWFVHVLVFLIEKNFLLKNKVLYFIYGVRKSV 963
+V V CGRL ++WF++ LVFLIE+NFLLK KVLYF+YG+RKSV
Sbjct: 226 LVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSV 269
>ref|XP_004170731.1| PREDICTED: mechanosensitive ion channel protein 10-like, partial
[Cucumis sativus]
Length = 420
Score = 108 bits (269), Expect(2) = 3e-35
Identities = 66/170 (38%), Positives = 88/170 (51%)
Frame = +2
Query: 329 PSPEISKFSPSPNRPPKHPESTLTRRHSLARSAFEKPKSRFVEPFSPXXXXXXXXXXXXX 508
PS EIS+ +P ++PPK P T+TRR S A S+F KPKSR +EP P
Sbjct: 60 PSHEISRMTP--HKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAK 117
Query: 509 XXPYKLSPNNNNSKKVSFSEHGKDGLRNIPITPKTPLMGXXXXXXXXXXXXXXXXXSVAT 688
Y S ++ K++ K+ L+ PITPKTPL+G +
Sbjct: 118 STLYS-SSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRT--AELK 174
Query: 689 KMEKSRKKLKVFALIEWVALFCILGGLLASLTVCRLEHFMIWGLQLWKWC 838
EKS K+LK ++EW+A C+ G L+ASLT+ L IWGL LWKWC
Sbjct: 175 VKEKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWC 224
Score = 68.9 bits (167), Expect(2) = 3e-35
Identities = 30/44 (68%), Positives = 38/44 (86%)
Frame = +1
Query: 832 MVCVSVCGRLMTKWFVHVLVFLIEKNFLLKNKVLYFIYGVRKSV 963
+V V CGRL ++WF++ LVFLIE+NFLLK KVLYF+YG+RKSV
Sbjct: 226 LVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSV 269
>ref|XP_002309474.1| predicted protein [Populus trichocarpa] gi|222855450|gb|EEE92997.1|
predicted protein [Populus trichocarpa]
Length = 622
Score = 105 bits (261), Expect(2) = 5e-34
Identities = 68/174 (39%), Positives = 86/174 (49%), Gaps = 3/174 (1%)
Frame = +2
Query: 326 CPSPEISKFSPSPNRPPK--HPESTLTRRHSLARSAFEKPKSRFVE-PFSPXXXXXXXXX 496
CPSPEIS S + +PPK P LTRR SL RS + KPKSRF E P+
Sbjct: 7 CPSPEISWSSQTQRKPPKIPTPNENLTRRRSLVRSVYSKPKSRFGEQPYGIDSTLLEENV 66
Query: 497 XXXXXXPYKLSPNNNNSKKVSFSEHGKDGLRNIPITPKTPLMGXXXXXXXXXXXXXXXXX 676
SP+ N + S + + ITPKTPLM
Sbjct: 67 STLQEQIATASPHRNLLTRGSPNNKSASSV----ITPKTPLMASPGGPADEEDEEEVWKR 122
Query: 677 SVATKMEKSRKKLKVFALIEWVALFCILGGLLASLTVCRLEHFMIWGLQLWKWC 838
++K +K R+++ A+IEWVA CILG L+ASLTV +LE IW L+ WKWC
Sbjct: 123 VESSK-QKQRRRVGAKAVIEWVAFLCILGCLIASLTVEKLEKTTIWSLEFWKWC 175
Score = 67.8 bits (164), Expect(2) = 5e-34
Identities = 30/46 (65%), Positives = 39/46 (84%)
Frame = +1
Query: 832 MVCVSVCGRLMTKWFVHVLVFLIEKNFLLKNKVLYFIYGVRKSVQV 969
+V V G L+T WF+HV+VFLIE+NFLLK KVLYF++G++KSVQV
