BLASTX nr result
ID: Coptis25_contig00004142
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00004142
(1833 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002273390.1| PREDICTED: flavonoid 3'-monooxygenase-like [... 756 0.0
ref|XP_002303241.1| cytochrome P450 [Populus trichocarpa] gi|222... 744 0.0
gb|ADC34701.1| flavonoid 3' hydroxylase [Actinidia chinensis] 742 0.0
ref|XP_002521317.1| flavonoid 3-hydroxylase, putative [Ricinus c... 742 0.0
ref|XP_002331194.1| cytochrome P450 [Populus trichocarpa] gi|222... 737 0.0
>ref|XP_002273390.1| PREDICTED: flavonoid 3'-monooxygenase-like [Vitis vinifera]
Length = 511
Score = 756 bits (1951), Expect = 0.0
Identities = 362/483 (74%), Positives = 414/483 (85%), Gaps = 1/483 (0%)
Frame = +1
Query: 190 RRRCQNLPPGPKPWPIIGNFNLIGSLPHRSIHDLSQKYGPLMHIKFGSRPVVVASSVEMA 369
RRR NLPPGPKPWPIIGN NLIG+LPHRSIH+LSQKYGP+M ++FGS PVVV SSV MA
Sbjct: 28 RRRKLNLPPGPKPWPIIGNLNLIGALPHRSIHELSQKYGPIMQLRFGSFPVVVGSSVAMA 87
Query: 370 KQFLKTHDVVFAGRPQTAAGKYTTYNFSDITWSPYGPYWRQARKMCIMELFSSKXXXXXX 549
K FLKTHDV FA RP+TAAGKYTTYN+SDITWSPYGPYWRQARKMC+MELFS++
Sbjct: 88 KLFLKTHDVTFASRPKTAAGKYTTYNYSDITWSPYGPYWRQARKMCLMELFSARRLESYE 147
Query: 550 XXXXXXTRALLKGLFNSFGNPVSLKDHLSDVSLNVISRIVFGKKYLDEQ-ETSIITPNEF 726
T++LL L+ +PV LKDHLS VSLNVISR+V GKKYLDE E SI+TP EF
Sbjct: 148 YIRVEETKSLLSSLYKQSNSPVDLKDHLSTVSLNVISRMVLGKKYLDENVEGSIVTPEEF 207
Query: 727 KKMLDELFLLNGVLNIGDWIPWVGFMDLQGYVKRMKVLAKKFDRFLEHVIDEHNEKRHGV 906
KKMLDELFLL+GVLNIGD IPW+ F+DLQGYVKRMKVL+KKFDRFLEHV+DEHN +R GV
Sbjct: 208 KKMLDELFLLSGVLNIGDSIPWIDFLDLQGYVKRMKVLSKKFDRFLEHVLDEHNARRKGV 267
Query: 907 KAWVPKDMIDVLLELADDPNLDVKLTRTSVKAFSQDLIAGGTESSAVTVEWAIAELLKHP 1086
+ +V KDM+DVLL+ ADDP L+VKL R VKAF+QDLIAGGTESSAVTVEWAI+ELL+ P
Sbjct: 268 ENYVAKDMVDVLLQFADDPTLEVKLERHGVKAFTQDLIAGGTESSAVTVEWAISELLRKP 327
Query: 1087 KIFQKATEELDRVIGKERWIEEKDMPQLPYIEAIMKETMRLHPVAPMLVPRMTRENLNIA 1266
++F KATEELDRVIGKERW+EEKD+P LPY++ I+KETMR+HPVAPMLVPR +RE++ IA
Sbjct: 328 ELFDKATEELDRVIGKERWVEEKDIPNLPYLDTIVKETMRMHPVAPMLVPRFSREDIKIA 387
Query: 1267 GYDIPAGTGALVSVWTIGRDPTLWEKPEEFYPERFIGKSIDVKGNDFELLPFGAGRRMCP 1446
