BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00005503
         (5116 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265386.1| PREDICTED: DNA topoisomerase 1-like [Vitis v...  1066   0.0  
dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]              1056   0.0  
dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]                1025   0.0  
emb|CBI28783.3| unnamed protein product [Vitis vinifera]             1008   0.0  
ref|XP_004149585.1| PREDICTED: DNA topoisomerase 1-like [Cucumis...   993   0.0  

>ref|XP_002265386.1| PREDICTED: DNA topoisomerase 1-like [Vitis vinifera]
          Length = 883

 Score = 1066 bits (2758), Expect = 0.0
 Identities = 568/887 (64%), Positives = 637/887 (71%), Gaps = 17/887 (1%)
 Frame = -1

Query: 2893 PVVFKRSKTTTKQNQLSAEVNRK-----SSVKSDAPSRKQVSDALSPNGQKSTIQQGKEG 2729
            PV    S+ +     LSA+++ +     S V     S    S     + +KS      E 
Sbjct: 10   PVANGESEDSEDDKPLSAKLSARLPKGNSHVLKGLSSPVLQSQKTKVDVKKSDGDSEDEV 69

Query: 2728 SSSKATAAKTHVTSSNDSTLVADKSIHLGTTLGNNNLEKKYLNKRHAEPT----SDSEDS 2561
              S     KT+V SS+            G +LG+  L    L+    E        S+DS
Sbjct: 70   PLSSKFPLKTYVRSSS------------GKSLGSKGLNSPSLSSHPPETKVGVKESSDDS 117

Query: 2560 DDKMPLSFRLPAGSAAPPKSNSPSVLKGLTXXXXXXXXSEKPKVSVK--SEDSEDDMPLS 2387
            +D++PL  +L   S     S+     KGL+        S+K KV+ K  S+DSED++PLS
Sbjct: 118  EDEIPLLLKLQLKSRKGASSSKLLDSKGLSSPASSRPQSQKAKVNNKNLSDDSEDEIPLS 177

Query: 2386 SKFHLKSGAGASVKKPYDSDEDKPLSSKIQQNGNVKRDNESSQTPKNSNKRPLGDANTSA 2207
            SKF LK+G GAS+ K  + DE KPL+S ++ NG  K+D+E  ++PK  NKRPLG ANTS 
Sbjct: 178  SKFRLKTGMGASLDKLDNFDEKKPLTSTLKPNGISKKDDERVKSPKGQNKRPLGQANTSH 237

Query: 2206 QXXXXXXXXXXXXXXXXXXXXXXKAEPXXXXXXXD------VPIAQRMTKSVTSDSKSKP 2045
                                   KAE        +       P  +R+ KSV+SD+K K 
Sbjct: 238  HSSSKKPKLLDGSVKGKVKQVSVKAEQKDDDDDDNDDDDEYTPTVKRLKKSVSSDNK-KA 296

Query: 2044 MKQTPXXXXXXXXXXXXXXXXXXXXXXKYLKSTKTPPGSGEGQKWTTLVHNGVIFPPPYK 1865
             K                         KY KSTK PP SGEGQKWTTL HNGVIFPPPYK
Sbjct: 297  SKTKVIPSSRKKATKKAKKSKKLAKSSKYSKSTKVPPNSGEGQKWTTLEHNGVIFPPPYK 356

Query: 1864 PHGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKFLENFMTDWRTVLGKNHIIKK 1685
            PHGVKM+YNG+PVDLTPEQEEVATMFAVMKDTDYATKP+F+ENFM DWR  LGKNH+IKK
Sbjct: 357  PHGVKMLYNGRPVDLTPEQEEVATMFAVMKDTDYATKPRFIENFMNDWRVRLGKNHVIKK 416

Query: 1684 FELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXXKYMWAIVDGVKEKVGNFRVE 1505
            FELCDFTPIYEWH+ EKEKKKQM+T               KYMWA+VDGVKEKVGNFRVE
Sbjct: 417  FELCDFTPIYEWHEKEKEKKKQMTTEEKKALKEEKLKQEDKYMWAVVDGVKEKVGNFRVE 476

Query: 1504 PPGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECSIPGERWKEVKHDNTVTWLAF 1325
            PPGLFRGRGEHPKMGKLKKRI P DITINIGK APVPEC IPGERWKEV+HDNTVTWLAF
Sbjct: 477  PPGLFRGRGEHPKMGKLKKRICPSDITINIGKEAPVPECPIPGERWKEVRHDNTVTWLAF 536

