BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00005072
         (1516 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN65806.1| hypothetical protein VITISV_015630 [Vitis vinifera]   486   e-142
ref|XP_002265771.2| PREDICTED: aspartic proteinase nepenthesin-2...   483   e-141
emb|CBI24128.3| unnamed protein product [Vitis vinifera]              481   e-133
ref|XP_002518395.1| Aspartic proteinase Asp1 precursor, putative...   395   e-112
ref|XP_003566992.1| PREDICTED: aspartic proteinase nepenthesin-2...   386   e-105

>emb|CAN65806.1| hypothetical protein VITISV_015630 [Vitis vinifera]
          Length = 449

 Score =  486 bits (1250), Expect(2) = e-142
 Identities = 243/386 (62%), Positives = 291/386 (75%), Gaps = 9/386 (2%)
 Frame = -3

Query: 1184 SDSTGSISLLSGAYSGTGQYFVRFRVGTPSQKFLLVADTGSDLTWINCQHHCQT--C--- 1020
            SD    + +   A  G GQYFV F+VGTPSQKF+LVADTGSDLTW++C++HC++  C   
Sbjct: 64   SDDAIEVPMHPAADYGIGQYFVAFKVGTPSQKFMLVADTGSDLTWMSCKYHCRSRNCSNR 123

Query: 1019 -ARRTHGRRVFHAHHSSSFKTISCSSDMCKMTLP--FSLAQCPTPETPCQYDYRYADGSR 849
             ARR   +RVFHA+ SSSFKTI C +DMCK+ L   FSL  CPTP TPC YDYRY+DGS 
Sbjct: 124  KARRIRHKRVFHANLSSSFKTIPCLTDMCKIELMDLFSLTNCPTPLTPCGYDYRYSDGST 183

Query: 848  SRGIFANETVTVGLSSGRKTRLHNVVIGCSSTIEGSSFAASDGVLGLGYSNSSFVINARD 669
            + G FANETVTV L  GRK +LHNV+IGCS + +G SF A+DGV+GLGYS  SF I A +
Sbjct: 184  ALGFFANETVTVELKEGRKMKLHNVLIGCSESFQGQSFQAADGVMGLGYSKYSFAIKAAE 243

Query: 668  KFGGKFSYCLVDHLSPKNVTSYLTFGHSRIKDNAGLSNVQYTQLILNVSNGFYPVNVAGI 489
            KFGGKFSYCLVDHLS KNV++YLTFG SR K+ A L+N+ YT+L+L + N FY VN+ GI
Sbjct: 244  KFGGKFSYCLVDHLSHKNVSNYLTFGSSRSKE-ALLNNMTYTELVLGMVNSFYAVNMMGI 302

Query: 488  SIGGVMLNIPMNVWDVGGEGGMIIDSGTSLTILAEPAYGIVMNALKLPLIGFVK-EVEMK 312
            SIGG ML IP  VWDV G GG I+DSG+SLT L EPAY  VM AL++ L+ F K E+++ 
Sbjct: 303  SIGGAMLKIPSEVWDVKGAGGTILDSGSSLTFLTEPAYQPVMAALRVSLLKFRKVEMDIG 362

Query: 311  PFEFCFNSVGFNASLVPRLAIHFADSARLEPPVKSYVIDVSNGVKCLGFMSTDWPSVSII 132
            P E+CFNS GF  SLVPRL  HFAD A  EPPVKSYVI  ++GV+CLGF+S  WP  S++
Sbjct: 363  PLEYCFNSTGFEESLVPRLVFHFADGAEFEPPVKSYVISAADGVRCLGFVSVAWPGTSVV 422

Query: 131  GNIMQQNFLWEFDTVNKRLGFAPSSC 54
            GNIMQQN LWEFD   K+LGFAPSSC
Sbjct: 423  GNIMQQNHLWEFDLGLKKLGFAPSSC 448



 Score = 48.1 bits (113), Expect(2) = e-142
 Identities = 23/42 (54%), Positives = 30/42 (71%)
 Frame = -1

Query: 1342 MQFKLIHRHSAKVFSAPKTQLERIQELVHMDYHRIQMISRRL 1217
            M+ +LIHRHS +V   PKTQL+R++ELVH D  R  MI  +L
Sbjct: 1    MRLELIHRHSPQVMGRPKTQLQRLKELVHSDSVRQLMILHKL 42


>ref|XP_002265771.2| PREDICTED: aspartic proteinase nepenthesin-2-like [Vitis vinifera]
          Length = 449

 Score =  483 bits (1242), Expect(2) = e-141
 Identities = 242/386 (62%), Positives = 290/386 (75%), Gaps = 9/386 (2%)
 Frame = -3