YDIP T LV+VWTIGRDP +W++P EF PERFIGK+IDVKG DFELLPFG GRRMCP
Sbjct: 388 DYDIPKDTRVLVNVWTIGRDPEIWDQPNEFIPERFIGKNIDVKGQDFELLPFGTGRRMCP 447
Query: 1447 GYTLGLKVIQSTLANLLHGFSWKLPENMKLEDLNMEEIFGLSTPKRFPLVVVVEPRLPAH 1626
GY+LGLKVIQS+LANLLHGF WKLP +MK DL+MEEIFGLSTPK+ PLV + EPRLPAH
Sbjct: 448 GYSLGLKVIQSSLANLLHGFKWKLPGDMKPGDLSMEEIFGLSTPKKIPLVAMAEPRLPAH 507
Query: 1627 LYD 1635
LYD
Sbjct: 508 LYD 510
>ref|XP_002303241.1| cytochrome P450 [Populus trichocarpa] gi|222840673|gb|EEE78220.1|
cytochrome P450 [Populus trichocarpa]
Length = 512
Score = 744 bits (1921), Expect = 0.0
Identities = 354/487 (72%), Positives = 409/487 (83%), Gaps = 3/487 (0%)
Frame = +1
Query: 187 LRRRCQNLPPGPKPWPIIGNFNLIGSLPHRSIHDLSQKYGPLMHIKFGSRPVVVASSVEM 366
LRRR N PPGPK WPIIGN NLIG LPHRS+H LSQKYGPLM +KFGS PVVV SSVEM
Sbjct: 26 LRRRKLNPPPGPKSWPIIGNLNLIGELPHRSLHALSQKYGPLMQVKFGSFPVVVGSSVEM 85
Query: 367 AKQFLKTHDVVFAGRPQTAAGKYTTYNFSDITWSPYGPYWRQARKMCIMELFSSKXXXXX 546
AK LKTHDV+F+GRP+TAAGKYTTYN+SDITWSPYGPYWRQARKMC+MELFS+K
Sbjct: 86 AKTILKTHDVIFSGRPKTAAGKYTTYNYSDITWSPYGPYWRQARKMCLMELFSAKRLESY 145
Query: 547 XXXXXXXTRALLKGLFNSFGNPVSLKDHLSDVSLNVISRIVFGKKYL---DEQETSIITP 717
RALLK L S G P++LKDHL+DVSLNVISR+V GKKY E E I+TP
Sbjct: 146 EYIRVEELRALLKTLNKSSGRPINLKDHLADVSLNVISRMVLGKKYTVKSSENEKEIVTP 205
Query: 718 NEFKKMLDELFLLNGVLNIGDWIPWVGFMDLQGYVKRMKVLAKKFDRFLEHVIDEHNEKR 897
EFK+MLDELFLLNGVL+IGD IPW+ F+DLQGY+KRMK L+KKFD+F+EHV+DEH +R
Sbjct: 206 EEFKEMLDELFLLNGVLDIGDSIPWIAFLDLQGYIKRMKTLSKKFDKFMEHVLDEHEARR 265
Query: 898 HGVKAWVPKDMIDVLLELADDPNLDVKLTRTSVKAFSQDLIAGGTESSAVTVEWAIAELL 1077
K W PKDM+DVLL+LA DPNL++KL R VKAFSQDLIAGGTESSAVTVEW I+E+L
Sbjct: 266 KEDKNWEPKDMVDVLLQLASDPNLEIKLERHGVKAFSQDLIAGGTESSAVTVEWGISEIL 325
Query: 1078 KHPKIFQKATEELDRVIGKERWIEEKDMPQLPYIEAIMKETMRLHPVAPMLVPRMTRENL 1257
+ P++F+KATEELDRVIG+ERW+EEKDM LPYI AI KE MRLHPVAPMLVPR RE++
Sbjct: 326 RKPEVFEKATEELDRVIGRERWVEEKDMVNLPYIYAIAKEVMRLHPVAPMLVPRAAREDI 385
Query: 1258 NIAGYDIPAGTGALVSVWTIGRDPTLWEKPEEFYPERFIGKSIDVKGNDFELLPFGAGRR 1437
NI GYDI G+ LV+VWTIGRDP +W+KP+EF+PERFIG SIDV+G+D+ELLPFGAGRR
