BLASTX nr result
ID: Coptis25_contig00005013
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00005013
(1792 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 545 e-152
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 545 e-152
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 545 e-152
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 543 e-152
ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222... 531 e-148
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 545 bits (1404), Expect = e-152
Identities = 267/467 (57%), Positives = 352/467 (75%), Gaps = 2/467 (0%)
Frame = +3
Query: 201 IKSPSNLPPGPFPWPIIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAA 380
+KSP LPPGP PWPI+GN+L++GN PHISLAR +++YGPLISLRLG+Q++VV S+ +AA
Sbjct: 39 LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 97
Query: 381 TEILKTHDRILSARCVPHVIPVYPDQIH-LSMAW-VDCSDQWRYLRTLCRTELFSAKAIA 554
EILKTHDR+LS R VPH +P +I+ +S+ W V+C+ W+ LRT+CR ELFS K +
Sbjct: 98 MEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVME 157
Query: 555 SQASVRDKKTNELMEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGL 734
SQA V +KK E++ F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++++DGG+
Sbjct: 158 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGM 217
Query: 735 KGLLRRFAELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFRVITKQRREDKGND 914
KGL+R+ + PNL DFY I SGLDLQG+ KK+KE+ +I ++ + ++RRE +D
Sbjct: 218 KGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 277
Query: 915 VSRQRXXXXXXXXXXXXXXQINYLIQELFLAGADTSSTTVEWAMTELIKNPKAMRKVCAE 1094
S+Q QIN L EL AGADTSS+T+EWAM ELIK+P++M+KV E
Sbjct: 278 PSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEE 336
Query: 1095 LERETSGHIVKESELPHLPYLRSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQV 1274
L RE S ++ K S+LPHLPYL++ V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+
Sbjct: 337 LAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQI 396
Query: 1275 LVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAAQQVT 1454
VNAWAIGRDP WEDPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA+ +
Sbjct: 397 WVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLP 456
Query: 1455 LTLACLINCFDWSLPHDMLLSELDMDDNFGITSQKKKPLVLIPKVRK 1595
L LA L + FDWSLP+ ELDM+D FG+T QK++PL++IPKVRK
Sbjct: 457 LILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKVRK 503
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 545 bits (1403), Expect = e-152
Identities = 268/467 (57%), Positives = 351/467 (75%), Gaps = 2/467 (0%)
Frame = +3
Query: 201 IKSPSNLPPGPFPWPIIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAA 380
+KSP LPPGP PWPI+GN+L++GN PHISLAR +++YGPLISLRLG+Q++VV S+ +AA
Sbjct: 472 LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 530
Query: 381 TEILKTHDRILSARCVPHVIPVYPDQIH-LSMAW-VDCSDQWRYLRTLCRTELFSAKAIA 554
EILKTHDR+LS R VPH +P +I+ +S+ W V+C+D W+ LRT+CR ELFS K +
Sbjct: 531 MEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVME 590
Query: 555 SQASVRDKKTNELMEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGL 734
SQA V +KK E++ F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++ +DGG+
Sbjct: 591 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGM 650
Query: 735 KGLLRRFAELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFRVITKQRREDKGND 914
KGL+R+ + PNL DFY I SGLDLQG+ KK+KE+ +I ++ + ++RRE +D
