BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00003857
         (1758 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285593.1| PREDICTED: aspartic proteinase nepenthesin-2...   530   e-148
ref|XP_002324349.1| predicted protein [Populus trichocarpa] gi|2...   508   e-141
ref|XP_002283470.1| PREDICTED: aspartic proteinase nepenthesin-2...   506   e-140
ref|XP_003532146.1| PREDICTED: aspartic proteinase nepenthesin-1...   499   e-139
ref|XP_002515388.1| Aspartic proteinase nepenthesin-2 precursor,...   499   e-139

>ref|XP_002285593.1| PREDICTED: aspartic proteinase nepenthesin-2 [Vitis vinifera]
          Length = 481

 Score =  530 bits (1365), Expect = e-148
 Identities = 265/500 (53%), Positives = 348/500 (69%), Gaps = 1/500 (0%)
 Frame = -2

Query: 1697 MAVVVPSV-LLQFLVYTSFIVFVSLLCFGEKSFAYGRDEVSESIHQSTVHVIVVSTILPP 1521
            MA  + ++ LL+FL+Y++ +     L F  +  A            ST+H + +++++P 
Sbjct: 1    MATPISTIFLLKFLLYSALLSSKRGLAFQGRKTALSTP--------STLHNVHITSLMPS 52

Query: 1520 KSCSSFTSFKDRRSIPLLKIVHRHSPCSGSFHPEGQKKRATTNQILSTEQILHQDQIRVE 1341
              CS      D+R+   L+++H+H PCS     +G+    T        Q+L QD+ RV 
Sbjct: 53   SVCSPSPKGDDKRAS--LEVIHKHGPCSKLSQDKGRSPSRT--------QMLDQDESRVN 102

Query: 1340 SLHYKLSNKSKMSQEQSSKATVLPAKSGVSLGTGNYIVRVGFGTPKKYLSLMFDTGSDLT 1161
            S+  +L+       +       LP+KSG ++GTGNY+V VG GTPK+ L+ +FDTGSDLT
Sbjct: 103  SIRSRLAKNPADGGKLKGSKVTLPSKSGSTIGTGNYVVTVGLGTPKRDLTFIFDTGSDLT 162

Query: 1160 WIQCKPCVRYCHEQQESIFNPSESTSYSNITCNTSECSQLKSATGNTPACGSSSTCIYGI 981
            W QC+PC RYC+ QQE IFNPS+STSY+NI+C++  C +LKS TGN+P+C S+STC+YGI
Sbjct: 163  WTQCEPCARYCYHQQEPIFNPSKSTSYTNISCSSPTCDELKSGTGNSPSC-SASTCVYGI 221

Query: 980  QYGDQSFSVGYFGRETLTLTPRDVFNNFRFGCGQNNRGIFGKTAGLLGLGRNELSFVSQT 801
            QYGDQS+SVG+F ++ L LT  DVFNNF FGCGQNNRG+F   AGL+GLGRN LS VSQT
Sbjct: 222  QYGDQSYSVGFFAQDKLALTSTDVFNNFLFGCGQNNRGLFVGVAGLIGLGRNALSLVSQT 281

Query: 800  SQKYGGRFSYCLPSSSSSVGYLAFGVDRSVSSTVKFTPMVIDSRGPSFYFLEIIEINVGD 621
            +QKYG  FSYCLPS+SSS GYL FG     S  VKFTP +++S+GPSFYFL +I I+VG 
Sbjct: 282  AQKYGKLFSYCLPSTSSSTGYLTFGSGGGTSKAVKFTPSLVNSQGPSFYFLNLIAISVGG 341

Query: 620  RKLEISASVFSFSGMIIDSGTVITRLPKTAYTSLQSAFQQLMSSYPSAKPLSILDTCYNF 441
            RKL  SASVFS +G IIDSGTVI+RLP TAY+ L+++FQQ MS YP A P SILDTCY+F
Sbjct: 342  RKLSTSASVFSTAGTIIDSGTVISRLPPTAYSDLRASFQQQMSKYPKAAPASILDTCYDF 401