Sbjct: 177 LVMVIFSGMLVTNWFMHVIVFLIERNFLLKKKVLYFVHGLKKSVQV 222
>ref|XP_002278293.1| PREDICTED: uncharacterized protein At5g12080-like [Vitis vinifera]
Length = 772
Score = 106 bits (264), Expect(2) = 9e-34
Identities = 62/174 (35%), Positives = 93/174 (53%), Gaps = 6/174 (3%)
Frame = +2
Query: 332 SPEISKFSPSPNRPPKHPESTLTRRHSLARSAFEKPKSRFVEPFSPXXXXXXXXXXXXXX 511
SP+I++ SP+ ++PPK P ++ RR SL SA+ KPKSR +EP P
Sbjct: 71 SPDIAR-SPNASKPPKVPTESVVRRRSLGSSAYSKPKSRLLEPSYPIETSVGEKT----- 124
Query: 512 XPYKLSPNNNNSKKVSFSEHG------KDGLRNIPITPKTPLMGXXXXXXXXXXXXXXXX 673
+L P+N+ + H +D +R P TP+TPL+
Sbjct: 125 ---QLLPSNSPIADSASPVHSLTATTPRDNVRTAPATPRTPLV---LDGEDEEEDDDVYK 178
Query: 674 XSVATKMEKSRKKLKVFALIEWVALFCILGGLLASLTVCRLEHFMIWGLQLWKW 835
S + ++EK+ K+L+ +EW+A CI+G L+ASLTV RL H +IWGL++WKW
Sbjct: 179 TSNSPEIEKNSKRLRFVLWVEWIAFVCIMGCLIASLTVHRLLHTLIWGLEIWKW 232
Score = 65.9 bits (159), Expect(2) = 9e-34
Identities = 27/46 (58%), Positives = 40/46 (86%)
Frame = +1
Query: 832 MVCVSVCGRLMTKWFVHVLVFLIEKNFLLKNKVLYFIYGVRKSVQV 969
+V V CGRL+T+W ++++VF+IE+NFLL+ KVLYF+YG++KSV V
Sbjct: 235 LVLVIFCGRLVTEWCINIVVFMIERNFLLRKKVLYFVYGLKKSVLV 280
>ref|XP_003523652.1| PREDICTED: uncharacterized protein At5g12080-like [Glycine max]
Length = 845
Score = 107 bits (267), Expect(2) = 3e-33
Identities = 74/190 (38%), Positives = 85/190 (44%), Gaps = 15/190 (7%)
Frame = +2
Query: 314 PSKFCPSPEISKFSPSPNRPPKHPEST--LTRRHSLARSAFEKPKSRFVEPFSPXXXXXX 487
P C SPEI +F PSPN+PPK P S LTRR SL RS + KPKSRF E P
Sbjct: 134 PPLNCASPEI-RFMPSPNKPPKVPTSNAILTRRKSLTRSVYSKPKSRFGEQSYPIDGTLL 192
Query: 488 XXXXXXXXX-------PYKLSPNNNNSKKVSFSEHGKDGLRNIP------ITPKTPLMGX 628
PYK SPNNNN K G N +TPKTPLM
Sbjct: 193 EENATSTLQENLTVGSPYKASPNNNN----------KPGTVNRTFSILSVVTPKTPLMAS 242
Query: 629 XXXXXXXXXXXXXXXXSVATKMEKSRKKLKVFALIEWVALFCILGGLLASLTVCRLEHFM 808
++ M R +KV L EW CI L+ASLTV +L+
Sbjct: 243 PGLAGEDFDEIIYKKVELSKNMRSRRLTVKV--LFEWFVFVCIASSLVASLTVGKLKRTE 300
Query: 809 IWGLQLWKWC 838
IWGL W+WC
Sbjct: 301 IWGLGFWRWC 310
Score = 62.8 bits (151), Expect(2) = 3e-33
Identities = 26/45 (57%), Positives = 38/45 (84%)
Frame = +1
Query: 832 MVCVSVCGRLMTKWFVHVLVFLIEKNFLLKNKVLYFIYGVRKSVQ 966
+V V+ CG L+T+WF+ ++VFLIE NFLL+ KVLYF++G++K VQ
Sbjct: 312 LVMVTFCGMLVTRWFMLIVVFLIETNFLLRKKVLYFVHGLKKCVQ 356