Sbjct: 386 NINGYDIKKGSRVLVNVWTIGRDPKVWDKPDEFFPERFIGNSIDVRGHDYELLPFGAGRR 445
Query: 1438 MCPGYTLGLKVIQSTLANLLHGFSWKLPENMKLEDLNMEEIFGLSTPKRFPLVVVVEPRL 1617
MCPGY LGLKVIQ+TL+NLLHGF W+LP+ K +DLNM+EIFGLSTPK++PLV V EPRL
Sbjct: 446 MCPGYPLGLKVIQATLSNLLHGFKWRLPDGQKKDDLNMDEIFGLSTPKKYPLVAVAEPRL 505
Query: 1618 PAHLYDK 1638
PAH+Y K
Sbjct: 506 PAHVYPK 512
>gb|ADC34701.1| flavonoid 3' hydroxylase [Actinidia chinensis]
Length = 511
Score = 742 bits (1915), Expect = 0.0
Identities = 355/483 (73%), Positives = 412/483 (85%), Gaps = 2/483 (0%)
Frame = +1
Query: 190 RRRCQNLPPGPKPWPIIGNFNLIGSLPHRSIHDLSQKYGPLMHIKFGSRPVVVASSVEMA 369
RRR NLPPGPKPWPIIGN NLIGSLPHRS+HDLSQ+YGP+MH++FGS PVVV SSV A
Sbjct: 28 RRRKLNLPPGPKPWPIIGNLNLIGSLPHRSLHDLSQQYGPIMHLRFGSFPVVVGSSVAAA 87
Query: 370 KQFLKTHDVVFAGRPQTAAGKYTTYNFSDITWSPYGPYWRQARKMCIMELFSSKXXXXXX 549
K FLKT DV FA RP+TAAGK+TTYN+SDITWSPYG YWRQARKMC+ ELFS+K
Sbjct: 88 KTFLKTMDVTFASRPKTAAGKHTTYNYSDITWSPYGAYWRQARKMCLTELFSTKRLESYE 147
Query: 550 XXXXXXTRALLKGLFNSFGNPVSLKDHLSDVSLNVISRIVFGKKYLDEQETSIITPNEFK 729
R+L+K ++ S G P+ +KD+LS VSLNVISR+V G++YLD E+S++TP +FK
Sbjct: 148 YIRIEEMRSLIKEIYQSAGTPIMIKDNLSTVSLNVISRMVLGRRYLDASESSVVTPEDFK 207
Query: 730 KMLDELFLLNGVLNIGDWIPWVGFMDLQGYVKRMKVLAKKFDRFLEHVIDEHNEKRH--G 903
KMLDELFLLNGV NIGD IPW+ F+DLQG VKRMKV+AKKFD+FLEHV+DEHNEKR G
Sbjct: 208 KMLDELFLLNGVFNIGDSIPWIDFLDLQGNVKRMKVVAKKFDKFLEHVLDEHNEKRREEG 267
Query: 904 VKAWVPKDMIDVLLELADDPNLDVKLTRTSVKAFSQDLIAGGTESSAVTVEWAIAELLKH 1083
V +V KDM+DVLL+LADDPNLD+KL R VKAF+QDL+AGGTESSAVTVEWAI+ELLK
Sbjct: 268 V-GFVAKDMVDVLLQLADDPNLDIKLERHGVKAFTQDLLAGGTESSAVTVEWAISELLKK 326
Query: 1084 PKIFQKATEELDRVIGKERWIEEKDMPQLPYIEAIMKETMRLHPVAPMLVPRMTRENLNI 1263
PKIF+ ATEELDRVIGK RW+EEKD+P LPYIEAI+KETMR+HPVAPMLVPR+TRE+ +
Sbjct: 327 PKIFKMATEELDRVIGKSRWVEEKDIPNLPYIEAIVKETMRMHPVAPMLVPRLTREDCQV 386
Query: 1264 AGYDIPAGTGALVSVWTIGRDPTLWEKPEEFYPERFIGKSIDVKGNDFELLPFGAGRRMC 1443
GYDI GT LV+VWTIGRDP LW++P EF PERFIGKSIDVKG DFELLPFG+GRRMC
Sbjct: 387 DGYDIQEGTRVLVNVWTIGRDPELWDEPNEFCPERFIGKSIDVKGQDFELLPFGSGRRMC 446
Query: 1444 PGYTLGLKVIQSTLANLLHGFSWKLPENMKLEDLNMEEIFGLSTPKRFPLVVVVEPRLPA 1623
