BLASTX nr result
ID: Coptis25_contig00003003
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00003003
(2144 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif... 592 e-166
ref|XP_002275905.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif... 578 e-162
ref|XP_002279531.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif... 577 e-162
ref|XP_002334340.1| cytochrome P450 [Populus trichocarpa] gi|222... 561 e-157
ref|XP_002321857.1| cytochrome P450 [Populus trichocarpa] gi|222... 560 e-157
>ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
Length = 502
Score = 592 bits (1527), Expect = e-166
Identities = 291/505 (57%), Positives = 366/505 (72%), Gaps = 1/505 (0%)
Frame = +3
Query: 132 MEFMGYPETILAVVCFLFLHHLSNKS-LPTNWPFIGMLPALALNSQRVHDWSTYILSQTG 308
M +GYPE +LAV+CFL LH LSN + LP NWP +GMLP + N Q++ D T +L Q+
Sbjct: 1 MASIGYPEILLAVICFLLLHRLSNSNMLPWNWPLVGMLPWIFRNIQQIQDRCTEVLEQSS 60
Query: 309 CTFWFKGPWFANMNLLCTSDPANMRHIFSTNFSNYPKGTEFKEFFDILGDGIFNSDFESW 488
TF KGPWFANM++L TSDPAN+ +I STNFSN+PKG EF + FD+LG+GIFNSD + W
Sbjct: 61 GTFLLKGPWFANMDMLITSDPANVHYIMSTNFSNFPKGPEFLKIFDVLGEGIFNSDSDLW 120
Query: 489 KTQRKTAHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQNRVVDLQDLFKRFTFDNT 668
+ QRK A LIN+++FQ FL +T+ +KV GLIPVLEHV +Q +VDLQDLF+RFTFD T
Sbjct: 121 RKQRKLAQVLINDRQFQQFLVKTTENKVDEGLIPVLEHVSKQGLLVDLQDLFQRFTFDTT 180
Query: 669 IILLSGIDPGSLSIEFNTFPYAEALDDAEEAIFYRHIMPTSCWKFKRLLGIGTEKKLSKA 848
L++G D LSIEF +A+A+DD EEAIF+RH++P S WK +R LGIG EKKLSK
Sbjct: 181 CKLITGFDTKCLSIEFPRVLFAKAMDDVEEAIFFRHVVPESLWKLQRWLGIGEEKKLSKG 240
Query: 849 KESLDQFIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKYDKFLRDTILN 1028
E++D IA+ IS K E G DLLTSYM ED K +KFLRDTI+N
Sbjct: 241 WETIDNVIAEYISMKHEELSKGIAKLQEDEEGTDLLTSYMNEDSTMELKSNKFLRDTIVN 300
Query: 1029 FTVAGRDTVSAALTWFFWLLSKNPRVELQIIEELKRKAVSPMNQGKKWKSFDMGDLRGSV 1208
F +AGRDT SAALTWFFWL+SKNP VE +I EELK + + +KW F +L V
Sbjct: 301 FLLAGRDTTSAALTWFFWLVSKNPLVEAKIREELKE---TIPEKDQKWHIFRTEELNKLV 357
Query: 1209 YLHGALCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMEEVWGRDCL 1388
YLHGALCETLRL+P VPF+HK P + D+LPSGHRV K KIL LY+MGRM +WG+DC
