BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00003522
         (1625 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004149671.1| PREDICTED: pyruvate dehydrogenase E1 compone...   625   e-176
ref|XP_002305328.1| predicted protein [Populus trichocarpa] gi|1...   623   e-176
ref|NP_174304.1| pyruvate dehydrogenase E1 beta [Arabidopsis tha...   619   e-175
gb|AAB86804.1| pyruvate dehydrogenase E1 beta subunit [Arabidops...   619   e-175
ref|XP_004149670.1| PREDICTED: pyruvate dehydrogenase E1 compone...   619   e-175

>ref|XP_004149671.1| PREDICTED: pyruvate dehydrogenase E1 component subunit beta-like
            isoform 2 [Cucumis sativus]
          Length = 410

 Score =  625 bits (1611), Expect = e-176
 Identities = 331/409 (80%), Positives = 351/409 (85%), Gaps = 6/409 (1%)
 Frame = -2

Query: 1447 AAAATVSLSSVNST-TKRSLLFSP-KSISFSESKKGKFFACRFN-NGKETAFGMSPKVVE 1277
            A  A  +LS  NS  +  SLL  P +S+SF E +KG+FF  R +  G   AF  +P+   
Sbjct: 7    ALGAPSALSPPNSLHSNTSLLLHPSRSLSFFE-RKGRFFIVRSDVTGANHAF--TPR--S 61

Query: 1276 SDSKLVTSAVAEKADAS---TKTKSGHELLLFEALQEGLEEEMDRDPRVCVMGEDVGHYG 1106
                L+T+AVA KAD S   T +K GHELLLFEAL+EGLEEEMDRDPRVCVMGEDVGHYG
Sbjct: 62   RSHHLITNAVATKADTSSPSTTSKPGHELLLFEALREGLEEEMDRDPRVCVMGEDVGHYG 121

Query: 1105 GSYKVTKGLAKKFGDLRVLDTPIAENSFTGMGIGAAMSGLRPVIEGMNMGFLLLAFNQIS 926
            GSYKVTKGLA K+GDLRVLDTPIAENSFTGMGIGAAM+GLRP++EGMNMGFLLLAFNQIS
Sbjct: 122  GSYKVTKGLATKYGDLRVLDTPIAENSFTGMGIGAAMTGLRPIVEGMNMGFLLLAFNQIS 181

Query: 925  NNCGMLHYTSGGQFTXXXXXXXXXXXXRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAK 746
            NNCGMLHYTSGGQF             RQLGAEHSQRLESYFQSIPGIQMVACSTPYNAK
Sbjct: 182  NNCGMLHYTSGGQFKIPIVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAK 241

Query: 745  GLMKAAIRSENPVILFEHVLLYNLKESIPDEEYVCSLEEAEMVRPGEHVTILTYSRMRYH 566
            GLMKAAIRSENPVILFEHVLLYNLKE IPDEEY+CSLEEAEMVRPGEHVTILTYSRMRYH
Sbjct: 242  GLMKAAIRSENPVILFEHVLLYNLKERIPDEEYICSLEEAEMVRPGEHVTILTYSRMRYH 301

Query: 565  VMQAVKTLVNNGYDPEVIDIRSLKPFDLHTIGNSIKKTHRVLIVEECMRTGGIGASLTAA 386
            VMQA KTLVN GYDPEVIDIRSLKPFDLHTIGNS+KKTHRVLIVEECMRTGGIGASLTAA
Sbjct: 302  VMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAA 361

Query: 385  ITENFYDYLDAPIRCLSSQDVPTPYAGTLEDVTVVQPAQIVTAVEQLCQ 239
            ITENF+DYLDAPI CLSSQDVPTPYAGTLED TVVQPAQIVTAVEQLCQ
Sbjct: 362  ITENFHDYLDAPIVCLSSQDVPTPYAGTLEDWTVVQPAQIVTAVEQLCQ 410


>ref|XP_002305328.1| predicted protein [Populus trichocarpa] gi|118482251|gb|ABK93053.1|
            unknown [Populus trichocarpa] gi|222848292|gb|EEE85839.1|
            predicted protein [Populus trichocarpa]
          Length = 418