PGY+LGLKVIQ++LANLLHGF+W LP NMK++DLNM+EIFGLSTP++ PL V EPRLP
Sbjct: 447 PGYSLGLKVIQASLANLLHGFTWNLPANMKVDDLNMDEIFGLSTPRKVPLATVAEPRLPP 506
Query: 1624 HLY 1632
HLY
Sbjct: 507 HLY 509
>ref|XP_002521317.1| flavonoid 3-hydroxylase, putative [Ricinus communis]
gi|223539395|gb|EEF40985.1| flavonoid 3-hydroxylase,
putative [Ricinus communis]
Length = 511
Score = 742 bits (1915), Expect = 0.0
Identities = 356/484 (73%), Positives = 411/484 (84%), Gaps = 2/484 (0%)
Frame = +1
Query: 187 LRRRCQNLPPGPKPWPIIGNFNLIGSLPHRSIHDLSQKYGPLMHIKFGSRPVVVASSVEM 366
LRRR NLPPGPKPWPIIGN NLIG+LPHRS+H LSQ YGP+M +KFGS PVVV SSVEM
Sbjct: 26 LRRRKLNLPPGPKPWPIIGNLNLIGALPHRSLHSLSQTYGPIMQLKFGSFPVVVGSSVEM 85
Query: 367 AKQFLKTHDVVFAGRPQTAAGKYTTYNFSDITWSPYGPYWRQARKMCIMELFSSKXXXXX 546
AK LKTHDV FAGRP+ AAGKYTTYN+SDITWSPYG YWRQARKMC+MELFS+K
Sbjct: 86 AKAILKTHDVAFAGRPKIAAGKYTTYNYSDITWSPYGAYWRQARKMCVMELFSAKRLESY 145
Query: 547 XXXXXXXTRALLKGLFNSFGNPVSLKDHLSDVSLNVISRIVFGKKYLDEQET--SIITPN 720
R LLK +F S GNP++LKDHL+D+SLNVISR+V GKKY + E I+TP
Sbjct: 146 EYIRIEELRLLLKSMFLSSGNPINLKDHLTDLSLNVISRMVLGKKYTVKSENVDEIVTPE 205
Query: 721 EFKKMLDELFLLNGVLNIGDWIPWVGFMDLQGYVKRMKVLAKKFDRFLEHVIDEHNEKRH 900
EFK+MLDELFLLNGVL+IGD I W+ F+DLQGY+KRMK ++KKFDRFLEHV+DEH+ +R
Sbjct: 206 EFKEMLDELFLLNGVLDIGDSISWLAFLDLQGYIKRMKTVSKKFDRFLEHVLDEHDARRK 265
Query: 901 GVKAWVPKDMIDVLLELADDPNLDVKLTRTSVKAFSQDLIAGGTESSAVTVEWAIAELLK 1080
V V KDM+DVLL+LADDPNL++KL R VK F+QDLIAGGTESSAVTVEWAI+ELL+
Sbjct: 266 RVDNHVAKDMVDVLLQLADDPNLEIKLERNGVKGFTQDLIAGGTESSAVTVEWAISELLR 325
Query: 1081 HPKIFQKATEELDRVIGKERWIEEKDMPQLPYIEAIMKETMRLHPVAPMLVPRMTRENLN 1260
P+IF KATEELDRVIG+ERW+EEKD+ LP+I AI+KETMRLHPVAPMLVPR RE+
Sbjct: 326 KPEIFGKATEELDRVIGRERWVEEKDIVNLPFIYAIIKETMRLHPVAPMLVPRQCREDTK 385
Query: 1261 IAGYDIPAGTGALVSVWTIGRDPTLWEKPEEFYPERFIGKSIDVKGNDFELLPFGAGRRM 1440
+AGYDIP GT LV+VWTIGRDP++W+ P+EF PERFIGK+IDVKG DFELLPFGAGRRM
Sbjct: 386 VAGYDIPEGTRVLVNVWTIGRDPSIWDNPDEFCPERFIGKTIDVKGCDFELLPFGAGRRM 445
Query: 1441 CPGYTLGLKVIQSTLANLLHGFSWKLPENMKLEDLNMEEIFGLSTPKRFPLVVVVEPRLP 1620
CPGY LG+KVIQ++LANLLHGF WKLP +MK+EDLNMEEIFGLSTPK+FPLVVV EPRLP
