BLASTX nr result
ID: Coptis25_contig00005215
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00005215
(1710 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI37313.3| unnamed protein product [Vitis vinifera] 263 1e-67
ref|XP_002266086.1| PREDICTED: E3 ubiquitin-protein ligase PRT1-... 263 1e-67
gb|ABK94910.1| unknown [Populus trichocarpa] 241 6e-61
ref|XP_002301781.1| predicted protein [Populus trichocarpa] gi|2... 240 7e-61
ref|XP_002303829.1| predicted protein [Populus trichocarpa] gi|2... 235 3e-59
>emb|CBI37313.3| unnamed protein product [Vitis vinifera]
Length = 451
Score = 263 bits (671), Expect = 1e-67
Identities = 134/259 (51%), Positives = 173/259 (66%), Gaps = 7/259 (2%)
Frame = -2
Query: 1067 GHISCFWCVHWAMSGMGESHCPICRCPYSHFPSICQLLHFLLLKMYPVAYKRREKQMLEE 888
GHISCFWCVH++M G ESHCPICR PYSHFP+ICQ+LHF+LLKMYPVAYKRREKQ LE
Sbjct: 21 GHISCFWCVHYSMDGAHESHCPICRNPYSHFPTICQMLHFMLLKMYPVAYKRREKQTLER 80
Query: 887 EKRRGFFSPQLGDNVLRAHSCEESNCLDSPETFSGMCFQTAPYGSTCSNGKINTSSTVEK 708
E+ FSP+ +H+ ++ P S F+ C + ++S+ V+K
Sbjct: 81 EEETRCFSPRFDAYGGGSHTYKKLKIEADPADPSITVFEF----KLCPD---SSSNRVKK 133
Query: 707 QTPDILAHANASL-------LSAEAHSGSEVTDTLAVEESSILENDLKDGICKLFSSSDV 549
D++ SL +S + G+ TLA EE+ +L+N+L D C+ S +DV
Sbjct: 134 HFADLMQRQPVSLPQDFVIPISKQTSGGNPQGATLADEENVVLKNNL-DQTCEQLSIADV 192
Query: 548 LCAACKQLLFRPAVLNCGHVYCQSCIVIPVNEALRCQLCQSSHPKGIPKVCLELDHLLEK 369
C ACKQLLFRP VLNCGHVYC++C IPV+E RC++CQ HP G PKVCLELD+ LE+
Sbjct: 193 QCTACKQLLFRPVVLNCGHVYCETCFNIPVDERFRCKVCQVLHPSGFPKVCLELDNFLEE 252
Query: 368 RFPKEYALRREAVQLQPIH 312
+FP+EYALRREAVQL+ +H
Sbjct: 253 QFPEEYALRREAVQLKAVH 271
Score = 64.3 bits (155), Expect = 9e-08
Identities = 25/39 (64%), Positives = 30/39 (76%)
Frame = -3
Query: 118 SWLGDRGLNVHLGVGCDYCGMYPIVGERYKCKDCEEAIG 2
+WL D G +H GCD CGMYPIVG+RY+CKDC+E IG
Sbjct: 300 TWLRDNGPKIHPCAGCDSCGMYPIVGDRYRCKDCKEKIG 338
>ref|XP_002266086.1| PREDICTED: E3 ubiquitin-protein ligase PRT1-like [Vitis vinifera]
Length = 470
Score = 263 bits (671), Expect = 1e-67
Identities = 134/259 (51%), Positives = 173/259 (66%), Gaps = 7/259 (2%)
Frame = -2
Query: 1067 GHISCFWCVHWAMSGMGESHCPICRCPYSHFPSICQLLHFLLLKMYPVAYKRREKQMLEE 888
GHISCFWCVH++M G ESHCPICR PYSHFP+ICQ+LHF+LLKMYPVAYKRREKQ LE
Sbjct: 40 GHISCFWCVHYSMDGAHESHCPICRNPYSHFPTICQMLHFMLLKMYPVAYKRREKQTLER 99
Query: 887 EKRRGFFSPQLGDNVLRAHSCEESNCLDSPETFSGMCFQTAPYGSTCSNGKINTSSTVEK 708
E+ FSP+ +H+ ++ P S F+ C + ++S+ V+K
Sbjct: 100 EEETRCFSPRFDAYGGGSHTYKKLKIEADPADPSITVFEF----KLCPD---SSSNRVKK 152
