BLASTX nr result
ID: Coptis25_contig00003814
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00003814
(1925 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002275003.1| PREDICTED: RAN GTPase-activating protein 1 i... 450 e-124
emb|CAN73875.1| hypothetical protein VITISV_039541 [Vitis vinifera] 450 e-124
ref|XP_002314715.1| predicted protein [Populus trichocarpa] gi|2... 447 e-123
ref|XP_002517205.1| leucine rich repeat-containing protein, puta... 447 e-123
gb|AAF19528.1|AF215731_1 Ran GTPase activating protein [Medicago... 445 e-122
>ref|XP_002275003.1| PREDICTED: RAN GTPase-activating protein 1 isoform 2 [Vitis vinifera]
gi|225436922|ref|XP_002274973.1| PREDICTED: RAN
GTPase-activating protein 1 isoform 1 [Vitis vinifera]
Length = 541
Score = 450 bits (1158), Expect = e-124
Identities = 238/330 (72%), Positives = 272/330 (82%), Gaps = 1/330 (0%)
Frame = +2
Query: 524 EPILLSLKDQLKEVDLSDIVAGKPEEEALEVMRIFSSALEDCVLRYLNLSENALGEKGVR 703
EPIL SLKDQL EVDLSD +AG+PE EAL+VM IFSSALE CVL+YLNLS NALGEKGVR
Sbjct: 174 EPILSSLKDQLMEVDLSDFIAGRPEAEALDVMNIFSSALEGCVLKYLNLSNNALGEKGVR 233
Query: 704 AFGDLLKSQGSLEELHLENNGISEEAAQAVYELIPSTEKLKVLHFHNNMTGDEGAIPISE 883
AFG LLKSQ +LEEL+L N+GISEEAA+AV ELIPSTEKL++L FHNNMTGDEGAI ISE
Sbjct: 234 AFGALLKSQNNLEELYLMNDGISEEAARAVCELIPSTEKLRILQFHNNMTGDEGAIAISE 293
Query: 884 VVKRSPALEDFRCSSTRVGSKGGLTLIEALRTCTNLKKLDLKDNMFDVGAGVALSKALSS 1063
+VKRSPALEDFRCSSTRV S+GG+ L +AL TCT LKKLDL+DNMF V AGVALSKALS+
Sbjct: 294 MVKRSPALEDFRCSSTRVDSEGGVALAKALGTCTRLKKLDLRDNMFGVEAGVALSKALSA 353
Query: 1064 HVGLVEIYLSYLNFEDNGAIAIANALQEFAPSLKVLDMAGNEITXXXXXXXXXXXXSKQS 1243
L E+YLSYLN ED GA AIANAL+E PSL+VL+MAGN+IT +KQ
Sbjct: 354 FKDLTEVYLSYLNLEDEGAKAIANALKESTPSLEVLEMAGNDITVEAASTLAACIAAKQC 413
Query: 1244 LTKLNLSDNELKDKGAILICKAL-EGHNQLKEVDMSANKIRRAGARVMGQTVIHKPEFKL 1420
L KL+L++NELKD+GAILI KAL EGHNQL EVD++ N IRRAGAR + QTV KP FKL
Sbjct: 414 LAKLSLAENELKDEGAILISKALEEGHNQLTEVDLNTNSIRRAGARFLAQTVASKPGFKL 473
Query: 1421 LEINGNFISDEGIDELNEMFKKNPEMLGRL 1510
L INGNFISDEGIDE+ ++FK P++LG L
Sbjct: 474 LNINGNFISDEGIDEVKDIFKNCPDVLGPL 503
Score = 172 bits (436), Expect = 3e-40
Identities = 88/124 (70%), Positives = 104/124 (83%), Gaps = 1/124 (0%)
