BLASTX nr result
ID: Coptis25_contig00004135
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00004135
(2260 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABR16195.1| unknown [Picea sitchensis] 310 e-137
ref|XP_002510329.1| multidrug resistance pump, putative [Ricinus... 315 e-135
ref|XP_002281913.1| PREDICTED: protein TRANSPARENT TESTA 12 [Vit... 311 e-133
emb|CAN79163.1| hypothetical protein VITISV_019245 [Vitis vinifera] 311 e-133
ref|XP_003550360.1| PREDICTED: protein TRANSPARENT TESTA 12-like... 310 e-133
>gb|ABR16195.1| unknown [Picea sitchensis]
Length = 500
Score = 310 bits (795), Expect(3) = e-137
Identities = 157/272 (57%), Positives = 198/272 (72%), Gaps = 1/272 (0%)
Frame = +3
Query: 813 SIWMIPQLVAYAFNFPLQKFLQAQTKVMAMAWISFGVLVSHVGLSWLCIVKLDMGLLGAA 992
++WM+PQL AYA NFPL KFLQAQ+KVM M+ I+ LV HV LSW+ I K+ GL AA
Sbjct: 177 AVWMLPQLFAYAMNFPLAKFLQAQSKVMVMSIIAGCALVVHVLLSWILIFKVGWGLPAAA 236
Query: 993 ITLDFSWWVLVTGQLIYVFT-CCKDAWTGFSWLAFADLSWFTGLSLASGVMLCLEYWCYM 1169
+ L+ SWW++V Q+ YVF+ C+DAW GFSW AF++L F LSLASGVMLCLE W
Sbjct: 237 LVLNLSWWIIVLAQIGYVFSGACRDAWAGFSWSAFSNLGAFVRLSLASGVMLCLEAWYLY 296
Query: 1170 VLILLAGLLKNPEIQVDAASICMGVEGWLFMIPLAFIAASSVRVSNELGAGHPEAAKFSV 1349
+LILLAG LKNP I VDA SICM + GW FMI + F A SVRVSNELGAG P AAKFSV
Sbjct: 297 LLILLAGYLKNPTIAVDAISICMNLMGWEFMIAIGFNIAISVRVSNELGAGRPRAAKFSV 356
Query: 1350 GVMVSMSLVIETTFVVIILATRNKFPSLFTENKLVMAEASKLAIFLSMSIILCSVQPVLS 1529
V+V + ++ + II TRN+F FT++K+VM+ ++LA L+ +++L SVQPVLS
Sbjct: 357 IVVVGTTFLVGVVLMAIIFITRNEFAVAFTDSKVVMSAVAQLAPLLAFTMLLNSVQPVLS 416
Query: 1530 GVAVGAGWQATVAYVNIVSYYLVGLTTGAILG 1625
GVA+GAGWQA VAY+N+ YYL G+ G +LG
Sbjct: 417 GVAIGAGWQALVAYINLACYYLFGVPLGCLLG 448
Score = 164 bits (414), Expect(3) = e-137
Identities = 77/137 (56%), Positives = 101/137 (73%)
Frame = +1
Query: 331 AREVWKEVKITWSIAGPAILTSLCQYSLGFVTQTLVGHIGTIELAAVGLQGLVVSXXXXX 510
A W+E K W ++GPAI TS+CQYSLG +TQT GHIG +ELAAV ++ V++
Sbjct: 47 ANGCWEESKKLWYLSGPAIFTSVCQYSLGALTQTFAGHIGELELAAVSIENSVIAGLSFG 106
Query: 511 XXXXXXSALETLCGQAYGAGRPGMLGIYMQKSWVILLSTAIPLTLVYIFAAPILKLLGQS 690
SALETLCGQ+ GA R +LG+YMQ+SW+ILLSTA+ ++L+YIFA PIL L+GQ
Sbjct: 107 IMMGMGSALETLCGQSVGARRLDLLGLYMQRSWIILLSTALIMSLIYIFATPILVLIGQQ 166