Query: 707 QTPDILAHANASL-------LSAEAHSGSEVTDTLAVEESSILENDLKDGICKLFSSSDV 549
D++ SL +S + G+ TLA EE+ +L+N+L D C+ S +DV
Sbjct: 153 HFADLMQRQPVSLPQDFVIPISKQTSGGNPQGATLADEENVVLKNNL-DQTCEQLSIADV 211
Query: 548 LCAACKQLLFRPAVLNCGHVYCQSCIVIPVNEALRCQLCQSSHPKGIPKVCLELDHLLEK 369
C ACKQLLFRP VLNCGHVYC++C IPV+E RC++CQ HP G PKVCLELD+ LE+
Sbjct: 212 QCTACKQLLFRPVVLNCGHVYCETCFNIPVDERFRCKVCQVLHPSGFPKVCLELDNFLEE 271
Query: 368 RFPKEYALRREAVQLQPIH 312
+FP+EYALRREAVQL+ +H
Sbjct: 272 QFPEEYALRREAVQLKAVH 290
Score = 64.3 bits (155), Expect = 9e-08
Identities = 25/39 (64%), Positives = 30/39 (76%)
Frame = -3
Query: 118 SWLGDRGLNVHLGVGCDYCGMYPIVGERYKCKDCEEAIG 2
+WL D G +H GCD CGMYPIVG+RY+CKDC+E IG
Sbjct: 319 TWLRDNGPKIHPCAGCDSCGMYPIVGDRYRCKDCKEKIG 357
>gb|ABK94910.1| unknown [Populus trichocarpa]
Length = 469
Score = 241 bits (614), Expect = 6e-61
Identities = 126/260 (48%), Positives = 165/260 (63%), Gaps = 9/260 (3%)
Frame = -2
Query: 1067 GHISCFWCVHWAMSGMGESHCPICRCPYSHFPSICQLLHFLLLKMYPVAYKRREKQMLEE 888
GHISCFWCVH +MSG+ ES+CPICR PY+HFP+ICQ+LHFLL K+YP+ Y RRE+Q LEE
Sbjct: 55 GHISCFWCVHKSMSGLRESNCPICRHPYNHFPTICQMLHFLLFKLYPMVYTRREEQTLEE 114
Query: 887 EKRRGFFSPQLG-----DNVLRAHSCEESNCLDSPETFSGMCFQTAPY-GSTCSNGKINT 726
E GFFSPQ G ++ H + + LDS F+ + GST + +
Sbjct: 115 EMEMGFFSPQFGYKECNSDLKHHHPRDRKHALDS------CFFRNGEFCGSTQQIESVKS 168
Query: 725 SSTVEKQT---PDILAHANASLLSAEAHSGSEVTDTLAVEESSILENDLKDGICKLFSSS 555
S ++ T P+ + N ++ + +VEE+++ E D + CK S +
Sbjct: 169 VSMIQAPTMSIPNKVCDENCCMIKPD-----------SVEENNLPE-DKSNRNCKQVSIA 216
Query: 554 DVLCAACKQLLFRPAVLNCGHVYCQSCIVIPVNEALRCQLCQSSHPKGIPKVCLELDHLL 375
DV C+ CKQLLF P VLNCGHVYC++CI PVNE L CQ+CQS HP+G PKVCLE DH L
Sbjct: 217 DVQCSTCKQLLFHPVVLNCGHVYCETCIG-PVNEMLTCQVCQSLHPRGFPKVCLEFDHFL 275
Query: 374 EKRFPKEYALRREAVQLQPI 315
E+ FP EYA+R EAVQ + +
Sbjct: 276 EEYFPTEYAMRIEAVQAKQV 295
Score = 69.7 bits (169), Expect = 2e-09
Identities = 32/54 (59%), Positives = 36/54 (66%)
Frame = -3
Query: 163 QKCPHSSPPLTWSCLSWLGDRGLNVHLGVGCDYCGMYPIVGERYKCKDCEEAIG 2
+K SS T LSW D VH+GVGCD CG+YPIVG+RYKCKDC E IG
Sbjct: 311 EKSFQSSSATTRENLSWRADPHSKVHVGVGCDSCGVYPIVGDRYKCKDCVEEIG 364
>ref|XP_002301781.1| predicted protein [Populus trichocarpa] gi|222843507|gb|EEE81054.1|
predicted protein [Populus trichocarpa]
Length = 382
Score = 240 bits (613), Expect = 7e-61
Identities = 126/260 (48%), Positives = 165/260 (63%), Gaps = 9/260 (3%)
Frame = -2
Query: 1067 GHISCFWCVHWAMSGMGESHCPICRCPYSHFPSICQLLHFLLLKMYPVAYKRREKQMLEE 888
