BLASTX nr result
ID: Coptis25_contig00003354
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00003354
(1646 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFK49084.1| unknown [Medicago truncatula] 114 2e-38
gb|AFK43265.1| unknown [Medicago truncatula] 113 4e-38
gb|AFK45620.1| unknown [Lotus japonicus] 113 8e-38
gb|AFS51729.1| tau class glutathione S-transferase [Glycine soja] 111 1e-37
ref|NP_001235279.1| uncharacterized protein LOC100527381 [Glycin... 111 1e-37
>gb|AFK49084.1| unknown [Medicago truncatula]
Length = 220
Score = 114 bits (286), Expect(3) = 2e-38
Identities = 51/98 (52%), Positives = 72/98 (73%)
Frame = +2
Query: 1001 QYIEEDLSNKSPLLLKYNPIHRKIPVLLQNGINSVMESLVILHYIDETWTTNLILSEEPY 1180
+Y EE+L+NKS LLKYNP+++K+P+L+ NG N + ESL+IL YIDETW N +LSE+PY
Sbjct: 35 KYSEENLANKSDDLLKYNPVYKKVPILVHNG-NPIAESLIILEYIDETWKNNPLLSEDPY 93
Query: 1181 *RSKTVFWARFINEKCIPRLWMACWIGVKENEKGIEEA 1294
R+ FW + +++K +P +W ACW K EK +EEA
Sbjct: 94 ERALARFWFKTLDDKILPAIWNACWSDEKGREKAVEEA 131
Score = 67.4 bits (163), Expect(3) = 2e-38
Identities = 35/84 (41%), Positives = 53/84 (63%), Gaps = 7/84 (8%)
Frame = +3
Query: 1284 LKKQKFFGGETI-------NFIAFWLGIID*VVGIHLLDLENFPTIDKGIKEFLSLRVVI 1442
+K +KFFGGET+ NFI +W+ I+ + G+ LL LE FP + K +EF++ V+
Sbjct: 142 IKDKKFFGGETLGLVDIAANFIGYWVYILQEIAGLELLTLEKFPKVYKWSQEFINHPVIK 201
Query: 1443 ESLPPHEDILAFFRSRCESISASK 1514
E LPP +++ AFF++ SASK
Sbjct: 202 EGLPPRDELFAFFKA-----SASK 220
Score = 26.2 bits (56), Expect(3) = 2e-38
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = +1
Query: 952 EVKLFGAWLSPFSRR 996
EV+L G W SPFS R
Sbjct: 8 EVRLLGKWASPFSNR 22
>gb|AFK43265.1| unknown [Medicago truncatula]
Length = 220
Score = 113 bits (283), Expect(3) = 4e-38
Identities = 50/98 (51%), Positives = 72/98 (73%)
Frame = +2
Query: 1001 QYIEEDLSNKSPLLLKYNPIHRKIPVLLQNGINSVMESLVILHYIDETWTTNLILSEEPY 1180
+Y EE+L+NKS LLKYNP+++K+P+L+ NG N + ESL+IL YIDETW N +LSE+PY
Sbjct: 35 KYFEENLANKSDDLLKYNPVYKKVPILVHNG-NPIAESLIILEYIDETWKNNPLLSEDPY 93
Query: 1181 *RSKTVFWARFINEKCIPRLWMACWIGVKENEKGIEEA 1294
R+ FW++ +++K + +W ACW K EK +EEA
Sbjct: 94 ERALARFWSKTLDDKILSAIWNACWSDEKGREKAVEEA 131
Score = 67.4 bits (163), Expect(3) = 4e-38
Identities = 35/84 (41%), Positives = 53/84 (63%), Gaps = 7/84 (8%)
Frame = +3
Query: 1284 LKKQKFFGGETI-------NFIAFWLGIID*VVGIHLLDLENFPTIDKGIKEFLSLRVVI 1442
+K +KFFGGET+ NFI +W+ I+ + G+ LL LE FP + K +EF++ V+
Sbjct: 142 IKDKKFFGGETLGLVDIAANFIGYWVYILQEIAGLELLTLEKFPKVYKWSQEFINHPVIK 201
Query: 1443 ESLPPHEDILAFFRSRCESISASK 1514
E LPP +++ AFF++ SASK
Sbjct: 202 EGLPPRDELFAFFKA-----SASK 220
Score = 26.2 bits (56), Expect(3) = 4e-38
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = +1
Query: 952 EVKLFGAWLSPFSRR 996
EV+L G W SPFS R
Sbjct: 8 EVRLLGKWASPFSNR 22
>gb|AFK45620.1| unknown [Lotus japonicus]
Length = 219
Score = 113 bits (282), Expect(3) = 8e-38
Identities = 52/97 (53%), Positives = 70/97 (72%)
Frame = +2
Query: 1004 YIEEDLSNKSPLLLKYNPIHRKIPVLLQNGINSVMESLVILHYIDETWTTNLILSEEPY* 1183
Y EEDL+NKS LLKYNP+++K+PVL+ NG N + ESLVIL YIDETW N L ++PY
Sbjct: 35 YSEEDLANKSADLLKYNPVYKKVPVLVHNG-NPLAESLVILEYIDETWKNNPFLPQDPYE 93