Query: 1324 WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYIENIRVNYAKDFTNKDVVKRQ 1145
            WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLK+YI+NIR  Y KDFTNKD +KRQ
Sbjct: 537  WNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKDYIQNIRATYTKDFTNKDTMKRQ 596

Query: 1144 VAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLVPPNKLEFDFLGKDSVRYFNT 965
            +AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV LVPPNKLEFDFLGKDS+RYFNT
Sbjct: 597  IAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVALVPPNKLEFDFLGKDSIRYFNT 656

Query: 964  VDVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTFNASITLDD 785
            V+VE+PVYKAIGQFK+GK  GDDLFDKLDTSKLNAHLKELMPGLTAKVFRT+NASITLD+
Sbjct: 657  VEVELPVYKAIGQFKTGKSGGDDLFDKLDTSKLNAHLKELMPGLTAKVFRTYNASITLDE 716

Query: 784  MLNKETRGGDVAQKIVVYQHANKEVAIICNHQRSVSKSHSAQMDKLTEKMNXXXXXXXXX 605
            MLN+E RGGD  +KIV+YQHANKEVAIICNHQRSVSKSH  QM +L+EKM          
Sbjct: 717  MLNREARGGDAMEKIVIYQHANKEVAIICNHQRSVSKSHDVQMSRLSEKMRELQGVLDEL 776

Query: 604  XXXXARARKGKPPLKDADGKTKKNLTPEALERKIAQTNTKIEKMERDRQTKEDLKTVALG 425
                ARA+KGKPPLKD+DGK KKN+TPE LERKIAQTNTKIEKMERD +TKEDLKTVALG
Sbjct: 777  KTDLARAKKGKPPLKDSDGKPKKNMTPEVLERKIAQTNTKIEKMERDMKTKEDLKTVALG 836

Query: 424  TSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMDVEPDFRF 284
            TSKINYLDPRISVAWCKR EVPIEKIFNKSLLAKFAWAMDV+P FRF
Sbjct: 837  TSKINYLDPRISVAWCKRQEVPIEKIFNKSLLAKFAWAMDVDPSFRF 883


>dbj|BAG31376.1| topoisomerase I [Camptotheca acuminata]
          Length = 894

 Score = 1056 bits (2731), Expect = 0.0
 Identities = 562/907 (61%), Positives = 653/907 (71%), Gaps = 17/907 (1%)
 Frame = -1

Query: 2953 MAVDACVKHKFMXXXXXXDAPVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPSRKQVSDAL 2774
            MAV ACVK K M      D P+VFKRS  T+K NQL+++  +KSS + D  S +QVSD  
Sbjct: 1    MAVQACVKEKMMDEIDEDDEPLVFKRSNATSKHNQLNSDT-KKSSQRYDGQSGRQVSDVP 59

Query: 2773 SPNGQKSTIQQGKEGSSSKATAAKTHVTSSNDSTLVA---------------DKSIHLGT 2639
            S NGQ S+  +GK   ++K ++ K+ + S   ST  A               D+S H   
Sbjct: 60   STNGQSSSASKGK---TTKLSSMKSPIASPKASTSFAKASPVANPKVSSSSDDRSKHSSK 116

Query: 2638 TLGNNNL-EKKYLNKRHAEPTSDSEDSDDKMPLSFRLPAGSAAPPKSNSPSVLKGLTXXX 2462
                N + E+K L    AEP  DSED   + PLS RL  G     K +S +  KGL    
Sbjct: 117  QNTINVVKEEKELVNPAAEPYGDSED---EKPLSARLFTGLT---KGSSNNANKGLINSS 170

Query: 2461 XXXXXSEKPKVSVKSEDSEDDMPLSSKFHLKSGAGASVKKPYDSDEDKPLSSKIQQNGNV 2282
                   KP+++  S+DS+D++PLSSKF LK+ AG S  K Y+SD++K L S  QQNG++
Sbjct: 171  PASLPVPKPEINRYSDDSDDEIPLSSKFRLKANAGTSTVKSYESDDNKTLVSNFQQNGSI 230