Frame = +1
Query: 4 ISIKLWPPSQSTRVVLVERIVKNLSAPSIFSKRYGLLNKEEAEENAKHIEDTAFAAANEH 183
+SIKLWPPSQSTR++LVER+ KNL+ PSI S++YGLL +EEAEE AK IED AFA AN+H
Sbjct: 13 MSIKLWPPSQSTRLMLVERMTKNLTTPSILSRKYGLLMREEAEEYAKEIEDAAFATANQH 72
Query: 184 YEREPDGDGSSAVQLYAKESSKLMMEVLKRGPQT-VDREVSVDKDIPSTLKTVFDISKGK 360
YE+EPDGDGSSAVQLYAKESSKLM+E+LKRGP+T D EV + S L+TVFDIS G+
Sbjct: 73 YEKEPDGDGSSAVQLYAKESSKLMLEILKRGPKTKEDGEVMTAEQTTSPLETVFDISGGR 132
Query: 361 RANI 372
RA I
Sbjct: 133 RAFI 136
>emb|CAN73875.1| hypothetical protein VITISV_039541 [Vitis vinifera]
Length = 541
Score = 450 bits (1158), Expect = e-124
Identities = 238/330 (72%), Positives = 272/330 (82%), Gaps = 1/330 (0%)
Frame = +2
Query: 524 EPILLSLKDQLKEVDLSDIVAGKPEEEALEVMRIFSSALEDCVLRYLNLSENALGEKGVR 703
EPIL SLKDQL EVDLSD +AG+PE EAL+VM IFSSALE CVL+YLNLS NALGEKGVR
Sbjct: 174 EPILSSLKDQLMEVDLSDFIAGRPEAEALDVMNIFSSALEGCVLKYLNLSNNALGEKGVR 233
Query: 704 AFGDLLKSQGSLEELHLENNGISEEAAQAVYELIPSTEKLKVLHFHNNMTGDEGAIPISE 883
AFG LLKSQ +LEEL+L N+GISEEAA+AV ELIPSTEKL++L FHNNMTGDEGAI ISE
Sbjct: 234 AFGALLKSQNNLEELYLMNDGISEEAARAVCELIPSTEKLRILQFHNNMTGDEGAIAISE 293
Query: 884 VVKRSPALEDFRCSSTRVGSKGGLTLIEALRTCTNLKKLDLKDNMFDVGAGVALSKALSS 1063
+VKRSPALEDFRCSSTRV S+GG+ L +AL TCT LKKLDL+DNMF V AGVALSKALS+
Sbjct: 294 MVKRSPALEDFRCSSTRVDSEGGVALAKALGTCTRLKKLDLRDNMFGVEAGVALSKALSA 353
Query: 1064 HVGLVEIYLSYLNFEDNGAIAIANALQEFAPSLKVLDMAGNEITXXXXXXXXXXXXSKQS 1243
L E+YLSYLN ED GA AIANAL+E PSL+VL+MAGN+IT +KQ
Sbjct: 354 FKDLTEVYLSYLNLEDEGAKAIANALKESTPSLEVLEMAGNDITVEAASTLAACIAAKQC 413
Query: 1244 LTKLNLSDNELKDKGAILICKAL-EGHNQLKEVDMSANKIRRAGARVMGQTVIHKPEFKL 1420
L KL+L++NELKD+GAILI KAL EGHNQL EVD++ N IRRAGAR + QTV KP FKL
Sbjct: 414 LAKLSLAENELKDEGAILISKALEEGHNQLTEVDLNTNSIRRAGARFLAQTVASKPGFKL 473
Query: 1421 LEINGNFISDEGIDELNEMFKKNPEMLGRL 1510
L INGNFISDEGIDE+ ++FK P++LG L
Sbjct: 474 LNINGNFISDEGIDEVKDIFKNCPDVLGPL 503
Score = 169 bits (428), Expect = 2e-39
Identities = 87/124 (70%), Positives = 103/124 (83%), Gaps = 1/124 (0%)
Frame = +1
Query: 4 ISIKLWPPSQSTRVVLVERIVKNLSAPSIFSKRYGLLNKEEAEENAKHIEDTAFAAANEH 183
+SIKLW PSQSTR++LVER+ KNL+ PSI S++YGLL +EEAEE AK IED AFA AN+H
Sbjct: 13 MSIKLWAPSQSTRLMLVERMTKNLTTPSILSRKYGLLMREEAEEYAKEIEDAAFATANQH 72
