BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00005489
         (1559 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283889.2| PREDICTED: aspartic proteinase nepenthesin-1...   588   e-165
ref|XP_002514026.1| Aspartic proteinase nepenthesin-1 precursor,...   583   e-164
ref|XP_002302336.1| predicted protein [Populus trichocarpa] gi|2...   570   e-160
ref|XP_004148418.1| PREDICTED: aspartic proteinase nepenthesin-1...   569   e-160
gb|ABK95290.1| unknown [Populus trichocarpa]                          565   e-158

>ref|XP_002283889.2| PREDICTED: aspartic proteinase nepenthesin-1-like [Vitis vinifera]
          Length = 439

 Score =  588 bits (1516), Expect = e-165
 Identities = 285/441 (64%), Positives = 339/441 (76%), Gaps = 9/441 (2%)
 Frame = +3

Query: 114  MALAIAFLFFSILISTTTTYGLDSCTS-------SDLSIVHIYGKCSPFTSPKRESWVDT 272
            M +  AF+F ++++STT  +  D C S       SDLS++H+YG+CSPF   K  SWV+T
Sbjct: 1    MDIFTAFVFLTLVVSTTKAF--DPCASPSSESKGSDLSVIHVYGQCSPFNQHKAGSWVNT 58

Query: 273  VIDMAKKDPLRVTYLSMLIG--EKTSVPIASGQQVLNIGNYVIRAKFGTPGQLVYMVMDT 446
            VI+MA KDP RVTYLS L+   + TSVPIASGQQVLNIGNYV+R K GTPGQL++MV+DT
Sbjct: 59   VINMASKDPARVTYLSSLVASPKATSVPIASGQQVLNIGNYVVRVKLGTPGQLMFMVLDT 118

Query: 447  SNDAAWIPCSGCSGCSPTMFSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSY 626
            S DAAW+PC+ C+GCS   FSPN S+TY  + C +PQCT VRGLSCP +G + C FNQ+Y
Sbjct: 119  SRDAAWVPCADCAGCSSPTFSPNTSSTYASLQCSVPQCTQVRGLSCPTTGTAACFFNQTY 178

Query: 627  GGDSSFSATLSQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXXAL 806
            GGDSSFSA LSQDSL L +D +P Y FGC+++VSG  +PPQ                 +L
Sbjct: 179  GGDSSFSAMLSQDSLGLAVDTLPSYSFGCVNAVSGSTLPPQGLLGLGRGPMSLLSQSGSL 238

Query: 807  YSGVFSYCLPSFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVL 986
            YSGVFSYC PSFKSYYFSGSL+LGP GQPK I+TTPLLRNPHRP+LYYVNLTG+SVG+VL
Sbjct: 239  YSGVFSYCFPSFKSYYFSGSLRLGPLGQPKNIRTTPLLRNPHRPTLYYVNLTGVSVGRVL 298

Query: 987  VPVAPELLAFDPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFT 1166
            VPVAPELLAFDP+TGAGTIIDSGTVITRFV PVY A+RDEFRK + G  +++GAFDTCF 
Sbjct: 299  VPVAPELLAFDPNTGAGTIIDSGTVITRFVEPVYAAIRDEFRKQVKGPFATIGAFDTCFA 358

Query: 1167 SSNEALTPLITFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXXNVNSVLNVIANLQQQ 1346
            ++NE + P +TFHF GMDLKLP+ENTLIH                NVNSVLNVIANLQQQ
Sbjct: 359  ATNEDIAPPVTFHFTGMDLKLPLENTLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQ 418

Query: 1347 NLRILFDLTNSKLGIGRELCN 1409
            NLRI+FD+TNS+LGI RELCN
Sbjct: 419  NLRIMFDVTNSRLGIARELCN 439


>ref|XP_002514026.1| Aspartic proteinase nepenthesin-1 precursor, putative [Ricinus
            communis] gi|223547112|gb|EEF48609.1| Aspartic proteinase
            nepenthesin-1 precursor, putative [Ricinus communis]
          Length = 437

 Score =  583 bits (1502), Expect = e-164
 Identities = 284/431 (65%), Positives = 337/431 (78%), Gaps = 5/431 (1%)
 Frame = +3

