BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00005058
         (918 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin...   292   7e-77
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi...   289   6e-76
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro...   288   9e-76
sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin...   288   1e-75
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   264   2e-68

>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=PsTNMT gi|67764091|gb|AAY79177.1|
           S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
           cis-N-methyltransferase [Papaver somniferum]
          Length = 358

 Score =  292 bits (748), Expect = 7e-77
 Identities = 136/230 (59%), Positives = 182/230 (79%)
 Frame = +2

Query: 227 EQPKKESMLKLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFINSLK 406
           ++ KKES  + L RL +G + +EEL++L+  Q +KRL+WGYK +++EQL+F+L+FI SLK
Sbjct: 5   DEVKKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLK 64

Query: 407 NMDISGGNTEMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLYCER 586
            M++SG   E +N+E YE+P  FL+  FGK +KQS CYF H+S TIDEAE A H+LYCER
Sbjct: 65  KMEMSG-EIETMNKETYELPSEFLEAVFGKTVKQSMCYFTHESATIDEAEEAAHELYCER 123

Query: 587 AQIKDGLTILDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNVKVI 766
           AQIKDG T+LDIGCGQG L+ YIAQ++KNCHVTG+TNS  Q N++ +Q   L L NV  I
Sbjct: 124 AQIKDGQTVLDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAI 183

Query: 767 LADVTKYETEALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHV 916
           LADVT+YE++  YDR+++IEA+EHMKN+QLF+ K+S WM K++LLFVDHV
Sbjct: 184 LADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTKESLLFVDHV 233


>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
           Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
           tetrahydroprotoberberine N-methyltransferase [Papaver
           bracteatum]
          Length = 358

 Score =  289 bits (740), Expect = 6e-76
 Identities = 134/230 (58%), Positives = 182/230 (79%)
 Frame = +2

Query: 227 EQPKKESMLKLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFINSLK 406
           ++ KKES  + L RL +G + +EEL++L+  Q +KRL+WGYK +++EQL+F+L+FI SLK
Sbjct: 5   DEVKKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLK 64

Query: 407 NMDISGGNTEMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLYCER 586
            M++SG   E +N+E YE+P  FL+  FGK +KQS CYFKH+S TIDEAE A H+LYCER
Sbjct: 65  KMEMSG-EIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATIDEAEEAAHELYCER 123

Query: 587 AQIKDGLTILDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNVKVI 766
           AQIKDG T+LDIGCGQG L+ YIA+++K CHVTG+TNS  Q N++ +Q   L L NV  I
Sbjct: 124 AQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAI 183

Query: 767 LADVTKYETEALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHV 916
           LADVT+YE++  YDR+++IEA+EHMKN+QLF+ K+S WM +++LLFVDHV
Sbjct: 184 LADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHV 233


>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
           N-methyltransferase 2; Short=PbTNMT2
           gi|226897730|gb|ACO90236.1| putative N-methyltransferase
           [Papaver bracteatum]
          Length = 358

 Score =  288 bits (738), Expect = 9e-76
 Identities = 134/229 (58%), Positives = 180/229 (78%)
 Frame = +2

Query: 227 EQPKKESMLKLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFINSLK 406
           E+ KKES  + L RL RG +++EEL++L+  QL+KRL+WGYK +++EQL+F+L+FINSLK
Sbjct: 5   EEVKKESAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFINSLK 64

Query: 407 NMDISGGNTEMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLYCER 586
            M +SG   E    E+YE+P    +  +GK++K S CYFKH+S TIDEAE A H+LYCER
Sbjct: 65  KMGMSG-QVEAFTNEVYELPTECFEAAYGKSMKLSGCYFKHESSTIDEAEEASHELYCER 123

Query: 587 AQIKDGLTILDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNVKVI 766
           AQIKDG T+LDIGCGQG L+ Y+AQ++KNCHVTG+TNS  Q N+I +Q   L L+NV VI
Sbjct: 124 AQIKDGQTVLDIGCGQGGLVLYVAQKYKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVI 183

Query: 767 LADVTKYETEALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDH 913
           LADVT+YE++  YDR+++I  +EHMKN+QLF+ K+S WM +D+LLFVDH
Sbjct: 184 LADVTQYESDKTYDRILVIGVVEHMKNMQLFIKKLSTWMAEDSLLFVDH 232


>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=EcTNMT gi|226897702|gb|ACO90222.1|
           tetrahydroprotoberberine N-methyltransferase
           [Eschscholzia californica]
          Length = 350

 Score =  288 bits (737), Expect = 1e-75
 Identities = 129/221 (58%), Positives = 176/221 (79%)
 Frame = +2

Query: 254 KLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFINSLKNMDISGGNT 433
           +++ RL +G + +EEL++L+  Q D+R++WGYKPT+E+QLAF+L+FI  LK M +SG   
Sbjct: 7   EIMGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMVMSG-EI 65

Query: 434 EMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLYCERAQIKDGLTI 613
           + +N+E YE+P  FL+  FGK +KQS CYFK ++ TIDEAE A H+LYCERAQIKDG T+
Sbjct: 66  DTMNKETYELPTAFLEAVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIKDGQTV 125

Query: 614 LDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNVKVILADVTKYET 793
           LDIGCGQG L+ YIA+++KNCHVTG+TNS  Q N+IE+Q   L L NV VI ADVTK++T
Sbjct: 126 LDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTKFDT 185

Query: 794 EALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHV 916
           +  YDR++++E +EHMKNIQLF+ K+S WM +D+LLFVDH+
Sbjct: 186 DKTYDRILVVETIEHMKNIQLFMKKLSTWMTEDSLLFVDHI 226


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
           gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
           [Thalictrum flavum]
          Length = 356

 Score =  264 bits (674), Expect = 2e-68
 Identities = 124/232 (53%), Positives = 175/232 (75%)
 Frame = +2

Query: 218 MDSEQPKKESMLKLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFIN 397
           M+++Q KKE++  L+ R++ G VS+EE+R ++ IQ+ KRLKWGYKPT+E+QLA  + F  
Sbjct: 1   METKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQ 60

Query: 398 SLKNMDISGGNTEMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLY 577
           SLK M+++    + ++ ELYE+P+PFL +  GK LK S  YFK +S T+DE+E  M DLY
Sbjct: 61  SLKGMEMAE-EVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLY 119

Query: 578 CERAQIKDGLTILDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNV 757
           CERAQIKDG +ILD+GCG G+L  ++AQ+++ C VTGITNS +QK FI +QC+ L+L NV
Sbjct: 120 CERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNV 179

Query: 758 KVILADVTKYETEALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDH 913
           ++IL DVTK+ETE  YDR+  +  +EHMKN +LFL K+S W+ +  LLFV+H
Sbjct: 180 EIILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEH 231