 Score =  623 bits (1606), Expect = e-176
 Identities = 321/415 (77%), Positives = 346/415 (83%), Gaps = 4/415 (0%)
 Frame = -2

Query: 1471 MSAVSQTIAAAATVSLSSV-NSTTKRSLLFSPKSISFSESKKGKFFACRFNNGKETAFGM 1295
            M+ + Q +  AA  + +S+ NS   + LL      S +E K   F   R +       G 
Sbjct: 1    MATIFQALGGAAAAAAASLTNSFDSKKLLLPSSRRSLAERKASSFLVVRSDGSLNLNLGS 60

Query: 1294 SPKVVESDSKLVTSAVAEKAD---ASTKTKSGHELLLFEALQEGLEEEMDRDPRVCVMGE 1124
            S     +  KL+T+AVA KAD   AS+ +K GHELLLFEAL+EGLEEEMDRD  VCVMGE
Sbjct: 61   SNGRARTVDKLITNAVATKADTSAASSASKPGHELLLFEALREGLEEEMDRDLHVCVMGE 120

Query: 1123 DVGHYGGSYKVTKGLAKKFGDLRVLDTPIAENSFTGMGIGAAMSGLRPVIEGMNMGFLLL 944
            DVGHYGGSYKVTKGLA+K+GDLRVLDTPIAENSFTGMGIGAAM+GLRP+IEGMNMGFLLL
Sbjct: 121  DVGHYGGSYKVTKGLAEKYGDLRVLDTPIAENSFTGMGIGAAMTGLRPIIEGMNMGFLLL 180

Query: 943  AFNQISNNCGMLHYTSGGQFTXXXXXXXXXXXXRQLGAEHSQRLESYFQSIPGIQMVACS 764
            AFNQISNNCGMLHYTSGGQFT            RQLGAEHSQRLESYFQSIPGIQMVACS
Sbjct: 181  AFNQISNNCGMLHYTSGGQFTIPVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACS 240

Query: 763  TPYNAKGLMKAAIRSENPVILFEHVLLYNLKESIPDEEYVCSLEEAEMVRPGEHVTILTY 584
            TPYNAKGLMKAAIRSENPVILFEHVLLYNLKE IPDEEY+C+LEEAEMVRPGEHVTILTY
Sbjct: 241  TPYNAKGLMKAAIRSENPVILFEHVLLYNLKERIPDEEYICNLEEAEMVRPGEHVTILTY 300

Query: 583  SRMRYHVMQAVKTLVNNGYDPEVIDIRSLKPFDLHTIGNSIKKTHRVLIVEECMRTGGIG 404
            SRMRYHVMQA KTLVN GYDPEVIDIRSLKPFDLH IGNS+KKTHRV+IVEECMRTGGIG
Sbjct: 301  SRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHMIGNSVKKTHRVMIVEECMRTGGIG 360

Query: 403  ASLTAAITENFYDYLDAPIRCLSSQDVPTPYAGTLEDVTVVQPAQIVTAVEQLCQ 239
            ASLTAAITENF+DYLDAPI CLSSQDVPTPYAGTLE+ TVVQPAQIVTAVEQLCQ
Sbjct: 361  ASLTAAITENFHDYLDAPIVCLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQLCQ 415


>ref|NP_174304.1| pyruvate dehydrogenase E1 beta [Arabidopsis thaliana]
            gi|12321636|gb|AAG50862.1|AC074176_11 pyruvate
            dehydrogenase E1 beta subunit, putative [Arabidopsis
            thaliana] gi|13605515|gb|AAK32751.1|AF361583_1
            At1g30120/T2H7_8 [Arabidopsis thaliana]
            gi|20334776|gb|AAM16249.1| At1g30120/T2H7_8 [Arabidopsis
            thaliana] gi|62321138|dbj|BAD94262.1| hypothetical
            protein [Arabidopsis thaliana]
            gi|332193060|gb|AEE31181.1| pyruvate dehydrogenase E1
            beta [Arabidopsis thaliana]
          Length = 406

 Score =  619 bits (1597), Expect = e-175
 Identities = 317/403 (78%), Positives = 343/403 (85%)
 Frame = -2