Query: 2281 KRDNESSQTPKNSNKRPLGDANTSAQXXXXXXXXXXXXXXXXXXXXXXKAEPXXXXXXXD 2102
             R ++SS      NKRPLG+  +S Q                      KAEP       D
Sbjct: 231  NRGSKSSI---KVNKRPLGEVKSSVQSSVKKPKLSDASTPVNNKQASKKAEPKADDSDDD 287

Query: 2101 -VPIAQRMTKSVTSDSKSKPMKQTPXXXXXXXXXXXXXXXXXXXXXXKYLKSTKTPPGSG 1925
             VPI+QRM KS TS +KS   KQ                        KY KS+K PP SG
Sbjct: 288  HVPISQRMKKSATSGNKSSTAKQKVTKVVSSSMKKASKKFKKVVKKSKYSKSSKVPPSSG 347

Query: 1924 EGQKWTTLVHNGVIFPPPYKPHGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKF 1745
            EG+KW TLVHNGVIFPPPYKPHGVKM+Y GKPVDLTPEQEEVATMFAVM DTDY TK KF
Sbjct: 348  EGKKWNTLVHNGVIFPPPYKPHGVKMLYKGKPVDLTPEQEEVATMFAVMLDTDYMTKSKF 407

Query: 1744 LENFMTDWRTVLGKNHIIKKFELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXX 1565
             ENFM DWR +LGKNH+I+  E CDFTPIYEWHQ EKEKKK+M+                
Sbjct: 408  KENFMDDWRKILGKNHVIQNLEDCDFTPIYEWHQREKEKKKKMTAEEKKVLKEERLKQEE 467

Query: 1564 KYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECS 1385
            KYMWAIV+GVKEKVGNFRVEPP LFRGRGEHPKMGKLKK IRP DITINIGK AP+PEC 
Sbjct: 468  KYMWAIVNGVKEKVGNFRVEPPSLFRGRGEHPKMGKLKKCIRPSDITINIGKDAPIPECP 527

Query: 1384 IPGERWKEVKHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYI 1205
            IPGE WKE++HDNTVTWLAFWNDPI P+EFKYVFLAASSSLKGQSDKEKYEKARLLK++I
Sbjct: 528  IPGESWKEIRHDNTVTWLAFWNDPIKPREFKYVFLAASSSLKGQSDKEKYEKARLLKDFI 587

Query: 1204 ENIRVNYAKDFTNKDVVKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLV 1025
            + IR  Y KDF +KD+ KRQ+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+  
Sbjct: 588  QGIRAAYTKDFASKDITKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVEPK 647

Query: 1024 PPNKLEFDFLGKDSVRYFNTVDVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKEL 845
            PP+ L+FDFLGKDS+RY N V+VE+PVYKAI QF++GK+ GDDLFDKLDTSKLNAHLK L
Sbjct: 648  PPSILKFDFLGKDSIRYQNEVEVELPVYKAIQQFRTGKRGGDDLFDKLDTSKLNAHLKGL 707

Query: 844  MPGLTAKVFRTFNASITLDDMLNKETRGGDVAQKIVVYQHANKEVAIICNHQRSVSKSHS 665
            MPGLTAKVFRT+NASITLD+ML++ET+GG+VA+KIVVYQHANKEVAIICNHQR+VSKSH 
Sbjct: 708  MPGLTAKVFRTYNASITLDEMLSRETKGGNVAEKIVVYQHANKEVAIICNHQRTVSKSHP 767

Query: 664  AQMDKLTEKMNXXXXXXXXXXXXXARARKGKPPLKDADGKTKKNLTPEALERKIAQTNTK 485
            AQM +L  K++             ARA+KGKPPL+DADGK K+NLTPEALERKI QTN K
Sbjct: 768  AQMTRLNGKIDELKGILDGLQTDLARAKKGKPPLEDADGKPKRNLTPEALERKIGQTNAK 827

Query: 484  IEKMERDRQTKEDLKTVALGTSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMD 305
            IEKMERD++TKE LKT+ALGTSKI+YLDPRI+VAWCKRHEVPIEK+FNKSLLAKFAW+MD
Sbjct: 828  IEKMERDKETKEGLKTIALGTSKISYLDPRITVAWCKRHEVPIEKVFNKSLLAKFAWSMD 887

Query: 304  VEPDFRF 284
            V+P FRF
Sbjct: 888  VDPSFRF 894


>dbj|BAG31377.1| topoisomerase I [Catharanthus roseus]
          Length = 910

 Score = 1025 bits (2650), Expect = 0.0
 Identities = 549/928 (59%), Positives = 643/928 (69%), Gaps = 38/928 (4%)
 Frame = -1