Query: 440  TDYDEVNIPKISMLFKGGVTLDLDPSGILIGASASQVCLAFAGNQDDKDVGILGNKQQQK 261
            + YD V++PKI++ F  G  +DLDPSGI    + SQVCLAFAGN D  D+ ILGN QQ+ 
Sbjct: 402  SQYDTVDVPKINLYFSDGAEMDLDPSGIFYILNISQVCLAFAGNSDATDIAILGNVQQKT 461

Query: 260  LEVIYDVARSRLGFVPGICK 201
             +V+YDVA  R+GF PG C+
Sbjct: 462  FDVVYDVAGGRIGFAPGGCE 481


>ref|XP_002324349.1| predicted protein [Populus trichocarpa] gi|222865783|gb|EEF02914.1|
            predicted protein [Populus trichocarpa]
          Length = 490

 Score =  508 bits (1309), Expect = e-141
 Identities = 273/503 (54%), Positives = 346/503 (68%), Gaps = 5/503 (0%)
 Frame = -2

Query: 1697 MAVVVPSVLLQFLVYT-SFIVFVSLLCFGEKSFAYGRDEVSESIHQSTVHVIVVSTILPP 1521
            MA  + SV L    +  ++ + + LL   EK +A    +V+ES H    H I VS++LP 
Sbjct: 1    MATPISSVSLTMRCFLYAYFLCLCLLFSLEKGYALEGRKVAESHHS---HSIEVSSLLPS 57

Query: 1520 KSCSSFTS-FKDRRSIPLLKIVHRHSPCSGSFHPEGQKKRATTNQILSTEQILHQDQIRV 1344
             SC   T    +  +   LK+VH+H PCS     E       T       +IL QDQ RV
Sbjct: 58   ASCKPSTKVLSNNDNKASLKVVHKHGPCSKLSQDEASAAPTHT-------EILLQDQSRV 110

Query: 1343 ESLHYKLSNKSKMSQEQSSK---ATVLPAKSGVSLGTGNYIVRVGFGTPKKYLSLMFDTG 1173
            +S+H +LSN SK S  +  K   +T +PAK G ++G+GNYIV VG GTPKK LSL+FDTG
Sbjct: 111  KSIHSRLSN-SKTSGGKDVKVTDSTTIPAKDGSTVGSGNYIVTVGLGTPKKDLSLIFDTG 169

Query: 1172 SDLTWIQCKPCVRYCHEQQESIFNPSESTSYSNITCNTSECSQLKSATGNTPACGSSSTC 993
            SD+TW QC+PC R C++Q+E IF+PS+STSY+NI+C++S C+ L SATGNTP C SS+ C
Sbjct: 170  SDITWTQCQPCARSCYKQKEQIFDPSQSTSYTNISCSSSICNSLTSATGNTPGCASSA-C 228

Query: 992  IYGIQYGDQSFSVGYFGRETLTLTPRDVFNNFRFGCGQNNRGIFGKTAGLLGLGRNELSF 813
            +YGIQYGD SFSVG+FG E LTLT  D FNN  FGCGQNN+G+FG +AGLLGLGR++LS 
Sbjct: 229  VYGIQYGDSSFSVGFFGTEKLTLTSTDAFNNIYFGCGQNNQGLFGGSAGLLGLGRDKLSV 288

Query: 812  VSQTSQKYGGRFSYCLPSSSSSVGYLAFGVDRSVSSTVKFTPMVIDSRGPSFYFLEIIEI 633
            VSQT+QKY   FSYCLPSSSSS G+L FG   S S   KFTP+   S GPSFY L+   I
Sbjct: 289  VSQTAQKYNKIFSYCLPSSSSSTGFLTFG--GSASKNAKFTPLSTISAGPSFYGLDFTGI 346

Query: 632  NVGDRKLEISASVFSFSGMIIDSGTVITRLPKTAYTSLQSAFQQLMSSYPSAKPLSILDT 453
            +VG +KL ISASVFS +G IIDSGTVITRLP  AY++L+++F+ LMS YP  K LSILDT
Sbjct: 347  SVGGKKLAISASVFSTAGAIIDSGTVITRLPPAAYSALRASFRNLMSKYPMTKALSILDT 406