Query: 1447 AAAATVSLSSVNSTTKRSLLFSPKSISFSESKKGKFFACRFNNGKETAFGMSPKVVESDS 1268
            A AAT +LS+ NS   + L  +P   + S   + + +    ++  + +FG   +V  S  
Sbjct: 8    AGAATTTLSTFNSVDSKKLFVAPSRTNLSV--RSQRYIVAGSDASKKSFGSGLRVRHSQ- 64

Query: 1267 KLVTSAVAEKADASTKTKSGHELLLFEALQEGLEEEMDRDPRVCVMGEDVGHYGGSYKVT 1088
            KL+ +AVA K +A T   +GHELLLFEALQEGLEEEMDRDP VCVMGEDVGHYGGSYKVT
Sbjct: 65   KLIPNAVATK-EADTSASTGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGHYGGSYKVT 123

Query: 1087 KGLAKKFGDLRVLDTPIAENSFTGMGIGAAMSGLRPVIEGMNMGFLLLAFNQISNNCGML 908
            KGLA KFGDLRVLDTPI EN+FTGMGIGAAM+GLRPVIEGMNMGFLLLAFNQISNNCGML
Sbjct: 124  KGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQISNNCGML 183

Query: 907  HYTSGGQFTXXXXXXXXXXXXRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAA 728
            HYTSGGQFT            RQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAA
Sbjct: 184  HYTSGGQFTIPVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAA 243

Query: 727  IRSENPVILFEHVLLYNLKESIPDEEYVCSLEEAEMVRPGEHVTILTYSRMRYHVMQAVK 548
            IRSENPVILFEHVLLYNLKE IPDE+YVC+LEEAEMVRPGEH+TILTYSRMRYHVMQA K
Sbjct: 244  IRSENPVILFEHVLLYNLKEKIPDEDYVCNLEEAEMVRPGEHITILTYSRMRYHVMQAAK 303

Query: 547  TLVNNGYDPEVIDIRSLKPFDLHTIGNSIKKTHRVLIVEECMRTGGIGASLTAAITENFY 368
            TLVN GYDPEVIDIRSLKPFDLHTIGNS+KKTHRVLIVEECMRTGGIGASLTAAI ENF+
Sbjct: 304  TLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFH 363

Query: 367  DYLDAPIRCLSSQDVPTPYAGTLEDVTVVQPAQIVTAVEQLCQ 239
            DYLDAP+ CLSSQDVPTPYAGTLE+ TVVQPAQIVTAVEQLCQ
Sbjct: 364  DYLDAPVMCLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQLCQ 406


>gb|AAB86804.1| pyruvate dehydrogenase E1 beta subunit [Arabidopsis thaliana]
            gi|21593321|gb|AAM65270.1| pyruvate dehydrogenase E1 beta
            subunit, putative [Arabidopsis thaliana]
          Length = 406

 Score =  619 bits (1596), Expect = e-175
 Identities = 316/403 (78%), Positives = 343/403 (85%)
 Frame = -2

Query: 1447 AAAATVSLSSVNSTTKRSLLFSPKSISFSESKKGKFFACRFNNGKETAFGMSPKVVESDS 1268
            A AAT +LS+ NS   + L  +P   + S   + + +    ++  + +FG   +V  S  
Sbjct: 8    AGAATTTLSTFNSVDSKKLFVAPSRTNLSV--RSQRYIVAGSDASKKSFGSGLRVRHSQ- 64

Query: 1267 KLVTSAVAEKADASTKTKSGHELLLFEALQEGLEEEMDRDPRVCVMGEDVGHYGGSYKVT 1088
            KL+ +AVA K +A T   +GHELLLFEALQEGLEEEMDRDP VCVMGEDVGHYGGSYKVT
Sbjct: 65   KLIPNAVATK-EADTSASTGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGHYGGSYKVT 123

Query: 1087 KGLAKKFGDLRVLDTPIAENSFTGMGIGAAMSGLRPVIEGMNMGFLLLAFNQISNNCGML 908
            KGLA KFGDLRVLDTPI EN+FTGMGIGAAM+GLRPVIEGMNMGFLLLAFNQISNNCGML
Sbjct: 124  KGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQISNNCGML 183

Query: 907  HYTSGGQFTXXXXXXXXXXXXRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAA 728
            HYTSGGQFT            RQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAA
Sbjct: 184  HYTSGGQFTIPVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAA 243

