BLASTX nr result
ID: Coptis25_contig00003824
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00003824
(2015 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAJ40864.1| tetrahydroberberine oxidase [Coptis japonica] 1024 0.0
gb|ADY15026.1| (S)-tetrahydroprotoberberine oxidase [Berberis wi... 702 0.0
ref|XP_002332196.1| predicted protein [Populus trichocarpa] gi|2... 592 e-166
ref|XP_002277310.1| PREDICTED: reticuline oxidase-like protein-l... 570 e-160
ref|XP_002277294.1| PREDICTED: reticuline oxidase-like protein-l... 569 e-160
>dbj|BAJ40864.1| tetrahydroberberine oxidase [Coptis japonica]
Length = 540
Score = 1024 bits (2648), Expect = 0.0
Identities = 508/540 (94%), Positives = 513/540 (95%)
Frame = +1
Query: 85 MMGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIPVYNPSSTSYTSI 264
MMGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIP+YNPSSTSYTSI
Sbjct: 1 MMGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIPIYNPSSTSYTSI 60
Query: 265 LHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSSSVA 444
LHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGL IRTRSGGHDFEGSS VA
Sbjct: 61 LHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLLIRTRSGGHDFEGSSFVA 120
Query: 445 PVPFVLLDLIRLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGG 624
VPFVLLDLI LRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGG
Sbjct: 121 TVPFVLLDLIHLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGG 180
Query: 625 HISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILA 804
HISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILA
Sbjct: 181 HISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILA 240
Query: 805 WKXXXXXXXXXXXXXXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPE 984
WK EQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPE
Sbjct: 241 WKIRLVPVPPTVTTFVVPRVLEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPE 300
Query: 985 KKTIVISFLGFYLGAPEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLENDLSL 1164
KKTIVISFLGFYLGAPEK LQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLE DLSL
Sbjct: 301 KKTIVISFLGFYLGAPEKTLQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLETDLSL 360
Query: 1165 LLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRFDGIDEPEIIMSPYGGKMNEIEES 1344
LLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRF+ +DEPEIIMSPYGGKMNEIEES
Sbjct: 361 LLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRFNEVDEPEIIMSPYGGKMNEIEES 420
Query: 1345 AIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKSPRCSYLNFKDN 1524
AIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSK+PRCSYLNFKDN
Sbjct: 421 AIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKNPRCSYLNFKDN 480
Query: 1525 DLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQGIPPLQSLAEAKK 1704
DLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQ IPPL+SLAEAKK
Sbjct: 481 DLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQSIPPLRSLAEAKK 540
>gb|ADY15026.1| (S)-tetrahydroprotoberberine oxidase [Berberis wilsonae]
Length = 530
Score = 702 bits (1811), Expect = 0.0
Identities = 344/522 (65%), Positives = 417/522 (79%), Gaps = 4/522 (0%)
Frame = +1
Query: 133 AIANSLVP--LASSSSEYEGFLQCLDLRSNSSIPVYNPSSTSYTSILHASIYNLRFISPT 306
++ +SL+P LASS + YE FLQCLDL S +SIPVY +++SYTSIL ++I NL F+SPT
Sbjct: 13 SLLSSLLPTSLASSDANYEDFLQCLDLYSQNSIPVYTRNTSSYTSILESTIKNLVFLSPT 72
Query: 307 TPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSSSVAPVPFVLLDLIRLRT 486
TPKPNFI+TPM+ESHVQ +V+C R HGLQ+R RSGGHDFEG S V+ VPFV+LDLI L+T
Sbjct: 73 TPKPNFIVTPMQESHVQTSVICCRMHGLQMRIRSGGHDFEGLSYVSNVPFVVLDLIHLKT 132
Query: 487 ISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGGHISGAGYGILMRKY 666
I+V+IE+ +AWVQTGATIGELYYRIAEK HAFPAGLCP+VGVGGHISGAGYG+LMRKY
Sbjct: 133 INVDIEENSAWVQTGATIGELYYRIAEKVGVHAFPAGLCPTVGVGGHISGAGYGVLMRKY 192
Query: 667 GLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILAWKXXXXXXXXXXXX 846
G+SADHVIDAR+V+V+G ILD+ESMGED FWAIRGGGGASFGVILAWK
Sbjct: 193 GVSADHVIDARIVNVDGEILDRESMGEDLFWAIRGGGGASFGVILAWKIRLVPVPPTVTI 252
Query: 847 XXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPEKKTIVISFLGFYLG 1026
E+GATAL+HKWQ I D + +DLF+GLS++++ + P KTI++SF+G +LG
Sbjct: 253 FIVPKTLEEGATALLHKWQFIGDNVHEDLFIGLSMRSVIISP--KGDKTILVSFIGLFLG 310
Query: 1027 APEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLENDLSLLLERKNPIPPKNRY 1206
+K +Q ME+SFPELG+ DCIEMSWIKS + FG+F + LS+LL+RKNP PPK+ +
Sbjct: 311 GSDKLVQHMEQSFPELGVKPHDCIEMSWIKSTVVFGVFSNDASLSVLLDRKNPFPPKSYH 370
Query: 1207 MSKSDYVHEPVSEAVLEGIWKRF--DGIDEPEIIMSPYGGKMNEIEESAIAFPHRKGNMY 1380
KSDYV EP+ +VLEGI RF +G+++ EIIMSPYGG+MNEI ES IAFPHRKGN+Y
Sbjct: 371 KVKSDYVTEPLPISVLEGICHRFLKNGVNKAEIIMSPYGGRMNEISESEIAFPHRKGNLY 430
Query: 1381 KINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKSPRCSYLNFKDNDLGHKKNGTATY 1560
KINY+ W+E G + + H SWIRELY YMTPYVSKSPR SYLNFKD DLG KNGTATY
Sbjct: 431 KINYIAEWEEAG--SMENHLSWIRELYRYMTPYVSKSPRSSYLNFKDIDLGQTKNGTATY 488
Query: 1561 LEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQGIPPLQS 1686
+AK WGSKY+KNNF RL+ VKTKVDP++FF NEQGIPP S
Sbjct: 489 SQAKAWGSKYFKNNFKRLMQVKTKVDPNNFFCNEQGIPPFSS 530
>ref|XP_002332196.1| predicted protein [Populus trichocarpa] gi|222875303|gb|EEF12434.1|
predicted protein [Populus trichocarpa]
Length = 527
Score = 592 bits (1526), Expect = e-166
Identities = 295/528 (55%), Positives = 383/528 (72%), Gaps = 5/528 (0%)
Frame = +1
Query: 118 FLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIP--VYNPSSTSYTSILHASIYNLR 291
FL F+ + S V +S+ +E FLQCLD +++ SI +Y P ++SY+SIL SI NLR
Sbjct: 4 FLFFLIFSFSWV---TSAHTHESFLQCLDSQNSHSISKLIYTPINSSYSSILQFSIQNLR 60
Query: 292 FISPTTPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSSSVAPVPFVLLDL 471
F + +TPKP I+TP SH+QA ++CS++HG+QIR RSGGHD+EG S V+ FV++DL
Sbjct: 61 FNTSSTPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVIDL 120