Query: 452  CYNFTDYDEVNIPKISMLFKGGVTLDLDPSGILIGASASQVCLAFAGNQDDKDVGILGNK 273
            CY+F+ Y  +++PKI   F  G+ +D+D +GIL  +S SQVCLAFAGN D  DV I GN 
Sbjct: 407  CYDFSSYTTISVPKIGFSFSSGIEVDIDATGILYASSLSQVCLAFAGNSDATDVFIFGNV 466

Query: 272  QQQKLEVIYDVARSRLGFVPGIC 204
            QQ+ LEV YD +  ++GF PG C
Sbjct: 467  QQKTLEVFYDGSAGKVGFAPGGC 489


>ref|XP_002283470.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Vitis vinifera]
          Length = 490

 Score =  506 bits (1302), Expect = e-140
 Identities = 266/501 (53%), Positives = 348/501 (69%), Gaps = 3/501 (0%)
 Frame = -2

Query: 1697 MAVVVPSV-LLQFLVYTSFIVFVSLLCFGEKSFAYGRDEVSESIHQSTVHVIV-VSTILP 1524
            MA  + ++ LL+FL+Y S +   S        FA    E +ES H   +H  V +++++P
Sbjct: 8    MATPISTICLLRFLLYASLLSLKS-------GFAIEGRESAESHHVQPIHHNVHITSLMP 60

Query: 1523 PKSCSSFTSFKDRRSIPLLKIVHRHSPCSGSFHPEGQKKRATTNQILSTEQILHQDQIRV 1344
              +CS      D+R+   L++VH+H PCS        K R       S  QIL QD+ RV
Sbjct: 61   SSACSPSPKGHDQRAS--LEVVHKHGPCS--------KLRPHKANSPSHTQILAQDESRV 110

Query: 1343 ESLHYKLS-NKSKMSQEQSSKATVLPAKSGVSLGTGNYIVRVGFGTPKKYLSLMFDTGSD 1167
             S+  +L+ N +  S  ++SKAT LP+KS  +LG+GNY+V VG G+PK+ L+ +FDTGSD
Sbjct: 111  ASIQSRLAKNLAGGSNLKASKAT-LPSKSASTLGSGNYVVTVGLGSPKRDLTFIFDTGSD 169

Query: 1166 LTWIQCKPCVRYCHEQQESIFNPSESTSYSNITCNTSECSQLKSATGNTPACGSSSTCIY 987
            LTW QC+PCV YC++Q+E IF+PS S SYSN++C++  C +L+SATGN+P C SSSTC+Y
Sbjct: 170  LTWTQCEPCVGYCYQQREHIFDPSTSLSYSNVSCDSPSCEKLESATGNSPGC-SSSTCLY 228

Query: 986  GIQYGDQSFSVGYFGRETLTLTPRDVFNNFRFGCGQNNRGIFGKTAGLLGLGRNELSFVS 807
            GI+YGD S+S+G+F RE L+LT  DVFNNF+FGCGQNNRG+FG TAGLLGL RN LS VS
Sbjct: 229  GIRYGDGSYSIGFFAREKLSLTSTDVFNNFQFGCGQNNRGLFGGTAGLLGLARNPLSLVS 288

Query: 806  QTSQKYGGRFSYCLPSSSSSVGYLAFGVDRSVSSTVKFTPMVIDSRGPSFYFLEIIEINV 627
            QT+QKYG  FSYCLPSSSSS GYL+FG     S  VKFTP  ++S  PSFYFL+++ I+V
Sbjct: 289  QTAQKYGKVFSYCLPSSSSSTGYLSFGSGDGDSKAVKFTPSEVNSDYPSFYFLDMVGISV 348

Query: 626  GDRKLEISASVFSFSGMIIDSGTVITRLPKTAYTSLQSAFQQLMSSYPSAKPLSILDTCY 447
            G+RKL I  SVFS +G IIDSGTVI+RLP T Y+S+Q  F++LMS YP  K +SILDTCY
Sbjct: 349  GERKLPIPKSVFSTAGTIIDSGTVISRLPPTVYSSVQKVFRELMSDYPRVKGVSILDTCY 408

Query: 446  NFTDYDEVNIPKISMLFKGGVTLDLDPSGILIGASASQVCLAFAGNQDDKDVGILGNKQQ 267
            + + Y  V +PKI + F GG  +DL P GI+     SQVCLAFAGN DD +V I+GN QQ
Sbjct: 409  DLSKYKTVKVPKIILYFSGGAEMDLAPEGIIYVLKVSQVCLAFAGNSDDDEVAIIGNVQQ 468