Query: 691 HEIADTAGKFSIWMIPQ 741
+IAD AGKF++WM+PQ
Sbjct: 167 KDIADMAGKFAVWMLPQ 183
Score = 64.3 bits (155), Expect(3) = e-137
Identities = 27/55 (49%), Positives = 39/55 (70%)
Frame = +2
Query: 1715 ILGFKFHFGLQGLWSGVILGIALQTVILVVMTLRTDWEKEAALAKVRISTWESST 1879
+LG+ F G++G+W+G+I G LQT+IL ++T RT W KEA AK RI+ W +T
Sbjct: 446 LLGYYFDLGVEGIWAGMISGTLLQTIILCIITYRTKWNKEANQAKARITLWGGAT 500
>ref|XP_002510329.1| multidrug resistance pump, putative [Ricinus communis]
gi|223551030|gb|EEF52516.1| multidrug resistance pump,
putative [Ricinus communis]
Length = 483
Score = 315 bits (808), Expect(3) = e-135
Identities = 159/272 (58%), Positives = 199/272 (73%), Gaps = 1/272 (0%)
Frame = +3
Query: 813 SIWMIPQLVAYAFNFPLQKFLQAQTKVMAMAWISFGVLVSHVGLSWLCIVKLDMGLLGAA 992
+IWM+PQL AYA NFP+QKFLQ+Q+KV MAWIS VLV H SWL I+KL GL GAA
Sbjct: 157 AIWMLPQLFAYAVNFPIQKFLQSQSKVYVMAWISAAVLVLHAIFSWLLILKLGWGLTGAA 216
Query: 993 ITLDFSWWVLVTGQLIYVFTCCKD-AWTGFSWLAFADLSWFTGLSLASGVMLCLEYWCYM 1169
ITL+ SWW++V QL+Y+F D AW+GF+WLAFADL F LSLAS VMLCLE+W M
Sbjct: 217 ITLNTSWWIIVIAQLLYIFITKSDGAWSGFTWLAFADLGGFVKLSLASAVMLCLEFWYLM 276
Query: 1170 VLILLAGLLKNPEIQVDAASICMGVEGWLFMIPLAFIAASSVRVSNELGAGHPEAAKFSV 1349
+L+++ G L NP I VDA SICM ++GW MI L F AA SVRVSNELGAG+ AK+SV
Sbjct: 277 ILVVITGRLPNPLIPVDAISICMNLQGWDAMIALGFNAAISVRVSNELGAGNSRRAKYSV 336
Query: 1350 GVMVSMSLVIETTFVVIILATRNKFPSLFTENKLVMAEASKLAIFLSMSIILCSVQPVLS 1529
V+ S+ I + ++ ATR+ FP LFT + V E +KLA+ L ++++L S+QPVLS
Sbjct: 337 KVVSVTSISIGVVCMALVFATRDYFPYLFTTSDAVAKETTKLAVLLGITVLLNSLQPVLS 396
Query: 1530 GVAVGAGWQATVAYVNIVSYYLVGLTTGAILG 1625
GVAVGAGWQ+ VAY+NI YY+VGL G +LG
Sbjct: 397 GVAVGAGWQSLVAYINIGCYYVVGLPAGILLG 428
Score = 153 bits (386), Expect(3) = e-135
Identities = 72/136 (52%), Positives = 100/136 (73%)
Frame = +1
Query: 334 REVWKEVKITWSIAGPAILTSLCQYSLGFVTQTLVGHIGTIELAAVGLQGLVVSXXXXXX 513
+E E K W +AGPAI TS+CQYSLG +TQT G +G ++LAAV ++ V++
Sbjct: 28 KEFSDESKRLWKLAGPAIFTSICQYSLGALTQTFAGLVGEVDLAAVSVENSVIAGLAFGV 87
Query: 514 XXXXXSALETLCGQAYGAGRPGMLGIYMQKSWVILLSTAIPLTLVYIFAAPILKLLGQSH 693
SALETLCGQAYGAG+ MLG+YMQ+SWVILL+TA L +Y+++ PIL+L+G++
Sbjct: 88 MLGMGSALETLCGQAYGAGQLRMLGVYMQRSWVILLTTACLLVPIYVWSPPILELIGETT 147
Query: 694 EIADTAGKFSIWMIPQ 741
+I++ AGKF+IWM+PQ