Sbjct: 651 KGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 710
Query: 915 VSRQRXXXXXXXXXXXXXXQINYLIQELFLAGADTSSTTVEWAMTELIKNPKAMRKVCAE 1094
S+Q QIN L EL AGADTSS+T+EWAM ELIK+P++M+KV E
Sbjct: 711 PSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEE 769
Query: 1095 LERETSGHIVKESELPHLPYLRSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQV 1274
L RE S ++ K S+LPHLPYL++ V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+
Sbjct: 770 LAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQI 829
Query: 1275 LVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAAQQVT 1454
VNAWAIGRDP WEDPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA+ +
Sbjct: 830 WVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLP 889
Query: 1455 LTLACLINCFDWSLPHDMLLSELDMDDNFGITSQKKKPLVLIPKVRK 1595
L LA L + FDWSLP+ ELDM+D FGI QK++PL++IPKVRK
Sbjct: 890 LILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 936
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 545 bits (1403), Expect = e-152
Identities = 268/467 (57%), Positives = 351/467 (75%), Gaps = 2/467 (0%)
Frame = +3
Query: 201 IKSPSNLPPGPFPWPIIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAA 380
+KSP LPPGP PWPI+GN+L++GN PHISLAR +++YGPLISLRLG+Q++VV S+ +AA
Sbjct: 39 LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 97
Query: 381 TEILKTHDRILSARCVPHVIPVYPDQIH-LSMAW-VDCSDQWRYLRTLCRTELFSAKAIA 554
EILKTHDR+LS R VPH +P +I+ +S+ W V+C+D W+ LRT+CR ELFS K +
Sbjct: 98 MEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVME 157
Query: 555 SQASVRDKKTNELMEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGL 734
SQA V +KK E++ F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++ +DGG+
Sbjct: 158 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGM 217
Query: 735 KGLLRRFAELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFRVITKQRREDKGND 914
KGL+R+ + PNL DFY I SGLDLQG+ KK+KE+ +I ++ + ++RRE +D
Sbjct: 218 KGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 277
Query: 915 VSRQRXXXXXXXXXXXXXXQINYLIQELFLAGADTSSTTVEWAMTELIKNPKAMRKVCAE 1094
S+Q QIN L EL AGADTSS+T+EWAM ELIK+P++M+KV E
Sbjct: 278 PSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEE 336
Query: 1095 LERETSGHIVKESELPHLPYLRSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQV 1274
L RE S ++ K S+LPHLPYL++ V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+
Sbjct: 337 LAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQI 396
Query: 1275 LVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAAQQVT 1454
VNAWAIGRDP WEDPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA+ +
Sbjct: 397 WVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLP 456
Query: 1455 LTLACLINCFDWSLPHDMLLSELDMDDNFGITSQKKKPLVLIPKVRK 1595
L LA L + FDWSLP+ ELDM+D FGI QK++PL++IPKVRK
Sbjct: 457 LILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 503
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 543 bits (1398), Expect = e-152
Identities = 266/467 (56%), Positives = 351/467 (75%), Gaps = 2/467 (0%)
Frame = +3
Query: 201 IKSPSNLPPGPFPWPIIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAA 380
+KSP LPPGP PWPI+GN+L++GN PHISLAR +++YGPLISLRLG+Q++VV S+ +AA
Sbjct: 603 LKSPP-LPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAA 661
Query: 381 TEILKTHDRILSARCVPHVIPVYPDQIH-LSMAW-VDCSDQWRYLRTLCRTELFSAKAIA 554
EILKTHDR+LS R VPH +P +I+ +S+ W V+C+ W+ LRT+CR ELFS K +