Query: 472 IRLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGGHISGAGYGI 651
I LRTI+VN E++TAWVQ+G TIGE+YYRIAEKSRT AFPA +CP+VGVGGH SG GYG+
Sbjct: 121 INLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGYGM 180
Query: 652 LMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILAWKXXXXXXX 831
LMRKYGL+ADH+IDA+LVDV GRILD+ESMGED FWAIRGGGG +FGV++AWK
Sbjct: 181 LMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVSAP 240
Query: 832 XXXXXXXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPEKKTIVISFL 1011
EQ A+ LVH+WQ +ADKL +DLF+ + + ++ + TI SF
Sbjct: 241 PTVTVFNVQRTLEQNASKLVHRWQFVADKLHEDLFIRIILNRVNSTEEGKSNTTIQASFN 300
Query: 1012 GFYLGAPEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLENDLSLLLERKNPIP 1191
+LG ++ L L+++SFPELGL+KEDCIEMSWI+S LYF F + L +LL+R P
Sbjct: 301 SLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSLDVLLDR-TPST 359
Query: 1192 PKNRYMSKSDYVHEPVSEAVLEGIWKRF--DGIDEPEIIMSPYGGKMNEIEESAIAFPHR 1365
+N + +KSDYV EP+ E LEGIW+RF I+ P +I SPYGGKM+EI ES+I FPHR
Sbjct: 360 RRN-FKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISESSIPFPHR 418
Query: 1366 KGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKSPRCSYLNFKDNDLG-HKK 1542
GN+YKI +L+ W E G A K+H SWIR LY+Y+ PYVSK+PR +Y+N++D D+G +
Sbjct: 419 AGNIYKIQHLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRDLDIGINNH 478
Query: 1543 NGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQGIPPLQS 1686
G +Y +A +WG KY+KNNFDRLV VKT VDP +FF NEQ IPPL S
Sbjct: 479 AGNTSYRQASIWGLKYFKNNFDRLVRVKTAVDPANFFRNEQSIPPLSS 526
>ref|XP_002277310.1| PREDICTED: reticuline oxidase-like protein-like [Vitis vinifera]
Length = 537
Score = 570 bits (1468), Expect = e-160
Identities = 284/539 (52%), Positives = 374/539 (69%), Gaps = 6/539 (1%)
Frame = +1
Query: 88 MGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIPV----YNPSSTSY 255
MG SS LVF + +S V A+SSS ++ FLQCL L SNSS P+ Y P ++SY
Sbjct: 1 MGSSSSAETYILVFTLLFSS-VSWAASSSVHQNFLQCLTLNSNSSTPITKVLYTPKNSSY 59
Query: 256 TSILHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSS 435
++L SI NLRF S TPKP I+TP+ SH+QA V+CS+++GLQIR RSGGHD+EG S
Sbjct: 60 ETVLDFSIQNLRFTSSCTPKPQIIVTPLHVSHIQAAVICSKKYGLQIRARSGGHDYEGLS 119
Query: 436 SVAPVPFVLLDLIRLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVG 615
V+ VPF+++DL+ LR+I+V++ED +AWV+ GAT+GE+YY IA K+ H FPAG+CP+VG
Sbjct: 120 YVSEVPFIIVDLLELRSINVDVEDGSAWVEAGATLGEVYYSIANKTAIHGFPAGICPTVG 179
Query: 616 VGGHISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGV 795
VGGH+SG GYG L+RKYGL+AD++IDA +VD NG +L++ESMGED FWAIRGGGGASFG+
Sbjct: 180 VGGHLSGGGYGTLLRKYGLAADNIIDAYIVDSNGTLLNRESMGEDLFWAIRGGGGASFGI 239
Query: 796 ILAWKXXXXXXXXXXXXXXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPD 975
I++WK EQ A ++ KWQ +ADKL +DLF+ + VQA ++
Sbjct: 240 IVSWKIKLVPVPSTVTVFRVTRTLEQDAEKILLKWQQVADKLHEDLFIRVYVQA--VNGS 297
Query: 976 HPEKKTIVISFLGFYLGAPEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLEND 1155
++TI ++ +LG L LM ESFPELGL +DC E SWI+S LYF F