Sbjct: 148 QISEAAGKFAIWMLPQ 163
Score = 62.8 bits (151), Expect(3) = e-135
Identities = 27/51 (52%), Positives = 37/51 (72%)
Frame = +2
Query: 1715 ILGFKFHFGLQGLWSGVILGIALQTVILVVMTLRTDWEKEAALAKVRISTW 1867
+LGF F FG G+WSG+I GI LQT+IL+++T T+W KEA A+ R+ W
Sbjct: 426 LLGFTFGFGAMGVWSGMIGGICLQTIILIIVTSITNWNKEAEEAESRVKKW 476
>ref|XP_002281913.1| PREDICTED: protein TRANSPARENT TESTA 12 [Vitis vinifera]
gi|302142423|emb|CBI19626.3| unnamed protein product
[Vitis vinifera]
Length = 479
Score = 311 bits (797), Expect(3) = e-133
Identities = 160/272 (58%), Positives = 197/272 (72%), Gaps = 1/272 (0%)
Frame = +3
Query: 813 SIWMIPQLVAYAFNFPLQKFLQAQTKVMAMAWISFGVLVSHVGLSWLCIVKLDMGLLGAA 992
++WMIPQL AYA NFP+QKFLQAQ KV MAWIS VLV H LSWL I+KL GL+GAA
Sbjct: 153 ALWMIPQLFAYALNFPIQKFLQAQRKVFVMAWISVVVLVIHAVLSWLLILKLGYGLVGAA 212
Query: 993 ITLDFSWWVLVTGQLIYVFTCCKD-AWTGFSWLAFADLSWFTGLSLASGVMLCLEYWCYM 1169
+ L+ SWW++V QL+Y+F D AW+GFS LAFADL F LSLAS VMLCLE+W M
Sbjct: 213 VMLNLSWWLIVISQLLYIFITTSDGAWSGFSMLAFADLYGFVKLSLASAVMLCLEFWYLM 272
Query: 1170 VLILLAGLLKNPEIQVDAASICMGVEGWLFMIPLAFIAASSVRVSNELGAGHPEAAKFSV 1349
+L+++ G LKNP I VDA SICM ++GW MI L F AA SVRVSNELGAG+ +AKF+V
Sbjct: 273 ILVVITGRLKNPLIPVDAISICMNIQGWDAMIALGFNAAISVRVSNELGAGNAPSAKFAV 332
Query: 1350 GVMVSMSLVIETTFVVIILATRNKFPSLFTENKLVMAEASKLAIFLSMSIILCSVQPVLS 1529
V+ S+ I + ++ ATR+ FP LFT + V +KLA L ++++L S+QPVLS
Sbjct: 333 VVVSITSMTIGVICMAVVFATRDVFPYLFTTSTAVADLTTKLATLLGVTVLLNSLQPVLS 392
Query: 1530 GVAVGAGWQATVAYVNIVSYYLVGLTTGAILG 1625
GVAVGAGWQ VAY+NI YYLVGL G +LG
Sbjct: 393 GVAVGAGWQHIVAYINIGCYYLVGLPAGLLLG 424
Score = 154 bits (388), Expect(3) = e-133
Identities = 74/136 (54%), Positives = 99/136 (72%)
Frame = +1
Query: 334 REVWKEVKITWSIAGPAILTSLCQYSLGFVTQTLVGHIGTIELAAVGLQGLVVSXXXXXX 513
R+ E K W +AGPAI T++CQYSLG +TQT G +G ELAAV ++ V++
Sbjct: 24 RKFCAESKKLWKLAGPAIFTAICQYSLGALTQTFAGLVGETELAAVSVENSVIAGLAFGV 83
Query: 514 XXXXXSALETLCGQAYGAGRPGMLGIYMQKSWVILLSTAIPLTLVYIFAAPILKLLGQSH 693
SALETLCGQAYGAG+ MLGIYMQ+SWVILL+TA L +Y+FA PIL+L+G++
Sbjct: 84 MLGMGSALETLCGQAYGAGKIRMLGIYMQRSWVILLTTACCLVPIYVFAPPILELIGETT 143
Query: 694 EIADTAGKFSIWMIPQ 741
+I++ AG+F++WMIPQ
Sbjct: 144 QISEAAGQFALWMIPQ 159
Score = 61.2 bits (147), Expect(3) = e-133