Query: 266  QKLEVIYDVARSRLGFVPGIC 204
            + + V+YD A  R+GF P  C
Sbjct: 469  KTIHVVYDDAEGRVGFAPSGC 489


>ref|XP_003532146.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Glycine max]
          Length = 488

 Score =  499 bits (1286), Expect = e-139
 Identities = 269/496 (54%), Positives = 347/496 (69%), Gaps = 10/496 (2%)
 Frame = -2

Query: 1658 VYTSFIVFVSL---LCFG--EKSFAYG---RDEVSESIHQSTVHVIVVSTILPPKSCSSF 1503
            +  S  VFVSL    CF   EKSFA+     D  S ++HQ T H++ +S++LP  SCSS 
Sbjct: 3    IQMSSFVFVSLTILFCFSSLEKSFAFQTTKEDTESNNLHQYT-HLVHLSSLLPSSSCSSS 61

Query: 1502 TSFKDRRSIPLLKIVHRHSPCSGSFHPEGQKKRATTNQILSTEQILHQDQIRVESLHYKL 1323
                 R++   L++VH+H PCS   + +G+ K  T +      +IL+QD+ RV+ ++ ++
Sbjct: 62   AKGPKRKAS--LEVVHKHGPCSQLNNHDGKAKSKTPHS-----EILNQDKERVKYINSRI 114

Query: 1322 S-NKSKMSQEQSSKATVLPAKSGVSLGTGNYIVRVGFGTPKKYLSLMFDTGSDLTWIQCK 1146
            S N  + S      +  LPAKSG  +G+GNY V VG GTPK+ LSL+FDTGSDLTW QC+
Sbjct: 115  SKNLGQDSSVSELDSVTLPAKSGSLIGSGNYFVVVGLGTPKRDLSLIFDTGSDLTWTQCE 174

Query: 1145 PCVRYCHEQQESIFNPSESTSYSNITCNTSECSQLKSATGNTPACGSSS-TCIYGIQYGD 969
            PC R C++QQ++IF+PS+STSYSNITC ++ C+QL +ATGN P C +S+  CIYGIQYGD
Sbjct: 175  PCARSCYKQQDAIFDPSKSTSYSNITCTSTLCTQLSTATGNEPGCSASTKACIYGIQYGD 234

Query: 968  QSFSVGYFGRETLTLTPRDVFNNFRFGCGQNNRGIFGKTAGLLGLGRNELSFVSQTSQKY 789
             SFSVGYF RE L++T  D+ +NF FGCGQNN+G+FG +AGL+GLGR+ +SFV QT+  Y
Sbjct: 235  SSFSVGYFSRERLSVTATDIVDNFLFGCGQNNQGLFGGSAGLIGLGRHPISFVQQTAAVY 294

Query: 788  GGRFSYCLPSSSSSVGYLAFGVDRSVSSTVKFTPMVIDSRGPSFYFLEIIEINVGDRKLE 609
               FSYCLP++SSS G L+FG   + +S VK+TP    SRG SFY L+I  I+VG  KL 
Sbjct: 295  RKIFSYCLPATSSSTGRLSFGT--TTTSYVKYTPFSTISRGSSFYGLDITGISVGGAKLP 352

Query: 608  ISASVFSFSGMIIDSGTVITRLPKTAYTSLQSAFQQLMSSYPSAKPLSILDTCYNFTDYD 429
            +S+S FS  G IIDSGTVITRLP TAYT+L+SAF+Q MS YPSA  LSILDTCY+ + Y+
Sbjct: 353  VSSSTFSTGGAIIDSGTVITRLPPTAYTALRSAFRQGMSKYPSAGELSILDTCYDLSGYE 412

Query: 428  EVNIPKISMLFKGGVTLDLDPSGILIGASASQVCLAFAGNQDDKDVGILGNKQQQKLEVI 249
              +IPKI   F GGVT+ L P GIL  ASA QVCLAFA N DD DV I GN QQ+ +EV+
Sbjct: 413  VFSIPKIDFSFAGGVTVQLPPQGILYVASAKQVCLAFAANGDDSDVTIYGNVQQKTIEVV 472