Sbjct: 358 YLHGALCETLRLYPPVPFQHKAPHQADVLPSGHRVHPKMKILFSLYAMGRMTTIWGKDCF 417
Query: 1389 EFKPERWISDGGGIKYVPSYKFMAFNSGPRTCIGKEIAFTQMKIVVATLLYNYHVEVLEG 1568
EFKPERWI++ GGIKY PSYKF AFN+GPRTC+GK +AFTQMK V A +++NY+V++++G
Sbjct: 418 EFKPERWITEQGGIKYEPSYKFFAFNAGPRTCLGKGVAFTQMKAVAAAIIHNYNVQLVQG 477
Query: 1569 QSASPKNSIILYRNHGLTARIKRRF 1643
+P SIIL+ H L R+ +R+
Sbjct: 478 HPVAPNVSIILHMKHELMVRVTKRW 502
>ref|XP_002275905.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
Length = 507
Score = 578 bits (1490), Expect = e-162
Identities = 287/508 (56%), Positives = 367/508 (72%), Gaps = 4/508 (0%)
Frame = +3
Query: 132 MEFMGYPETILAVVCFLFLHHLSN-KSLPTNWPFIGMLPALALNSQRVHDWSTYILSQTG 308
M +GYPE +LAV+ F+ L N LP NWP +GM P++ L+ R+HD T IL ++G
Sbjct: 1 MALLGYPEILLAVIFFIILGRFWNINDLPWNWPLLGMFPSVFLHLHRIHDRVTDILEKSG 60
Query: 309 CTFWFKGPWFANMNLLCTSDPANMRHIFSTNFSNYPKGTEFKEFFDILGDGIFNSDFESW 488
CTF KGPWF NM+LL T+DPAN+ +I S++FSN+PKG+EF + FD LGDGIFNSD ESW
Sbjct: 61 CTFLVKGPWFCNMDLLVTTDPANVHYIMSSHFSNFPKGSEFSKIFDFLGDGIFNSDAESW 120
Query: 489 KTQRKTAHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQ-NRVVDLQDLFKRFTFDN 665
+ QRK +INN + FL + + +KV+ GL+P+L+++ EQ +R +DLQD+F+R TFD
Sbjct: 121 RKQRKLGQLMINNVRLYRFLGKITQNKVEKGLLPILDYLSEQEDRAMDLQDVFQRLTFDT 180
Query: 666 TIILLSGIDPGSLSIEFNTFPYAEALDDAEEAIFYRHIMPTSCWKFKRLLGIGTEKKLSK 845
T +L++G D LSIE P+A AL+DAEEAI +RH++P + W+ +R LGIG EKK K
Sbjct: 181 TCMLVTGFDSKCLSIELPEVPFATALEDAEEAILHRHVLPETLWRLQRWLGIGKEKKHQK 240
Query: 846 AKESLDQFIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKYD-KFLRDTI 1022
A E+LD IA+ IS KR+ G+DLLTSYM ED G K D KFLRDTI
Sbjct: 241 AWETLDHTIAEYISKKRDELSKGIINLQEEEDGVDLLTSYMSEDITMGLKCDHKFLRDTI 300
Query: 1023 LNFTVAGRDTVSAALTWFFWLLSKNPRVELQIIEELKRKAVSPMNQGKKWK-SFDMGDLR 1199
LNF +AGRDT S+ALTWFFWL+SKNP VE +I EEL+ + P + KK + +F L
Sbjct: 301 LNFMIAGRDTTSSALTWFFWLVSKNPLVESKIREELE--SAIPEKEDKKGRHTFKTEALN 358
Query: 1200 GSVYLHGALCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMEEVWGR 1379
VYLH ALCETLRL+P VPF+HK PL+PD+LPSGHRVD K KIL LY+MGRM +WG
Sbjct: 359 KLVYLHAALCETLRLYPPVPFQHKAPLQPDMLPSGHRVDPKMKILFSLYAMGRMTSIWGT 418
Query: 1380 DCLEFKPERWISDGGGIKYVPSYKFMAFNSGPRTCIGKEIAFTQMKIVVATLLYNYHVEV 1559