Query: 132  FLFFSILISTTTTYGLDSCTS----SDLSIVHIYGKCSPFTSPKRESWVDTVIDMAKKDP 299
            FL F++L+S+T    LD C S    SDLSI+ IY KCSPF  PK+E  V+TVIDMA KDP
Sbjct: 9    FLLFALLVSSTIA--LDPCASQADDSDLSIIPIYSKCSPFIPPKQEPLVNTVIDMASKDP 66

Query: 300  LRVTYLSMLIGEKTS-VPIASGQQVLNIGNYVIRAKFGTPGQLVYMVMDTSNDAAWIPCS 476
             R+ YLS L  + T+ VPIA GQQVLNIGNYV+R K GTPGQ ++MV+DTSNDAAW+PCS
Sbjct: 67   ARLKYLSSLAAQMTTAVPIAPGQQVLNIGNYVVRVKLGTPGQFMFMVLDTSNDAAWVPCS 126

Query: 477  GCSGCSPTMFSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSYGGDSSFSATL 656
            GC+GCS T FS N S+TYG +DC M QCT VRG SCP +G S C+FNQSYGGDSSFSATL
Sbjct: 127  GCTGCSSTTFSTNTSSTYGSLDCSMAQCTQVRGFSCPATGSSSCVFNQSYGGDSSFSATL 186

Query: 657  SQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXXALYSGVFSYCLP 836
             +DSLRL  D++P++ FGC++S+SGG++PPQ                 +LYSG+FSYCLP
Sbjct: 187  VEDSLRLVNDVIPNFAFGCINSISGGSVPPQGLLGLGRGPLSLIAQSGSLYSGLFSYCLP 246

Query: 837  SFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVLVPVAPELLAF 1016
            SFKSYYFSGSLKLGP GQPK+I+ TPLLRNPHRPSLYYVNLTG+SVG+ LVP+APELLAF
Sbjct: 247  SFKSYYFSGSLKLGPAGQPKSIRYTPLLRNPHRPSLYYVNLTGVSVGRTLVPIAPELLAF 306

Query: 1017 DPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFTSSNEALTPLI 1196
            +P+TGAGTIIDSGTVITRFV P+Y A+RDEFRK ++G  SSLGAFDTCF ++NEA+ P +
Sbjct: 307  NPNTGAGTIIDSGTVITRFVQPIYTAIRDEFRKQVAGPFSSLGAFDTCFAATNEAVAPAV 366

Query: 1197 TFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXXNVNSVLNVIANLQQQNLRILFDLTN 1376
            T HF G++L LPMEN+LIH                NVNSVLNVIANLQQQNLR+LFD+ N
Sbjct: 367  TLHFTGLNLVLPMENSLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNLRLLFDVPN 426

Query: 1377 SKLGIGRELCN 1409
            S+LGI RELCN
Sbjct: 427  SRLGIARELCN 437


>ref|XP_002302336.1| predicted protein [Populus trichocarpa] gi|222844062|gb|EEE81609.1|
            predicted protein [Populus trichocarpa]
          Length = 438

 Score =  570 bits (1468), Expect = e-160
 Identities = 280/434 (64%), Positives = 329/434 (75%), Gaps = 5/434 (1%)
 Frame = +3

Query: 123  AIAFLFFSILISTTTTYGLDSCTS----SDLSIVHIYGKCSPFTSPKRESWVDTVIDMAK 290
            A  F  F++L STT    +D C +    SDLS++ IY KCSPF  PK+ESWV+TVI MA 
Sbjct: 7    AATFFLFALLFSTTKA--VDPCATQSDTSDLSVIPIYSKCSPFVPPKQESWVNTVITMAS 64

Query: 291  KDPLRVTYLSMLIGEKTS-VPIASGQQVLNIGNYVIRAKFGTPGQLVYMVMDTSNDAAWI 467
            KDP R+ YLS L  +KT+ VPIA GQQVL I NYV+R K GTPGQ ++MV+DTSNDAAW+
Sbjct: 65   KDPERLKYLSTLADQKTTAVPIAPGQQVLKIANYVVRVKLGTPGQQMFMVLDTSNDAAWV 124

Query: 468  PCSGCSGCSPTMFSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSYGGDSSFS 647
            PCSGC+GCS T F PN STT G +DC   QC+ VRG SCP +G S CLFNQSYGGDSS +
Sbjct: 125  PCSGCTGCSSTTFLPNASTTLGSLDCSGAQCSQVRGFSCPATGSSACLFNQSYGGDSSLT 184

Query: 648  ATLSQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXXALYSGVFSY 827
            ATL QD++ L  D++P + FGC+++VSGG+IPPQ                 A+YSGVFSY
Sbjct: 185  ATLVQDAITLANDVIPGFTFGCINAVSGGSIPPQGLLGLGRGPISLISQAGAMYSGVFSY 244

Query: 828  CLPSFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVLVPVAPEL 1007
            CLPSFKSYYFSGSLKLGP GQPK+I+TTPLLRNPHRPSLYYVNLTG+SVG++ VP+  E 
Sbjct: 245  CLPSFKSYYFSGSLKLGPVGQPKSIRTTPLLRNPHRPSLYYVNLTGVSVGRIKVPIPSEQ 304

Query: 1008 LAFDPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFTSSNEALT 1187
            L FDP+TGAGTIIDSGTVITRFV PVY A+RDEFRK ++G ISSLGAFDTCF ++NEA  
Sbjct: 305  LVFDPNTGAGTIIDSGTVITRFVQPVYFAIRDEFRKQVNGPISSLGAFDTCFAATNEAEA 364

Query: 1188 PLITFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXXNVNSVLNVIANLQQQNLRILFD 1367
            P IT HF+G++L LPMEN+LIH                NVNSVLNVIANLQQQNLRI+FD
Sbjct: 365  PAITLHFEGLNLVLPMENSLIHSSSGSLACLSMAAAPNNVNSVLNVIANLQQQNLRIMFD 424

Query: 1368 LTNSKLGIGRELCN 1409
             TNS+LGI RELCN
Sbjct: 425  TTNSRLGIARELCN 438


>ref|XP_004148418.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Cucumis sativus]
            gi|449518059|ref|XP_004166061.1| PREDICTED: aspartic
            proteinase nepenthesin-1-like [Cucumis sativus]
          Length = 436

 Score =  569 bits (1467), Expect = e-160
 Identities = 281/437 (64%), Positives = 329/437 (75%), Gaps = 5/437 (1%)
 Frame = +3

Query: 114  MALAIAFLFFSILISTTTTYGLDSCTSS---DLSIVHIYGKCSPFTSPKRESWVDTVIDM 284
            MAL + F F S+ +   T+   D C S    DLS++ IYGKCSPFT+PK ESW++TVIDM
Sbjct: 1    MALKL-FFFLSLALLLLTSNAFDLCASGSDGDLSVIPIYGKCSPFTAPKSESWMNTVIDM 59

Query: 285  AKKDPLRVTYLSMLIGEKT-SVPIASGQQVLNIGNYVIRAKFGTPGQLVYMVMDTSNDAA 461
            A KDP R+ YLS L  +KT + PIASGQQVLN+GNYV+R + GTPGQ +YMV+DTSNDAA
Sbjct: 60   ASKDPARIRYLSSLTAQKTVAAPIASGQQVLNVGNYVVRVQLGTPGQTMYMVLDTSNDAA 119

Query: 462  WIPCSGCSGCSPTM-FSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSYGGDS 638
            W PCSGC GCS T  FS   S+T+  +DC  P+CT  RGLSCP +G   CLFNQ+YGGDS
Sbjct: 120  WAPCSGCIGCSSTTTFSAQNSSTFATLDCSKPECTQARGLSCPTTGNVDCLFNQTYGGDS 179

Query: 639  SFSATLSQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXXALYSGV 818
            +FSATL QDSL LG +++P++ FGC+SS SG +IPPQ                 +LYSG+
Sbjct: 180  TFSATLVQDSLHLGPNVIPNFSFGCISSASGSSIPPQGLMGLGRGPLSLISQSGSLYSGL 239

Query: 819  FSYCLPSFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVLVPVA 998
            FSYCLPSFKSYYFSGSLKLGP GQPK I+TTPLL NPHRPSLYYVNLTGISVG+VLVP++
Sbjct: 240  FSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLHNPHRPSLYYVNLTGISVGRVLVPIS 299

