BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00002933
         (1521 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       694   0.0  
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   535   e-164
ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]...   471   e-144
emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]                461   e-141
emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]                461   e-141

>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  694 bits (1790), Expect(2) = 0.0
 Identities = 343/357 (96%), Positives = 350/357 (98%)
 Frame = -1

Query: 1305 EVKSMVQRLSGQGCDYRTVEMKTMIFELTLNVMMTMIAGKRYFGGQVEELEQAKRFQEIV 1126
            EVKSMVQRLSGQG +YRTVEMKTMIFELTLNVMMTMIAGKRY+GGQVEELEQAKRFQEIV
Sbjct: 147  EVKSMVQRLSGQGNEYRTVEMKTMIFELTLNVMMTMIAGKRYYGGQVEELEQAKRFQEIV 206

Query: 1125 QGTLSISGASNIGDFLPILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHRISRNGGTQNK 946
            QGTL+ISGASNIGDFLPILSWIG+KGIEKKVWNLKEKRDKFLQELIEEHRISR+GG Q K
Sbjct: 207  QGTLAISGASNIGDFLPILSWIGIKGIEKKVWNLKEKRDKFLQELIEEHRISRSGGMQTK 266

Query: 945  EKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIK 766
            EKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIK
Sbjct: 267  EKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIK 326

Query: 765  KAQAEIDNTLEQGRLINESDVNKLPYLHSIINETLRIYPAGPLLVPHESSEECIVGGYNV 586
            KAQAEIDN LEQGRLINESDVNKLPYLHSII ETLRIYPAGPLLVPHESSEECIVGGY V
Sbjct: 327  KAQAEIDNNLEQGRLINESDVNKLPYLHSIITETLRIYPAGPLLVPHESSEECIVGGYKV 386

Query: 585  PSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGLAM 406
            PSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGLAM
Sbjct: 387  PSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGLAM 446

Query: 405  RVVGLALGALIQCFDWERVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTALNFISQF 235
            RVV LALGALIQCFDWERVGEEM+DMSEGPGLTLPKVHPL+AKCRPRSTALNFISQF
Sbjct: 447  RVVALALGALIQCFDWERVGEEMVDMSEGPGLTLPKVHPLEAKCRPRSTALNFISQF 503



 Score =  108 bits (270), Expect(2) = 0.0
 Identities = 51/53 (96%), Positives = 52/53 (98%)
 Frame = -3

Query: 1519 MGKILGHDYTTLLWAPYGPNWRNLRKISSIELLSANRLQSYSDIRHAEVKSMV 1361
            MGKILGHDYTTLLWAPYGPNWRNLRKIS+IELLSANRLQSYSDIRH EVKSMV
Sbjct: 100  MGKILGHDYTTLLWAPYGPNWRNLRKISAIELLSANRLQSYSDIRHDEVKSMV 152


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  535 bits (1377), Expect(2) = e-164
 Identities = 261/359 (72%), Positives = 305/359 (84%), Gaps = 3/359 (0%)
 Frame = -1

Query: 1305 EVKSMVQRLSGQGCDYRTVEMKTMIFELTLNVMMTMIAGKRYFGGQVEELEQAKRFQEIV 1126
            EVKSMV+RL   G +Y  VEMK++ FELTLN+MM MIAGKRY+G  V +LEQA++F+EIV
Sbjct: 149  EVKSMVRRLVA-GYEYHKVEMKSVFFELTLNIMMRMIAGKRYYGEHVADLEQARKFKEIV 207

Query: 1125 QGTLSISGASNIGDFLPILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHRISRNGGTQ-- 952
            + T  +SGASN+GDFLP+L WIG KGIEK++  LK KRD+FLQELI+E R  R       
Sbjct: 208  EETFELSGASNVGDFLPLLRWIGYKGIEKRLIRLKNKRDEFLQELIQERRKLRVNSKSFP 267

Query: 951  NKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQV 772
            ++EK  TLIDVLLSLQETEPEYYTDEMI G+IW+L AAGTDTSA TMEWAMSLLLNNP +
Sbjct: 268  SEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWAMSLLLNNPDL 327

Query: 771  IKKAQAEIDNTLEQGRLINESDVNKLPYLHSIINETLRIYPAGPLLVPHESSEECIVGGY 592
            IKKAQ+EID  +E GR I+ESD+NKLPYLH IINETLR+YPAGPLL+PHESSE+C +GGY
Sbjct: 328  IKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIPHESSEDCEIGGY 387

Query: 591  NVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFE-GTRDGFKLMPFGSGRRGCPGEG 415
            N+P GTMLLVN+WAIQ DPN+W EP KFKPERF+G++ G RDGFKLMPFGSGRRGCPGEG
Sbjct: 388  NIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGVRDGFKLMPFGSGRRGCPGEG 447