Query: 184 YEREPDGDGSSAVQLYAKESSKLMMEVLKRGPQT-VDREVSVDKDIPSTLKTVFDISKGK 360
YE+EPDGDGSSAVQLYAKESSKLM+E+LKRGP+T D EV + S L+TVFDIS G+
Sbjct: 73 YEKEPDGDGSSAVQLYAKESSKLMLEILKRGPKTKEDGEVMTAEQTTSPLETVFDISGGR 132
Query: 361 RANI 372
RA I
Sbjct: 133 RAFI 136
>ref|XP_002314715.1| predicted protein [Populus trichocarpa] gi|222863755|gb|EEF00886.1|
predicted protein [Populus trichocarpa]
Length = 540
Score = 447 bits (1150), Expect = e-123
Identities = 235/328 (71%), Positives = 270/328 (82%), Gaps = 1/328 (0%)
Frame = +2
Query: 524 EPILLSLKDQLKEVDLSDIVAGKPEEEALEVMRIFSSALEDCVLRYLNLSENALGEKGVR 703
EPIL S+K+QLKEVDLSD +AG+PE EALEVM IFS+ALE +L+ LNLS NALGEKGVR
Sbjct: 174 EPILASIKNQLKEVDLSDFIAGRPEAEALEVMNIFSTALEGSILKSLNLSNNALGEKGVR 233
Query: 704 AFGDLLKSQGSLEELHLENNGISEEAAQAVYELIPSTEKLKVLHFHNNMTGDEGAIPISE 883
AFG LL+SQ LEEL+L NNGISEEAA+AV ELIP TEKL+VL FHNNMTGD+GA+ IS+
Sbjct: 234 AFGALLRSQSCLEELYLMNNGISEEAAEAVCELIPLTEKLRVLQFHNNMTGDKGALAISK 293
Query: 884 VVKRSPALEDFRCSSTRVGSKGGLTLIEALRTCTNLKKLDLKDNMFDVGAGVALSKALSS 1063
VVKRSP LEDFRCSSTRVGS+GG+ L EAL TCT+LKKLDL+DNMF V AGVALSKALS
Sbjct: 294 VVKRSPLLEDFRCSSTRVGSEGGVALSEALDTCTHLKKLDLRDNMFGVEAGVALSKALSK 353
Query: 1064 HVGLVEIYLSYLNFEDNGAIAIANALQEFAPSLKVLDMAGNEITXXXXXXXXXXXXSKQS 1243
+ GL E+YLSYLN ED GA+AIA AL+E APSL+VLD+AGN+IT KQ
Sbjct: 354 YAGLTEVYLSYLNLEDEGAMAIARALKESAPSLEVLDIAGNDITAEAAPIVAACIAEKQH 413
Query: 1244 LTKLNLSDNELKDKGAILICKAL-EGHNQLKEVDMSANKIRRAGARVMGQTVIHKPEFKL 1420
LTKLNL++NELKD+GAI I K L EGH QLKEVDMS N IRRAGARV+ + V+ KPEFK
Sbjct: 414 LTKLNLAENELKDEGAIQISKVLEEGHLQLKEVDMSINSIRRAGARVLARVVVQKPEFKF 473
Query: 1421 LEINGNFISDEGIDELNEMFKKNPEMLG 1504
L I+GNFISDEGIDE+ EMF+K P+ LG
Sbjct: 474 LNIDGNFISDEGIDEVKEMFEKFPDRLG 501
Score = 159 bits (403), Expect = 2e-36
Identities = 81/123 (65%), Positives = 100/123 (81%), Gaps = 1/123 (0%)
Frame = +1
Query: 7 SIKLWPPSQSTRVVLVERIVKNLSAPSIFSKRYGLLNKEEAEENAKHIEDTAFAAANEHY 186
SIKLWPPSQ+TR +LV R+ NL+ SIF+++YG L+KEEAEENAK IED AF+ ANEHY
Sbjct: 14 SIKLWPPSQNTRQMLVVRVTDNLTTKSIFTQKYGSLSKEEAEENAKKIEDGAFSTANEHY 73
Query: 187 EREPDGDGSSAVQLYAKESSKLMMEVLKRGPQTV-DREVSVDKDIPSTLKTVFDISKGKR 363
E+EPDGDGSSAVQLYAKE SK ++EVLKRGP + D+EV V +++ + TVFDISKG R