Query: 999  PELLAFDPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFTSSNE 1178
            PELLAFDP+TGAGTIIDSGTVITRFV  +Y A+RDEFRK + G+ S LGAFDTCF ++NE
Sbjct: 300  PELLAFDPNTGAGTIIDSGTVITRFVPAIYTAVRDEFRKQVGGSFSPLGAFDTCFATNNE 359

Query: 1179 ALTPLITFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXXNVNSVLNVIANLQQQNLRI 1358
               P IT H  G+DLKLPMEN+LIH                NVNSV+NVIANLQQQN RI
Sbjct: 360  VSAPAITLHLSGLDLKLPMENSLIHSSAGSLACLAMAAAPNNVNSVVNVIANLQQQNHRI 419

Query: 1359 LFDLTNSKLGIGRELCN 1409
            LFD+ NSKLGI RELCN
Sbjct: 420  LFDINNSKLGIARELCN 436


>gb|ABK95290.1| unknown [Populus trichocarpa]
          Length = 438

 Score =  565 bits (1455), Expect = e-158
 Identities = 277/432 (64%), Positives = 327/432 (75%), Gaps = 5/432 (1%)
 Frame = +3

Query: 129  AFLFFSILISTTTTYGLDSCTS----SDLSIVHIYGKCSPFTSPKRESWVDTVIDMAKKD 296
            A  FF + +  +TT  +D C +    SDLS++ IY KCSPF  PK+ESWV+TVI MA KD
Sbjct: 7    AATFFLVALLFSTTKAVDPCATQSDTSDLSVIPIYSKCSPFVPPKQESWVNTVITMASKD 66

Query: 297  PLRVTYLSMLIGEKTS-VPIASGQQVLNIGNYVIRAKFGTPGQLVYMVMDTSNDAAWIPC 473
            P R+ YLS L  +KT+ VPIA GQQVL I NYV+R K GTPGQ ++MV+DTSNDAAW+PC
Sbjct: 67   PERLKYLSTLADQKTTAVPIAPGQQVLKIANYVVRVKLGTPGQQMFMVLDTSNDAAWVPC 126

Query: 474  SGCSGCSPTMFSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSYGGDSSFSAT 653
            SGC+G S T F PN STT G +DC   QC+ VRG SCP +G S CLFNQSYGGDSS +AT
Sbjct: 127  SGCTGFSSTTFLPNASTTLGSLDCSGAQCSQVRGFSCPATGSSACLFNQSYGGDSSLTAT 186

Query: 654  LSQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXXALYSGVFSYCL 833
            L QD++ L  D++P + FGC+++VSGG+IPPQ                 A+YSGVFSYCL
Sbjct: 187  LVQDAITLANDVIPGFTFGCINAVSGGSIPPQGLLGLGRGPISLISQAGAMYSGVFSYCL 246

Query: 834  PSFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVLVPVAPELLA 1013
            PSFKSYYFSGSLKLGP GQPK+I+TTPLLRNPHRPSLYYVNLTG+SVG++ VP+  E L 
Sbjct: 247  PSFKSYYFSGSLKLGPVGQPKSIRTTPLLRNPHRPSLYYVNLTGVSVGRIKVPIPSEQLV 306

Query: 1014 FDPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFTSSNEALTPL 1193
            FDP+TGAGTIIDSGTVITRFV PVY A+RDEFRK ++G ISSLGAFDTCF ++NEA  P 
Sbjct: 307  FDPNTGAGTIIDSGTVITRFVQPVYFAIRDEFRKQVNGPISSLGAFDTCFAATNEAEAPA 366

Query: 1194 ITFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXXNVNSVLNVIANLQQQNLRILFDLT 1373
            IT HF+G++L LPMEN+LIH                NVNSVLNVIANLQQQNLRI+FD T
Sbjct: 367  ITLHFEGLNLVLPMENSLIHSSSGSLACLSMAAAPNNVNSVLNVIANLQQQNLRIMFDTT 426

Query: 1374 NSKLGIGRELCN 1409
            NS+LGI RELCN
Sbjct: 427  NSRLGIARELCN 438