BLASTX nr result
ID: Coptis25_contig00002872
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00002872
(1432 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003611801.1| Upstream activation factor subunit spp27 [Me... 295 3e-77
ref|XP_004148436.1| PREDICTED: upstream activation factor subuni... 291 2e-76
ref|XP_004170902.1| PREDICTED: uncharacterized protein LOC101226... 290 5e-76
emb|CAN83123.1| hypothetical protein VITISV_044372 [Vitis vinifera] 290 8e-76
ref|XP_003537360.1| PREDICTED: uncharacterized protein LOC100786... 286 9e-75
>ref|XP_003611801.1| Upstream activation factor subunit spp27 [Medicago truncatula]
gi|355513136|gb|AES94759.1| Upstream activation factor
subunit spp27 [Medicago truncatula]
Length = 361
Score = 295 bits (754), Expect = 3e-77
Identities = 151/234 (64%), Positives = 180/234 (76%), Gaps = 3/234 (1%)
Frame = +2
Query: 575 SNNKNKDVKRKGNGGGFNKVCSLSPELQQLLGVPELARTEVVKQLWAYIRENDLQDPSNR 754
SN +V +K GGGF K+CSLSP+LQ+ +G PE+ARTEVVKQLWAYIRE DLQDP+NR
Sbjct: 116 SNKSGDEVVKKKGGGGFCKICSLSPQLQEFVGAPEMARTEVVKQLWAYIREKDLQDPNNR 175
Query: 755 KNIKCDEPLRLLFRVDIINMFQMNKALSRHIWPLDSENVTSTANEKQRNKEKPEATVTSA 934
+NI CDEPLR LF VD INMFQMNK L++HIWPLDS++V K KEKP A
Sbjct: 176 RNIICDEPLRALFGVDTINMFQMNKVLAKHIWPLDSDDVIQV---KSAPKEKPAPKEKPA 232
Query: 935 PKEKRKQERHEDD--DESQVKEKRQR-RAGLGFLAPLPLSDALAKFIGTGESILPRSTVV 1105
KEK+K++ EDD DE + KEKRQ+ +G GFLAPL LSDALAKF+ GES L RS V+
Sbjct: 233 RKEKKKKQEREDDDLDEPKGKEKRQKVGSGKGFLAPLQLSDALAKFL--GESELSRSDVI 290
Query: 1106 KRIWDYIKANDLQDPNDKKMVLCDDKLKELFEVDSFTGFTVPKLLKTHFIKTER 1267
KR+WDYIK N+LQDP+DK+ +LCD+KLKELF+VDSF GFTV KLL HFIK E+
Sbjct: 291 KRMWDYIKGNNLQDPSDKRQILCDEKLKELFDVDSFVGFTVTKLLAPHFIKAEQ 344
Score = 85.1 bits (209), Expect = 4e-14
Identities = 41/57 (71%), Positives = 50/57 (87%)
Frame = +2
Query: 74 MVSDSELILRLREFLKTSDLNTTTNAIVRRKLEEDFGIDLSDKKAFIRSQVDIFIHS 244
MVS+SELI RLREFL+ SDLNTTT + VRR+LE DFGIDLSD+K+FIR QVD+F+ +
Sbjct: 1 MVSESELIDRLREFLRNSDLNTTTTSTVRRQLESDFGIDLSDRKSFIREQVDLFLQT 57
>ref|XP_004148436.1| PREDICTED: upstream activation factor subunit UAF30-like [Cucumis
sativus]
Length = 332
Score = 291 bits (746), Expect = 2e-76
Identities = 137/228 (60%), Positives = 177/228 (77%)
Frame = +2
Query: 584 KNKDVKRKGNGGGFNKVCSLSPELQQLLGVPELARTEVVKQLWAYIRENDLQDPSNRKNI 763