Query: 1184 RSKTVFWARFINEKCIPRLWMACWIGVKENEKGIEEA 1294
R+ FW++ +++K +P +W ACW K EK +EEA
Sbjct: 94 RAVARFWSKTLDDKILPPIWNACWSDEKGREKAVEEA 130
Score = 66.2 bits (160), Expect(3) = 8e-38
Identities = 31/75 (41%), Positives = 48/75 (64%), Gaps = 7/75 (9%)
Frame = +3
Query: 1284 LKKQKFFGGETI-------NFIAFWLGIID*VVGIHLLDLENFPTIDKGIKEFLSLRVVI 1442
LK +FFGGETI NFI +W+ I+ + G+ LL +E FP + K +EF++ V+
Sbjct: 141 LKDNRFFGGETIGLVDIAANFIGYWVSILQEIAGLELLTVEKFPKLFKWSQEFVNHPVIK 200
Query: 1443 ESLPPHEDILAFFRS 1487
E LPP +++ AFF++
Sbjct: 201 EGLPPRDELFAFFKA 215
Score = 26.6 bits (57), Expect(3) = 8e-38
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 952 EVKLFGAWLSPFSRRATI 1005
EV+L G W SPFS R +
Sbjct: 7 EVRLLGKWASPFSNRVDL 24
>gb|AFS51729.1| tau class glutathione S-transferase [Glycine soja]
Length = 219
Score = 111 bits (277), Expect(3) = 1e-37
Identities = 49/98 (50%), Positives = 70/98 (71%)
Frame = +2
Query: 1001 QYIEEDLSNKSPLLLKYNPIHRKIPVLLQNGINSVMESLVILHYIDETWTTNLILSEEPY 1180
+Y EEDL+NKS LL+YNP+H+K+PVL+ NG N + ESL+I+ YIDETW N +L +PY
Sbjct: 34 KYSEEDLANKSADLLRYNPVHKKVPVLVHNG-NPLPESLIIVEYIDETWKNNPLLPRDPY 92
Query: 1181 *RSKTVFWARFINEKCIPRLWMACWIGVKENEKGIEEA 1294
R+ FW++ +++K +P +W ACW EK +EEA
Sbjct: 93 ERALARFWSKTLDDKILPAIWNACWSDENGREKAVEEA 130
Score = 69.3 bits (168), Expect(3) = 1e-37
Identities = 32/79 (40%), Positives = 52/79 (65%), Gaps = 7/79 (8%)
Frame = +3
Query: 1272 MRRALKKQKFFGGETI-------NFIAFWLGIID*VVGIHLLDLENFPTIDKGIKEFLSL 1430
++ ALK +KFFGGE+I NFI +W+ I+ + G+ LL +E FP + K +EF++
Sbjct: 137 LQEALKDKKFFGGESIGLVDIAANFIGYWVAILQEIAGLELLTIEKFPKLYKWSQEFINH 196
Query: 1431 RVVIESLPPHEDILAFFRS 1487
V+ E LPP +++ AFF++
Sbjct: 197 PVIKEGLPPRDELFAFFQA 215
Score = 24.6 bits (52), Expect(3) = 1e-37
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +1
Query: 952 EVKLFGAWLSPFSRRATI 1005
EV L G W SPFS R +
Sbjct: 7 EVILLGKWASPFSNRVDL 24
>ref|NP_001235279.1| uncharacterized protein LOC100527381 [Glycine max]
gi|255632224|gb|ACU16470.1| unknown [Glycine max]
Length = 219
Score = 111 bits (277), Expect(3) = 1e-37
Identities = 49/98 (50%), Positives = 70/98 (71%)
Frame = +2
Query: 1001 QYIEEDLSNKSPLLLKYNPIHRKIPVLLQNGINSVMESLVILHYIDETWTTNLILSEEPY 1180
+Y EEDL+NKS LL+YNP+H+K+PVL+ NG N + ESL+I+ YIDETW N +L +PY
Sbjct: 34 KYSEEDLANKSADLLRYNPVHKKVPVLVHNG-NPLPESLIIVEYIDETWKNNPLLPRDPY 92
Query: 1181 *RSKTVFWARFINEKCIPRLWMACWIGVKENEKGIEEA 1294
R+ FW++ +++K +P +W ACW EK +EEA
Sbjct: 93 ERALARFWSKTLDDKILPAIWNACWSDENGREKAVEEA 130
Score = 69.3 bits (168), Expect(3) = 1e-37
Identities = 32/79 (40%), Positives = 52/79 (65%), Gaps = 7/79 (8%)
Frame = +3
Query: 1272 MRRALKKQKFFGGETI-------NFIAFWLGIID*VVGIHLLDLENFPTIDKGIKEFLSL 1430
++ ALK +KFFGGE+I NFI +W+ I+ + G+ LL +E FP + K +EF++
Sbjct: 137 LQEALKDKKFFGGESIGLVDIAANFIGYWVAILQEIAGLELLTIEKFPKLYKWSQEFINH 196
Query: 1431 RVVIESLPPHEDILAFFRS 1487
V+ E LPP +++ AFF++
Sbjct: 197 PVIKEGLPPRDELFAFFQA 215
Score = 24.6 bits (52), Expect(3) = 1e-37
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +1
Query: 952 EVKLFGAWLSPFSRRATI 1005
EV L G W SPFS R +
Sbjct: 7 EVILLGKWASPFSNRVDL 24