Query: 1184 SDSTGSISLLSGAYSGTGQYFVRFRVGTPSQKFLLVADTGSDLTWINCQHHCQT--C--- 1020
            SD    + +   A  G GQY V F+VGTPSQKF+LVADTGSDLTW++C++HC++  C   
Sbjct: 64   SDDAIEVPMHPAADYGIGQYSVAFKVGTPSQKFMLVADTGSDLTWMSCKYHCRSRNCSNR 123

Query: 1019 -ARRTHGRRVFHAHHSSSFKTISCSSDMCKMTLP--FSLAQCPTPETPCQYDYRYADGSR 849
             ARR   +RVFHA+ SSSFKTI C +DMCK+ L   FSL  CPTP TPC YDYRY+DGS 
Sbjct: 124  KARRIRHKRVFHANLSSSFKTIPCLTDMCKIELMDLFSLTNCPTPLTPCGYDYRYSDGST 183

Query: 848  SRGIFANETVTVGLSSGRKTRLHNVVIGCSSTIEGSSFAASDGVLGLGYSNSSFVINARD 669
            + G FANETVTV L  GRK +LHNV+IGCS + +G SF A+DGV+GLGYS  SF I A +
Sbjct: 184  ALGFFANETVTVELKEGRKMKLHNVLIGCSESFQGQSFQAADGVMGLGYSKYSFAIKAAE 243

Query: 668  KFGGKFSYCLVDHLSPKNVTSYLTFGHSRIKDNAGLSNVQYTQLILNVSNGFYPVNVAGI 489
            KFGGKFSYCLVDHLS KNV++YLTFG SR K+ A L+N+ YT+L+L + N FY VN+ GI
Sbjct: 244  KFGGKFSYCLVDHLSHKNVSNYLTFGSSRSKE-ALLNNMTYTELVLGMVNSFYAVNMMGI 302

Query: 488  SIGGVMLNIPMNVWDVGGEGGMIIDSGTSLTILAEPAYGIVMNALKLPLIGFVK-EVEMK 312
            SIGG ML IP  VWDV G GG I+DSG+SLT L EPAY  VM AL++ L+ F K E+++ 
Sbjct: 303  SIGGAMLKIPSEVWDVKGAGGTILDSGSSLTFLTEPAYQPVMAALRVSLLKFRKVEMDIG 362

Query: 311  PFEFCFNSVGFNASLVPRLAIHFADSARLEPPVKSYVIDVSNGVKCLGFMSTDWPSVSII 132
            P E+CFNS GF  SLVPRL  HFAD A  EPPVKSYVI  ++GV+CLGF+S  WP  S++
Sbjct: 363  PLEYCFNSTGFEESLVPRLVFHFADGAEFEPPVKSYVISAADGVRCLGFVSVAWPGTSVV 422

Query: 131  GNIMQQNFLWEFDTVNKRLGFAPSSC 54
            GNIMQQN LWEFD   K+LGFAPSSC
Sbjct: 423  GNIMQQNHLWEFDLGLKKLGFAPSSC 448



 Score = 48.1 bits (113), Expect(2) = e-141
 Identities = 23/42 (54%), Positives = 30/42 (71%)
 Frame = -1

Query: 1342 MQFKLIHRHSAKVFSAPKTQLERIQELVHMDYHRIQMISRRL 1217
            M+ +LIHRHS +V   PKTQL+R++ELVH D  R  MI  +L
Sbjct: 1    MRLELIHRHSPQVMGRPKTQLQRLKELVHSDSVRQLMILHKL 42


>emb|CBI24128.3| unnamed protein product [Vitis vinifera]
          Length = 378

 Score =  481 bits (1237), Expect = e-133
 Identities = 239/371 (64%), Positives = 285/371 (76%), Gaps = 9/371 (2%)
 Frame = -3

Query: 1139 GTGQYFVRFRVGTPSQKFLLVADTGSDLTWINCQHHCQT--C----ARRTHGRRVFHAHH 978
            G GQY V F+VGTPSQKF+LVADTGSDLTW++C++HC++  C    ARR   +RVFHA+ 
Sbjct: 8    GIGQYSVAFKVGTPSQKFMLVADTGSDLTWMSCKYHCRSRNCSNRKARRIRHKRVFHANL 67

Query: 977  SSSFKTISCSSDMCKMTLP--FSLAQCPTPETPCQYDYRYADGSRSRGIFANETVTVGLS 804
            SSSFKTI C +DMCK+ L   FSL  CPTP TPC YDYRY+DGS + G FANETVTV L 
Sbjct: 68   SSSFKTIPCLTDMCKIELMDLFSLTNCPTPLTPCGYDYRYSDGSTALGFFANETVTVELK 127