Sbjct: 446 CPGYPLGIKVIQASLANLLHGFKWKLPGDMKIEDLNMEEIFGLSTPKKFPLVVVAEPRLP 505
Query: 1621 AHLY 1632
+H+Y
Sbjct: 506 SHVY 509
>ref|XP_002331194.1| cytochrome P450 [Populus trichocarpa] gi|222873315|gb|EEF10446.1|
cytochrome P450 [Populus trichocarpa]
Length = 511
Score = 737 bits (1902), Expect = 0.0
Identities = 351/488 (71%), Positives = 412/488 (84%), Gaps = 4/488 (0%)
Frame = +1
Query: 187 LRRRCQNLPPGPKPWPIIGNFNLIGSLPHRSIHDLSQKYGPLMHIKFGSRPVVVASSVEM 366
LRRR LPPGPKPWPIIGN NLIG LPHRS+H LSQKYGP+M ++FGS PVVV SSVEM
Sbjct: 24 LRRRKLKLPPGPKPWPIIGNLNLIGELPHRSLHALSQKYGPIMQVQFGSFPVVVGSSVEM 83
Query: 367 AKQFLKTHDVVFAGRPQTAAGKYTTYNFSDITWSPYGPYWRQARKMCIMELFSSKXXXXX 546
AK LKTHDV+F+GRP+TAAGKYTTYN+SDITWSPYGPYWRQARKMC+MELFS+K
Sbjct: 84 AKTILKTHDVIFSGRPKTAAGKYTTYNYSDITWSPYGPYWRQARKMCLMELFSAKRLESY 143
Query: 547 XXXXXXXTRALLKGLFNSFGNPVSLKDHLSDVSLNVISRIVFGKKYL---DEQETSIITP 717
+ALLK L S G P++LKDHL+DVSLNVISR+V GKKY E E I+TP
Sbjct: 144 EYIRVEELKALLKTLHKSSGRPINLKDHLTDVSLNVISRMVLGKKYTVKSSENEKEIVTP 203
Query: 718 NEFKKMLDELFLLNGVLNIGDWIPWVGFMDLQGYVKRMKVLAKKFDRFLEHVIDEHNEKR 897
EFK+MLDELFLLNGVL+IGD IPW+ F+DLQGY+KRMKVL+KKFD+F+EHV+DEH +R
Sbjct: 204 EEFKEMLDELFLLNGVLDIGDSIPWIAFLDLQGYIKRMKVLSKKFDKFMEHVLDEHESRR 263
Query: 898 HGV-KAWVPKDMIDVLLELADDPNLDVKLTRTSVKAFSQDLIAGGTESSAVTVEWAIAEL 1074
+ W PKDM+DVLL+LA DPNL+VKL R VKAFSQDLIAGGTESSAVTVEWAI+E+
Sbjct: 264 KTEDENWEPKDMVDVLLQLASDPNLEVKLERHGVKAFSQDLIAGGTESSAVTVEWAISEI 323
Query: 1075 LKHPKIFQKATEELDRVIGKERWIEEKDMPQLPYIEAIMKETMRLHPVAPMLVPRMTREN 1254
L+ P++F+KA+EELDRVIG+ERW+EEKDM LPYI AI KE MRLHPVAPMLVPR RE+
Sbjct: 324 LRKPEVFEKASEELDRVIGRERWVEEKDMVNLPYIYAIAKEVMRLHPVAPMLVPREARED 383
Query: 1255 LNIAGYDIPAGTGALVSVWTIGRDPTLWEKPEEFYPERFIGKSIDVKGNDFELLPFGAGR 1434
+N+ GYDI G+ LV+VWTIGRDP +W+KP+EF PERFIG SIDV+G+D+ELLPFGAGR
Sbjct: 384 INVNGYDIKKGSRVLVNVWTIGRDPKVWDKPDEFCPERFIGNSIDVRGHDYELLPFGAGR 443
Query: 1435 RMCPGYTLGLKVIQSTLANLLHGFSWKLPENMKLEDLNMEEIFGLSTPKRFPLVVVVEPR 1614
RMCPGY LGLKVIQ+TL+NLLHGF W+LP+ ++ E+L+MEEIFGLSTPK++PLV V EPR
Sbjct: 444 RMCPGYPLGLKVIQATLSNLLHGFKWRLPDGVRKEELSMEEIFGLSTPKKYPLVAVAEPR 503
Query: 1615 LPAHLYDK 1638
LPAH+Y K
Sbjct: 504 LPAHVYPK 511