Query: 727  IRSENPVILFEHVLLYNLKESIPDEEYVCSLEEAEMVRPGEHVTILTYSRMRYHVMQAVK 548
            IRSENPVILFEHVLLYNLKE IPDE+Y+C+LEEAEMVRPGEH+TILTYSRMRYHVMQA K
Sbjct: 244  IRSENPVILFEHVLLYNLKEKIPDEDYICNLEEAEMVRPGEHITILTYSRMRYHVMQAAK 303

Query: 547  TLVNNGYDPEVIDIRSLKPFDLHTIGNSIKKTHRVLIVEECMRTGGIGASLTAAITENFY 368
            TLVN GYDPEVIDIRSLKPFDLHTIGNS+KKTHRVLIVEECMRTGGIGASLTAAI ENF+
Sbjct: 304  TLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFH 363

Query: 367  DYLDAPIRCLSSQDVPTPYAGTLEDVTVVQPAQIVTAVEQLCQ 239
            DYLDAP+ CLSSQDVPTPYAGTLE+ TVVQPAQIVTAVEQLCQ
Sbjct: 364  DYLDAPVMCLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQLCQ 406


>ref|XP_004149670.1| PREDICTED: pyruvate dehydrogenase E1 component subunit beta-like
            isoform 1 [Cucumis sativus]
          Length = 437

 Score =  619 bits (1595), Expect = e-175
 Identities = 318/380 (83%), Positives = 334/380 (87%), Gaps = 4/380 (1%)
 Frame = -2

Query: 1366 FSESKKGKFFACRFN-NGKETAFGMSPKVVESDSKLVTSAVAEKADAS---TKTKSGHEL 1199
            F   +KG+FF  R +  G   AF  +P+       L+T+AVA KAD S   T +K GHEL
Sbjct: 62   FDSERKGRFFIVRSDVTGANHAF--TPR--SRSHHLITNAVATKADTSSPSTTSKPGHEL 117

Query: 1198 LLFEALQEGLEEEMDRDPRVCVMGEDVGHYGGSYKVTKGLAKKFGDLRVLDTPIAENSFT 1019
            LLFEAL+EGLEEEMDRDPRVCVMGEDVGHYGGSYKVTKGLA K+GDLRVLDTPIAENSFT
Sbjct: 118  LLFEALREGLEEEMDRDPRVCVMGEDVGHYGGSYKVTKGLATKYGDLRVLDTPIAENSFT 177

Query: 1018 GMGIGAAMSGLRPVIEGMNMGFLLLAFNQISNNCGMLHYTSGGQFTXXXXXXXXXXXXRQ 839
            GMGIGAAM+GLRP++EGMNMGFLLLAFNQISNNCGMLHYTSGGQF             RQ
Sbjct: 178  GMGIGAAMTGLRPIVEGMNMGFLLLAFNQISNNCGMLHYTSGGQFKIPIVIRGPGGVGRQ 237

Query: 838  LGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAAIRSENPVILFEHVLLYNLKESIP 659
            LGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAAIRSENPVILFEHVLLYNLKE IP
Sbjct: 238  LGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAAIRSENPVILFEHVLLYNLKERIP 297

Query: 658  DEEYVCSLEEAEMVRPGEHVTILTYSRMRYHVMQAVKTLVNNGYDPEVIDIRSLKPFDLH 479
            DEEY+CSLEEAEMVRPGEHVTILTYSRMRYHVMQA KTLVN GYDPEVIDIRSLKPFDLH
Sbjct: 298  DEEYICSLEEAEMVRPGEHVTILTYSRMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLH 357

Query: 478  TIGNSIKKTHRVLIVEECMRTGGIGASLTAAITENFYDYLDAPIRCLSSQDVPTPYAGTL 299
            TIGNS+KKTHRVLIVEECMRTGGIGASLTAAITENF+DYLDAPI CLSSQDVPTPYAGTL
Sbjct: 358  TIGNSVKKTHRVLIVEECMRTGGIGASLTAAITENFHDYLDAPIVCLSSQDVPTPYAGTL 417

Query: 298  EDVTVVQPAQIVTAVEQLCQ 239
            ED TVVQPAQIVTAVEQLCQ
Sbjct: 418  EDWTVVQPAQIVTAVEQLCQ 437