DCLEFKPERWIS+ G IK+ PSYKF AFN+GPRTC+GKE+AFTQMK V AT+++NYHV+V
Sbjct: 419 DCLEFKPERWISERGRIKHEPSYKFFAFNAGPRTCLGKEVAFTQMKAVAATIIHNYHVQV 478
Query: 1560 LEGQSASPKNSIILYRNHGLTARIKRRF 1643
+EG +P SIILY HGL R+ +R+
Sbjct: 479 VEGHHVAPNVSIILYMKHGLMVRVSKRW 506
>ref|XP_002279531.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
Length = 507
Score = 577 bits (1488), Expect = e-162
Identities = 279/506 (55%), Positives = 360/506 (71%), Gaps = 2/506 (0%)
Frame = +3
Query: 132 MEFMGYPETILAVVCFLFLHHL-SNKSLPTNWPFIGMLPALALNSQRVHDWSTYILSQTG 308
M + YPE ++AV+ FLF+ S K +P NWP +GM L + R+HD + IL +TG
Sbjct: 1 MALVSYPEILVAVIFFLFIGGFCSKKGVPWNWPIVGMFAGLLFHVHRIHDEAAEILEKTG 60
Query: 309 CTFWFKGPWFANMNLLCTSDPANMRHIFSTNFSNYPKGTEFKEFFDILGDGIFNSDFESW 488
CTF FKGPWF NM++L T+DPAN+ +I S+NFSN+PKGTEF++ F++LGDGIFN+D ESW
Sbjct: 61 CTFLFKGPWFCNMDMLGTTDPANVHYIMSSNFSNFPKGTEFRKMFEVLGDGIFNADGESW 120
Query: 489 KTQRKTAHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQNRVVDLQDLFKRFTFDNT 668
+ QRK +INN +F FL + + +KV+ GL+P+L+H+ EQ RVVDLQD+F+R TFD T
Sbjct: 121 RNQRKLGQSMINNPRFHRFLAKVTYNKVEKGLVPLLDHLSEQGRVVDLQDVFQRLTFDTT 180
Query: 669 IILLSGIDPGSLSIEFNTFPYAEALDDAEEAIFYRHIMPTSCWKFKRLLGIGTEKKLSKA 848
+L++G D LSIEF P+A AL+D EEA+FYRH +P + W+ R LGIG EKKL K
Sbjct: 181 CMLVTGFDTKCLSIEFPEVPFATALEDVEEAVFYRHALPETLWRLLRWLGIGKEKKLRKG 240
Query: 849 KESLDQFIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKYD-KFLRDTIL 1025
++LD IA+ IS KRE G DLLTSYM ED G K D KFLRDTIL
Sbjct: 241 WKTLDHTIAEYISMKREELSKGITKLREDEDGADLLTSYMTEDNAIGLKCDGKFLRDTIL 300
Query: 1026 NFTVAGRDTVSAALTWFFWLLSKNPRVELQIIEELKRKAVSPMNQGKKWKSFDMGDLRGS 1205
NF +AGRDT S+ALTWFFWL+SKNP VE +I EE++ A+ K F L
Sbjct: 301 NFMIAGRDTTSSALTWFFWLVSKNPLVESKIREEIE-TAIPEKEDRKGRHIFKTEGLNKL 359
Query: 1206 VYLHGALCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMEEVWGRDC 1385
VYLH A+CE LRL+P VPF+HK P++PD+LPSGH+VD K KI +Y+MGRM +WG+DC
Sbjct: 360 VYLHAAMCEALRLYPPVPFQHKAPIQPDVLPSGHKVDPKMKIFFPVYAMGRMTAIWGKDC 419
Query: 1386 LEFKPERWISDGGGIKYVPSYKFMAFNSGPRTCIGKEIAFTQMKIVVATLLYNYHVEVLE 1565
LEFKPERWIS+ G IK+ PSYKF +FN+GPRTCIGKE+AFTQMK V AT+++NYHVE+++