K K K GGGF K+CSLSP+LQ+ +G PE+ARTEVVKQLW +IREN+LQDPSNR+NI
Sbjct: 105 KTSSEKVKKRGGGFTKLCSLSPQLQEFIGAPEMARTEVVKQLWNHIRENNLQDPSNRRNI 164
Query: 764 KCDEPLRLLFRVDIINMFQMNKALSRHIWPLDSENVTSTANEKQRNKEKPEATVTSAPKE 943
CDEPL+ LF V+ INMFQMNKALS+HIWPL+S +V + ++ +K + K+
Sbjct: 165 LCDEPLKALFGVNSINMFQMNKALSKHIWPLESNDVIPAKSSQKEKPQKEKQPKEKPQKK 224
Query: 944 KRKQERHEDDDESQVKEKRQRRAGLGFLAPLPLSDALAKFIGTGESILPRSTVVKRIWDY 1123
++KQ + ED D+S ++KRQ++ GFLAPLPLS+AL F+GTGE LPRS VVKR+WDY
Sbjct: 225 RQKQGKEEDSDDSAREDKRQKKGKSGFLAPLPLSNALVAFLGTGEDALPRSDVVKRMWDY 284
Query: 1124 IKANDLQDPNDKKMVLCDDKLKELFEVDSFTGFTVPKLLKTHFIKTER 1267
IK N+LQDP+DK+ ++CD++LKELF+VDSF GFTV KLL THFIK ++
Sbjct: 285 IKQNNLQDPSDKRRIICDERLKELFDVDSFNGFTVSKLLATHFIKMKQ 332
Score = 94.7 bits (234), Expect = 5e-17
Identities = 47/60 (78%), Positives = 53/60 (88%)
Frame = +2
Query: 74 MVSDSELILRLREFLKTSDLNTTTNAIVRRKLEEDFGIDLSDKKAFIRSQVDIFIHSQVE 253
MVSDSELI RLR+FL+ SDLNTTT AIVRRKLEEDFGIDLSDKK FIR QVD+F+ ++ E
Sbjct: 1 MVSDSELISRLRDFLRNSDLNTTTTAIVRRKLEEDFGIDLSDKKLFIREQVDLFLQTEHE 60
Score = 77.0 bits (188), Expect = 1e-11
Identities = 52/197 (26%), Positives = 98/197 (49%), Gaps = 10/197 (5%)
Frame = +2
Query: 689 TEVVKQLWAYIRENDLQDPSN---RKNIK-------CDEPLRLLFRVDIINMFQMNKALS 838
+E++ +L ++R +DL + R+ ++ D+ L + +VD+ + KA+
Sbjct: 5 SELISRLRDFLRNSDLNTTTTAIVRRKLEEDFGIDLSDKKLFIREQVDLFLQTEHEKAVQ 64
Query: 839 RHIWPLDSENVTSTANEKQRNKEKPEATVTSAPKEKRKQERHEDDDESQVKEKRQRRAGL 1018
E Q + ++ T + +EDD++ + ++ ++ G
Sbjct: 65 --------EGYAHCEEVHQEDGDENLKMETEDGDSEDGDNDNEDDEKGKTSSEKVKKRGG 116
Query: 1019 GFLAPLPLSDALAKFIGTGESILPRSTVVKRIWDYIKANDLQDPNDKKMVLCDDKLKELF 1198
GF LS L +FIG E + R+ VVK++W++I+ N+LQDP++++ +LCD+ LK LF
Sbjct: 117 GFTKLCSLSPQLQEFIGAPE--MARTEVVKQLWNHIRENNLQDPSNRRNILCDEPLKALF 174
Query: 1199 EVDSFTGFTVPKLLKTH 1249
V+S F + K L H
Sbjct: 175 GVNSINMFQMNKALSKH 191
>ref|XP_004170902.1| PREDICTED: uncharacterized protein LOC101226406, partial [Cucumis
sativus]
Length = 225
Score = 290 bits (743), Expect = 5e-76
Identities = 136/223 (60%), Positives = 176/223 (78%)
Frame = +2
Query: 599 KRKGNGGGFNKVCSLSPELQQLLGVPELARTEVVKQLWAYIRENDLQDPSNRKNIKCDEP 778
K K GGGF K+CSLSP+LQ+ +G PE+ARTEVVKQLW +IREN+LQDPSNR+NI CDEP
Sbjct: 3 KVKKRGGGFTKLCSLSPQLQEFIGAPEMARTEVVKQLWNHIRENNLQDPSNRRNILCDEP 62