Sbjct: 662 MEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVME 721
Query: 555 SQASVRDKKTNELMEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGL 734
SQA V +KK E++ F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++++DGG+
Sbjct: 722 SQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGM 781
Query: 735 KGLLRRFAELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFRVITKQRREDKGND 914
KGL+R+ + PNL DFY I SGLDLQG+ KK+KE+ +I ++ + ++RRE +D
Sbjct: 782 KGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDD 841
Query: 915 VSRQRXXXXXXXXXXXXXXQINYLIQELFLAGADTSSTTVEWAMTELIKNPKAMRKVCAE 1094
S+Q QIN L EL AGADTSS+T+EWAM ELIK+P++M+KV E
Sbjct: 842 PSKQ-DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEE 900
Query: 1095 LERETSGHIVKESELPHLPYLRSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQV 1274
L RE S ++ K S+LPHLPYL++ V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+
Sbjct: 901 LAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQI 960
Query: 1275 LVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAAQQVT 1454
VNAWAIGRDP WEDPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA+ +
Sbjct: 961 WVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLP 1020
Query: 1455 LTLACLINCFDWSLPHDMLLSELDMDDNFGITSQKKKPLVLIPKVRK 1595
L LA L + FDWSLP+ ELDM+D FG+T QK++PL++IPK RK
Sbjct: 1021 LILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKKRK 1067
>ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222845316|gb|EEE82863.1|
cytochrome P450 [Populus trichocarpa]
Length = 507
Score = 531 bits (1367), Expect = e-148
Identities = 264/471 (56%), Positives = 344/471 (73%), Gaps = 13/471 (2%)
Frame = +3
Query: 222 PPGPFPWPIIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAATEILKTH 401
PPGP+PWPI+GNIL +G+KPHI+L AK +GP+ SLRLGTQLVVVGSSQAAA ILKTH
Sbjct: 40 PPGPYPWPILGNILQLGDKPHITLTHFAKIHGPIFSLRLGTQLVVVGSSQAAAIAILKTH 99
Query: 402 DRILSARCVPHVIPVYPDQIH-LSMAWV-DCSDQWRYLRTLCRTELFSAKAIASQASVRD 575
DRILS R VPH+ P +++ LS+ WV +C+++WRYLRT+C++ELFS KA+ SQA +R+
Sbjct: 100 DRILSGRHVPHMAPSKSSELNKLSLGWVVECNERWRYLRTICKSELFSLKALESQACIRE 159
Query: 576 KKTNELMEFLAKKEGKLVNIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGLKGLLRRF 755
+K E++ F+ K EGK+V I EV ATVFNMLSNI VS+D +SLE ESEDGG+ +L+
Sbjct: 160 RKAKEMIGFINKMEGKVVKIREVATATVFNMLSNILVSRDLVSLEHESEDGGMSSVLKDI 219
Query: 756 AELGSTPNLADFYPILSGLDLQGITKKSKEMFGQIIGVFRVITKQRRED-----KGNDVS 920
A L STPN++DFYPIL LDLQG+ KK+ E+ + + I ++RRE G D S
Sbjct: 220 ARLASTPNISDFYPILGPLDLQGLRKKTMELHRRSFNMCEAIIQERREGGEGKRDGPDAS 279
Query: 921 RQRXXXXXXXXXXXXXXQINYLIQELFLAGADTSSTTVEWAMTELIKNPKAMRKVCAEL- 1097
R+R QI+ L+ EL AG DTSS+T+EW M ELIKNP+ ++KV E+
Sbjct: 280 RRRDFLDALILNGSSDDQIDILLMELLSAGTDTSSSTIEWTMAELIKNPRCLKKVQEEIA 339
Query: 1098 -----ERETSGHIVKESELPHLPYLRSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPK 1262
R+T KES LP L YL++ V ETLRLHPP PFLLPHRA+++C+V+NYTIPK
Sbjct: 340 NVINMNRDTG---FKESHLPQLTYLQACVKETLRLHPPGPFLLPHRAIDSCQVMNYTIPK 396
Query: 1263 DSQVLVNAWAIGRDPSTWEDPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAA 1442
++QVLVN WAIGRDP +WE+P+ F PERFL S LD ++F+FIPFG+GRRICPGLPMAA
Sbjct: 397 NTQVLVNYWAIGRDPKSWEEPVVFNPERFLSSNLDFKGNDFEFIPFGSGRRICPGLPMAA 456
Query: 1443 QQVTLTLACLINCFDWSLPHDMLLSELDMDDNFGITSQKKKPLVLIPKVRK 1595
+ V L +A LI FDWSLP ++LDM +N+G+T +K++PL+L+P +K
Sbjct: 457 KHVALIIAYLILFFDWSLPCGKNPTDLDMSENYGLTLRKEQPLLLVPTSKK 507