Identities = 28/54 (51%), Positives = 37/54 (68%)
Frame = +2
Query: 1715 ILGFKFHFGLQGLWSGVILGIALQTVILVVMTLRTDWEKEAALAKVRISTWESS 1876
+LGFKF G +G+W G+I GI LQT+ILV +T T+W KEA A+ R+ W S
Sbjct: 422 LLGFKFGLGAEGIWGGMIGGICLQTIILVGITSWTNWNKEAEEAESRVKKWGGS 475
>emb|CAN79163.1| hypothetical protein VITISV_019245 [Vitis vinifera]
Length = 459
Score = 311 bits (797), Expect(3) = e-133
Identities = 160/272 (58%), Positives = 197/272 (72%), Gaps = 1/272 (0%)
Frame = +3
Query: 813 SIWMIPQLVAYAFNFPLQKFLQAQTKVMAMAWISFGVLVSHVGLSWLCIVKLDMGLLGAA 992
++WMIPQL AYA NFP+QKFLQAQ KV MAWIS VLV H LSWL I+KL GL+GAA
Sbjct: 133 ALWMIPQLFAYALNFPIQKFLQAQRKVFVMAWISVVVLVIHAVLSWLLILKLGYGLVGAA 192
Query: 993 ITLDFSWWVLVTGQLIYVFTCCKD-AWTGFSWLAFADLSWFTGLSLASGVMLCLEYWCYM 1169
+ L+ SWW++V QL+Y+F D AW+GFS LAFADL F LSLAS VMLCLE+W M
Sbjct: 193 VMLNLSWWLIVISQLLYIFITTSDGAWSGFSMLAFADLYGFVKLSLASAVMLCLEFWYLM 252
Query: 1170 VLILLAGLLKNPEIQVDAASICMGVEGWLFMIPLAFIAASSVRVSNELGAGHPEAAKFSV 1349
+L+++ G LKNP I VDA SICM ++GW MI L F AA SVRVSNELGAG+ +AKF+V
Sbjct: 253 ILVVITGRLKNPLIPVDAISICMNIQGWDAMIALGFNAAISVRVSNELGAGNAPSAKFAV 312
Query: 1350 GVMVSMSLVIETTFVVIILATRNKFPSLFTENKLVMAEASKLAIFLSMSIILCSVQPVLS 1529
V+ S+ I + ++ ATR+ FP LFT + V +KLA L ++++L S+QPVLS
Sbjct: 313 VVVSITSMTIGVICMAVVFATRDVFPYLFTTSTAVADLTTKLATLLGVTVLLNSLQPVLS 372
Query: 1530 GVAVGAGWQATVAYVNIVSYYLVGLTTGAILG 1625
GVAVGAGWQ VAY+NI YYLVGL G +LG
Sbjct: 373 GVAVGAGWQHIVAYINIGCYYLVGLPAGLLLG 404
Score = 153 bits (386), Expect(3) = e-133
Identities = 73/131 (55%), Positives = 97/131 (74%)
Frame = +1
Query: 349 EVKITWSIAGPAILTSLCQYSLGFVTQTLVGHIGTIELAAVGLQGLVVSXXXXXXXXXXX 528
E K W +AGPAI T++CQYSLG +TQT G +G ELAAV ++ V++
Sbjct: 9 ESKKLWKLAGPAIFTAICQYSLGALTQTFAGLVGETELAAVSVENSVIAGLAFGVMLGMG 68
Query: 529 SALETLCGQAYGAGRPGMLGIYMQKSWVILLSTAIPLTLVYIFAAPILKLLGQSHEIADT 708
SALETLCGQAYGAG+ MLGIYMQ+SWVILL+TA L +Y+FA PIL+L+G++ +I++
Sbjct: 69 SALETLCGQAYGAGKIRMLGIYMQRSWVILLTTACCLVPIYVFAPPILELIGETTQISEA 128
Query: 709 AGKFSIWMIPQ 741
AG+F++WMIPQ
Sbjct: 129 AGQFALWMIPQ 139
Score = 61.2 bits (147), Expect(3) = e-133
Identities = 28/54 (51%), Positives = 37/54 (68%)
Frame = +2
Query: 1715 ILGFKFHFGLQGLWSGVILGIALQTVILVVMTLRTDWEKEAALAKVRISTWESS 1876