Query: 414  LAMRVVGLALGALIQCFDWERVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTALNFISQ 238
            LA RVVGL L +L+QCFDW RVGEEM++MSEG GLTLPK+HPL+A CRPRST LNF+SQ
Sbjct: 448  LAXRVVGLTLXSLLQCFDWHRVGEEMVEMSEGTGLTLPKLHPLEAHCRPRSTMLNFLSQ 506



 Score = 73.6 bits (179), Expect(2) = e-164
 Identities = 33/53 (62%), Positives = 43/53 (81%)
 Frame = -3

Query: 1519 MGKILGHDYTTLLWAPYGPNWRNLRKISSIELLSANRLQSYSDIRHAEVKSMV 1361
            +GK  G++YTTL WA YG NWRNLR+IS++E+ S NR+Q +S+IR  EVKSMV
Sbjct: 102  IGKHSGNNYTTLAWASYGQNWRNLRRISALEMFSPNRIQMFSEIRTDEVKSMV 154


>ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]
            gi|223550831|gb|EEF52317.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 500

 Score =  471 bits (1211), Expect(2) = e-144
 Identities = 228/357 (63%), Positives = 286/357 (80%), Gaps = 1/357 (0%)
 Frame = -1

Query: 1305 EVKSMVQRLSGQGCDYRT-VEMKTMIFELTLNVMMTMIAGKRYFGGQVEELEQAKRFQEI 1129
            EVK ++QRL     D R  V+MK+  FEL LNVMM MIAGKRY+G  VE++E+AKRF+EI
Sbjct: 146  EVKLLIQRLFRNNKDCREKVDMKSAFFELMLNVMMRMIAGKRYYGENVEQVEEAKRFREI 205

Query: 1128 VQGTLSISGASNIGDFLPILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHRISRNGGTQN 949
            V+ T   +G SN+GDFLP+++ +G  G EK++  L ++RD F+Q+LI+EHR  R     +
Sbjct: 206  VRETFLAAGTSNMGDFLPLVAVVG--GQEKRLKELGKRRDGFIQDLIDEHR-KRMAACSS 262

Query: 948  KEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVI 769
            +E+N T+I+VLLSLQE+EPEYYTDE I  ++ VLLAAGTDTSA TMEWAMSLL+NNP+ +
Sbjct: 263  EERNKTMIEVLLSLQESEPEYYTDETIKSLMLVLLAAGTDTSAATMEWAMSLLVNNPEAL 322

Query: 768  KKAQAEIDNTLEQGRLINESDVNKLPYLHSIINETLRIYPAGPLLVPHESSEECIVGGYN 589
            KKAQ EID+ +   RLINESD +KLPYL+ IINE +R+YPAGPLLVPHESSEEC +GGY 
Sbjct: 323  KKAQTEIDSVIGHDRLINESDTSKLPYLNCIINEVMRMYPAGPLLVPHESSEECFIGGYR 382

Query: 588  VPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGLA 409
            VP+GTMLLVN+W+IQ DP +W EP  FKPERF+G EG RDGF+LMPFGSGRR CPGEGLA
Sbjct: 383  VPAGTMLLVNLWSIQNDPRVWEEPRNFKPERFEGCEGVRDGFRLMPFGSGRRSCPGEGLA 442

Query: 408  MRVVGLALGALIQCFDWERVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTALNFISQ 238
            +R+VGL +G L+QCFDWERVG+EMIDM+EG GLT+PK  PL  +C PR + +N +SQ
Sbjct: 443  LRMVGLGIGTLLQCFDWERVGKEMIDMTEGVGLTMPKAQPLVVQCSPRPSMVNLLSQ 499



 Score = 69.3 bits (168), Expect(2) = e-144
 Identities = 30/52 (57%), Positives = 42/52 (80%)
 Frame = -3

Query: 1516 GKILGHDYTTLLWAPYGPNWRNLRKISSIELLSANRLQSYSDIRHAEVKSMV 1361
            GK +G++YT+L WAPYG  WRNLRK+S++E+LS++RLQ  S IR  EVK ++
Sbjct: 100  GKHIGYNYTSLAWAPYGDLWRNLRKVSALEILSSHRLQLLSSIRRDEVKLLI 151


>emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]
          Length = 520

 Score =  461 bits (1185), Expect(2) = e-141
 Identities = 222/358 (62%), Positives = 282/358 (78%), Gaps = 2/358 (0%)
 Frame = -1

Query: 1305 EVKSMVQRL-SGQGCDYRTVEMKTMIFELTLNVMMTMIAGKRYFGGQVEELEQ-AKRFQE 1132
            E + ++Q+L S        V MK   +ELTLNVMM MI+GKRYFGG   ELE+  KRF++
Sbjct: 164  ETRLLIQKLLSACNSGSSQVTMKFSFYELTLNVMMRMISGKRYFGGDNPELEEEGKRFRD 223

Query: 1131 IVQGTLSISGASNIGDFLPILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHRISRNGGTQ 952
            ++  T  ++GASN+GD+LP+LSW+GVKG+EKK+  L+EKRD F Q LI++ R S+  GT+
Sbjct: 224  MLDETFVLAGASNVGDYLPVLSWLGVKGLEKKLIKLQEKRDVFFQGLIDQLRKSK--GTE 281