Query: 803  SGRKTRLHNVVIGCSSTIEGSSFAASDGVLGLGYSNSSFVINARDKFGGKFSYCLVDHLS 624
             GRK +LHNV+IGCS + +G SF A+DGV+GLGYS  SF I A +KFGGKFSYCLVDHLS
Sbjct: 128  EGRKMKLHNVLIGCSESFQGQSFQAADGVMGLGYSKYSFAIKAAEKFGGKFSYCLVDHLS 187

Query: 623  PKNVTSYLTFGHSRIKDNAGLSNVQYTQLILNVSNGFYPVNVAGISIGGVMLNIPMNVWD 444
             KNV++YLTFG SR K+ A L+N+ YT+L+L + N FY VN+ GISIGG ML IP  VWD
Sbjct: 188  HKNVSNYLTFGSSRSKE-ALLNNMTYTELVLGMVNSFYAVNMMGISIGGAMLKIPSEVWD 246

Query: 443  VGGEGGMIIDSGTSLTILAEPAYGIVMNALKLPLIGFVK-EVEMKPFEFCFNSVGFNASL 267
            V G GG I+DSG+SLT L EPAY  VM AL++ L+ F K E+++ P E+CFNS GF  SL
Sbjct: 247  VKGAGGTILDSGSSLTFLTEPAYQPVMAALRVSLLKFRKVEMDIGPLEYCFNSTGFEESL 306

Query: 266  VPRLAIHFADSARLEPPVKSYVIDVSNGVKCLGFMSTDWPSVSIIGNIMQQNFLWEFDTV 87
            VPRL  HFAD A  EPPVKSYVI  ++GV+CLGF+S  WP  S++GNIMQQN LWEFD  
Sbjct: 307  VPRLVFHFADGAEFEPPVKSYVISAADGVRCLGFVSVAWPGTSVVGNIMQQNHLWEFDLG 366

Query: 86   NKRLGFAPSSC 54
             K+LGFAPSSC
Sbjct: 367  LKKLGFAPSSC 377


>ref|XP_002518395.1| Aspartic proteinase Asp1 precursor, putative [Ricinus communis]
            gi|223542240|gb|EEF43782.1| Aspartic proteinase Asp1
            precursor, putative [Ricinus communis]
          Length = 489

 Score =  395 bits (1016), Expect(2) = e-112
 Identities = 204/385 (52%), Positives = 265/385 (68%), Gaps = 10/385 (2%)
 Frame = -3

Query: 1175 TGSISLLSGAYSGTGQYFVRFRVGTPS-QKFLLVADTGSDLTWINCQHHCQTCAR-RTHG 1002
            T  I + SGA SG  QYFV  R+GTP  QKF+LV DTGSDLTW+NC++ C++C +   H 
Sbjct: 103  TAQIPIHSGADSGQSQYFVSIRIGTPRPQKFILVTDTGSDLTWMNCEYWCKSCPKPNPHP 162

Query: 1001 RRVFHAHHSSSFKTISCSSDMCKMTLP--FSLAQCPTPETPCQYDYRYADGSRSRGIFAN 828
             RVF A+ SSSF+TI CSSD CK+ L   FSL +CP P  PC +DYRY +G R+ G+FAN
Sbjct: 163  GRVFRANDSSSFRTIPCSSDDCKIELQDYFSLTECPNPNAPCLFDYRYLNGPRAIGVFAN 222

Query: 827  ETVTVGLSSGRKTRLHNVVIGCSSTIEGSSFAASDGVLGLGYSNSSFVINARDKFGGKFS 648
            ETVTVGL+  +K RL +V+IGC+ +   ++    DGV+GLGY   S  +   + FG KFS
Sbjct: 223  ETVTVGLNDHKKIRLFDVLIGCTESFNETN-GFPDGVMGLGYRKHSLALRLAEIFGNKFS 281

Query: 647  YCLVDHLSPKNVTSYLTFGHSRIKDNAGLSNVQYTQLILNVSNGFYPVNVAGISIGGVML 468
            YCLVDHLS  N  ++L+FG         L  +Q+T+L+L   N FYPVNV+GIS+GG ML
Sbjct: 282  YCLVDHLSSSNHKNFLSFGDI---PEMKLPKMQHTELLLGYINAFYPVNVSGISVGGSML 338

Query: 467  NIPMNVWDVGGEGGMIIDSGTSLTILAEPAYGIVMNALKLPLIGFVKEVEMKPFE----- 303
            +I  ++W+V G GGMI+DSGTSLT+LA  AY  V++ALK P+  F K  ++ P E     
Sbjct: 339  SISSDIWNVTGVGGMIVDSGTSLTMLAGEAYDKVVDALK-PI--FDKHKKVVPIELPELN 395