Sbjct: 420 LEFKPERWISELGKIKHEPSYKFFSFNAGPRTCIGKEVAFTQMKAVAATIIHNYHVEMVK 479
Query: 1566 GQSASPKNSIILYRNHGLTARIKRRF 1643
G +P SIILY G R+ +R+
Sbjct: 480 GHHVAPNVSIILYMKRGFKVRVSKRW 505
>ref|XP_002334340.1| cytochrome P450 [Populus trichocarpa] gi|222871210|gb|EEF08341.1|
cytochrome P450 [Populus trichocarpa]
Length = 506
Score = 561 bits (1447), Expect = e-157
Identities = 272/500 (54%), Positives = 359/500 (71%), Gaps = 3/500 (0%)
Frame = +3
Query: 153 ETILAVVCFLFLHHL--SNKSLPTNWPFIGMLPALALNSQRVHDWSTYILSQTGCTFWFK 326
E A +CFL L N LP NWP IGMLP L R HDW T +L Q+ CTF FK
Sbjct: 8 EIFFAFICFLSLIFCLRKNNGLPKNWPLIGMLPGLLFQVHRFHDWVTDVLEQSKCTFQFK 67
Query: 327 GPWFANMNLLCTSDPANMRHIFSTNFSNYPKGTEFKEFFDILGDGIFNSDFESWKTQRKT 506
GPWF +M++L T+DPAN+ +I S+NFSN+ KG++F + FDILGDGI N+D + WK+QRK
Sbjct: 68 GPWFGDMDILVTADPANIHYITSSNFSNFEKGSDFNKRFDILGDGIINADRDLWKSQRKV 127
Query: 507 AHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQNRVVDLQDLFKRFTFDNTIILLSG 686
A L+N++ F L +T KV+ GLIPVLEHV +Q+ V+DLQDLF+RFTFD T ++++G
Sbjct: 128 AQALVNHRLFYQRLVKTIQDKVERGLIPVLEHVYKQSLVLDLQDLFQRFTFDATCVIVTG 187
Query: 687 IDPGSLSIEFNTFPYAEALDDAEEAIFYRHIMPTSCWKFKRLLGIGTEKKLSKAKESLDQ 866
DPG LSI+ +++A+D+A EAIFYRH++P S W+ +R LGIG EKKL KA+E+LD+
Sbjct: 188 FDPGCLSIDLPEVAFSKAMDNAMEAIFYRHVLPESTWRLQRWLGIGKEKKLKKARETLDR 247
Query: 867 FIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKY-DKFLRDTILNFTVAG 1043
IA+ IS KRE GIDLLTSYM ED G K D+FLRDTI+ F +AG
Sbjct: 248 IIAEIISMKREELSKGNRLMEEDGEGIDLLTSYMSEDYNMGFKSDDEFLRDTIVTFMLAG 307
Query: 1044 RDTVSAALTWFFWLLSKNPRVELQIIEELKRKAVSPMNQGKKWKSFDMGDLRGSVYLHGA 1223
RDTVS+ L+WFFWL+SKNP +I EEL K P + +K + FD+ +++ VYLHGA
Sbjct: 308 RDTVSSCLSWFFWLVSKNPAAGAKIREEL--KTTLPEKEAEKRRLFDIEEIKKLVYLHGA 365
Query: 1224 LCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMEEVWGRDCLEFKPE 1403
LCE+LRL+P + E K P+R DILPSGHRV+ KTKI+ LY+MGRM VWG DC EFKPE
Sbjct: 366 LCESLRLYPPIHSEFKSPVRQDILPSGHRVNPKTKIVFSLYAMGRMSSVWGDDCFEFKPE 425
Query: 1404 RWISDGGGIKYVPSYKFMAFNSGPRTCIGKEIAFTQMKIVVATLLYNYHVEVLEGQSASP 1583
RWI+D GG+K+ PSYKF +FN+GPRTC+GK++AFTQMK VVA ++YNY V+V++ + +P
Sbjct: 426 RWITDRGGMKHEPSYKFFSFNAGPRTCLGKDVAFTQMKAVVAAIIYNYQVQVVDEHTVTP 485