Query: 779 LRLLFRVDIINMFQMNKALSRHIWPLDSENVTSTANEKQRNKEKPEATVTSAPKEKRKQE 958
L+ LF V+ INMFQMNKALS+HIWPL+S +V + ++ +K + K+++KQ
Sbjct: 63 LKALFGVNSINMFQMNKALSKHIWPLESNDVIPAKSSQKEKPQKEKQPKEKPQKKRQKQG 122
Query: 959 RHEDDDESQVKEKRQRRAGLGFLAPLPLSDALAKFIGTGESILPRSTVVKRIWDYIKAND 1138
+ ED D+S ++KRQ++ GFLAPLPLS+AL F+GTGE LPRS VVKR+WDYIK N+
Sbjct: 123 KEEDSDDSAREDKRQKKGKSGFLAPLPLSNALVAFLGTGEDALPRSDVVKRMWDYIKQNN 182
Query: 1139 LQDPNDKKMVLCDDKLKELFEVDSFTGFTVPKLLKTHFIKTER 1267
LQDP+DK+ ++CD++LKELF+VDSF GFTV KLL THFIK ++
Sbjct: 183 LQDPSDKRRIICDERLKELFDVDSFNGFTVSKLLATHFIKMKQ 225
>emb|CAN83123.1| hypothetical protein VITISV_044372 [Vitis vinifera]
Length = 332
Score = 290 bits (741), Expect = 8e-76
Identities = 144/231 (62%), Positives = 175/231 (75%)
Frame = +2
Query: 575 SNNKNKDVKRKGNGGGFNKVCSLSPELQQLLGVPELARTEVVKQLWAYIRENDLQDPSNR 754
S NK+VK++G GGF K+CSLSPELQ+ +GVPELARTEVVKQLW +IRE DLQDP+NR
Sbjct: 119 SKKSNKEVKKRG--GGFCKLCSLSPELQKFIGVPELARTEVVKQLWVHIREKDLQDPNNR 176
Query: 755 KNIKCDEPLRLLFRVDIINMFQMNKALSRHIWPLDSENVTSTANEKQRNKEKPEATVTSA 934
+NI CDE LR LF VD INMFQMNKALS+HIWPLDS++ A V
Sbjct: 177 RNIICDETLRALFGVDSINMFQMNKALSKHIWPLDSDDA---------------APVKPT 221
Query: 935 PKEKRKQERHEDDDESQVKEKRQRRAGLGFLAPLPLSDALAKFIGTGESILPRSTVVKRI 1114
PKEK++++ D DE + K+KRQ+ GFLAPLPLSDAL KF+ ES L R+ VVK+I
Sbjct: 222 PKEKQRKQERXDSDEPKRKQKRQKGGNSGFLAPLPLSDALVKFLNIEESALSRAEVVKKI 281
Query: 1115 WDYIKANDLQDPNDKKMVLCDDKLKELFEVDSFTGFTVPKLLKTHFIKTER 1267
W+YIK N+LQDP+DK+ ++CD+KLKELF+VDSF GFTVPKLL HFIKTE+
Sbjct: 282 WEYIKQNNLQDPSDKRRIICDEKLKELFDVDSFNGFTVPKLLSAHFIKTEQ 332
Score = 95.5 bits (236), Expect = 3e-17
Identities = 47/60 (78%), Positives = 53/60 (88%)
Frame = +2
Query: 74 MVSDSELILRLREFLKTSDLNTTTNAIVRRKLEEDFGIDLSDKKAFIRSQVDIFIHSQVE 253
MVSDSEL+ RL EFL++SDLNTTT AIVRRKLEEDFGIDLS KKAFIR VD+F+ SQ+E
Sbjct: 1 MVSDSELVARLHEFLRSSDLNTTTTAIVRRKLEEDFGIDLSGKKAFIREHVDLFLQSQLE 60
Score = 79.3 bits (194), Expect = 2e-12
Identities = 42/93 (45%), Positives = 57/93 (61%), Gaps = 2/93 (2%)
Frame = +2
Query: 572 SSNNKNKDVKRKGNGGGFNKVCSLSPELQQLLGVPE--LARTEVVKQLWAYIRENDLQDP 745
S K K ++KG GF LS L + L + E L+R EVVK++W YI++N+LQDP
Sbjct: 234 SDEPKRKQKRQKGGNSGFLAPLPLSDALVKFLNIEESALSRAEVVKKIWEYIKQNNLQDP 293
Query: 746 SNRKNIKCDEPLRLLFRVDIINMFQMNKALSRH 844