+LGFKF G +G+W G+I GI LQT+ILV +T T+W KEA A+ R+ W S
Sbjct: 402 LLGFKFGLGAEGIWGGMIGGICLQTIILVGITSWTNWNKEAEEAESRVKKWGGS 455
>ref|XP_003550360.1| PREDICTED: protein TRANSPARENT TESTA 12-like isoform 2 [Glycine max]
Length = 487
Score = 310 bits (793), Expect(3) = e-133
Identities = 158/272 (58%), Positives = 199/272 (73%), Gaps = 1/272 (0%)
Frame = +3
Query: 813 SIWMIPQLVAYAFNFPLQKFLQAQTKVMAMAWISFGVLVSHVGLSWLCIVKLDMGLLGAA 992
++WMIPQL AYA NFP+ KFLQAQ KV+ M WIS VLV H SWL I KL GL+GAA
Sbjct: 156 AVWMIPQLFAYAINFPIVKFLQAQRKVLVMLWISVVVLVLHTFFSWLVIFKLGWGLIGAA 215
Query: 993 ITLDFSWWVLVTGQLIYVFTCCKD-AWTGFSWLAFADLSWFTGLSLASGVMLCLEYWCYM 1169
+TL+ SWWV+V QL+Y+F D AW+GF+WLAF+DL F LSLAS VMLCLE+W M
Sbjct: 216 VTLNTSWWVIVIAQLLYIFITKSDGAWSGFTWLAFSDLFGFVKLSLASAVMLCLEFWYLM 275
Query: 1170 VLILLAGLLKNPEIQVDAASICMGVEGWLFMIPLAFIAASSVRVSNELGAGHPEAAKFSV 1349
+L+++ G L+NP I VDA SICM + GW MI + F AA SVRVSNELGAG +AAKFSV
Sbjct: 276 ILVVITGRLENPLIPVDAISICMNINGWDAMIAIGFNAAISVRVSNELGAGDFKAAKFSV 335
Query: 1350 GVMVSMSLVIETTFVVIILATRNKFPSLFTENKLVMAEASKLAIFLSMSIILCSVQPVLS 1529
V+ S+ I ++ +L T++ FP LFT + V E ++L+ L+++++L S+QPVLS
Sbjct: 336 WVVSITSVSIGVVVMIGVLLTKDYFPYLFTTSVPVANETTRLSALLAVTVLLNSLQPVLS 395
Query: 1530 GVAVGAGWQATVAYVNIVSYYLVGLTTGAILG 1625
GVAVGAGWQ+ VAY+NIV YYLVGL G ILG
Sbjct: 396 GVAVGAGWQSLVAYINIVCYYLVGLPAGIILG 427
Score = 154 bits (389), Expect(3) = e-133
Identities = 75/131 (57%), Positives = 97/131 (74%)
Frame = +1
Query: 349 EVKITWSIAGPAILTSLCQYSLGFVTQTLVGHIGTIELAAVGLQGLVVSXXXXXXXXXXX 528
E K W IAGPAI+TS+CQYSLG +TQT G +G ++LAAV ++ V++
Sbjct: 32 ESKKLWKIAGPAIVTSICQYSLGALTQTFAGLVGDLDLAAVSVENSVIAGLAFGVMLGMG 91
Query: 529 SALETLCGQAYGAGRPGMLGIYMQKSWVILLSTAIPLTLVYIFAAPILKLLGQSHEIADT 708
SALETLCGQAYGAG+ MLG+YMQ+SWVIL TA+ L +YI++ PIL+L GQ+ EI+D
Sbjct: 92 SALETLCGQAYGAGQIRMLGVYMQRSWVILFITALILLPLYIWSPPILRLAGQTAEISDA 151
Query: 709 AGKFSIWMIPQ 741
AGKF++WMIPQ
Sbjct: 152 AGKFAVWMIPQ 162
Score = 61.2 bits (147), Expect(3) = e-133
Identities = 27/57 (47%), Positives = 38/57 (66%)
Frame = +2
Query: 1715 ILGFKFHFGLQGLWSGVILGIALQTVILVVMTLRTDWEKEAALAKVRISTWESSTDK 1885
ILGFK G +G+WSG+I GI LQT IL+++T +W+KEA A+ R+ W + K
Sbjct: 425 ILGFKLGLGAEGIWSGMIAGIVLQTTILIIVTSIRNWKKEAEEAESRVRKWGGAISK 481