Query: 2953 MAVDACVKHKFMXXXXXXDAPVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPSRKQVSDAL 2774
            MAV+AC            D P+VFKR+ + +KQ+Q ++E  + SS K D  S +Q+ D+ 
Sbjct: 1    MAVEACPTPNLRDDMDEDDEPIVFKRNNSASKQSQTNSETRKVSSQKRDGQSVRQIYDSR 60

Query: 2773 SPNGQKSTIQQGK--------------EGSSSKATAAKTHVTSSNDSTLVADKSIHLG-- 2642
              NG+ S+ Q+G+                  SK +  K+ ++S   ST  A KS      
Sbjct: 61   PANGESSSGQKGRMVPPSKPSQVKSPLSSPPSKPSQVKSPLSSPKVSTSSAKKSPVSSLP 120

Query: 2641 -----TTLG-------NNNLEKKYLNKRHAEPT-------SDSEDSDDKMPLSFRLPAGS 2519
                 +T G       N +     + +  + P        +DSEDSDD  PLS R  A S
Sbjct: 121  ANSKPSTSGSFKVKPFNQHKSATVIKEEKSSPLRLASAIINDSEDSDDGKPLSLRHSASS 180

Query: 2518 AAPPKSNSPSVLKGLTXXXXXXXXSEKPKVSVKSEDSEDDMPLSSKFHLKSGAGASVKKP 2339
            +   K N+  V K             KP+    +EDS+D+ PLSS+F LKSG G S  K 
Sbjct: 181  S---KGNTNQVSK----------EGSKPE----NEDSDDEKPLSSRFPLKSGVGESTSKA 223

Query: 2338 YDSDEDKPLSSKIQQNGNVKRDNESSQTPKNSNKRPLGDANTSAQXXXXXXXXXXXXXXX 2159
            YDSD+DKPL+ K+Q NG+  RD + ++    SNKRP GD  +S Q               
Sbjct: 224  YDSDDDKPLAKKLQHNGSAMRDGQPNKYSNMSNKRPPGDIKSSNQLSVKKPKLSSVTAPT 283

Query: 2158 XXXXXXXKAEPXXXXXXXD-VPIAQRMTKSVTSDSKSKPMKQTPXXXXXXXXXXXXXXXX 1982
                   K EP       D +PI+QR+ KS TS+SKS  +K+T                 
Sbjct: 284  NKKQASVKPEPKAEDDDDDHLPISQRIKKSPTSESKSSSIKKTTKVVSSSVKKVNKKSKK 343

Query: 1981 XXXXXXKYLKSTKTPPGSGEGQKWTTLVHNGVIFPPPYKPHGVKMIYNGKPVDLTPEQEE 1802
                   Y KS+K PP SGEG+KW  L HNGVIFPPPYKPHG+KM+Y GKPVDLTPEQEE
Sbjct: 344  VMKNSK-YSKSSKVPPSSGEGKKWNKLEHNGVIFPPPYKPHGIKMLYKGKPVDLTPEQEE 402

Query: 1801 VATMFAVMKDTDYATKPKFLENFMTDWRTVLGKNHIIKKFELCDFTPIYEWHQNEKEKKK 1622
            VATMFAVM DTDY  KP+F ENF  DW+ +LGKNH+I+  E CDF+PIYEWHQ+EKEKKK
Sbjct: 403  VATMFAVMLDTDYMNKPRFKENFFGDWKKILGKNHVIQNLENCDFSPIYEWHQSEKEKKK 462

Query: 1621 QMSTXXXXXXXXXXXXXXXKYMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI 1442
            QM+T               KYMWA VDG KEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI
Sbjct: 463  QMTTEEKKALKEEKLKQEEKYMWAWVDGKKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI 522

Query: 1441 RPRDITINIGKGAPVPECSIPGERWKEVKHDNTVTWLAFWNDPINPKEFKYVFLAASSSL 1262
            RP DITINIGK AP+PEC +PGERWKEV+HDNTVTWLAFWNDPINPKEFKYVFLAASS+L
Sbjct: 523  RPCDITINIGKDAPIPECPVPGERWKEVRHDNTVTWLAFWNDPINPKEFKYVFLAASSTL 582