S+++ I CDE L+ LF VD N F + K LS H
Sbjct: 294 SDKRRIICDEKLKELFDVDSFNGFTVPKLLSAH 326
>ref|XP_003537360.1| PREDICTED: uncharacterized protein LOC100786835 [Glycine max]
Length = 346
Score = 286 bits (732), Expect = 9e-75
Identities = 147/233 (63%), Positives = 178/233 (76%), Gaps = 2/233 (0%)
Frame = +2
Query: 575 SNNKNKDVKRKGNGGGFNKVCSLSPELQQLLGVPELARTEVVKQLWAYIRENDLQDPSNR 754
SN +V +K GGGF K+CSLSP+LQ+ +G PE+ARTEVVKQLWAYIRE +LQDP+NR
Sbjct: 130 SNTLGDEVVKK-RGGGFCKLCSLSPQLQEFMGAPEMARTEVVKQLWAYIREKNLQDPNNR 188
Query: 755 KNIKCDEPLRLLFRVDIINMFQMNKALSRHIWPLDSENVTSTANEKQRNKEKPEATVTSA 934
+NI CDE LR LF V+ INMFQMNKALS+HIWPLDS++V V S
Sbjct: 189 RNIICDERLRSLFNVNSINMFQMNKALSKHIWPLDSDDVVQ---------------VKST 233
Query: 935 PKEK-RKQERHEDDDESQVKEKRQRRAG-LGFLAPLPLSDALAKFIGTGESILPRSTVVK 1108
PKEK +KQER +D DE + KEKRQ+ G GFLAPL LSDAL F+GTGES L R+ V+K
Sbjct: 234 PKEKQKKQERDDDSDEPKKKEKRQKGGGKSGFLAPLQLSDALVNFLGTGESELARTDVIK 293
Query: 1109 RIWDYIKANDLQDPNDKKMVLCDDKLKELFEVDSFTGFTVPKLLKTHFIKTER 1267
R+WDYIK N+LQDP+DK+ ++CD+KLKELF+VD+FTGFTV KLL HFIKTE+
Sbjct: 294 RMWDYIKGNNLQDPSDKRKIICDEKLKELFDVDTFTGFTVTKLLAPHFIKTEQ 346
Score = 88.2 bits (217), Expect = 5e-15
Identities = 43/58 (74%), Positives = 52/58 (89%)
Frame = +2
Query: 74 MVSDSELILRLREFLKTSDLNTTTNAIVRRKLEEDFGIDLSDKKAFIRSQVDIFIHSQ 247
MVS+SELI RLREFL++SDLNTTT A VRR+LE DFGIDLSD+KAFIR QVD+F+ ++
Sbjct: 1 MVSESELIGRLREFLRSSDLNTTTTATVRRQLEADFGIDLSDRKAFIREQVDLFLQTE 58
Score = 77.0 bits (188), Expect = 1e-11
Identities = 55/219 (25%), Positives = 103/219 (47%), Gaps = 29/219 (13%)
Frame = +2
Query: 680 LARTEVVKQLWAYIRENDLQDPSN---RKNIKCDEPLRLLFR-------VDIINMFQMNK 829
++ +E++ +L ++R +DL + R+ ++ D + L R VD+ + N+
Sbjct: 2 VSESELIGRLREFLRSSDLNTTTTATVRRQLEADFGIDLSDRKAFIREQVDLFLQTEHNQ 61
Query: 830 ALSRHIWPLDSENVTSTANEKQRNKEKPEATVTSAPKEKRKQERHEDDDESQV-KEKRQR 1006
+ + +++ PE + S KE ++ E+D+E + K K +
Sbjct: 62 SQQEEEEKEEKHQNDHVEEQEEDAPNNPEQSQPSDSKEVTDEDEEEEDEEEEEDKPKHAK 121
Query: 1007 RA------------------GLGFLAPLPLSDALAKFIGTGESILPRSTVVKRIWDYIKA 1132
+A G GF LS L +F+G E + R+ VVK++W YI+
Sbjct: 122 KAKKNKGRSNTLGDEVVKKRGGGFCKLCSLSPQLQEFMGAPE--MARTEVVKQLWAYIRE 179
Query: 1133 NDLQDPNDKKMVLCDDKLKELFEVDSFTGFTVPKLLKTH 1249
+LQDPN+++ ++CD++L+ LF V+S F + K L H
Sbjct: 180 KNLQDPNNRRNIICDERLRSLFNVNSINMFQMNKALSKH 218