BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00004139
         (538 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin...   213   1e-53
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin...   199   2e-49
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi...   197   6e-49
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      197   8e-49
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   185   3e-45

>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=EcTNMT gi|226897702|gb|ACO90222.1|
           tetrahydroprotoberberine N-methyltransferase
           [Eschscholzia californica]
          Length = 350

 Score =  213 bits (543), Expect = 1e-53
 Identities = 95/162 (58%), Positives = 126/162 (77%)
 Frame = -1

Query: 535 YDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAEKFEALDSDDWYSEYL 356
           YDR++++E +EHMKNIQLF+ K+S WM +D+LLFVDH+ H  F   FEALD DDWYS ++
Sbjct: 189 YDRILVVETIEHMKNIQLFMKKLSTWMTEDSLLFVDHISHKTFNHNFEALDEDDWYSGFI 248

Query: 355 FLKGSVTVIAASTLLYFQDDVSVLNHWILNGKHMARTHESWLKKLLNNRNAARDILKVCL 176
           F KG VT++++STLLYFQDDVS L+HW++NG HMAR+ E+W KKL     AAR+IL+  L
Sbjct: 249 FPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDETIEAAREILEPGL 308

Query: 175 GTEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 50
           G++EA  + I H RTF +   EQ+S NNGEEWMI+ +LF+KK
Sbjct: 309 GSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQILFKKK 350


>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
           Short=PsTNMT gi|67764091|gb|AAY79177.1|
           S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
           cis-N-methyltransferase [Papaver somniferum]
          Length = 358

 Score =  199 bits (507), Expect = 2e-49
 Identities = 90/163 (55%), Positives = 123/163 (75%), Gaps = 1/163 (0%)
 Frame = -1

Query: 535 YDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAEKFEALDSDDWYSEYL 356
           YDR+++IEA+EHMKN+QLF+ K+S WM K++LLFVDHVCH  FA  FEA+D DDWYS ++
Sbjct: 196 YDRLLMIEAIEHMKNLQLFMKKLSTWMTKESLLFVDHVCHKTFAHFFEAVDEDDWYSGFI 255

Query: 355 FLKGSVTVIAASTLLYFQDDVSVLNHWILNGKHMARTHESWLKKLLNNRNAARDILKVCL 176
           F  G  T++AA++LLYFQDDVSV++HW++NG HMAR+ + W K L  N  AA++IL   L
Sbjct: 256 FPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGL 315

Query: 175 -GTEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 50
            G+ E     + H RTF +   EQ+S NNG+EWM++ +LF+KK
Sbjct: 316 GGSHETVNGVVTHIRTFCMGGYEQFSMNNGDEWMVAQLLFKKK 358


>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
           Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
           tetrahydroprotoberberine N-methyltransferase [Papaver
           bracteatum]
          Length = 358

 Score =  197 bits (502), Expect = 6e-49
 Identities = 89/163 (54%), Positives = 124/163 (76%), Gaps = 1/163 (0%)
 Frame = -1

Query: 535 YDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAEKFEALDSDDWYSEYL 356
           YDR+++IEA+EHMKN+QLF+ K+S WM +++LLFVDHVCH  FA  FEA+D DDWYS ++
Sbjct: 196 YDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHKTFAHFFEAVDEDDWYSGFI 255

Query: 355 FLKGSVTVIAASTLLYFQDDVSVLNHWILNGKHMARTHESWLKKLLNNRNAARDILKVCL 176
           F  G  T++AA++LLYFQDDVSV++HW++NG HMAR+ + W K L  N  AA++IL   L
Sbjct: 256 FPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGL 315

Query: 175 -GTEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 50
            G+ EA    + H RTF +   EQ+S N+G+EWM++ +LF+KK
Sbjct: 316 GGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFKKK 358


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  197 bits (501), Expect = 8e-49
 Identities = 87/162 (53%), Positives = 119/162 (73%)
 Frame = -1

Query: 535 YDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAEKFEALDSDDWYSEYL 356
           YDR+++IE  EHMKN +L L KIS+W+ KD LLF++H+CH  FA  +E LD DDW++EY+
Sbjct: 197 YDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYV 256

Query: 355 FLKGSVTVIAASTLLYFQDDVSVLNHWILNGKHMARTHESWLKKLLNNRNAARDILKVCL 176
           F  G++ + +AS  LYFQDDVSV+NHW L+GKH +RT+E WLK+L  N +  + + +  +
Sbjct: 257 FPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLM 316

Query: 175 GTEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 50
           G EE A K IN++R F L+  E +  NNGEEWM SHVLF+KK
Sbjct: 317 GNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 358


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
           gi|51980204|gb|AAU20766.1| (S)-coclaurine
           N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  185 bits (470), Expect = 3e-45
 Identities = 84/163 (51%), Positives = 114/163 (69%), Gaps = 1/163 (0%)
 Frame = -1

Query: 535 YDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAEKFEALDSDDWYSEYL 356
           YDR++++E  EHMKN +L L KI +WM KD LLFV+H+CH  FA  +E +D DDW++EY+
Sbjct: 199 YDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDEDDWFTEYV 258

Query: 355 FLKGSVTVIAASTLLYFQDDVSVLNHWILNGKHMARTHESWLKKLLNNRNAARDILKVCL 176
           F  G++ + +AS  LYFQDDVSV+NHW L+GKH +RT+E WLK+L  N    + +     
Sbjct: 259 FPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVELIKPMFVTIT 318

Query: 175 G-TEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 50
           G   + A K IN++R F L+  E +  NNGEEWM SHVLF+KK
Sbjct: 319 GQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 361