Query: 248  YDVARSRLGFVPGICK 201
            YDV   R+GF  G CK
Sbjct: 473  YDVGGGRIGFGAGGCK 488


>ref|XP_002515388.1| Aspartic proteinase nepenthesin-2 precursor, putative [Ricinus
            communis] gi|223545332|gb|EEF46837.1| Aspartic proteinase
            nepenthesin-2 precursor, putative [Ricinus communis]
          Length = 494

 Score =  499 bits (1286), Expect = e-139
 Identities = 256/467 (54%), Positives = 329/467 (70%)
 Frame = -2

Query: 1604 FAYGRDEVSESIHQSTVHVIVVSTILPPKSCSSFTSFKDRRSIPLLKIVHRHSPCSGSFH 1425
            +A+   + +ES H  T   I ++++LP  SC   T      +   LK+VH+H PCS    
Sbjct: 42   YAFEGRKFAESQHTHTT--IHLTSLLPAASCKPSTQVPSIENKAFLKVVHKHGPCSDL-- 97

Query: 1424 PEGQKKRATTNQILSTEQILHQDQIRVESLHYKLSNKSKMSQEQSSKATVLPAKSGVSLG 1245
             +G K  A        + IL QDQ RV+S+H KLS  S +S  +++ AT LPAK G  +G
Sbjct: 98   RQGHKAEA--------QYILLQDQSRVDSIHSKLSKDSGLSDVKATAATTLPAKDGSIIG 149

Query: 1244 TGNYIVRVGFGTPKKYLSLMFDTGSDLTWIQCKPCVRYCHEQQESIFNPSESTSYSNITC 1065
            +GNY V VG GTPKK  SL+FDTGSDLTW QC+PCV+ C+ Q+E+IFNPS+STSY+NI+C
Sbjct: 150  SGNYFVTVGLGTPKKDFSLIFDTGSDLTWTQCEPCVKSCYNQKEAIFNPSQSTSYANISC 209

Query: 1064 NTSECSQLKSATGNTPACGSSSTCIYGIQYGDQSFSVGYFGRETLTLTPRDVFNNFRFGC 885
             ++ C  L SATGN   C +SSTC+YGIQYGD SFS+G+FG+E L+LT  DVFN+F FGC
Sbjct: 210  GSTLCDSLASATGNIFNC-ASSTCVYGIQYGDSSFSIGFFGKEKLSLTATDVFNDFYFGC 268

Query: 884  GQNNRGIFGKTAGLLGLGRNELSFVSQTSQKYGGRFSYCLPSSSSSVGYLAFGVDRSVSS 705
            GQNN+G+FG  AGLLGLGR++LS VSQT+Q+Y   FSYCLPSSSSS G+L FG   S S 
Sbjct: 269  GQNNKGLFGGAAGLLGLGRDKLSLVSQTAQRYNKIFSYCLPSSSSSTGFLTFG--GSTSK 326

Query: 704  TVKFTPMVIDSRGPSFYFLEIIEINVGDRKLEISASVFSFSGMIIDSGTVITRLPKTAYT 525
            +  FTP+   S G SFY L++  I+VG RKL IS SVFS +G IIDSGTVITRLP  AY+
Sbjct: 327  SASFTPLATISGGSSFYGLDLTGISVGGRKLAISPSVFSTAGTIIDSGTVITRLPPAAYS 386

Query: 524  SLQSAFQQLMSSYPSAKPLSILDTCYNFTDYDEVNIPKISMLFKGGVTLDLDPSGILIGA 345
            +L S F++LMS YP+A  LSILDTC++F+++D +++PKI + F GGV +D+D +GI    
Sbjct: 387  ALSSTFRKLMSQYPAAPALSILDTCFDFSNHDTISVPKIGLFFSGGVVVDIDKTGIFYVN 446

Query: 344  SASQVCLAFAGNQDDKDVGILGNKQQQKLEVIYDVARSRLGFVPGIC 204
              +QVCLAFAGN D  DV I GN QQ+ LEV+YD A  R+GF P  C
Sbjct: 447  DLTQVCLAFAGNSDASDVAIFGNVQQKTLEVVYDGAAGRVGFAPAGC 493