Query: 951  NKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQV 772
            +  K  T+I++LLSLQETEPEYYTD MI   + VLLAAG+DTSA TMEW MSLLLN+PQV
Sbjct: 282  DVNKKKTMIELLLSLQETEPEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVMSLLLNHPQV 341

Query: 771  IKKAQAEIDNTLEQGRLINESDVNKLPYLHSIINETLRIYPAGPLLVPHESSEECIVGGY 592
            +KKAQ EID+ + +  L++ESD+  LPYL  IINETLR+YPAGPLLVPHE+S +C+VGGY
Sbjct: 342  LKKAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLYPAGPLLVPHEASSDCVVGGY 401

Query: 591  NVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGL 412
            NVP GT+L+VN WAI  DP +W EP  FKPERF+G EGTRDGFKL+PFGSGRR CPGEGL
Sbjct: 402  NVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTRDGFKLLPFGSGRRSCPGEGL 461

Query: 411  AMRVVGLALGALIQCFDWERVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTALNFISQ 238
            A+R++G+ LG++IQCFDWER  EE++DM+EGPGLT+PK  PL AKC+PR    N +S+
Sbjct: 462  AVRMLGMTLGSIIQCFDWERTSEELVDMTEGPGLTMPKAIPLVAKCKPRVEMTNLLSE 519



 Score = 68.9 bits (167), Expect(2) = e-141
 Identities = 29/52 (55%), Positives = 41/52 (78%)
 Frame = -3

Query: 1516 GKILGHDYTTLLWAPYGPNWRNLRKISSIELLSANRLQSYSDIRHAEVKSMV 1361
            GKI+G +YT+L W+PYG NWRNLR+I+SIE+LS +RL  + DIR  E + ++
Sbjct: 118  GKIIGVNYTSLAWSPYGDNWRNLRRIASIEILSIHRLNEFHDIRVEETRLLI 169


>emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]
          Length = 505

 Score =  461 bits (1185), Expect(2) = e-141
 Identities = 222/358 (62%), Positives = 282/358 (78%), Gaps = 2/358 (0%)
 Frame = -1

Query: 1305 EVKSMVQRL-SGQGCDYRTVEMKTMIFELTLNVMMTMIAGKRYFGGQVEELEQ-AKRFQE 1132
            E + ++Q+L S        V MK   +ELTLNVMM MI+GKRYFGG   ELE+  KRF++
Sbjct: 149  ETRLLIQKLLSACNSGSSQVTMKFSFYELTLNVMMRMISGKRYFGGDNPELEEEGKRFRD 208

Query: 1131 IVQGTLSISGASNIGDFLPILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHRISRNGGTQ 952
            ++  T  ++GASN+GD+LP+LSW+GVKG+EKK+  L+EKRD F Q LI++ R S+  GT+
Sbjct: 209  MLDETFVLAGASNVGDYLPVLSWLGVKGLEKKLIKLQEKRDVFFQGLIDQLRKSK--GTE 266

Query: 951  NKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQV 772
            +  K  T+I++LLSLQETEPEYYTD MI   + VLLAAG+DTSA TMEW MSLLLN+PQV
Sbjct: 267  DVNKKKTMIELLLSLQETEPEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVMSLLLNHPQV 326

Query: 771  IKKAQAEIDNTLEQGRLINESDVNKLPYLHSIINETLRIYPAGPLLVPHESSEECIVGGY 592
            +KKAQ EID+ + +  L++ESD+  LPYL  IINETLR+YPAGPLLVPHE+S +C+VGGY
Sbjct: 327  LKKAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLYPAGPLLVPHEASSDCVVGGY 386

Query: 591  NVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGL 412
            NVP GT+L+VN WAI  DP +W EP  FKPERF+G EGTRDGFKL+PFGSGRR CPGEGL
Sbjct: 387  NVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTRDGFKLLPFGSGRRSCPGEGL 446

Query: 411  AMRVVGLALGALIQCFDWERVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTALNFISQ 238
            A+R++G+ LG++IQCFDWER  EE++DM+EGPGLT+PK  PL AKC+PR    N +S+
Sbjct: 447  AVRMLGMTLGSIIQCFDWERTSEELVDMTEGPGLTMPKAIPLVAKCKPRVEMTNLLSE 504



 Score = 68.9 bits (167), Expect(2) = e-141
 Identities = 29/52 (55%), Positives = 41/52 (78%)
 Frame = -3

Query: 1516 GKILGHDYTTLLWAPYGPNWRNLRKISSIELLSANRLQSYSDIRHAEVKSMV 1361
            GKI+G +YT+L W+PYG NWRNLR+I+SIE+LS +RL  + DIR  E + ++
Sbjct: 103  GKIIGVNYTSLAWSPYGDNWRNLRRIASIEILSIHRLNEFHDIRVEETRLLI 154