Query: 1261 KGQSDKEKYEKARLLKEYIENIRVNYAKDFTNKDVVKRQVAVATYLIDKLALRAGNEKDD 1082
            KG SDKEKYEKARLLK+YI+ IR  Y KDF +KD  K+Q+AVATYLIDKLALRAGNEKDD
Sbjct: 583  KGLSDKEKYEKARLLKDYIQGIRAAYTKDFASKDPTKKQIAVATYLIDKLALRAGNEKDD 642

Query: 1081 DEADTVGCCTLKVENVKLVPPNKLEFDFLGKDSVRYFNTVDVEVPVYKAIGQFKSGKKEG 902
            DEADTVGCCTLKVENV+ VPPN L+FDFLGKDS+RY N V+VE  V+KAI QF+SGK+  
Sbjct: 643  DEADTVGCCTLKVENVEPVPPNILKFDFLGKDSIRYQNEVEVEAAVFKAIQQFRSGKEGS 702

Query: 901  DDLFDKLDTSKLNAHLKELMPGLTAKVFRTFNASITLDDMLNKETRGGDVAQKIVVYQHA 722
             DLFD LDTSKLNAHLKELMPGLTAKVFRT+NASITLDDML++ET+GGDVA+K+VVYQHA
Sbjct: 703  SDLFDLLDTSKLNAHLKELMPGLTAKVFRTYNASITLDDMLSRETKGGDVAEKVVVYQHA 762

Query: 721  NKEVAIICNHQRSVSKSHSAQMDKLTEKMNXXXXXXXXXXXXXARARKGKPPL--KDADG 548
            NKEVAIICNHQR+VSKSHSAQM +L EK+              +R +KGKPPL  K+ADG
Sbjct: 763  NKEVAIICNHQRTVSKSHSAQMLRLNEKIEELKAVVEELKSDLSRVKKGKPPLKSKNADG 822

Query: 547  KTKKNLTPEALERKIAQTNTKIEKMERDRQTKEDLKTVALGTSKINYLDPRISVAWCKRH 368
            K K+NL PE+LE+KIAQTN KIEKMERD++TKEDLKTVALGTSKINYLDPRI+VAWCKRH
Sbjct: 823  KPKRNLNPESLEKKIAQTNAKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRH 882

Query: 367  EVPIEKIFNKSLLAKFAWAMDVEPDFRF 284
            EVPIEKIFNKSLLAKF WAMDV+P FRF
Sbjct: 883  EVPIEKIFNKSLLAKFTWAMDVDPSFRF 910


>emb|CBI28783.3| unnamed protein product [Vitis vinifera]
          Length = 724

 Score = 1008 bits (2605), Expect = 0.0
 Identities = 533/778 (68%), Positives = 581/778 (74%), Gaps = 5/778 (0%)
 Frame = -1

Query: 2602 NKRHAEP---TSDSEDSDDKMPLSFRLPAGSAAPPKSNSPSVLKGLTXXXXXXXXSEKPK 2432
            NK+   P     +SEDS+D  PLS +L   SA  PK NS  VLKGL+         +K K
Sbjct: 3    NKQLINPPVANGESEDSEDDKPLSAKL---SARLPKGNS-HVLKGLSSPVLQS---QKTK 55

Query: 2431 VSVKSEDSEDDMPLSSKFHLKSGAGASVKKPYDSDE-DKPLSSKIQQNGNVKRDNESSQT 2255
            V   S+DSED++PL  K  LKS  GAS  K  DS     P SS+ Q           SQ 
Sbjct: 56   VD-SSDDSEDEIPLLLKLQLKSRKGASSSKLLDSKGLSSPASSRPQ-----------SQK 103

Query: 2254 PKNSNKRPLGDANTSAQXXXXXXXXXXXXXXXXXXXXXXKAEPXXXXXXXDVPIAQRMT- 2078
             K +NK    D+                                       +P++ +   
Sbjct: 104  AKVNNKNLSDDSEDE------------------------------------IPLSSKFRL 127

Query: 2077 KSVTSDSKSKPMKQTPXXXXXXXXXXXXXXXXXXXXXXKYLKSTKTPPGSGEGQKWTTLV 1898
            K+V+SD+K K  K                         KY KSTK PP SGEGQKWTTL 
Sbjct: 128  KTVSSDNK-KASKTKVIPSSRKKATKKAKKSKKLAKSSKYSKSTKVPPNSGEGQKWTTLE 186

Query: 1897 HNGVIFPPPYKPHGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKFLENFMTDWR 1718
            HNGVIFPPPYKPHGVKM+YNG+PVDLTPEQEEVATMFAVMKDTDYATKP+F+ENFM DWR
Sbjct: 187  HNGVIFPPPYKPHGVKMLYNGRPVDLTPEQEEVATMFAVMKDTDYATKPRFIENFMNDWR 246

Query: 1717 TVLGKNHIIKKFELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXXKYMWAIVDG 1538
              LGKNH+IKKFELCDFTPIYEWH+ EKEKKKQM+T               KYMWA+VDG
Sbjct: 247  VRLGKNHVIKKFELCDFTPIYEWHEKEKEKKKQMTTEEKKALKEEKLKQEDKYMWAVVDG 306

Query: 1537 VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECSIPGERWKEV 1358
            VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRI P DITINIGK APVPEC IPGERWKEV
Sbjct: 307  VKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRICPSDITINIGKEAPVPECPIPGERWKEV 366

Query: 1357 KHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYIENIRVNYAK 1178
            +HDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLK+YI+NIR  Y K
Sbjct: 367  RHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKDYIQNIRATYTK 426

Query: 1177 DFTNKDVVKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLVPPNKLEFDF 998
            DFTNKD +KRQ+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV LVPPNKLEFDF
Sbjct: 427  DFTNKDTMKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVALVPPNKLEFDF 486

Query: 997  LGKDSVRYFNTVDVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKELMPGLTAKVF 818
            LGKDS+RYFNTV+VE+PVYKAIGQFK+GK  GDDLFDKLDTSKLNAHLKELMPGLTAKVF
Sbjct: 487  LGKDSIRYFNTVEVELPVYKAIGQFKTGKSGGDDLFDKLDTSKLNAHLKELMPGLTAKVF 546

Query: 817  RTFNASITLDDMLNKETRGGDVAQKIVVYQHANKEVAIICNHQRSVSKSHSAQMDKLTEK 638
            RT+NASITLD+MLN+E RGGD  +KIV+YQHANKEVAIICNHQRSVSKSH  QM +L+EK
Sbjct: 547  RTYNASITLDEMLNREARGGDAMEKIVIYQHANKEVAIICNHQRSVSKSHDVQMSRLSEK 606

Query: 637  MNXXXXXXXXXXXXXARARKGKPPLKDADGKTKKNLTPEALERKIAQTNTKIEKMERDRQ 458
            M              ARA+KGKPPLKD+DGK KKN+TPE LERKIAQTNTKIEKMERD +
Sbjct: 607  MRELQGVLDELKTDLARAKKGKPPLKDSDGKPKKNMTPEVLERKIAQTNTKIEKMERDMK 666

Query: 457  TKEDLKTVALGTSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMDVEPDFRF 284
            TKEDLKTVALGTSKINYLDPRISVAWCKR EVPIEKIFNKSLLAKFAWAMDV+P FRF
Sbjct: 667  TKEDLKTVALGTSKINYLDPRISVAWCKRQEVPIEKIFNKSLLAKFAWAMDVDPSFRF 724


>ref|XP_004149585.1| PREDICTED: DNA topoisomerase 1-like [Cucumis sativus]
            gi|449503189|ref|XP_004161878.1| PREDICTED: DNA
            topoisomerase 1-like [Cucumis sativus]
          Length = 889

 Score =  993 bits (2566), Expect = 0.0
 Identities = 534/906 (58%), Positives = 631/906 (69%), Gaps = 16/906 (1%)
 Frame = -1

Query: 2953 MAVDACVKHKFMXXXXXXDAPVVFKRSKTTTKQNQLSAEVNRKSSVKSDAPSRKQVSDAL 2774
            MAV+   K          + P+VFKR+ + ++QNQL +E+    + +      +Q SD  
Sbjct: 1    MAVETFGKAPMFDDDDEDNMPIVFKRT-SASRQNQLDSEIKHSQAQRLSKLPGRQGSDLC 59

Query: 2773 SPNGQKSTIQQGKEGSSSKATAAKTHVTS---SNDSTLVADKSIHLGTTL----GNNNLE 2615
            S NGQ S  Q+ +  S  K +  ++ + S   S  S +    +  LGT+L      ++  
Sbjct: 60   STNGQSSNNQK-RMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRP 118