Sbjct: 74 EKEPDGDGSSAVQLYAKECSKQILEVLKRGPSSKDDKEVLVSEEVSAPRDTVFDISKGAR 133
Query: 364 ANI 372
+ I
Sbjct: 134 SFI 136
>ref|XP_002517205.1| leucine rich repeat-containing protein, putative [Ricinus communis]
gi|223543840|gb|EEF45368.1| leucine rich
repeat-containing protein, putative [Ricinus communis]
Length = 549
Score = 447 bits (1149), Expect = e-123
Identities = 241/368 (65%), Positives = 282/368 (76%), Gaps = 1/368 (0%)
Frame = +2
Query: 524 EPILLSLKDQLKEVDLSDIVAGKPEEEALEVMRIFSSALEDCVLRYLNLSENALGEKGVR 703
EPIL S+KDQLKEVDLSD +AG+PEEEAL+VM IFS+ALE +L+ L+LS NALGEKGVR
Sbjct: 183 EPILASIKDQLKEVDLSDFIAGRPEEEALDVMNIFSAALEGSILKSLDLSNNALGEKGVR 242
Query: 704 AFGDLLKSQGSLEELHLENNGISEEAAQAVYELIPSTEKLKVLHFHNNMTGDEGAIPISE 883
AFG LL+SQ LEEL+L N+GISEEAA+AV ELIPSTEKL++LHFHNNMTGD GA+ I+E
Sbjct: 243 AFGALLRSQSCLEELYLMNDGISEEAARAVCELIPSTEKLRILHFHNNMTGDPGALAIAE 302
Query: 884 VVKRSPALEDFRCSSTRVGSKGGLTLIEALRTCTNLKKLDLKDNMFDVGAGVALSKALSS 1063
V+KRS LEDFRCSSTR+G++GG+ L EAL TC++LKKLDL+DN+F V AGVALSK L
Sbjct: 303 VLKRSALLEDFRCSSTRIGAEGGIALSEALETCSHLKKLDLRDNVFGVEAGVALSKVLPK 362
Query: 1064 HVGLVEIYLSYLNFEDNGAIAIANALQEFAPSLKVLDMAGNEITXXXXXXXXXXXXSKQS 1243
H GL E+YLSYLN ED GAIAIANAL+E AP+L+VLDMAGN+IT KQ+
Sbjct: 363 HAGLTEVYLSYLNLEDEGAIAIANALKESAPALEVLDMAGNDITAEAAPVVSACVELKQN 422
Query: 1244 LTKLNLSDNELKDKGAILICKAL-EGHNQLKEVDMSANKIRRAGARVMGQTVIHKPEFKL 1420
L KLNL++NELKD+GAI I KAL EGH QLKEVDMS N I R GARV+ Q ++ KPEFKL
Sbjct: 423 LIKLNLAENELKDEGAIQISKALEEGHVQLKEVDMSTNSIGRVGARVLAQVMVQKPEFKL 482
Query: 1421 LEINGNFISDEGIDELNEMFKKNPEMLGRLXXXXXXXXXXXXXXXXXXXXXXXXLGSKLQ 1600
L IN N ISDEGIDE+ E+FKK PEMLG L L SKL+
Sbjct: 483 LNINENCISDEGIDEVKEIFKKCPEMLGPL-DENNPEGPDDDEESGEGDDNQHDLESKLK 541
Query: 1601 NLEVSQED 1624
NLEV+ ED
Sbjct: 542 NLEVTGED 549
Score = 155 bits (391), Expect = 5e-35
Identities = 82/133 (61%), Positives = 96/133 (72%), Gaps = 11/133 (8%)
Frame = +1
Query: 7 SIKLWPPSQSTRVVLVERIVKNLSAPSIFSKRYGLLNKEEAEENAKHIEDTAFAAANEHY 186
SIKLWPPSQ+TR +LV RI NL++ SIF+++YG L+KEEAEENAK IED AF AANEHY
Sbjct: 13 SIKLWPPSQNTRQMLVTRITDNLTSKSIFTQKYGSLSKEEAEENAKRIEDVAFVAANEHY 72
Query: 187 EREPDGDGSSAVQLYAKESSKLMMEVLKRGP-----------QTVDREVSVDKDIPSTLK 333