Sbjct: 298 QEGERTISSTYESLFLGNTSGLLSLMNESFPELGLAADDCNETSWIESVLYFAGFS-GQP 356
Query: 1156 LSLLLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRFDGIDEPE--IIMSPYGGKMN 1329
L +LL R KN + +KSD++ EP+ E L+GIWK F + +I+SPYGG+MN
Sbjct: 357 LDVLLNRSQ--TSKNYFKNKSDFLKEPIPETGLQGIWKLFYQVKNATALMIISPYGGRMN 414
Query: 1330 EIEESAIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKSPRCSYL 1509
EI E+ FPHRKG++Y I Y+V+W E G++ K+H W R+L+ YM PYVSKSPR +YL
Sbjct: 415 EIPETETPFPHRKGSLYSIQYVVAWLEEGKKVSKRHIDWARKLHKYMAPYVSKSPRAAYL 474
Query: 1510 NFKDNDLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQGIPPLQS 1686
N++D DLG KNG +Y +A +WG KYYK NF+RLV VKTKVDP +FF NEQ IPPL S
Sbjct: 475 NYRDLDLGRNKNGNTSYAQASIWGLKYYKINFNRLVQVKTKVDPSNFFRNEQSIPPLSS 533
>ref|XP_002277294.1| PREDICTED: reticuline oxidase-like protein-like [Vitis vinifera]
Length = 536
Score = 569 bits (1467), Expect = e-160
Identities = 286/535 (53%), Positives = 371/535 (69%), Gaps = 6/535 (1%)
Frame = +1
Query: 88 MGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIP----VYNPSSTSY 255
MG SS LVF + +S V A+SSS ++ FLQCL L SNSS P VY P ++SY
Sbjct: 1 MGSSSCAETYILVFTLLFSS-VSWAASSSVHQNFLQCLTLNSNSSTPITKVVYTPHNSSY 59
Query: 256 TSILHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSS 435
++L SI NLRF S TP+P I+TP+ SH+QA V+CS+++GLQIR RSGGHD+EG S
Sbjct: 60 ETVLDFSIQNLRFTSSCTPRPQVIVTPLLVSHIQAAVICSKKYGLQIRARSGGHDYEGLS 119
Query: 436 SVAPVPFVLLDLIRLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVG 615
V+ VPF+++DL+ LR+I+V++ED +AWV+ GAT+GE+YY IA K+ TH FPAG+CP+VG
Sbjct: 120 YVSEVPFIIVDLLELRSINVDVEDGSAWVEAGATLGEVYYSIANKTATHGFPAGICPTVG 179
Query: 616 VGGHISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGV 795
VGGH+SG GYG L+RKYGL+AD++IDA +VD NG +L++ESMGED FWAIRGGGGASFG+
Sbjct: 180 VGGHLSGGGYGTLLRKYGLAADNIIDAYIVDSNGTLLNRESMGEDLFWAIRGGGGASFGI 239
Query: 796 ILAWKXXXXXXXXXXXXXXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPD 975
I++WK EQ A ++ KWQ +ADKL +DLF+ + VQA ++
Sbjct: 240 IVSWKIKLVPVPSTVTVFTVTRTLEQDAEKILLKWQQVADKLHEDLFIRVYVQA--VNGS 297
Query: 976 HPEKKTIVISFLGFYLGAPEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLEND 1155
++TI ++ +LG L LM ESFPELGL +DC E SWI+S LYF F
Sbjct: 298 QEGERTISSTYESLFLGNTSGLLSLMNESFPELGLAADDCNETSWIESVLYFAGFS-GQP 356
Query: 1156 LSLLLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRFDGIDEPE--IIMSPYGGKMN 1329
L +LL R KN + +KSD++ EP+ E L GIWK F + II+SPYGG+MN
Sbjct: 357 LDVLLNRSQ--TSKNYFKNKSDFLKEPIPETGLHGIWKLFYELKNATGMIIISPYGGRMN 414
Query: 1330 EIEESAIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKSPRCSYL 1509
EI E+ FPHRKG++Y I Y+V+W E GEE K+H W R+LY YM PYVSKSPR +YL
Sbjct: 415 EIPETETPFPHRKGSLYSIQYVVNWLEEGEEVSKRHIDWTRKLYKYMAPYVSKSPRAAYL 474
Query: 1510 NFKDNDLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQGIP 1674
N++D DLG KNG +Y +A +WG KYYK NF+RLV VKTKVDP +FF NEQ IP
Sbjct: 475 NYRDLDLGRNKNGNTSYAQASIWGLKYYKINFNRLVQVKTKVDPSNFFRNEQSIP 529