Query: 302  -FCFNSVGFNASLVPRLAIHFADSARLEPPVKSYVIDVSNGVKCLGFMSTDWPSVSIIGN 126
             FCF   GF+ + VPRL IHFAD A  +PPVKSY+IDV+ G+KCLG +  D+P  SI+GN
Sbjct: 396  NFCFEDKGFDRAAVPRLLIHFADGAIFKPPVKSYIIDVAEGIKCLGIIKADFPGSSILGN 455

Query: 125  IMQQNFLWEFDTVNKRLGFAPSSCV 51
            +MQQN LWE+D    +LGF PSSC+
Sbjct: 456  VMQQNHLWEYDLGRGKLGFGPSSCI 480



 Score = 37.7 bits (86), Expect(2) = e-112
 Identities = 24/69 (34%), Positives = 37/69 (53%), Gaps = 7/69 (10%)
 Frame = -1

Query: 1357 NGSKAMQFKLIHRHSAKVFSA------PKTQLERIQELVHMDYHRIQMISR-RLGKRRKD 1199
            N +  + F++ H HS K+ S       PK++L+  ++L+  D  R QMIS  R G RRK 
Sbjct: 38   NNNSGVWFEMFHMHSPKLKSQSKFLGPPKSRLDGTRQLLQSDNARRQMISSLRHGTRRKA 97

Query: 1198 IEIETQIQL 1172
             E+    Q+
Sbjct: 98   FEVSHTAQI 106


>ref|XP_003566992.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Brachypodium
            distachyon]
          Length = 479

 Score =  386 bits (992), Expect = e-105
 Identities = 203/403 (50%), Positives = 260/403 (64%), Gaps = 25/403 (6%)
 Frame = -3

Query: 1187 NSDSTGSISLLSGAYSGTGQYFVRFRVGTPSQKFLLVADTGSDLTWINCQH--------H 1032
            +S+S  ++ L S AY+G GQYFVRFRVGTP+Q FLLVADTGSDLTW+ C+         +
Sbjct: 75   SSESAFAMPLTSAAYTGIGQYFVRFRVGTPAQPFLLVADTGSDLTWVKCRPAKAAAASTN 134

Query: 1031 CQTCARRTHGRRVFHAHHSSSFKTISCSSDMCKMTLPFSLAQCPTPETPCQYDYRYADGS 852
              + A  +  RR F    S ++  I C+SD C  +LPFSL+ CPTP +PC YDYRY DGS
Sbjct: 135  SSSSASASSPRRAFRPEKSKTWAPIPCASDTCSKSLPFSLSTCPTPGSPCAYDYRYKDGS 194

Query: 851  RSRGIFANETVTVGLSSG--------RKTRLHNVVIGCSSTIEGSSFAASDGVLGLGYSN 696
             +RG    E+ T+ LSS         +K +L  +V+GC+ +  G SF ASDGVL LGYSN
Sbjct: 195  AARGTVGTESATIALSSSSSSSKNKVKKAKLQGLVLGCTGSYTGPSFEASDGVLSLGYSN 254

Query: 695  SSFVINARDKFGGKFSYCLVDHLSPKNVTSYLTFGHSRIKDN----AGLSNVQYTQLILN 528
             SF  +A  +FGG+FSYCLVDHLSP+N TSYLTFG +         A     + T L+L+
Sbjct: 255  VSFASHAASRFGGRFSYCLVDHLSPRNATSYLTFGPNSALSGPCPAAAGPGARQTPLVLD 314

Query: 527  V-SNGFYPVNVAGISIGGVMLNIPMNVWDVGGEGGMIIDSGTSLTILAEPAYGIVMNALK 351
                 FY V++  IS+ G +L IP +VW+V G GG+I+DSGTSLT+LA+PAY  V+ AL 
Sbjct: 315  SRMRPFYDVSIKAISVDGELLKIPRDVWEVDGGGGVIVDSGTSLTVLAKPAYRAVVAALG 374

Query: 350  LPLIGFVKEVEMKPFEFCFN----SVGFNASLVPRLAIHFADSARLEPPVKSYVIDVSNG 183
              L  F + V M PFE+C+N    S       +P+LA+HFA SARLEPP KSYVID + G
Sbjct: 375  KKLARFPR-VAMDPFEYCYNWTSPSRKDEGDDLPKLAVHFAGSARLEPPSKSYVIDAAPG 433

Query: 182  VKCLGFMSTDWPSVSIIGNIMQQNFLWEFDTVNKRLGFAPSSC 54
            VKC+G     WP +S+IGNI+QQ  LWEFD  N+RL F  S C
Sbjct: 434  VKCIGVQEGPWPGISVIGNILQQEHLWEFDLKNRRLRFKRSRC 476