Query: 1584 KNSIILYRNHGLTARIKRRF 1643
S+IL+ HG+ ++ +R+
Sbjct: 486 SQSVILHMKHGMKVKLTKRW 505
>ref|XP_002321857.1| cytochrome P450 [Populus trichocarpa] gi|222868853|gb|EEF05984.1|
cytochrome P450 [Populus trichocarpa]
Length = 506
Score = 560 bits (1442), Expect = e-157
Identities = 271/500 (54%), Positives = 358/500 (71%), Gaps = 3/500 (0%)
Frame = +3
Query: 153 ETILAVVCFLFLHHL--SNKSLPTNWPFIGMLPALALNSQRVHDWSTYILSQTGCTFWFK 326
E A +CFL L N LP NWP IGMLP L R HDW T +L Q+ CTF FK
Sbjct: 8 EIFFAFICFLSLIFCLRKNNGLPKNWPLIGMLPGLLFQVHRFHDWVTDVLEQSKCTFQFK 67
Query: 327 GPWFANMNLLCTSDPANMRHIFSTNFSNYPKGTEFKEFFDILGDGIFNSDFESWKTQRKT 506
GPWF +M++L T+DPAN+ +I S+NFSN+ KG++F + FDILGDGI N+D + WK+QRK
Sbjct: 68 GPWFGDMDILVTADPANIHYITSSNFSNFEKGSDFNKRFDILGDGIINADRDLWKSQRKV 127
Query: 507 AHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQNRVVDLQDLFKRFTFDNTIILLSG 686
A L+N++ F L +T KV+ GLIPVLEHV +Q+ V+DLQDLF+RFTFD T ++++G
Sbjct: 128 AQALVNHRLFYQRLVKTIQDKVERGLIPVLEHVYKQSLVLDLQDLFQRFTFDATCVIVTG 187
Query: 687 IDPGSLSIEFNTFPYAEALDDAEEAIFYRHIMPTSCWKFKRLLGIGTEKKLSKAKESLDQ 866
DPG LSI+ +++A+D+A EAIFYRH++P S W+ +R LGIG EKKL KA+E+LD+
Sbjct: 188 FDPGCLSIDLPEVAFSKAMDNAMEAIFYRHVLPESTWRLQRWLGIGKEKKLKKARETLDR 247
Query: 867 FIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKY-DKFLRDTILNFTVAG 1043
IA+ IS KRE GIDLLTSYM ED G K D+FLRDTI+ F +AG
Sbjct: 248 IIAEIISMKREELSKGNRLMEEDGEGIDLLTSYMSEDYNMGFKSDDEFLRDTIVTFMLAG 307
Query: 1044 RDTVSAALTWFFWLLSKNPRVELQIIEELKRKAVSPMNQGKKWKSFDMGDLRGSVYLHGA 1223
RDTVS+ L+WFFWL+SKNP +I EEL K P + +K + FD+ +++ VYLHGA
Sbjct: 308 RDTVSSCLSWFFWLVSKNPAAGAKIREEL--KTTLPEKEAEKRRLFDIEEIKKLVYLHGA 365
Query: 1224 LCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMEEVWGRDCLEFKPE 1403
LCE+LRL+P + E K P+R DILPSGHRV+ K KI+ LY+MGRM VWG DC EFKPE
Sbjct: 366 LCESLRLYPPIHSEFKSPVRQDILPSGHRVNPKAKIVFSLYAMGRMSSVWGDDCFEFKPE 425
Query: 1404 RWISDGGGIKYVPSYKFMAFNSGPRTCIGKEIAFTQMKIVVATLLYNYHVEVLEGQSASP 1583
RWI+D GG+K+ PSYKF +FN+GPRTC+GK++AFTQMK VVA ++YNY V+V++ + +P
Sbjct: 426 RWITDRGGMKHEPSYKFFSFNAGPRTCLGKDVAFTQMKAVVAAIIYNYQVQVVDEHTVTP 485
Query: 1584 KNSIILYRNHGLTARIKRRF 1643
S+IL+ HG+ ++ +R+
Sbjct: 486 SQSVILHMKHGMKVKLTKRW 505