GHISCFWCVH +MSG+ ES+CPICR PY+HFP+ICQ+LHFLL K+YP+ Y RRE+Q LEE
Sbjct: 42 GHISCFWCVHKSMSGLRESNCPICRHPYNHFPTICQMLHFLLFKLYPMVYTRREEQTLEE 101
Query: 887 EKRRGFFSPQLG-----DNVLRAHSCEESNCLDSPETFSGMCFQTAPY-GSTCSNGKINT 726
E GFFSPQ G ++ H + + LDS F+ + GST + +
Sbjct: 102 EMEMGFFSPQFGYKECNSDLKHHHPRDRKHALDS------CFFRNGEFCGSTQQIESVKS 155
Query: 725 SSTVEKQT---PDILAHANASLLSAEAHSGSEVTDTLAVEESSILENDLKDGICKLFSSS 555
S ++ T P+ + N ++ + +VEE+++ E D + CK S +
Sbjct: 156 VSMIQAPTMSIPNKVCDENCCMIKPD-----------SVEENNLPE-DKSNRNCKQVSIA 203
Query: 554 DVLCAACKQLLFRPAVLNCGHVYCQSCIVIPVNEALRCQLCQSSHPKGIPKVCLELDHLL 375
DV C+ CKQLLF P VLNCGHVYC++CI PVNE L CQ+CQS HP+G PKVCLE DH L
Sbjct: 204 DVQCSTCKQLLFHPVVLNCGHVYCETCIG-PVNEMLTCQVCQSLHPRGFPKVCLEFDHFL 262
Query: 374 EKRFPKEYALRREAVQLQPI 315
E+ FP EYA+R EAVQ + +
Sbjct: 263 EEYFPTEYAMRIEAVQAKQL 282
Score = 69.7 bits (169), Expect = 2e-09
Identities = 32/54 (59%), Positives = 36/54 (66%)
Frame = -3
Query: 163 QKCPHSSPPLTWSCLSWLGDRGLNVHLGVGCDYCGMYPIVGERYKCKDCEEAIG 2
+K SS T LSW D VH+GVGCD CG+YPIVG+RYKCKDC E IG
Sbjct: 296 EKSFQSSSATTRENLSWRADPHSKVHVGVGCDSCGVYPIVGDRYKCKDCVEEIG 349
>ref|XP_002303829.1| predicted protein [Populus trichocarpa] gi|222841261|gb|EEE78808.1|
predicted protein [Populus trichocarpa]
Length = 469
Score = 235 bits (599), Expect = 3e-59
Identities = 120/251 (47%), Positives = 157/251 (62%), Gaps = 2/251 (0%)
Frame = -2
Query: 1067 GHISCFWCVHWAMSGMGESHCPICRCPYSHFPSICQLLHFLLLKMYPVAYKRREKQMLEE 888
GH+SCFWCV ++M+G+ ESHCPICR ++HFPS+CQLLHFLL+KMYP+AYKRRE+++ EE
Sbjct: 69 GHLSCFWCVFYSMNGLRESHCPICRHQFNHFPSVCQLLHFLLMKMYPIAYKRREREVGEE 128
Query: 887 EKRRGFFSPQLGDNVLRAHSCEESNCLDSPETFSGMCFQTAPYGSTCSNGKINTSSTVEK 708
EK+ G FSPQ + +HS EE LD P F P C
Sbjct: 129 EKKGGRFSPQFVHHPFGSHSGEE---LDFPS--YSQHFPIHPQNKLC------------- 170
Query: 707 QTPDILAH--ANASLLSAEAHSGSEVTDTLAVEESSILENDLKDGICKLFSSSDVLCAAC 534
P +AH N ++ + S S+ T A+E +++ + GI S +D+LCA C
Sbjct: 171 YFPKAIAHREENMKIVPSTLSSRSDGTTNAAIENCNLIGTEFGHGIKTQASVADLLCAEC 230
Query: 533 KQLLFRPAVLNCGHVYCQSCIVIPVNEALRCQLCQSSHPKGIPKVCLELDHLLEKRFPKE 354
K+LLF+P VLNCGHVYC+SCI P+ RCQ+CQS HP GIP VCL L+H LE++F +
Sbjct: 231 KKLLFQPVVLNCGHVYCESCIASPMQGIPRCQICQSLHPNGIPSVCLVLEHFLEEQFSEI 290
Query: 353 YALRREAVQLQ 321
YA RREA Q
Sbjct: 291 YAERREAFAKQ 301
Score = 64.3 bits (155), Expect = 9e-08
Identities = 26/39 (66%), Positives = 29/39 (74%)
Frame = -3
Query: 118 SWLGDRGLNVHLGVGCDYCGMYPIVGERYKCKDCEEAIG 2
SW+ G VH+ VGCD CGM PI+GERYKCKDC E IG
Sbjct: 327 SWIFGNGPKVHIRVGCDSCGMIPIIGERYKCKDCSEEIG 365