E EPDGDG SAVQ YAKE SKL++EVLKRGP + VD E K++ +
Sbjct: 73 ENEPDGDGGSAVQFYAKECSKLILEVLKRGPARKEGGEILASKQVDGEALASKEVAVSRD 132
Query: 334 TVFDISKGKRANI 372
VFDISKG RA I
Sbjct: 133 IVFDISKGPRAFI 145
>gb|AAF19528.1|AF215731_1 Ran GTPase activating protein [Medicago sativa subsp. x varia]
Length = 533
Score = 445 bits (1144), Expect = e-122
Identities = 233/329 (70%), Positives = 267/329 (81%)
Frame = +2
Query: 524 EPILLSLKDQLKEVDLSDIVAGKPEEEALEVMRIFSSALEDCVLRYLNLSENALGEKGVR 703
EP+L+S+KDQLKEVDLSD +AG+PE EA+EVM IFSSALE VLRYLNLS NA+GEKGVR
Sbjct: 173 EPMLISIKDQLKEVDLSDFIAGRPEAEAIEVMNIFSSALERAVLRYLNLSNNAMGEKGVR 232
Query: 704 AFGDLLKSQGSLEELHLENNGISEEAAQAVYELIPSTEKLKVLHFHNNMTGDEGAIPISE 883
AF LLKSQ LEEL+L N+GISEEAA+AV ELIPSTEKLKVLHFHNNMTGDEGA I+E
Sbjct: 233 AFRALLKSQNDLEELYLMNDGISEEAAKAVAELIPSTEKLKVLHFHNNMTGDEGAFAIAE 292
Query: 884 VVKRSPALEDFRCSSTRVGSKGGLTLIEALRTCTNLKKLDLKDNMFDVGAGVALSKALSS 1063
VVKRSPALEDFRCSSTRVGS+GG+ L EAL CT+LKKLDL+DNMF V AGVALSK +
Sbjct: 293 VVKRSPALEDFRCSSTRVGSEGGVALAEALGACTHLKKLDLRDNMFGVEAGVALSKVIPV 352
Query: 1064 HVGLVEIYLSYLNFEDNGAIAIANALQEFAPSLKVLDMAGNEITXXXXXXXXXXXXSKQS 1243
L EIYLSYLN ED+GA A+ANAL+E APSL+ LDMAGN+IT SKQ
Sbjct: 353 FADLTEIYLSYLNLEDDGAEALANALKESAPSLETLDMAGNDITAKATVSVAECISSKQF 412
Query: 1244 LTKLNLSDNELKDKGAILICKALEGHNQLKEVDMSANKIRRAGARVMGQTVIHKPEFKLL 1423
LTKLNLS+NELKD+GA LI KALEG QL EVD+S N I +GA+++ + V+ KP FKLL
Sbjct: 413 LTKLNLSENELKDEGAGLISKALEGRGQLSEVDLSTNLITWSGAKLLAEAVVQKPGFKLL 472
Query: 1424 EINGNFISDEGIDELNEMFKKNPEMLGRL 1510
IN NFISDEGIDEL ++FK +P+MLG L
Sbjct: 473 NINANFISDEGIDELKDIFKNSPDMLGPL 501
Score = 164 bits (414), Expect = 1e-37
Identities = 80/125 (64%), Positives = 106/125 (84%), Gaps = 1/125 (0%)
Frame = +1
Query: 1 SISIKLWPPSQSTRVVLVERIVKNLSAPSIFSKRYGLLNKEEAEENAKHIEDTAFAAANE 180
++SIKLWPPSQSTR++LVER+V+NL+ PSIFS++YGLLNK+EAE++AK IED AF A++
Sbjct: 12 TLSIKLWPPSQSTRLMLVERMVRNLTTPSIFSRKYGLLNKDEAEKDAKEIEDAAFVTASQ 71
Query: 181 HYEREPDGDGSSAVQLYAKESSKLMMEVLKRGPQTVDREVS-VDKDIPSTLKTVFDISKG 357
H+E+EPDGDGSSAVQ+YAKESSKLM+EVLKRGP+ + + + + ++TVFDIS G
Sbjct: 72 HFEKEPDGDGSSAVQIYAKESSKLMLEVLKRGPRGKEENGELISEKGDAAVETVFDISGG 131
Query: 358 KRANI 372
+RA I
Sbjct: 132 RRAFI 136