Query: 2614 KKYLNKRHAE------PTSDSEDSDDKMPLSFRLPAGSAAPPKSNSPSVLKGLTXXXXXX 2453
            K  +N    E      P+ ++ DS+D  PLS RL      P K    +V K         
Sbjct: 119  KSLVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKN-------- 170

Query: 2452 XXSEKPKVSVKSEDSEDDMPLSSKFHLKSGAGASVKKPYDSDEDKPLSSKIQQNGNVKRD 2273
                  KV     DS+D++PLSSKF +K  +G    K +  DE KPL  K+ QNG+  RD
Sbjct: 171  ------KVKKSLADSDDEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRD 224

Query: 2272 NESSQTPKNSNKRPLG--DANTSAQXXXXXXXXXXXXXXXXXXXXXXKAEPXXXXXXXDV 2099
             +  +    S+KRPL   D+++ +Q                      KA+        D+
Sbjct: 225  -KLQKPAVLSSKRPLPNEDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDI 283

Query: 2098 PIAQRMTKSVTSDSKSKPMKQTPXXXXXXXXXXXXXXXXXXXXXXKYLKSTKTPPGSGEG 1919
            PI+QRM K   + +KS   K T                       KY+KSTK  P +G+G
Sbjct: 284  PISQRMKKVSATVNKSSSSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDG 343

Query: 1918 QK-WTTLVHNGVIFPPPYKPHGVKMIYNGKPVDLTPEQEEVATMFAVMKDTDYATKPKFL 1742
            QK WTTLVHNGVIFPPPY+ HGVKM+Y GKPVDLTP QEEVATMFAVMKDTDY  KPKF 
Sbjct: 344  QKKWTTLVHNGVIFPPPYQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFK 403

Query: 1741 ENFMTDWRTVLGKNHIIKKFELCDFTPIYEWHQNEKEKKKQMSTXXXXXXXXXXXXXXXK 1562
            ENF  DWR +LGKNH+I+  + CDFTPIY+W+Q EKEKKKQM+T               K
Sbjct: 404  ENFWNDWRKLLGKNHVIQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEK 463

Query: 1561 YMWAIVDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKKRIRPRDITINIGKGAPVPECSI 1382
            YMWAI+DGVKEKVGNFRVEPPGLFRGRGEHPKMGKLK+RI P DITINIGKGAP+PEC I
Sbjct: 464  YMWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPI 523

Query: 1381 PGERWKEVKHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARLLKEYIE 1202
            PGERWKEV+HDNTVTWLAFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKAR+LK+YI+
Sbjct: 524  PGERWKEVRHDNTVTWLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIK 583

Query: 1201 NIRVNYAKDFTNKDVVKRQVAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKLVP 1022
            NIR  Y KDFT+KD  +RQ+AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VP
Sbjct: 584  NIRNAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVP 643

Query: 1021 PNKLEFDFLGKDSVRYFNTVDVEVPVYKAIGQFKSGKKEGDDLFDKLDTSKLNAHLKELM 842
            PN LEF+FLGKDS+RY NTV+VE+PV+KAI QF+SGK   DDLFDKLDTSKLNAHLK+LM
Sbjct: 644  PNLLEFNFLGKDSIRYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLM 703

Query: 841  PGLTAKVFRTFNASITLDDMLNKETRGGDVAQKIVVYQHANKEVAIICNHQRSVSKSHSA 662
            PGLTAKVFRT+NASITLDDMLN+ T+ G+V++KIV YQHANKEVAIICNHQR+VSKSH A
Sbjct: 704  PGLTAKVFRTYNASITLDDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGA 763

Query: 661  QMDKLTEKMNXXXXXXXXXXXXXARARKGKPPLKDADGKTKKNLTPEALERKIAQTNTKI 482
            QM +L EK++              RA+KGKPPLK +DGK K+NLTPEALE+KI+QTN KI
Sbjct: 764  QMTRLNEKISELRDILKELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKI 823

Query: 481  EKMERDRQTKEDLKTVALGTSKINYLDPRISVAWCKRHEVPIEKIFNKSLLAKFAWAMDV 302
            EK+ERD +TKEDLKTVALGTSKINYLDPRI+VAWCKRHEVPIEKIFNKSLLAKFAWAMDV
Sbjct: 824  EKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDV 883

Query: 301  EPDFRF 284
            +PDFRF
Sbjct: 884  DPDFRF 889